Prevotella timonensis CRIS 5C-B1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; Prevotella timonensis

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2202 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1VYC8|D1VYC8_9BACT Dihydrodipicolinate synthetase family OS=Prevotella timonensis CRIS 5C-B1 OX=679189 GN=HMPREF9019_1651 PE=3 SV=1
MM1 pKa = 7.19YY2 pKa = 7.77WTLEE6 pKa = 4.11LASKK10 pKa = 11.0LEE12 pKa = 4.24DD13 pKa = 4.06APWPATKK20 pKa = 10.54EE21 pKa = 3.89EE22 pKa = 4.86LIDD25 pKa = 3.48YY26 pKa = 8.58AIRR29 pKa = 11.84SGAPLEE35 pKa = 4.08VLEE38 pKa = 4.32NLQEE42 pKa = 4.71IEE44 pKa = 4.95DD45 pKa = 4.04EE46 pKa = 4.42GDD48 pKa = 3.53VYY50 pKa = 11.64DD51 pKa = 5.01SIEE54 pKa = 5.25DD55 pKa = 3.27IWPDD59 pKa = 3.64YY60 pKa = 7.51PTKK63 pKa = 10.83EE64 pKa = 3.75DD65 pKa = 3.64FLFNEE70 pKa = 4.91DD71 pKa = 3.49EE72 pKa = 4.45YY73 pKa = 12.0

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1W242|D1W242_9BACT RibD_C domain-containing protein OS=Prevotella timonensis CRIS 5C-B1 OX=679189 GN=HMPREF9019_0236 PE=4 SV=1
MM1 pKa = 7.59RR2 pKa = 11.84CSLRR6 pKa = 11.84IRR8 pKa = 11.84WRR10 pKa = 11.84VRR12 pKa = 11.84VLTLSRR18 pKa = 11.84CAASRR23 pKa = 11.84VVSNLSGDD31 pKa = 3.73AALLSPEE38 pKa = 4.2TARR41 pKa = 11.84TCAAISAICSVNDD54 pKa = 3.43RR55 pKa = 11.84KK56 pKa = 8.98TEE58 pKa = 3.86LSIVITFIFSPFFLYY73 pKa = 10.46LRR75 pKa = 11.84QNSAINKK82 pKa = 9.24RR83 pKa = 11.84GFNNSLRR90 pKa = 11.84DD91 pKa = 3.34SRR93 pKa = 11.84KK94 pKa = 9.37IFTEE98 pKa = 4.3DD99 pKa = 3.28GSVTRR104 pKa = 11.84SNGATGSLSVEE115 pKa = 4.15NGTCPGVYY123 pKa = 9.39RR124 pKa = 11.84YY125 pKa = 10.7LCGQTDD131 pKa = 2.99NCMNMEE137 pKa = 3.97IVSIEE142 pKa = 4.01KK143 pKa = 8.92KK144 pKa = 8.15TFEE147 pKa = 4.12MMVATFGALSEE158 pKa = 4.3KK159 pKa = 10.27VAALRR164 pKa = 11.84RR165 pKa = 11.84KK166 pKa = 9.81SDD168 pKa = 3.25TGRR171 pKa = 11.84MEE173 pKa = 3.62RR174 pKa = 11.84WLTGEE179 pKa = 4.23EE180 pKa = 4.18VCGQLRR186 pKa = 11.84ISPRR190 pKa = 11.84TLQTLRR196 pKa = 11.84DD197 pKa = 3.68RR198 pKa = 11.84RR199 pKa = 11.84LIGYY203 pKa = 7.31SQINRR208 pKa = 11.84RR209 pKa = 11.84FYY211 pKa = 10.99YY212 pKa = 10.25KK213 pKa = 10.2PEE215 pKa = 3.83EE216 pKa = 4.27VKK218 pKa = 10.77RR219 pKa = 11.84LIPLIGTLYY228 pKa = 9.28PHH230 pKa = 7.04GRR232 pKa = 3.54

Molecular weight: 26.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2202

0

2202

784895

32

2529

356.4

40.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.926 ± 0.046

1.225 ± 0.019

5.586 ± 0.031

6.106 ± 0.052

4.409 ± 0.03

6.512 ± 0.044

2.239 ± 0.026

6.753 ± 0.044

6.947 ± 0.05

9.007 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.863 ± 0.024

5.235 ± 0.041

3.63 ± 0.025

4.22 ± 0.038

4.62 ± 0.031

5.967 ± 0.039

5.761 ± 0.034

6.425 ± 0.035

1.22 ± 0.02

4.349 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski