Microbacterium oleivorans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae;

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2781 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A031FRG5|A0A031FRG5_9MICO Putative N-acetylglucosamine kinase OS=Microbacterium oleivorans OX=273677 GN=BW34_02517 PE=4 SV=1
MM1 pKa = 6.72TVRR4 pKa = 11.84RR5 pKa = 11.84ALPLLAATALLLTGCAGQSPPPRR28 pKa = 11.84YY29 pKa = 9.81LPGGPQVPPAGAVLDD44 pKa = 4.09YY45 pKa = 10.98QLGGAYY51 pKa = 9.78DD52 pKa = 3.82PPNGVEE58 pKa = 3.64IVVRR62 pKa = 11.84DD63 pKa = 3.83RR64 pKa = 11.84AAASIDD70 pKa = 3.47DD71 pKa = 4.9LYY73 pKa = 11.49SVCYY77 pKa = 10.73VNGFQTQPGEE87 pKa = 4.08LDD89 pKa = 3.4VWPDD93 pKa = 3.57DD94 pKa = 4.49LLLRR98 pKa = 11.84DD99 pKa = 4.64DD100 pKa = 5.23DD101 pKa = 4.67GEE103 pKa = 4.24PVIDD107 pKa = 4.83PDD109 pKa = 3.66WPDD112 pKa = 3.38EE113 pKa = 4.44VILDD117 pKa = 3.63TRR119 pKa = 11.84KK120 pKa = 9.98ADD122 pKa = 4.37EE123 pKa = 3.86IAKK126 pKa = 10.03IVGPWIRR133 pKa = 11.84DD134 pKa = 3.61CASDD138 pKa = 3.96GFDD141 pKa = 4.14AVEE144 pKa = 4.15FDD146 pKa = 4.79NLDD149 pKa = 3.37TYY151 pKa = 11.1TRR153 pKa = 11.84TDD155 pKa = 3.23GALSRR160 pKa = 11.84DD161 pKa = 3.95DD162 pKa = 5.25ALDD165 pKa = 3.86LATLLVDD172 pKa = 3.84TAHH175 pKa = 7.38DD176 pKa = 3.67VGLAAGQKK184 pKa = 9.41NAAEE188 pKa = 4.54DD189 pKa = 3.51ASLLHH194 pKa = 6.29SRR196 pKa = 11.84AGFDD200 pKa = 3.23FAVVEE205 pKa = 4.28EE206 pKa = 4.37CAAYY210 pKa = 9.6EE211 pKa = 3.87EE212 pKa = 4.77CAAYY216 pKa = 9.47TEE218 pKa = 4.81VYY220 pKa = 10.04GEE222 pKa = 3.99NVLAIEE228 pKa = 4.38YY229 pKa = 8.94TDD231 pKa = 3.54NLPRR235 pKa = 11.84PWDD238 pKa = 3.79EE239 pKa = 4.0VCADD243 pKa = 3.98PEE245 pKa = 4.58TPASVVLRR253 pKa = 11.84DD254 pKa = 3.9RR255 pKa = 11.84DD256 pKa = 4.02LVTPDD261 pKa = 3.22DD262 pKa = 4.29PGYY265 pKa = 11.15VLEE268 pKa = 4.56TCGG271 pKa = 4.4

Molecular weight: 29.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A031FTN2|A0A031FTN2_9MICO Cysteine desulfurase OS=Microbacterium oleivorans OX=273677 GN=BW34_02204 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2781

0

2781

897455

29

2036

322.7

34.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.58 ± 0.067

0.445 ± 0.011

6.544 ± 0.045

5.566 ± 0.042

3.145 ± 0.031

8.887 ± 0.039

1.939 ± 0.024

4.548 ± 0.038

1.987 ± 0.031

9.909 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.762 ± 0.017

1.841 ± 0.024

5.381 ± 0.035

2.612 ± 0.023

7.451 ± 0.051

5.627 ± 0.034

6.11 ± 0.036

9.2 ± 0.04

1.532 ± 0.022

1.934 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski