Enterobacteriaceae bacterium (strain FGI 57)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; unclassified Enterobacteriaceae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4320 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0M6S4|L0M6S4_ENTBF LysM domain-containing protein OS=Enterobacteriaceae bacterium (strain FGI 57) OX=693444 GN=D782_3040 PE=4 SV=1
MM1 pKa = 7.53SDD3 pKa = 3.39EE4 pKa = 4.26VVLPLEE10 pKa = 4.42FTDD13 pKa = 4.01AAASKK18 pKa = 10.62VKK20 pKa = 10.62DD21 pKa = 4.57LIADD25 pKa = 3.62EE26 pKa = 5.74DD27 pKa = 4.1NPNMKK32 pKa = 10.11LRR34 pKa = 11.84VYY36 pKa = 9.16ITGGGCSGFQYY47 pKa = 11.03GFTFDD52 pKa = 4.33DD53 pKa = 4.35QINEE57 pKa = 3.74GDD59 pKa = 3.62MTIEE63 pKa = 3.9KK64 pKa = 10.25QGVGLVVDD72 pKa = 4.57PMSLQYY78 pKa = 11.16LVGGSVDD85 pKa = 3.52YY86 pKa = 11.39TEE88 pKa = 4.94GLEE91 pKa = 4.02GSRR94 pKa = 11.84FIVTNPNATSTCGCGSSFSII114 pKa = 5.02

Molecular weight: 12.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0M974|L0M974_ENTBF Putative nucleoside-diphosphate sugar epimerase OS=Enterobacteriaceae bacterium (strain FGI 57) OX=693444 GN=D782_3338 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4320

0

4320

1392848

29

8428

322.4

35.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.801 ± 0.04

1.057 ± 0.016

5.271 ± 0.045

5.583 ± 0.039

3.859 ± 0.031

7.556 ± 0.043

2.247 ± 0.022

5.78 ± 0.028

4.174 ± 0.035

10.759 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.774 ± 0.025

3.803 ± 0.035

4.443 ± 0.027

4.431 ± 0.029

5.506 ± 0.046

5.913 ± 0.046

5.507 ± 0.064

7.204 ± 0.041

1.534 ± 0.016

2.798 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski