Streptomyces kaniharaensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7336 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N7KTP9|A0A6N7KTP9_9ACTN HAD family hydrolase OS=Streptomyces kaniharaensis OX=212423 GN=F7Q99_22160 PE=4 SV=1
MM1 pKa = 7.43PVVTAPYY8 pKa = 10.34KK9 pKa = 10.59PGTPCWIDD17 pKa = 4.43LMASDD22 pKa = 4.32QQAALDD28 pKa = 4.26FYY30 pKa = 11.39RR31 pKa = 11.84DD32 pKa = 3.46LLGWQGEE39 pKa = 4.32VGPAEE44 pKa = 3.85FGGYY48 pKa = 7.65SVCTLNGRR56 pKa = 11.84PVAGIMAQSAPEE68 pKa = 4.22GQPLPPVGWTTYY80 pKa = 10.14LASDD84 pKa = 4.43DD85 pKa = 3.85VDD87 pKa = 3.45ASSRR91 pKa = 11.84AISEE95 pKa = 4.02AGGTILYY102 pKa = 8.39PVTDD106 pKa = 3.42IGTVGRR112 pKa = 11.84MLVATDD118 pKa = 3.24PTGAVFGVWQKK129 pKa = 11.6LDD131 pKa = 4.08FIGAGVVNEE140 pKa = 4.37PGALVWNEE148 pKa = 4.33LNTSSTDD155 pKa = 3.3AAGGFYY161 pKa = 10.36HH162 pKa = 6.48QALGLRR168 pKa = 11.84PATIQGMDD176 pKa = 3.43GYY178 pKa = 11.37YY179 pKa = 10.32SLNVGDD185 pKa = 3.86RR186 pKa = 11.84TVGGMQAVPEE196 pKa = 4.15YY197 pKa = 9.82LAEE200 pKa = 4.26GTPPHH205 pKa = 5.82WMVYY209 pKa = 9.76FSVDD213 pKa = 3.62DD214 pKa = 4.33ADD216 pKa = 3.98STVDD220 pKa = 3.6ALVKK224 pKa = 10.78AGGSVIQPPFDD235 pKa = 3.62MQSGRR240 pKa = 11.84MAVVQDD246 pKa = 3.52PQGAVFAIIEE256 pKa = 4.33SPQAQMPDD264 pKa = 3.44SPP266 pKa = 4.42

Molecular weight: 27.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N7KSM9|A0A6N7KSM9_9ACTN Rhomboid family intramembrane serine protease OS=Streptomyces kaniharaensis OX=212423 GN=F7Q99_13885 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.43GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7336

0

7336

2347241

28

8029

320.0

34.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.223 ± 0.049

0.821 ± 0.008

5.824 ± 0.022

5.547 ± 0.031

2.597 ± 0.02

9.297 ± 0.033

2.369 ± 0.015

3.099 ± 0.021

1.957 ± 0.023

10.631 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.629 ± 0.012

1.751 ± 0.015

6.459 ± 0.03

2.985 ± 0.018

8.123 ± 0.036

4.836 ± 0.022

6.121 ± 0.027

8.191 ± 0.028

1.552 ± 0.013

1.988 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski