Nesidiocoris tenuis
Average proteome isoelectric point is 7.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23052 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6H5HLI2|A0A6H5HLI2_9HEMI Uncharacterized protein OS=Nesidiocoris tenuis OX=355587 GN=NTEN_LOCUS22040 PE=4 SV=1
MM1 pKa = 7.24 SVWKK5 pKa = 10.19 IKK7 pKa = 10.44 RR8 pKa = 11.84 PPSVYY13 pKa = 10.83 NCTCCAIIFNTRR25 pKa = 11.84 ITYY28 pKa = 9.02 LRR30 pKa = 11.84 PFDD33 pKa = 4.94 PNFFDD38 pKa = 5.15 ISSDD42 pKa = 3.42 NCPLVANPDD51 pKa = 3.75 QLDD54 pKa = 3.71 TDD56 pKa = 3.53 QDD58 pKa = 4.01 GADD61 pKa = 4.01 KK62 pKa = 11.2 KK63 pKa = 11.36 GDD65 pKa = 3.66 ACDD68 pKa = 3.64 NCPTVPNMDD77 pKa = 3.67 QDD79 pKa = 5.9 DD80 pKa = 3.81 IDD82 pKa = 4.62 KK83 pKa = 11.29 DD84 pKa = 4.41 GIGDD88 pKa = 3.87 ACDD91 pKa = 3.67 SDD93 pKa = 3.82 MDD95 pKa = 4.3 NDD97 pKa = 4.27 GAA99 pKa = 4.86
Molecular weight: 10.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.681
IPC_protein 3.706
Toseland 3.452
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.757
Rodwell 3.528
Grimsley 3.363
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.228
Thurlkill 3.541
EMBOSS 3.757
Sillero 3.846
Patrickios 1.914
IPC_peptide 3.719
IPC2_peptide 3.808
IPC2.peptide.svr19 3.782
Protein with the highest isoelectric point:
>tr|A0A6H5G410|A0A6H5G410_9HEMI Uncharacterized protein (Fragment) OS=Nesidiocoris tenuis OX=355587 GN=NTEN_LOCUS3292 PE=4 SV=1
MM1 pKa = 7.59 RR2 pKa = 11.84 WRR4 pKa = 11.84 RR5 pKa = 11.84 SIMRR9 pKa = 11.84 GSRR12 pKa = 11.84 MIMRR16 pKa = 11.84 WRR18 pKa = 11.84 RR19 pKa = 11.84 SIMRR23 pKa = 11.84 WGRR26 pKa = 11.84 RR27 pKa = 11.84 IIMRR31 pKa = 11.84 CIMKK35 pKa = 9.39 WRR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 SIMRR43 pKa = 11.84 WRR45 pKa = 11.84 GRR47 pKa = 11.84 RR48 pKa = 11.84 GTRR51 pKa = 11.84 MKK53 pKa = 10.08 RR54 pKa = 11.84 RR55 pKa = 11.84 RR56 pKa = 11.84 RR57 pKa = 11.84 RR58 pKa = 11.84 RR59 pKa = 11.84 SIMM62 pKa = 3.39
Molecular weight: 8.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.618
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.325
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.252
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
23052
0
23052
7171166
49
6545
311.1
35.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.337 ± 0.02
1.993 ± 0.016
4.89 ± 0.015
6.001 ± 0.024
4.212 ± 0.022
5.66 ± 0.019
2.555 ± 0.012
5.208 ± 0.015
6.022 ± 0.022
8.807 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.142 ± 0.009
4.508 ± 0.016
5.841 ± 0.027
4.116 ± 0.019
7.06 ± 0.025
8.906 ± 0.032
5.807 ± 0.02
6.017 ± 0.017
1.19 ± 0.006
2.687 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here