Amino acid dipepetide frequency for Escherichia phage vB_EcoS_fTaEco01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.842AlaAla: 10.842 ± 1.706
1.262AlaCys: 1.262 ± 0.336
6.758AlaAsp: 6.758 ± 0.696
6.981AlaGlu: 6.981 ± 0.878
4.456AlaPhe: 4.456 ± 0.643
7.352AlaGly: 7.352 ± 0.837
1.188AlaHis: 1.188 ± 0.31
5.792AlaIle: 5.792 ± 0.818
6.461AlaLys: 6.461 ± 0.674
8.689AlaLeu: 8.689 ± 0.809
2.005AlaMet: 2.005 ± 0.427
3.565AlaAsn: 3.565 ± 0.531
3.713AlaPro: 3.713 ± 0.485
2.97AlaGln: 2.97 ± 0.682
3.862AlaArg: 3.862 ± 0.47
5.347AlaSer: 5.347 ± 0.752
4.456AlaThr: 4.456 ± 0.673
7.129AlaVal: 7.129 ± 0.743
1.857AlaTrp: 1.857 ± 0.348
3.193AlaTyr: 3.193 ± 0.401
0.0AlaXaa: 0.0 ± 0.0
Cys
1.188CysAla: 1.188 ± 0.302
0.074CysCys: 0.074 ± 0.083
0.891CysAsp: 0.891 ± 0.231
1.114CysGlu: 1.114 ± 0.358
0.149CysPhe: 0.149 ± 0.099
1.337CysGly: 1.337 ± 0.332
0.223CysHis: 0.223 ± 0.138
0.371CysIle: 0.371 ± 0.188
0.446CysLys: 0.446 ± 0.175
0.52CysLeu: 0.52 ± 0.25
0.074CysMet: 0.074 ± 0.063
0.371CysAsn: 0.371 ± 0.162
0.371CysPro: 0.371 ± 0.157
0.297CysGln: 0.297 ± 0.134
0.965CysArg: 0.965 ± 0.38
0.743CysSer: 0.743 ± 0.266
0.891CysThr: 0.891 ± 0.31
0.743CysVal: 0.743 ± 0.205
0.149CysTrp: 0.149 ± 0.095
0.52CysTyr: 0.52 ± 0.19
0.0CysXaa: 0.0 ± 0.0
Asp
6.683AspAla: 6.683 ± 0.784
0.743AspCys: 0.743 ± 0.252
5.198AspAsp: 5.198 ± 0.62
4.753AspGlu: 4.753 ± 0.599
2.525AspPhe: 2.525 ± 0.34
6.461AspGly: 6.461 ± 0.936
0.891AspHis: 0.891 ± 0.242
4.159AspIle: 4.159 ± 0.514
3.416AspLys: 3.416 ± 0.435
4.381AspLeu: 4.381 ± 0.663
1.262AspMet: 1.262 ± 0.282
2.376AspAsn: 2.376 ± 0.493
1.262AspPro: 1.262 ± 0.351
1.337AspGln: 1.337 ± 0.304
2.673AspArg: 2.673 ± 0.496
2.599AspSer: 2.599 ± 0.454
3.49AspThr: 3.49 ± 0.458
4.01AspVal: 4.01 ± 0.533
0.891AspTrp: 0.891 ± 0.222
2.896AspTyr: 2.896 ± 0.354
0.0AspXaa: 0.0 ± 0.0
Glu
5.57GluAla: 5.57 ± 0.65
0.446GluCys: 0.446 ± 0.249
3.787GluAsp: 3.787 ± 0.678
5.644GluGlu: 5.644 ± 1.005
2.673GluPhe: 2.673 ± 0.483
4.381GluGly: 4.381 ± 0.537
1.114GluHis: 1.114 ± 0.281
3.49GluIle: 3.49 ± 0.508
4.53GluLys: 4.53 ± 0.724
6.089GluLeu: 6.089 ± 0.567
2.673GluMet: 2.673 ± 0.471
2.228GluAsn: 2.228 ± 0.442
2.005GluPro: 2.005 ± 0.439
3.639GluGln: 3.639 ± 0.785
4.307GluArg: 4.307 ± 0.605
3.193GluSer: 3.193 ± 0.493
3.49GluThr: 3.49 ± 0.583
4.753GluVal: 4.753 ± 0.565
1.411GluTrp: 1.411 ± 0.343
2.822GluTyr: 2.822 ± 0.473
0.0GluXaa: 0.0 ± 0.0
Phe
2.673PheAla: 2.673 ± 0.452
0.668PheCys: 0.668 ± 0.213
3.639PheAsp: 3.639 ± 0.594
2.599PheGlu: 2.599 ± 0.473
1.114PhePhe: 1.114 ± 0.287
3.49PheGly: 3.49 ± 0.499
0.52PheHis: 0.52 ± 0.191
2.822PheIle: 2.822 ± 0.468
2.525PheLys: 2.525 ± 0.431
2.154PheLeu: 2.154 ± 0.441
0.743PheMet: 0.743 ± 0.189
1.857PheAsn: 1.857 ± 0.344
1.559PhePro: 1.559 ± 0.434
0.965PheGln: 0.965 ± 0.251
1.857PheArg: 1.857 ± 0.324
3.267PheSer: 3.267 ± 0.403
2.525PheThr: 2.525 ± 0.392
2.896PheVal: 2.896 ± 0.44
0.668PheTrp: 0.668 ± 0.214
1.337PheTyr: 1.337 ± 0.266
0.0PheXaa: 0.0 ± 0.0
Gly
6.758GlyAla: 6.758 ± 0.847
1.262GlyCys: 1.262 ± 0.288
4.678GlyAsp: 4.678 ± 0.771
5.124GlyGlu: 5.124 ± 0.642
3.416GlyPhe: 3.416 ± 0.559
5.421GlyGly: 5.421 ± 0.73
1.782GlyHis: 1.782 ± 0.413
3.045GlyIle: 3.045 ± 0.484
5.273GlyLys: 5.273 ± 0.776
5.495GlyLeu: 5.495 ± 0.571
2.154GlyMet: 2.154 ± 0.49
3.639GlyAsn: 3.639 ± 0.432
1.708GlyPro: 1.708 ± 0.314
3.49GlyGln: 3.49 ± 0.656
3.342GlyArg: 3.342 ± 0.534
4.901GlySer: 4.901 ± 0.681
4.233GlyThr: 4.233 ± 0.598
5.941GlyVal: 5.941 ± 0.826
1.04GlyTrp: 1.04 ± 0.225
2.376GlyTyr: 2.376 ± 0.43
0.0GlyXaa: 0.0 ± 0.0
His
1.188HisAla: 1.188 ± 0.218
0.446HisCys: 0.446 ± 0.144
1.04HisAsp: 1.04 ± 0.287
0.965HisGlu: 0.965 ± 0.26
0.743HisPhe: 0.743 ± 0.214
1.485HisGly: 1.485 ± 0.325
0.668HisHis: 0.668 ± 0.231
1.114HisIle: 1.114 ± 0.24
1.188HisLys: 1.188 ± 0.383
1.708HisLeu: 1.708 ± 0.403
0.371HisMet: 0.371 ± 0.169
0.743HisAsn: 0.743 ± 0.241
0.965HisPro: 0.965 ± 0.274
0.594HisGln: 0.594 ± 0.166
1.262HisArg: 1.262 ± 0.322
0.594HisSer: 0.594 ± 0.195
0.743HisThr: 0.743 ± 0.32
1.485HisVal: 1.485 ± 0.368
0.297HisTrp: 0.297 ± 0.137
0.594HisTyr: 0.594 ± 0.248
0.0HisXaa: 0.0 ± 0.0
Ile
5.124IleAla: 5.124 ± 0.603
0.891IleCys: 0.891 ± 0.283
3.639IleAsp: 3.639 ± 0.428
3.787IleGlu: 3.787 ± 0.422
1.559IlePhe: 1.559 ± 0.327
2.599IleGly: 2.599 ± 0.445
0.446IleHis: 0.446 ± 0.192
2.896IleIle: 2.896 ± 0.329
3.342IleLys: 3.342 ± 0.542
2.97IleLeu: 2.97 ± 0.441
0.965IleMet: 0.965 ± 0.252
2.822IleAsn: 2.822 ± 0.455
2.748IlePro: 2.748 ± 0.4
2.005IleGln: 2.005 ± 0.392
2.005IleArg: 2.005 ± 0.365
3.936IleSer: 3.936 ± 0.534
4.753IleThr: 4.753 ± 0.742
4.307IleVal: 4.307 ± 0.458
0.891IleTrp: 0.891 ± 0.263
1.559IleTyr: 1.559 ± 0.4
0.0IleXaa: 0.0 ± 0.0
Lys
6.461LysAla: 6.461 ± 0.961
0.446LysCys: 0.446 ± 0.205
3.342LysAsp: 3.342 ± 0.636
4.827LysGlu: 4.827 ± 0.768
2.079LysPhe: 2.079 ± 0.279
3.565LysGly: 3.565 ± 0.48
1.485LysHis: 1.485 ± 0.283
2.302LysIle: 2.302 ± 0.467
3.49LysLys: 3.49 ± 0.521
4.901LysLeu: 4.901 ± 0.565
3.045LysMet: 3.045 ± 0.489
2.005LysAsn: 2.005 ± 0.456
2.673LysPro: 2.673 ± 0.479
1.931LysGln: 1.931 ± 0.445
4.159LysArg: 4.159 ± 0.503
2.525LysSer: 2.525 ± 0.382
3.787LysThr: 3.787 ± 0.533
3.416LysVal: 3.416 ± 0.567
0.594LysTrp: 0.594 ± 0.166
2.525LysTyr: 2.525 ± 0.372
0.0LysXaa: 0.0 ± 0.0
Leu
8.614LeuAla: 8.614 ± 0.8
0.817LeuCys: 0.817 ± 0.256
3.936LeuAsp: 3.936 ± 0.631
4.753LeuGlu: 4.753 ± 0.738
3.119LeuPhe: 3.119 ± 0.665
5.644LeuGly: 5.644 ± 0.563
1.559LeuHis: 1.559 ± 0.418
4.307LeuIle: 4.307 ± 0.45
3.787LeuLys: 3.787 ± 0.594
5.421LeuLeu: 5.421 ± 0.693
1.634LeuMet: 1.634 ± 0.29
4.307LeuAsn: 4.307 ± 0.6
4.307LeuPro: 4.307 ± 0.741
2.896LeuGln: 2.896 ± 0.515
4.233LeuArg: 4.233 ± 0.574
4.381LeuSer: 4.381 ± 0.57
5.421LeuThr: 5.421 ± 0.762
4.53LeuVal: 4.53 ± 0.599
1.04LeuTrp: 1.04 ± 0.279
2.896LeuTyr: 2.896 ± 0.489
0.0LeuXaa: 0.0 ± 0.0
Met
2.748MetAla: 2.748 ± 0.452
0.446MetCys: 0.446 ± 0.159
0.594MetAsp: 0.594 ± 0.252
1.188MetGlu: 1.188 ± 0.313
0.891MetPhe: 0.891 ± 0.244
2.079MetGly: 2.079 ± 0.323
0.223MetHis: 0.223 ± 0.124
1.634MetIle: 1.634 ± 0.455
2.005MetLys: 2.005 ± 0.393
1.782MetLeu: 1.782 ± 0.332
0.52MetMet: 0.52 ± 0.195
0.891MetAsn: 0.891 ± 0.249
1.411MetPro: 1.411 ± 0.263
0.817MetGln: 0.817 ± 0.248
1.411MetArg: 1.411 ± 0.232
2.079MetSer: 2.079 ± 0.252
1.559MetThr: 1.559 ± 0.325
1.708MetVal: 1.708 ± 0.384
0.297MetTrp: 0.297 ± 0.137
0.223MetTyr: 0.223 ± 0.113
0.0MetXaa: 0.0 ± 0.0
Asn
4.604AsnAla: 4.604 ± 0.463
0.594AsnCys: 0.594 ± 0.192
2.673AsnAsp: 2.673 ± 0.376
2.525AsnGlu: 2.525 ± 0.408
1.188AsnPhe: 1.188 ± 0.258
4.307AsnGly: 4.307 ± 0.647
0.817AsnHis: 0.817 ± 0.251
1.931AsnIle: 1.931 ± 0.439
1.857AsnLys: 1.857 ± 0.29
2.822AsnLeu: 2.822 ± 0.407
0.891AsnMet: 0.891 ± 0.274
2.451AsnAsn: 2.451 ± 0.446
1.782AsnPro: 1.782 ± 0.273
1.634AsnGln: 1.634 ± 0.38
2.822AsnArg: 2.822 ± 0.499
2.896AsnSer: 2.896 ± 0.356
2.525AsnThr: 2.525 ± 0.421
3.565AsnVal: 3.565 ± 0.457
0.817AsnTrp: 0.817 ± 0.209
1.114AsnTyr: 1.114 ± 0.257
0.0AsnXaa: 0.0 ± 0.0
Pro
3.267ProAla: 3.267 ± 0.387
0.52ProCys: 0.52 ± 0.166
3.045ProAsp: 3.045 ± 0.452
3.565ProGlu: 3.565 ± 0.431
1.931ProPhe: 1.931 ± 0.388
2.896ProGly: 2.896 ± 0.48
0.594ProHis: 0.594 ± 0.219
1.559ProIle: 1.559 ± 0.359
1.337ProLys: 1.337 ± 0.315
2.896ProLeu: 2.896 ± 0.532
0.817ProMet: 0.817 ± 0.262
1.782ProAsn: 1.782 ± 0.356
0.891ProPro: 0.891 ± 0.291
1.188ProGln: 1.188 ± 0.202
1.559ProArg: 1.559 ± 0.361
2.525ProSer: 2.525 ± 0.398
2.451ProThr: 2.451 ± 0.442
4.01ProVal: 4.01 ± 0.548
0.371ProTrp: 0.371 ± 0.169
1.114ProTyr: 1.114 ± 0.261
0.0ProXaa: 0.0 ± 0.0
Gln
3.713GlnAla: 3.713 ± 0.687
0.371GlnCys: 0.371 ± 0.177
1.931GlnAsp: 1.931 ± 0.376
2.302GlnGlu: 2.302 ± 0.455
1.485GlnPhe: 1.485 ± 0.301
1.708GlnGly: 1.708 ± 0.308
0.891GlnHis: 0.891 ± 0.261
2.005GlnIle: 2.005 ± 0.316
2.896GlnLys: 2.896 ± 0.552
3.639GlnLeu: 3.639 ± 0.446
1.188GlnMet: 1.188 ± 0.255
1.559GlnAsn: 1.559 ± 0.421
1.485GlnPro: 1.485 ± 0.26
2.302GlnGln: 2.302 ± 0.692
2.599GlnArg: 2.599 ± 0.479
1.708GlnSer: 1.708 ± 0.411
2.079GlnThr: 2.079 ± 0.505
2.525GlnVal: 2.525 ± 0.406
0.743GlnTrp: 0.743 ± 0.215
1.634GlnTyr: 1.634 ± 0.365
0.0GlnXaa: 0.0 ± 0.0
Arg
4.456ArgAla: 4.456 ± 0.546
0.371ArgCys: 0.371 ± 0.198
2.451ArgAsp: 2.451 ± 0.408
3.565ArgGlu: 3.565 ± 0.582
1.857ArgPhe: 1.857 ± 0.31
3.639ArgGly: 3.639 ± 0.468
1.411ArgHis: 1.411 ± 0.264
2.525ArgIle: 2.525 ± 0.529
3.787ArgLys: 3.787 ± 0.486
4.53ArgLeu: 4.53 ± 0.608
1.782ArgMet: 1.782 ± 0.328
2.748ArgAsn: 2.748 ± 0.342
1.262ArgPro: 1.262 ± 0.302
3.416ArgGln: 3.416 ± 0.564
4.753ArgArg: 4.753 ± 0.74
2.896ArgSer: 2.896 ± 0.395
2.525ArgThr: 2.525 ± 0.443
3.193ArgVal: 3.193 ± 0.471
0.668ArgTrp: 0.668 ± 0.222
1.634ArgTyr: 1.634 ± 0.366
0.0ArgXaa: 0.0 ± 0.0
Ser
4.827SerAla: 4.827 ± 0.593
0.223SerCys: 0.223 ± 0.128
3.416SerAsp: 3.416 ± 0.426
3.045SerGlu: 3.045 ± 0.518
2.451SerPhe: 2.451 ± 0.448
6.089SerGly: 6.089 ± 0.719
1.188SerHis: 1.188 ± 0.249
3.342SerIle: 3.342 ± 0.617
3.713SerLys: 3.713 ± 0.557
4.456SerLeu: 4.456 ± 0.49
1.262SerMet: 1.262 ± 0.311
2.376SerAsn: 2.376 ± 0.445
2.525SerPro: 2.525 ± 0.454
2.302SerGln: 2.302 ± 0.569
2.822SerArg: 2.822 ± 0.444
3.267SerSer: 3.267 ± 0.973
4.01SerThr: 4.01 ± 0.509
4.604SerVal: 4.604 ± 0.461
0.52SerTrp: 0.52 ± 0.202
2.005SerTyr: 2.005 ± 0.387
0.074SerXaa: 0.074 ± 0.063
Thr
6.981ThrAla: 6.981 ± 0.953
0.668ThrCys: 0.668 ± 0.203
3.713ThrAsp: 3.713 ± 0.507
3.565ThrGlu: 3.565 ± 0.542
3.193ThrPhe: 3.193 ± 0.467
5.05ThrGly: 5.05 ± 0.827
1.188ThrHis: 1.188 ± 0.267
3.193ThrIle: 3.193 ± 0.447
3.342ThrLys: 3.342 ± 0.458
4.753ThrLeu: 4.753 ± 0.527
0.817ThrMet: 0.817 ± 0.244
1.931ThrAsn: 1.931 ± 0.351
3.713ThrPro: 3.713 ± 0.594
2.005ThrGln: 2.005 ± 0.458
2.451ThrArg: 2.451 ± 0.317
3.267ThrSer: 3.267 ± 0.462
4.01ThrThr: 4.01 ± 0.743
3.565ThrVal: 3.565 ± 0.512
1.04ThrTrp: 1.04 ± 0.297
2.005ThrTyr: 2.005 ± 0.423
0.0ThrXaa: 0.0 ± 0.0
Val
7.797ValAla: 7.797 ± 0.583
0.743ValCys: 0.743 ± 0.207
3.787ValAsp: 3.787 ± 0.444
4.604ValGlu: 4.604 ± 0.793
2.154ValPhe: 2.154 ± 0.348
4.01ValGly: 4.01 ± 0.527
0.817ValHis: 0.817 ± 0.243
4.604ValIle: 4.604 ± 0.649
3.862ValLys: 3.862 ± 0.56
5.867ValLeu: 5.867 ± 0.683
1.114ValMet: 1.114 ± 0.326
3.713ValAsn: 3.713 ± 0.607
2.228ValPro: 2.228 ± 0.544
2.97ValGln: 2.97 ± 0.482
3.787ValArg: 3.787 ± 0.436
5.05ValSer: 5.05 ± 0.422
4.975ValThr: 4.975 ± 0.686
4.53ValVal: 4.53 ± 0.719
0.891ValTrp: 0.891 ± 0.28
2.525ValTyr: 2.525 ± 0.5
0.0ValXaa: 0.0 ± 0.0
Trp
0.965TrpAla: 0.965 ± 0.387
0.074TrpCys: 0.074 ± 0.071
0.965TrpAsp: 0.965 ± 0.249
0.594TrpGlu: 0.594 ± 0.213
0.965TrpPhe: 0.965 ± 0.282
0.743TrpGly: 0.743 ± 0.263
0.371TrpHis: 0.371 ± 0.161
0.371TrpIle: 0.371 ± 0.181
0.668TrpLys: 0.668 ± 0.182
2.005TrpLeu: 2.005 ± 0.285
0.743TrpMet: 0.743 ± 0.226
0.668TrpAsn: 0.668 ± 0.265
0.446TrpPro: 0.446 ± 0.2
0.594TrpGln: 0.594 ± 0.233
0.817TrpArg: 0.817 ± 0.237
1.114TrpSer: 1.114 ± 0.417
0.743TrpThr: 0.743 ± 0.197
1.114TrpVal: 1.114 ± 0.252
0.074TrpTrp: 0.074 ± 0.07
0.446TrpTyr: 0.446 ± 0.175
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.416TyrAla: 3.416 ± 0.491
0.371TyrCys: 0.371 ± 0.15
2.525TyrAsp: 2.525 ± 0.643
2.822TyrGlu: 2.822 ± 0.478
1.931TyrPhe: 1.931 ± 0.314
2.822TyrGly: 2.822 ± 0.48
0.817TyrHis: 0.817 ± 0.239
1.782TyrIle: 1.782 ± 0.276
1.634TyrLys: 1.634 ± 0.378
2.525TyrLeu: 2.525 ± 0.408
0.371TyrMet: 0.371 ± 0.162
1.782TyrAsn: 1.782 ± 0.305
1.188TyrPro: 1.188 ± 0.429
1.411TyrGln: 1.411 ± 0.302
1.782TyrArg: 1.782 ± 0.316
2.228TyrSer: 2.228 ± 0.357
1.782TyrThr: 1.782 ± 0.392
2.005TyrVal: 2.005 ± 0.427
0.223TyrTrp: 0.223 ± 0.137
1.04TyrTyr: 1.04 ± 0.235
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.074XaaPro: 0.074 ± 0.063
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (13467 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski