Trichoplusia ni (Cabbage looper)
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20889 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7E5WB14|A0A7E5WB14_TRINI pickpocket protein 28 OS=Trichoplusia ni OX=7111 GN=LOC113500897 PE=3 SV=1
MM1 pKa = 7.94 RR2 pKa = 11.84 SNNTVTDD9 pKa = 3.97 SIYY12 pKa = 10.55 AYY14 pKa = 10.64 FNILSKK20 pKa = 10.77 HH21 pKa = 5.59 FSQINYY27 pKa = 9.57 YY28 pKa = 8.82 FICPQIFAYY37 pKa = 9.6 FSWWEE42 pKa = 4.02 TKK44 pKa = 10.21 AIPVPDD50 pKa = 4.75 PSASIPSLTTTSTPTTCATDD70 pKa = 3.21 STTTSEE76 pKa = 4.19 PTTTSDD82 pKa = 4.44 SEE84 pKa = 4.61 STTEE88 pKa = 5.17 AVTTPDD94 pKa = 3.46 PQQTTEE100 pKa = 4.04 EE101 pKa = 4.52 TTTTSPDD108 pKa = 3.18 NSPNTTDD115 pKa = 2.87 TTTTLDD121 pKa = 3.72 PKK123 pKa = 11.41 DD124 pKa = 3.65 NTTEE128 pKa = 4.07 STTTTNEE135 pKa = 4.03 TTPEE139 pKa = 4.13 TTKK142 pKa = 8.48 TTTEE146 pKa = 3.7 ATTCNPEE153 pKa = 4.0 EE154 pKa = 4.28 TTTAATSTTPDD165 pKa = 3.17 ANATTEE171 pKa = 4.15 PPSTADD177 pKa = 3.1 ATATGSTATTPNYY190 pKa = 10.71 SDD192 pKa = 3.3 TTTEE196 pKa = 4.07 TSVNTTDD203 pKa = 3.08 STTNKK208 pKa = 10.2 PEE210 pKa = 4.17 DD211 pKa = 3.62 TSTEE215 pKa = 3.75 ATATSTEE222 pKa = 4.23 SGTTTGATPCSSDD235 pKa = 3.17 ATDD238 pKa = 3.45 TTTVASATTTGTTPSSSNVTDD259 pKa = 3.59 TTLSSATTTEE269 pKa = 3.86 SSTDD273 pKa = 3.34 SSATPCSSDD282 pKa = 2.88 TTTDD286 pKa = 3.17 STVTTTSASPCDD298 pKa = 3.98 IIEE301 pKa = 4.29 TTTDD305 pKa = 2.84 SSATTTSTTPCSSDD319 pKa = 3.06 AVEE322 pKa = 4.48 TTTEE326 pKa = 3.8 ASPNITSSPPSATDD340 pKa = 3.18 PTEE343 pKa = 3.87 PTTDD347 pKa = 3.41 SNASTTSATPCSNDD361 pKa = 3.13 TTEE364 pKa = 4.29 TTPEE368 pKa = 3.95 SSLTTTNAAPRR379 pKa = 11.84 LTDD382 pKa = 3.43 TAEE385 pKa = 4.08 TTTDD389 pKa = 2.98 SSATTTSATSCSSDD403 pKa = 3.04 ASKK406 pKa = 8.09 TTTDD410 pKa = 4.13 SIATTISAAPCVTDD424 pKa = 3.56 TKK426 pKa = 10.64 EE427 pKa = 4.03 TTTDD431 pKa = 3.14 SSATTATATSSEE443 pKa = 4.02 YY444 pKa = 10.9 DD445 pKa = 3.26 ATEE448 pKa = 3.81 KK449 pKa = 8.26 TTEE452 pKa = 4.13 SGVTTTDD459 pKa = 2.97 ATPCLSNATQTTTTSTARR477 pKa = 11.84 TTDD480 pKa = 3.34 ATPCSSDD487 pKa = 3.06 ATEE490 pKa = 4.14 TTTDD494 pKa = 3.02 SSAITTDD501 pKa = 3.26 ATPCASDD508 pKa = 3.26 ATEE511 pKa = 4.09 TTTDD515 pKa = 3.02 SSAITTDD522 pKa = 3.12 ATPYY526 pKa = 10.97 ASDD529 pKa = 3.41 ATEE532 pKa = 4.05 TTTDD536 pKa = 3.02 SSAITTDD543 pKa = 3.26 ATPCASDD550 pKa = 3.26 ATEE553 pKa = 4.09 TTTDD557 pKa = 3.15 SSATTTDD564 pKa = 2.96 ATPCVSDD571 pKa = 3.46 ATEE574 pKa = 4.07 TTTDD578 pKa = 3.02 SSAITTDD585 pKa = 3.26 ATPCASDD592 pKa = 3.26 ATEE595 pKa = 4.09 TTTDD599 pKa = 3.02 SSAITTDD606 pKa = 3.26 ATPCASDD613 pKa = 3.32 ATEE616 pKa = 3.9 TATDD620 pKa = 3.69 SSATPTDD627 pKa = 3.62 ATPCASDD634 pKa = 3.26 ATEE637 pKa = 4.09 TTTDD641 pKa = 3.02 SSAITTDD648 pKa = 3.26 ATPCASDD655 pKa = 3.26 ATEE658 pKa = 4.09 TTTDD662 pKa = 3.02 SSAITTDD669 pKa = 3.26 ATPCASDD676 pKa = 3.32 ATEE679 pKa = 3.9 TATDD683 pKa = 3.67 SSATTTDD690 pKa = 3.11 ATPCASAATEE700 pKa = 4.31 AEE702 pKa = 4.25 SDD704 pKa = 3.84 STTDD708 pKa = 3.14 ATPRR712 pKa = 11.84 ASNGDD717 pKa = 3.41 TRR719 pKa = 11.84 SATSYY724 pKa = 9.92 YY725 pKa = 10.37 RR726 pKa = 11.84 PVGNRR731 pKa = 11.84 GNKK734 pKa = 9.23 LNHH737 pKa = 6.63 ILSKK741 pKa = 10.14 PVKK744 pKa = 10.21 SLVKK748 pKa = 9.86 KK749 pKa = 10.25 LRR751 pKa = 11.84 PILVFLKK758 pKa = 10.49 HH759 pKa = 6.19 LSSLLLLFCVQSSPIEE775 pKa = 4.15 VKK777 pKa = 9.69 MKK779 pKa = 10.49 SYY781 pKa = 9.44 TLILLVLSCLLVFPSDD797 pKa = 3.68 QSALPDD803 pKa = 3.92 ASPCFLGPAFYY814 pKa = 9.79 PRR816 pKa = 11.84 HH817 pKa = 6.1 RR818 pKa = 11.84 EE819 pKa = 3.65 RR820 pKa = 11.84 HH821 pKa = 5.47 RR822 pKa = 11.84 STTTPSTTTSSTSSTSSTTTSTTTSTTSSPQRR854 pKa = 11.84 RR855 pKa = 11.84 RR856 pKa = 11.84 WRR858 pKa = 11.84 RR859 pKa = 11.84 RR860 pKa = 11.84 WFNPFYY866 pKa = 10.05 WWWLL870 pKa = 3.45
Molecular weight: 89.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.682
IPC2_protein 3.732
IPC_protein 3.77
Toseland 3.541
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.126
Thurlkill 3.592
EMBOSS 3.706
Sillero 3.897
Patrickios 1.265
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A7E5VNM7|A0A7E5VNM7_TRINI CREB-regulated transcription coactivator 2-like isoform X1 OS=Trichoplusia ni OX=7111 GN=LOC113495410 PE=3 SV=1
MM1 pKa = 7.67 ASSKK5 pKa = 9.45 TLFILGVLLALVATTFAQNRR25 pKa = 11.84 GRR27 pKa = 11.84 GGFGGPGGFGGPGGNRR43 pKa = 11.84 PGGNRR48 pKa = 11.84 PGGGFGGGNNGFGQGGFGQGGFGGGNNGFGPGGFGGGNNGFGQGGFGGNRR98 pKa = 11.84 PGGFGGPGGFGGPGGFGRR116 pKa = 11.84 PGFGRR121 pKa = 3.64
Molecular weight: 11.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.466
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.29
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.34
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.106
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.143
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14669
6220
20889
13497636
34
20773
646.2
72.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.39 ± 0.027
1.879 ± 0.025
5.628 ± 0.012
6.902 ± 0.033
3.293 ± 0.014
5.655 ± 0.027
2.51 ± 0.009
4.969 ± 0.019
6.353 ± 0.03
8.761 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.165 ± 0.01
4.53 ± 0.017
5.825 ± 0.026
4.156 ± 0.021
5.733 ± 0.021
7.782 ± 0.022
6.081 ± 0.041
6.324 ± 0.019
1.052 ± 0.007
3.009 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here