Amino acid dipepetide frequency for Enterobacteriaceae bacterium (strain FGI 57)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.977AlaAla: 9.977 ± 0.116	1.116AlaCys: 1.116 ± 0.032	5.123AlaAsp: 5.123 ± 0.076	5.605AlaGlu: 5.605 ± 0.066	3.665AlaPhe: 3.665 ± 0.058	7.646AlaGly: 7.646 ± 0.115	1.872AlaHis: 1.872 ± 0.036	5.958AlaIle: 5.958 ± 0.072	3.972AlaLys: 3.972 ± 0.064	11.961AlaLeu: 11.961 ± 0.116	3.074AlaMet: 3.074 ± 0.054	3.196AlaAsn: 3.196 ± 0.06	3.669AlaPro: 3.669 ± 0.068	4.672AlaGln: 4.672 ± 0.072	5.472AlaArg: 5.472 ± 0.091	5.604AlaSer: 5.604 ± 0.072	4.98AlaThr: 4.98 ± 0.071	6.767AlaVal: 6.767 ± 0.069	1.705AlaTrp: 1.705 ± 0.04	1.976AlaTyr: 1.976 ± 0.037	0.0AlaXaa: 0.0 ± 0.0
Cys	0.958CysAla: 0.958 ± 0.031	0.164CysCys: 0.164 ± 0.011	0.617CysAsp: 0.617 ± 0.024	0.603CysGlu: 0.603 ± 0.021	0.401CysPhe: 0.401 ± 0.016	1.047CysGly: 1.047 ± 0.031	0.317CysHis: 0.317 ± 0.016	0.54CysIle: 0.54 ± 0.022	0.327CysLys: 0.327 ± 0.015	0.991CysLeu: 0.991 ± 0.032	0.239CysMet: 0.239 ± 0.012	0.33CysAsn: 0.33 ± 0.014	0.505CysPro: 0.505 ± 0.021	0.461CysGln: 0.461 ± 0.018	0.62CysArg: 0.62 ± 0.019	0.663CysSer: 0.663 ± 0.022	0.503CysThr: 0.503 ± 0.021	0.778CysVal: 0.778 ± 0.027	0.172CysTrp: 0.172 ± 0.012	0.33CysTyr: 0.33 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	5.547AspAla: 5.547 ± 0.078	0.48AspCys: 0.48 ± 0.019	3.109AspAsp: 3.109 ± 0.067	3.546AspGlu: 3.546 ± 0.054	2.017AspPhe: 2.017 ± 0.034	3.921AspGly: 3.921 ± 0.094	0.981AspHis: 0.981 ± 0.03	3.473AspIle: 3.473 ± 0.048	2.57AspLys: 2.57 ± 0.046	4.62AspLeu: 4.62 ± 0.056	1.312AspMet: 1.312 ± 0.035	2.318AspAsn: 2.318 ± 0.059	2.246AspPro: 2.246 ± 0.048	1.579AspGln: 1.579 ± 0.042	2.533AspArg: 2.533 ± 0.041	3.015AspSer: 3.015 ± 0.236	2.757AspThr: 2.757 ± 0.066	4.009AspVal: 4.009 ± 0.058	0.778AspTrp: 0.778 ± 0.026	1.903AspTyr: 1.903 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	5.232GluAla: 5.232 ± 0.074	0.478GluCys: 0.478 ± 0.023	2.312GluAsp: 2.312 ± 0.045	3.394GluGlu: 3.394 ± 0.067	1.861GluPhe: 1.861 ± 0.042	3.799GluGly: 3.799 ± 0.054	1.391GluHis: 1.391 ± 0.036	3.252GluIle: 3.252 ± 0.054	3.253GluLys: 3.253 ± 0.063	5.847GluLeu: 5.847 ± 0.078	1.823GluMet: 1.823 ± 0.039	2.441GluAsn: 2.441 ± 0.046	2.06GluPro: 2.06 ± 0.045	3.285GluGln: 3.285 ± 0.054	3.5GluArg: 3.5 ± 0.055	2.982GluSer: 2.982 ± 0.046	2.95GluThr: 2.95 ± 0.047	3.716GluVal: 3.716 ± 0.065	0.816GluTrp: 0.816 ± 0.024	1.433GluTyr: 1.433 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	3.828PheAla: 3.828 ± 0.06	0.543PheCys: 0.543 ± 0.019	2.326PheAsp: 2.326 ± 0.042	1.737PheGlu: 1.737 ± 0.031	1.727PhePhe: 1.727 ± 0.045	3.201PheGly: 3.201 ± 0.063	0.796PheHis: 0.796 ± 0.028	2.549PheIle: 2.549 ± 0.048	1.27PheLys: 1.27 ± 0.033	3.338PheLeu: 3.338 ± 0.063	0.981PheMet: 0.981 ± 0.028	1.756PheAsn: 1.756 ± 0.04	1.495PhePro: 1.495 ± 0.035	1.106PheGln: 1.106 ± 0.027	1.847PheArg: 1.847 ± 0.037	3.081PheSer: 3.081 ± 0.054	2.537PheThr: 2.537 ± 0.042	2.554PheVal: 2.554 ± 0.044	0.674PheTrp: 0.674 ± 0.024	1.246PheTyr: 1.246 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	6.324GlyAla: 6.324 ± 0.08	1.01GlyCys: 1.01 ± 0.027	3.901GlyAsp: 3.901 ± 0.084	4.699GlyGlu: 4.699 ± 0.055	3.31GlyPhe: 3.31 ± 0.051	5.673GlyGly: 5.673 ± 0.087	1.667GlyHis: 1.667 ± 0.036	4.976GlyIle: 4.976 ± 0.066	4.016GlyLys: 4.016 ± 0.058	7.608GlyLeu: 7.608 ± 0.077	2.414GlyMet: 2.414 ± 0.051	2.97GlyAsn: 2.97 ± 0.108	2.118GlyPro: 2.118 ± 0.038	2.924GlyGln: 2.924 ± 0.057	3.706GlyArg: 3.706 ± 0.062	4.339GlySer: 4.339 ± 0.09	3.965GlyThr: 3.965 ± 0.108	6.009GlyVal: 6.009 ± 0.076	1.297GlyTrp: 1.297 ± 0.034	2.634GlyTyr: 2.634 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	1.995HisAla: 1.995 ± 0.038	0.294HisCys: 0.294 ± 0.016	1.233HisAsp: 1.233 ± 0.038	1.096HisGlu: 1.096 ± 0.026	1.09HisPhe: 1.09 ± 0.027	1.725HisGly: 1.725 ± 0.04	0.771HisHis: 0.771 ± 0.03	1.349HisIle: 1.349 ± 0.032	0.732HisLys: 0.732 ± 0.027	2.225HisLeu: 2.225 ± 0.04	0.546HisMet: 0.546 ± 0.02	0.843HisAsn: 0.843 ± 0.024	1.31HisPro: 1.31 ± 0.035	1.134HisGln: 1.134 ± 0.034	1.208HisArg: 1.208 ± 0.031	1.223HisSer: 1.223 ± 0.029	1.111HisThr: 1.111 ± 0.031	1.292HisVal: 1.292 ± 0.035	0.404HisTrp: 0.404 ± 0.02	0.89HisTyr: 0.89 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	6.545IleAla: 6.545 ± 0.078	0.659IleCys: 0.659 ± 0.022	3.551IleAsp: 3.551 ± 0.06	3.211IleGlu: 3.211 ± 0.055	2.178IlePhe: 2.178 ± 0.05	4.672IleGly: 4.672 ± 0.073	1.207IleHis: 1.207 ± 0.03	3.418IleIle: 3.418 ± 0.053	2.244IleLys: 2.244 ± 0.047	4.965IleLeu: 4.965 ± 0.077	1.265IleMet: 1.265 ± 0.034	2.644IleAsn: 2.644 ± 0.054	2.812IlePro: 2.812 ± 0.048	1.796IleGln: 1.796 ± 0.039	2.878IleArg: 2.878 ± 0.045	3.832IleSer: 3.832 ± 0.056	3.619IleThr: 3.619 ± 0.069	4.041IleVal: 4.041 ± 0.061	0.69IleTrp: 0.69 ± 0.023	1.574IleTyr: 1.574 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	4.291LysAla: 4.291 ± 0.078	0.277LysCys: 0.277 ± 0.017	2.05LysAsp: 2.05 ± 0.038	2.457LysGlu: 2.457 ± 0.054	1.145LysPhe: 1.145 ± 0.032	3.013LysGly: 3.013 ± 0.052	0.867LysHis: 0.867 ± 0.023	2.333LysIle: 2.333 ± 0.042	2.15LysLys: 2.15 ± 0.053	4.293LysLeu: 4.293 ± 0.062	1.287LysMet: 1.287 ± 0.029	1.653LysAsn: 1.653 ± 0.04	2.1LysPro: 2.1 ± 0.045	1.938LysGln: 1.938 ± 0.042	2.508LysArg: 2.508 ± 0.047	2.272LysSer: 2.272 ± 0.037	2.565LysThr: 2.565 ± 0.043	2.962LysVal: 2.962 ± 0.054	0.467LysTrp: 0.467 ± 0.02	1.108LysTyr: 1.108 ± 0.034	0.0LysXaa: 0.0 ± 0.0
Leu	11.55LeuAla: 11.55 ± 0.114	1.297LeuCys: 1.297 ± 0.036	5.393LeuAsp: 5.393 ± 0.066	5.459LeuGlu: 5.459 ± 0.074	4.253LeuPhe: 4.253 ± 0.077	7.391LeuGly: 7.391 ± 0.086	2.254LeuHis: 2.254 ± 0.043	5.763LeuIle: 5.763 ± 0.084	4.507LeuLys: 4.507 ± 0.07	11.814LeuLeu: 11.814 ± 0.161	2.97LeuMet: 2.97 ± 0.054	4.343LeuAsn: 4.343 ± 0.071	5.627LeuPro: 5.627 ± 0.074	3.96LeuGln: 3.96 ± 0.066	6.093LeuArg: 6.093 ± 0.094	7.227LeuSer: 7.227 ± 0.083	6.425LeuThr: 6.425 ± 0.086	7.22LeuVal: 7.22 ± 0.084	1.468LeuTrp: 1.468 ± 0.036	2.574LeuTyr: 2.574 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	2.896MetAla: 2.896 ± 0.051	0.204MetCys: 0.204 ± 0.013	1.228MetAsp: 1.228 ± 0.031	1.243MetGlu: 1.243 ± 0.035	0.9MetPhe: 0.9 ± 0.027	1.949MetGly: 1.949 ± 0.039	0.547MetHis: 0.547 ± 0.02	1.544MetIle: 1.544 ± 0.039	1.39MetLys: 1.39 ± 0.031	3.238MetLeu: 3.238 ± 0.065	0.91MetMet: 0.91 ± 0.028	1.119MetAsn: 1.119 ± 0.028	1.376MetPro: 1.376 ± 0.033	1.226MetGln: 1.226 ± 0.032	1.471MetArg: 1.471 ± 0.029	1.915MetSer: 1.915 ± 0.039	1.809MetThr: 1.809 ± 0.032	1.981MetVal: 1.981 ± 0.042	0.269MetTrp: 0.269 ± 0.014	0.526MetTyr: 0.526 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	3.685AsnAla: 3.685 ± 0.069	0.327AsnCys: 0.327 ± 0.016	2.16AsnAsp: 2.16 ± 0.046	1.901AsnGlu: 1.901 ± 0.044	1.347AsnPhe: 1.347 ± 0.034	3.298AsnGly: 3.298 ± 0.091	0.835AsnHis: 0.835 ± 0.026	2.345AsnIle: 2.345 ± 0.051	1.487AsnLys: 1.487 ± 0.037	3.494AsnLeu: 3.494 ± 0.048	0.961AsnMet: 0.961 ± 0.031	1.646AsnAsn: 1.646 ± 0.048	2.104AsnPro: 2.104 ± 0.042	1.582AsnGln: 1.582 ± 0.036	1.973AsnArg: 1.973 ± 0.038	2.108AsnSer: 2.108 ± 0.052	2.093AsnThr: 2.093 ± 0.058	2.799AsnVal: 2.799 ± 0.062	0.605AsnTrp: 0.605 ± 0.023	1.283AsnTyr: 1.283 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	4.466ProAla: 4.466 ± 0.063	0.361ProCys: 0.361 ± 0.015	2.732ProAsp: 2.732 ± 0.046	3.328ProGlu: 3.328 ± 0.056	1.896ProPhe: 1.896 ± 0.034	3.428ProGly: 3.428 ± 0.052	1.087ProHis: 1.087 ± 0.028	1.895ProIle: 1.895 ± 0.04	1.553ProLys: 1.553 ± 0.041	5.096ProLeu: 5.096 ± 0.063	1.136ProMet: 1.136 ± 0.033	1.355ProAsn: 1.355 ± 0.03	1.615ProPro: 1.615 ± 0.036	2.255ProGln: 2.255 ± 0.048	1.98ProArg: 1.98 ± 0.042	2.199ProSer: 2.199 ± 0.042	2.15ProThr: 2.15 ± 0.046	3.928ProVal: 3.928 ± 0.058	0.788ProTrp: 0.788 ± 0.024	1.182ProTyr: 1.182 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	4.621GlnAla: 4.621 ± 0.068	0.381GlnCys: 0.381 ± 0.019	1.814GlnAsp: 1.814 ± 0.033	2.158GlnGlu: 2.158 ± 0.042	1.468GlnPhe: 1.468 ± 0.031	3.089GlnGly: 3.089 ± 0.052	1.243GlnHis: 1.243 ± 0.03	2.259GlnIle: 2.259 ± 0.039	1.836GlnLys: 1.836 ± 0.039	4.82GlnLeu: 4.82 ± 0.074	1.213GlnMet: 1.213 ± 0.029	1.437GlnAsn: 1.437 ± 0.035	2.235GlnPro: 2.235 ± 0.051	3.29GlnGln: 3.29 ± 0.07	2.954GlnArg: 2.954 ± 0.058	2.296GlnSer: 2.296 ± 0.039	2.282GlnThr: 2.282 ± 0.044	3.06GlnVal: 3.06 ± 0.051	0.663GlnTrp: 0.663 ± 0.025	1.193GlnTyr: 1.193 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	4.488ArgAla: 4.488 ± 0.064	0.556ArgCys: 0.556 ± 0.022	3.087ArgAsp: 3.087 ± 0.044	3.737ArgGlu: 3.737 ± 0.067	2.533ArgPhe: 2.533 ± 0.046	3.3ArgGly: 3.3 ± 0.058	1.504ArgHis: 1.504 ± 0.036	3.29ArgIle: 3.29 ± 0.047	2.276ArgLys: 2.276 ± 0.045	6.119ArgLeu: 6.119 ± 0.086	1.57ArgMet: 1.57 ± 0.035	1.994ArgAsn: 1.994 ± 0.038	2.122ArgPro: 2.122 ± 0.045	2.921ArgGln: 2.921 ± 0.049	3.343ArgArg: 3.343 ± 0.06	2.667ArgSer: 2.667 ± 0.046	2.484ArgThr: 2.484 ± 0.046	3.876ArgVal: 3.876 ± 0.064	0.986ArgTrp: 0.986 ± 0.026	2.21ArgTyr: 2.21 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	5.733SerAla: 5.733 ± 0.072	0.572SerCys: 0.572 ± 0.022	3.328SerAsp: 3.328 ± 0.247	3.169SerGlu: 3.169 ± 0.052	2.196SerPhe: 2.196 ± 0.044	5.426SerGly: 5.426 ± 0.092	1.414SerHis: 1.414 ± 0.033	3.011SerIle: 3.011 ± 0.047	2.076SerLys: 2.076 ± 0.043	6.707SerLeu: 6.707 ± 0.081	1.483SerMet: 1.483 ± 0.032	1.931SerAsn: 1.931 ± 0.039	2.582SerPro: 2.582 ± 0.047	2.567SerGln: 2.567 ± 0.047	3.364SerArg: 3.364 ± 0.049	3.557SerSer: 3.557 ± 0.064	3.058SerThr: 3.058 ± 0.059	4.293SerVal: 4.293 ± 0.079	1.009SerTrp: 1.009 ± 0.03	1.654SerTyr: 1.654 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	5.109ThrAla: 5.109 ± 0.095	0.534ThrCys: 0.534 ± 0.021	2.687ThrAsp: 2.687 ± 0.062	2.61ThrGlu: 2.61 ± 0.049	2.152ThrPhe: 2.152 ± 0.045	4.631ThrGly: 4.631 ± 0.098	1.273ThrHis: 1.273 ± 0.034	3.001ThrIle: 3.001 ± 0.074	1.479ThrLys: 1.479 ± 0.039	7.768ThrLeu: 7.768 ± 0.105	1.175ThrMet: 1.175 ± 0.031	1.584ThrAsn: 1.584 ± 0.042	3.336ThrPro: 3.336 ± 0.061	2.363ThrGln: 2.363 ± 0.045	3.046ThrArg: 3.046 ± 0.053	2.93ThrSer: 2.93 ± 0.051	3.137ThrThr: 3.137 ± 0.088	4.207ThrVal: 4.207 ± 0.14	0.867ThrTrp: 0.867 ± 0.027	1.179ThrTyr: 1.179 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	7.207ValAla: 7.207 ± 0.086	0.761ValCys: 0.761 ± 0.021	3.854ValAsp: 3.854 ± 0.077	4.047ValGlu: 4.047 ± 0.067	2.623ValPhe: 2.623 ± 0.047	5.108ValGly: 5.108 ± 0.069	1.258ValHis: 1.258 ± 0.029	4.65ValIle: 4.65 ± 0.072	3.154ValLys: 3.154 ± 0.053	7.391ValLeu: 7.391 ± 0.093	2.316ValMet: 2.316 ± 0.046	2.936ValAsn: 2.936 ± 0.061	3.08ValPro: 3.08 ± 0.044	2.431ValGln: 2.431 ± 0.043	3.606ValArg: 3.606 ± 0.056	4.635ValSer: 4.635 ± 0.068	4.46ValThr: 4.46 ± 0.124	5.798ValVal: 5.798 ± 0.079	0.997ValTrp: 0.997 ± 0.03	1.725ValTyr: 1.725 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	1.062TrpAla: 1.062 ± 0.029	0.184TrpCys: 0.184 ± 0.012	0.622TrpAsp: 0.622 ± 0.022	0.608TrpGlu: 0.608 ± 0.021	0.696TrpPhe: 0.696 ± 0.026	0.984TrpGly: 0.984 ± 0.028	0.462TrpHis: 0.462 ± 0.018	0.757TrpIle: 0.757 ± 0.025	0.516TrpLys: 0.516 ± 0.021	2.313TrpLeu: 2.313 ± 0.053	0.47TrpMet: 0.47 ± 0.019	0.517TrpAsn: 0.517 ± 0.025	0.664TrpPro: 0.664 ± 0.022	1.193TrpGln: 1.193 ± 0.031	1.119TrpArg: 1.119 ± 0.035	0.848TrpSer: 0.848 ± 0.035	0.638TrpThr: 0.638 ± 0.024	1.013TrpVal: 1.013 ± 0.026	0.24TrpTrp: 0.24 ± 0.015	0.429TrpTyr: 0.429 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.495TyrAla: 2.495 ± 0.039	0.37TyrCys: 0.37 ± 0.019	1.583TyrAsp: 1.583 ± 0.031	1.216TyrGlu: 1.216 ± 0.034	1.135TyrPhe: 1.135 ± 0.03	2.262TyrGly: 2.262 ± 0.048	0.723TyrHis: 0.723 ± 0.025	1.437TyrIle: 1.437 ± 0.032	0.93TyrLys: 0.93 ± 0.028	2.982TyrLeu: 2.982 ± 0.056	0.594TyrMet: 0.594 ± 0.021	1.021TyrAsn: 1.021 ± 0.033	1.371TyrPro: 1.371 ± 0.033	1.631TyrGln: 1.631 ± 0.037	1.842TyrArg: 1.842 ± 0.045	1.74TyrSer: 1.74 ± 0.041	1.546TyrThr: 1.546 ± 0.042	1.733TyrVal: 1.733 ± 0.036	0.439TyrTrp: 0.439 ± 0.017	0.928TyrTyr: 0.928 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4320 proteins (1392848 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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