Acer yangbiense
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 28112 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C7I7V0|A0A5C7I7V0_9ROSI Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_006928 PE=4 SV=1
MM1 pKa = 7.47 YY2 pKa = 7.74 EE3 pKa = 4.11 TNHH6 pKa = 7.35 LEE8 pKa = 4.13 EE9 pKa = 5.15 NPPSAYY15 pKa = 10.46 GQMDD19 pKa = 4.04 SCPGNVCPDD28 pKa = 3.55 FDD30 pKa = 4.72 GNQVFHH36 pKa = 7.44 SPIPEE41 pKa = 4.48 CGASFPNLEE50 pKa = 4.22 YY51 pKa = 10.78 SSPLPEE57 pKa = 3.75 MPIWRR62 pKa = 11.84 TVEE65 pKa = 5.15 DD66 pKa = 4.22 IPSPSIPVDD75 pKa = 3.61 DD76 pKa = 4.07 SHH78 pKa = 9.03 RR79 pKa = 11.84 EE80 pKa = 3.93 EE81 pKa = 5.25 DD82 pKa = 4.13 LHH84 pKa = 7.62 TGDD87 pKa = 5.88 AFALTDD93 pKa = 5.02 DD94 pKa = 4.94 GDD96 pKa = 4.36 AKK98 pKa = 10.58 DD99 pKa = 3.73 TTGGAEE105 pKa = 4.42 GYY107 pKa = 9.82 LAEE110 pKa = 5.29 LSNSLLNFNEE120 pKa = 4.29 EE121 pKa = 4.1 EE122 pKa = 5.08 LMTLDD127 pKa = 4.59 DD128 pKa = 4.4 VEE130 pKa = 5.98 KK131 pKa = 11.14 EE132 pKa = 4.19 MIDD135 pKa = 3.23 KK136 pKa = 11.22 SFFDD140 pKa = 4.58 GLNLLLNSPNDD151 pKa = 3.45 VNQDD155 pKa = 3.39 HH156 pKa = 6.51 MPSPEE161 pKa = 4.35 PEE163 pKa = 3.99 TSVTPDD169 pKa = 3.42 YY170 pKa = 10.84 LANAYY175 pKa = 8.81 GSCPVEE181 pKa = 4.23 SVEE184 pKa = 4.07 NVHH187 pKa = 5.79 MQSSASASDD196 pKa = 3.44 PQFPEE201 pKa = 5.32 LIDD204 pKa = 3.64 GVIYY208 pKa = 8.88 CTLNMEE214 pKa = 5.53 DD215 pKa = 5.25 PDD217 pKa = 4.3 IPCNDD222 pKa = 3.84 DD223 pKa = 3.55 VFFPNKK229 pKa = 9.7 LQPLSVSAVAQRR241 pKa = 11.84 EE242 pKa = 4.47 AGNSTCSSVKK252 pKa = 10.49 DD253 pKa = 3.33 LSGNKK258 pKa = 9.46 NLTDD262 pKa = 4.14 GGPVLLQRR270 pKa = 11.84 EE271 pKa = 4.48 QEE273 pKa = 4.26 NPAQSQVSSQMMGSQVIPEE292 pKa = 4.3 KK293 pKa = 10.97 VQFHH297 pKa = 5.7 PVKK300 pKa = 10.63 FGLPNCDD307 pKa = 3.58 SPHH310 pKa = 5.79 LASRR314 pKa = 11.84 KK315 pKa = 9.39 PGIACGGSNLTNSMSICTDD334 pKa = 2.47 SHH336 pKa = 6.14 QPARR340 pKa = 11.84 LKK342 pKa = 10.81 EE343 pKa = 4.06 EE344 pKa = 4.05 NKK346 pKa = 9.82 EE347 pKa = 3.74 YY348 pKa = 10.49 CYY350 pKa = 10.83 KK351 pKa = 10.8 RR352 pKa = 11.84 NANGVKK358 pKa = 9.59 QEE360 pKa = 4.44 CDD362 pKa = 3.47 DD363 pKa = 4.2 PATIQDD369 pKa = 4.15 CQTLNAEE376 pKa = 4.55 FGSTNIPDD384 pKa = 4.65 LEE386 pKa = 5.25 PDD388 pKa = 3.14 INDD391 pKa = 4.94 PISEE395 pKa = 4.23 PEE397 pKa = 4.0 EE398 pKa = 3.85 PSIEE402 pKa = 4.18 SDD404 pKa = 3.88 DD405 pKa = 3.99 DD406 pKa = 3.61 VPYY409 pKa = 11.11 YY410 pKa = 11.05 SDD412 pKa = 3.72 IEE414 pKa = 4.52 AMILDD419 pKa = 4.49 MDD421 pKa = 5.92 LDD423 pKa = 4.92 PDD425 pKa = 3.87 DD426 pKa = 4.11 QDD428 pKa = 3.6 IYY430 pKa = 9.78 EE431 pKa = 4.36 QEE433 pKa = 3.93 VSKK436 pKa = 10.78 YY437 pKa = 6.6 QHH439 pKa = 6.45 EE440 pKa = 4.16 DD441 pKa = 2.92 TRR443 pKa = 11.84 RR444 pKa = 11.84 GNHH447 pKa = 4.9 QAA449 pKa = 3.52
Molecular weight: 49.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.897
IPC_protein 3.923
Toseland 3.706
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.795
Rodwell 3.745
Grimsley 3.617
Solomon 3.884
Lehninger 3.846
Nozaki 3.999
DTASelect 4.215
Thurlkill 3.745
EMBOSS 3.808
Sillero 4.037
Patrickios 1.227
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.906
Protein with the highest isoelectric point:
>tr|A0A5C7GZY6|A0A5C7GZY6_9ROSI Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_022882 PE=4 SV=1
MM1 pKa = 7.71 PFIKK5 pKa = 9.49 FTKK8 pKa = 9.7 NPSSSSINSTLLISFPLLHH27 pKa = 6.53 SRR29 pKa = 11.84 RR30 pKa = 11.84 PSHH33 pKa = 6.7 LLLRR37 pKa = 11.84 STHH40 pKa = 5.05 GRR42 pKa = 11.84 RR43 pKa = 11.84 PSHH46 pKa = 6.9 RR47 pKa = 11.84 IKK49 pKa = 10.33 IRR51 pKa = 11.84 NRR53 pKa = 11.84 GVV55 pKa = 2.72
Molecular weight: 6.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.096
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.501
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.237
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.129
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
28112
0
28112
12256278
51
5462
436.0
48.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.287 ± 0.014
1.948 ± 0.007
5.362 ± 0.009
6.438 ± 0.016
4.355 ± 0.01
6.374 ± 0.015
2.365 ± 0.006
5.584 ± 0.011
6.294 ± 0.013
9.815 ± 0.018
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.502 ± 0.006
4.737 ± 0.01
4.637 ± 0.015
3.633 ± 0.011
5.052 ± 0.011
8.886 ± 0.015
4.93 ± 0.009
6.636 ± 0.009
1.325 ± 0.005
2.841 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here