Red clover powdery mildew-associated totivirus 6

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0S3Q2B4|A0A0S3Q2B4_9VIRU Capsid protein OS=Red clover powdery mildew-associated totivirus 6 OX=1714367 GN=CP PE=4 SV=1
MM1 pKa = 7.79SFYY4 pKa = 11.0TLFNTSATFPKK15 pKa = 9.33MDD17 pKa = 3.43HH18 pKa = 6.51GKK20 pKa = 10.52FGMLNRR26 pKa = 11.84LNLRR30 pKa = 11.84LAAIEE35 pKa = 4.02KK36 pKa = 10.37KK37 pKa = 10.31KK38 pKa = 10.58ISEE41 pKa = 4.07AGYY44 pKa = 10.44DD45 pKa = 3.65GTRR48 pKa = 11.84EE49 pKa = 3.87EE50 pKa = 5.45AIAAPSGKK58 pKa = 8.73TSTLANEE65 pKa = 4.31ITDD68 pKa = 3.64EE69 pKa = 4.37LGFEE73 pKa = 4.12IDD75 pKa = 4.66HH76 pKa = 6.81RR77 pKa = 11.84APTNTLAEE85 pKa = 4.08GTIYY89 pKa = 10.58GRR91 pKa = 11.84NGTWRR96 pKa = 11.84ALGRR100 pKa = 11.84EE101 pKa = 4.04NTTSGLNPLSLNEE114 pKa = 4.58SGAIDD119 pKa = 4.13HH120 pKa = 6.58KK121 pKa = 10.12WLRR124 pKa = 11.84AQIRR128 pKa = 11.84EE129 pKa = 4.18LNPYY133 pKa = 10.02DD134 pKa = 3.9EE135 pKa = 4.54NRR137 pKa = 11.84EE138 pKa = 3.78ARR140 pKa = 11.84LNSFTTSWLTSVCYY154 pKa = 10.77DD155 pKa = 3.2NATALLLALGQRR167 pKa = 11.84LVIARR172 pKa = 11.84AAEE175 pKa = 3.84LAGTKK180 pKa = 8.08VVRR183 pKa = 11.84LVILEE188 pKa = 4.28SEE190 pKa = 4.49YY191 pKa = 11.02KK192 pKa = 10.86ANGLATLQNMVNHH205 pKa = 6.79ISEE208 pKa = 4.48KK209 pKa = 10.88LPTLFNGGIDD219 pKa = 3.61IANHH223 pKa = 7.07DD224 pKa = 4.65DD225 pKa = 5.26LISHH229 pKa = 7.11LAKK232 pKa = 10.92LLITKK237 pKa = 8.3FKK239 pKa = 10.51KK240 pKa = 10.48LPPLTEE246 pKa = 4.48AEE248 pKa = 4.49PKK250 pKa = 9.43RR251 pKa = 11.84AAYY254 pKa = 10.03EE255 pKa = 3.71DD256 pKa = 3.54VRR258 pKa = 11.84YY259 pKa = 9.63FEE261 pKa = 5.94CNEE264 pKa = 3.59FDD266 pKa = 4.61DD267 pKa = 4.21GHH269 pKa = 6.44SRR271 pKa = 11.84SGPTFGDD278 pKa = 3.32VYY280 pKa = 11.02GFSRR284 pKa = 11.84NRR286 pKa = 11.84ALVPLKK292 pKa = 10.92AFFAQYY298 pKa = 10.89GDD300 pKa = 3.34EE301 pKa = 4.8SVFKK305 pKa = 11.01DD306 pKa = 3.3EE307 pKa = 5.95GYY309 pKa = 11.2LSVQDD314 pKa = 4.43NEE316 pKa = 4.33HH317 pKa = 6.04EE318 pKa = 4.18QLFFNARR325 pKa = 11.84GHH327 pKa = 6.01VNLGTLTRR335 pKa = 11.84SEE337 pKa = 4.26IVFLDD342 pKa = 3.4SVIRR346 pKa = 11.84GNTRR350 pKa = 11.84QSPLMVDD357 pKa = 3.16QTFSLLPEE365 pKa = 4.76GSYY368 pKa = 11.4LMAYY372 pKa = 10.33NSTTTRR378 pKa = 11.84IEE380 pKa = 4.04DD381 pKa = 3.69EE382 pKa = 4.28AVVTSTLIRR391 pKa = 11.84SVIRR395 pKa = 11.84KK396 pKa = 8.45LVNNHH401 pKa = 5.12NWYY404 pKa = 10.35EE405 pKa = 4.1DD406 pKa = 3.17ARR408 pKa = 11.84TANLLLQQWSAQPATEE424 pKa = 4.17SVEE427 pKa = 4.42SHH429 pKa = 5.36WWPSLEE435 pKa = 3.93RR436 pKa = 11.84DD437 pKa = 3.61LRR439 pKa = 11.84VPTLGLGRR447 pKa = 11.84AAIPGLIGKK456 pKa = 9.8YY457 pKa = 9.48GVNVSKK463 pKa = 10.68QALIEE468 pKa = 4.34GQDD471 pKa = 3.66LYY473 pKa = 11.76GDD475 pKa = 4.46DD476 pKa = 4.08DD477 pKa = 5.02SKK479 pKa = 11.19FVKK482 pKa = 10.57GLIFNTLWYY491 pKa = 8.16WGEE494 pKa = 3.97YY495 pKa = 7.59LTIMNSSTMKK505 pKa = 10.51QLADD509 pKa = 3.38KK510 pKa = 10.91LRR512 pKa = 11.84FGGKK516 pKa = 8.17EE517 pKa = 3.6WLSEE521 pKa = 3.89QDD523 pKa = 2.76RR524 pKa = 11.84SYY526 pKa = 12.24AMISAISGMAVDD538 pKa = 5.54APASNISSTSIVGGIEE554 pKa = 3.72SQLRR558 pKa = 11.84NRR560 pKa = 11.84VSFGYY565 pKa = 9.75IDD567 pKa = 3.3TSSIVRR573 pKa = 11.84HH574 pKa = 6.31GYY576 pKa = 9.32SVSGSSLTYY585 pKa = 10.56NRR587 pKa = 11.84LVQPGSTCLIVGLNGTLIRR606 pKa = 11.84STPYY610 pKa = 10.66SSVFCLGAASSVDD623 pKa = 3.74VKK625 pKa = 10.57WRR627 pKa = 11.84TTMAYY632 pKa = 8.93TYY634 pKa = 11.17NDD636 pKa = 2.94MWALGVVARR645 pKa = 11.84WQGYY649 pKa = 9.51DD650 pKa = 2.78VDD652 pKa = 4.76YY653 pKa = 11.04RR654 pKa = 11.84LPDD657 pKa = 3.19QSEE660 pKa = 4.28TSNRR664 pKa = 11.84LHH666 pKa = 6.92AANDD670 pKa = 3.46SSVAYY675 pKa = 9.02PPLVPTLAQRR685 pKa = 11.84RR686 pKa = 11.84GFYY689 pKa = 10.05PIVDD693 pKa = 3.66IKK695 pKa = 10.51PRR697 pKa = 11.84KK698 pKa = 9.11RR699 pKa = 11.84CFGTDD704 pKa = 2.89TASLHH709 pKa = 5.41TYY711 pKa = 8.71KK712 pKa = 9.73RR713 pKa = 11.84TYY715 pKa = 9.33VWTRR719 pKa = 11.84DD720 pKa = 3.23TPTALEE726 pKa = 3.88QCRR729 pKa = 11.84LDD731 pKa = 3.42ARR733 pKa = 11.84LVGSEE738 pKa = 4.35PPSAAHH744 pKa = 6.93ALMVDD749 pKa = 3.52STTIATTSKK758 pKa = 10.4KK759 pKa = 10.36YY760 pKa = 10.69YY761 pKa = 9.63IRR763 pKa = 11.84SDD765 pKa = 3.4YY766 pKa = 10.47DD767 pKa = 3.47LSMSGFHH774 pKa = 4.98VTEE777 pKa = 4.34LATAITLPDD786 pKa = 3.17ATVALEE792 pKa = 4.05STRR795 pKa = 11.84LEE797 pKa = 4.65SIDD800 pKa = 4.46DD801 pKa = 4.02LPVDD805 pKa = 3.33QHH807 pKa = 6.52EE808 pKa = 4.2NN809 pKa = 3.19

Molecular weight:
90.03 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0S3Q2B4|A0A0S3Q2B4_9VIRU Capsid protein OS=Red clover powdery mildew-associated totivirus 6 OX=1714367 GN=CP PE=4 SV=1
MM1 pKa = 7.87DD2 pKa = 5.66GIARR6 pKa = 11.84STTFEE11 pKa = 3.87RR12 pKa = 11.84ASYY15 pKa = 10.36SLVEE19 pKa = 4.95IIPGIDD25 pKa = 2.75IRR27 pKa = 11.84GQFFCPTYY35 pKa = 10.23DD36 pKa = 3.02GAMRR40 pKa = 11.84VCSVYY45 pKa = 10.7YY46 pKa = 10.18RR47 pKa = 11.84DD48 pKa = 3.51LHH50 pKa = 5.67MSAMYY55 pKa = 10.22LSHH58 pKa = 7.56VEE60 pKa = 4.2SLPALLPAARR70 pKa = 11.84VAVSRR75 pKa = 11.84TQFGYY80 pKa = 10.32DD81 pKa = 3.99LCPLGCVSQKK91 pKa = 9.6EE92 pKa = 4.15HH93 pKa = 6.99LAFLTNYY100 pKa = 9.91SSEE103 pKa = 3.83DD104 pKa = 3.73RR105 pKa = 11.84YY106 pKa = 9.2TSRR109 pKa = 11.84HH110 pKa = 4.71FEE112 pKa = 4.0KK113 pKa = 10.76LSAVLAGRR121 pKa = 11.84IEE123 pKa = 4.26LPHH126 pKa = 6.48EE127 pKa = 4.51KK128 pKa = 10.51ASATHH133 pKa = 6.07LRR135 pKa = 11.84HH136 pKa = 6.51IVLADD141 pKa = 3.61IAAVMAEE148 pKa = 4.18RR149 pKa = 11.84QQGVPDD155 pKa = 4.78LGNVAYY161 pKa = 10.58LLEE164 pKa = 4.13NAIRR168 pKa = 11.84DD169 pKa = 3.53GWATEE174 pKa = 4.07TFVVGMILWVLALRR188 pKa = 11.84QPIRR192 pKa = 11.84GWVLGSKK199 pKa = 8.17WWLQAHH205 pKa = 6.72ANMKK209 pKa = 9.73EE210 pKa = 3.73YY211 pKa = 10.71HH212 pKa = 6.85DD213 pKa = 4.5FIKK216 pKa = 10.17TKK218 pKa = 10.66LSLRR222 pKa = 11.84AKK224 pKa = 10.22ALQNLVGVDD233 pKa = 3.93LSQLFEE239 pKa = 4.37VEE241 pKa = 4.22VLVNRR246 pKa = 11.84GMGQVDD252 pKa = 3.59WKK254 pKa = 11.08AEE256 pKa = 3.78RR257 pKa = 11.84LHH259 pKa = 6.5RR260 pKa = 11.84VRR262 pKa = 11.84PSLATFGVDD271 pKa = 4.25AIRR274 pKa = 11.84AEE276 pKa = 4.12ACKK279 pKa = 10.54LFTKK283 pKa = 10.48SKK285 pKa = 10.15QLGGKK290 pKa = 9.0LRR292 pKa = 11.84PRR294 pKa = 11.84DD295 pKa = 3.3WGSYY299 pKa = 7.67WDD301 pKa = 4.48TRR303 pKa = 11.84WMWAPTGAFFSQHH316 pKa = 6.66PEE318 pKa = 3.7DD319 pKa = 4.01KK320 pKa = 10.77AYY322 pKa = 10.8LSKK325 pKa = 11.11DD326 pKa = 3.22FSSRR330 pKa = 11.84SKK332 pKa = 10.66FYY334 pKa = 10.92ALSSMPDD341 pKa = 3.12YY342 pKa = 11.11GLEE345 pKa = 3.93HH346 pKa = 6.52FLGRR350 pKa = 11.84RR351 pKa = 11.84CEE353 pKa = 4.11IQAKK357 pKa = 10.09ASQKK361 pKa = 9.77YY362 pKa = 7.1EE363 pKa = 3.8WGKK366 pKa = 9.04MRR368 pKa = 11.84AIYY371 pKa = 8.53GTDD374 pKa = 2.86VTSFVLTNFCMGDD387 pKa = 3.43CEE389 pKa = 4.61EE390 pKa = 4.26FLEE393 pKa = 4.75PVFPLKK399 pKa = 10.4EE400 pKa = 3.89AATLSHH406 pKa = 5.73VKK408 pKa = 10.56ARR410 pKa = 11.84VKK412 pKa = 10.59DD413 pKa = 3.68VLNNGVPYY421 pKa = 10.83CFDD424 pKa = 3.78FEE426 pKa = 5.21DD427 pKa = 5.0FNSQHH432 pKa = 6.68SGDD435 pKa = 3.62AMEE438 pKa = 5.17AVLLAFGEE446 pKa = 4.57VCEE449 pKa = 5.71DD450 pKa = 3.54MMSHH454 pKa = 5.91EE455 pKa = 3.96QRR457 pKa = 11.84AAFEE461 pKa = 4.42WTCGSIGKK469 pKa = 7.52QTIHH473 pKa = 6.9SPDD476 pKa = 3.28GKK478 pKa = 10.51DD479 pKa = 3.11YY480 pKa = 7.48TTKK483 pKa = 9.39HH484 pKa = 5.52TLLSGWRR491 pKa = 11.84LTTFMNTVLNYY502 pKa = 9.83IYY504 pKa = 10.87VQLCSEE510 pKa = 4.67GEE512 pKa = 4.01LAATIHH518 pKa = 6.24TGDD521 pKa = 4.07DD522 pKa = 3.12VLAAVEE528 pKa = 4.02NLGAVATLQRR538 pKa = 11.84NAIEE542 pKa = 4.24HH543 pKa = 5.5NVRR546 pKa = 11.84FQRR549 pKa = 11.84SKK551 pKa = 11.26CFLGGLAEE559 pKa = 4.4FVRR562 pKa = 11.84VDD564 pKa = 3.49HH565 pKa = 7.0LSGTGAQYY573 pKa = 10.98LARR576 pKa = 11.84ACSSFVHH583 pKa = 6.72GPMDD587 pKa = 3.26MSIPFDD593 pKa = 3.71PLAQVEE599 pKa = 4.27AMHH602 pKa = 6.43TRR604 pKa = 11.84TLEE607 pKa = 4.17CKK609 pKa = 9.96EE610 pKa = 4.0RR611 pKa = 11.84GADD614 pKa = 3.67VEE616 pKa = 4.69LMNALEE622 pKa = 4.29RR623 pKa = 11.84RR624 pKa = 11.84AISYY628 pKa = 8.52VLQVTDD634 pKa = 4.21CEE636 pKa = 4.36NVNVDD641 pKa = 5.09DD642 pKa = 4.89ILNTHH647 pKa = 5.85VAYY650 pKa = 10.41GGMSRR655 pKa = 11.84EE656 pKa = 3.86IDD658 pKa = 3.2EE659 pKa = 4.45GAVANKK665 pKa = 9.44IEE667 pKa = 4.04RR668 pKa = 11.84RR669 pKa = 11.84AVVDD673 pKa = 4.43LLEE676 pKa = 4.7EE677 pKa = 4.39PPGPEE682 pKa = 3.46EE683 pKa = 4.98DD684 pKa = 3.88GSNKK688 pKa = 9.54ILPGCKK694 pKa = 9.31AYY696 pKa = 10.3ARR698 pKa = 11.84HH699 pKa = 5.76VARR702 pKa = 11.84TYY704 pKa = 11.02NLQEE708 pKa = 4.28HH709 pKa = 5.86EE710 pKa = 4.24LKK712 pKa = 10.21IRR714 pKa = 11.84RR715 pKa = 11.84RR716 pKa = 11.84VQQALDD722 pKa = 3.4ASVTKK727 pKa = 9.43TKK729 pKa = 10.74YY730 pKa = 10.38AIRR733 pKa = 11.84VVKK736 pKa = 10.17NVSSISQSIARR747 pKa = 11.84ISQFKK752 pKa = 10.39KK753 pKa = 9.79FANIKK758 pKa = 10.45LGMKK762 pKa = 10.41ANLAKK767 pKa = 10.8AFGVPLTTGDD777 pKa = 3.26STQDD781 pKa = 2.87VKK783 pKa = 10.57TAMLISSEE791 pKa = 4.2DD792 pKa = 3.15QLAALRR798 pKa = 11.84LWFF801 pKa = 4.49

Molecular weight:
89.85 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1610

801

809

805.0

89.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.068 ± 0.562

1.429 ± 0.49

5.59 ± 0.157

6.025 ± 0.242

3.851 ± 0.367

6.522 ± 0.109

2.609 ± 0.362

4.534 ± 0.293

4.41 ± 0.413

10.186 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.298 ± 0.405

4.037 ± 0.648

3.478 ± 0.252

3.23 ± 0.364

6.335 ± 0.022

7.764 ± 0.723

6.584 ± 1.036

6.584 ± 0.641

1.739 ± 0.006

3.727 ± 0.34

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski