Aphanothece hegewaldii CCALA 016

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Aphanothecaceae; Aphanothece; Aphanothece hegewaldii

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4827 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T1LX15|A0A2T1LX15_9CHRO Transposase OS=Aphanothece hegewaldii CCALA 016 OX=2107694 GN=C7H19_13395 PE=3 SV=1
MM1 pKa = 7.49RR2 pKa = 11.84RR3 pKa = 11.84IGTIGPDD10 pKa = 3.26NLLGTSEE17 pKa = 4.96DD18 pKa = 5.04DD19 pKa = 3.17ILKK22 pKa = 10.25GLKK25 pKa = 10.67GNDD28 pKa = 3.18VLRR31 pKa = 11.84GLKK34 pKa = 10.4GDD36 pKa = 4.26DD37 pKa = 3.98LLFGGDD43 pKa = 3.91GNDD46 pKa = 3.42TIYY49 pKa = 11.16AGGGNDD55 pKa = 3.46KK56 pKa = 11.21VIGGEE61 pKa = 4.32GNDD64 pKa = 3.72FLYY67 pKa = 10.73KK68 pKa = 10.07ILSGKK73 pKa = 5.61TTAVNFSYY81 pKa = 10.92TNPEE85 pKa = 3.55VGKK88 pKa = 9.57IQGIEE93 pKa = 4.02SVYY96 pKa = 10.87LSTGLGNDD104 pKa = 4.23TIISTAAANTIYY116 pKa = 11.18SNDD119 pKa = 3.38GNDD122 pKa = 3.85WIMTGAGRR130 pKa = 11.84DD131 pKa = 4.0SINSGAGDD139 pKa = 4.23DD140 pKa = 4.64YY141 pKa = 11.19ISSGAGDD148 pKa = 3.95DD149 pKa = 4.77QIDD152 pKa = 3.57AGKK155 pKa = 11.22GNDD158 pKa = 3.66TVIGGAGNDD167 pKa = 3.47SLYY170 pKa = 11.04KK171 pKa = 10.65DD172 pKa = 5.13FSDD175 pKa = 3.24QTTGINFSYY184 pKa = 10.67TNPEE188 pKa = 3.6VGNIQGIEE196 pKa = 4.03AVTLSTGSGDD206 pKa = 3.55DD207 pKa = 4.06TIVLTAAEE215 pKa = 5.23GYY217 pKa = 9.93LYY219 pKa = 10.12HH220 pKa = 7.32KK221 pKa = 10.73NYY223 pKa = 10.08IYY225 pKa = 11.27SNDD228 pKa = 3.5GNNSITTGAGNEE240 pKa = 4.09QIISGAGDD248 pKa = 3.94DD249 pKa = 4.76YY250 pKa = 11.26ISSGAGDD257 pKa = 4.79DD258 pKa = 4.41YY259 pKa = 11.37ISSGTGNDD267 pKa = 3.49TVIGGAGNDD276 pKa = 3.56HH277 pKa = 7.27LYY279 pKa = 11.09KK280 pKa = 10.79DD281 pKa = 5.12FSDD284 pKa = 3.31QTTGINFSYY293 pKa = 10.65TDD295 pKa = 3.93PNVGNIQGIEE305 pKa = 4.1SLHH308 pKa = 6.57LSTGSGDD315 pKa = 3.59DD316 pKa = 4.24TIVSTAAVSLFSVYY330 pKa = 11.02SNYY333 pKa = 9.7ISSNGGNDD341 pKa = 3.96SITTGAGDD349 pKa = 4.35DD350 pKa = 3.92IIYY353 pKa = 10.62AGTGDD358 pKa = 3.6DD359 pKa = 3.7TVIGGEE365 pKa = 4.2GNDD368 pKa = 3.57YY369 pKa = 10.69LGKK372 pKa = 10.35SFSDD376 pKa = 3.19LTTAVNFSYY385 pKa = 10.88TDD387 pKa = 3.73PNSGKK392 pKa = 9.21IQGIEE397 pKa = 3.77RR398 pKa = 11.84LYY400 pKa = 11.1LSTGSGDD407 pKa = 3.56DD408 pKa = 4.24TIVSTAAVGFSDD420 pKa = 3.94YY421 pKa = 10.84SANEE425 pKa = 3.82IYY427 pKa = 11.02SNGGNDD433 pKa = 5.22SITTSAGNDD442 pKa = 3.83YY443 pKa = 10.89INSGAGNDD451 pKa = 4.85GIDD454 pKa = 4.13AGTGDD459 pKa = 3.83DD460 pKa = 3.82TVIGGEE466 pKa = 4.22GNDD469 pKa = 3.74YY470 pKa = 9.32LTKK473 pKa = 10.76DD474 pKa = 3.78FSNLTRR480 pKa = 11.84AVNFSYY486 pKa = 10.89TDD488 pKa = 3.73PNSGKK493 pKa = 9.21IQGIEE498 pKa = 3.77RR499 pKa = 11.84LYY501 pKa = 11.03LSTGAGDD508 pKa = 3.8DD509 pKa = 4.4TIVSAAVGFSNYY521 pKa = 9.28SANEE525 pKa = 3.73IYY527 pKa = 10.91SNEE530 pKa = 3.99GNDD533 pKa = 5.14SITTSAGDD541 pKa = 3.79DD542 pKa = 4.26FISSGAGNDD551 pKa = 4.06SITTGAGDD559 pKa = 3.94DD560 pKa = 4.87DD561 pKa = 4.74IYY563 pKa = 11.6SGAGNDD569 pKa = 4.7LIKK572 pKa = 10.17TGAGNDD578 pKa = 4.3GIIAGEE584 pKa = 4.32GNDD587 pKa = 3.86TLIGGLGNDD596 pKa = 3.81KK597 pKa = 9.93LTGNSGQDD605 pKa = 2.65QFVFNTPTEE614 pKa = 4.63GIDD617 pKa = 3.44RR618 pKa = 11.84IYY620 pKa = 11.04DD621 pKa = 3.7FSLIDD626 pKa = 3.94DD627 pKa = 5.31KK628 pKa = 11.45IAVSRR633 pKa = 11.84SGFSNDD639 pKa = 4.31LIAGAAITVAQFTIDD654 pKa = 3.55SVATTASQRR663 pKa = 11.84FIYY666 pKa = 10.48NSASGALFFDD676 pKa = 4.33ADD678 pKa = 4.35GVGSTAAIQFATLDD692 pKa = 3.54SGLALTNADD701 pKa = 3.29IFVTPP706 pKa = 4.59

Molecular weight: 72.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T1M2L3|A0A2T1M2L3_9CHRO Haloacid dehalogenase OS=Aphanothece hegewaldii CCALA 016 OX=2107694 GN=C7H19_02655 PE=4 SV=1
MM1 pKa = 8.03PINIRR6 pKa = 11.84RR7 pKa = 11.84CISCHH12 pKa = 6.11KK13 pKa = 9.63IAPKK17 pKa = 9.45TSFWRR22 pKa = 11.84IVRR25 pKa = 11.84VYY27 pKa = 10.54PSGQVQLDD35 pKa = 3.34QGMGRR40 pKa = 11.84SAYY43 pKa = 10.44LCPTDD48 pKa = 4.23SCLKK52 pKa = 10.42NALSKK57 pKa = 11.01NRR59 pKa = 11.84LGRR62 pKa = 11.84ALRR65 pKa = 11.84SPIPQDD71 pKa = 3.17LVQTLRR77 pKa = 11.84EE78 pKa = 3.93RR79 pKa = 11.84LINN82 pKa = 3.8

Molecular weight: 9.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4827

0

4827

1539476

23

6380

318.9

35.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.011 ± 0.033

0.942 ± 0.014

4.993 ± 0.034

6.396 ± 0.047

4.087 ± 0.027

6.402 ± 0.053

1.754 ± 0.019

7.577 ± 0.033

5.314 ± 0.038

11.141 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.792 ± 0.019

4.851 ± 0.053

4.468 ± 0.024

5.337 ± 0.037

4.639 ± 0.032

6.577 ± 0.031

5.973 ± 0.051

5.993 ± 0.027

1.4 ± 0.016

3.354 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski