Escherichia phage vB_EcoS_fTaEco01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Kagunavirus; unclassified Kagunavirus

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9VZU3|A0A7G9VZU3_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_fTaEco01 OX=2762430 GN=TE1_042 PE=4 SV=1
MM1 pKa = 7.13NWVSVNEE8 pKa = 4.29ALPEE12 pKa = 4.32SKK14 pKa = 10.37DD15 pKa = 3.7DD16 pKa = 3.86SVLVCSVDD24 pKa = 3.04GGKK27 pKa = 10.35YY28 pKa = 10.03DD29 pKa = 5.26DD30 pKa = 4.33NGFPEE35 pKa = 4.53GGIDD39 pKa = 3.59FVHH42 pKa = 6.27IQDD45 pKa = 4.0YY46 pKa = 10.83FDD48 pKa = 6.61DD49 pKa = 3.76ITAGLDD55 pKa = 3.29EE56 pKa = 5.5NGNQLYY62 pKa = 7.47TKK64 pKa = 9.84RR65 pKa = 11.84YY66 pKa = 7.78IEE68 pKa = 3.98MGITHH73 pKa = 7.23WMYY76 pKa = 11.07LPEE79 pKa = 5.28LPEE82 pKa = 4.02GTKK85 pKa = 10.5

Molecular weight: 9.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9VZW2|A0A7G9VZW2_9CAUD Class II holin-like protein OS=Escherichia phage vB_EcoS_fTaEco01 OX=2762430 GN=TE1_061 PE=4 SV=1
MM1 pKa = 7.52SLATDD6 pKa = 3.35ILKK9 pKa = 10.63RR10 pKa = 11.84SGLAPLSPRR19 pKa = 11.84AKK21 pKa = 7.67TQIYY25 pKa = 9.4KK26 pKa = 10.3RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84NALYY33 pKa = 10.01PEE35 pKa = 3.85IQARR39 pKa = 11.84RR40 pKa = 11.84KK41 pKa = 10.01AISACGFQNGKK52 pKa = 9.72AVNLGEE58 pKa = 4.7FKK60 pKa = 9.58TQEE63 pKa = 3.57RR64 pKa = 11.84AAIANRR70 pKa = 11.84LFNYY74 pKa = 7.62WKK76 pKa = 10.65SLGYY80 pKa = 10.66DD81 pKa = 4.56DD82 pKa = 6.25IPTKK86 pKa = 8.88PQRR89 pKa = 11.84RR90 pKa = 11.84QYY92 pKa = 9.93IWRR95 pKa = 11.84HH96 pKa = 4.21KK97 pKa = 10.55

Molecular weight: 11.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

13467

29

984

184.5

20.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.787 ± 0.519

1.144 ± 0.15

6.2 ± 0.222

6.832 ± 0.412

3.988 ± 0.17

7.507 ± 0.222

1.819 ± 0.183

5.324 ± 0.184

5.955 ± 0.367

7.76 ± 0.307

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.45 ± 0.154

4.381 ± 0.238

3.832 ± 0.207

3.876 ± 0.352

5.346 ± 0.252

5.851 ± 0.392

6.044 ± 0.394

7.002 ± 0.284

1.448 ± 0.163

3.445 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski