Red clover powdery mildew-associated totivirus 6
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S3Q2B4|A0A0S3Q2B4_9VIRU Capsid protein OS=Red clover powdery mildew-associated totivirus 6 OX=1714367 GN=CP PE=4 SV=1
MM1 pKa = 7.79 SFYY4 pKa = 11.0 TLFNTSATFPKK15 pKa = 9.33 MDD17 pKa = 3.43 HH18 pKa = 6.51 GKK20 pKa = 10.52 FGMLNRR26 pKa = 11.84 LNLRR30 pKa = 11.84 LAAIEE35 pKa = 4.02 KK36 pKa = 10.37 KK37 pKa = 10.31 KK38 pKa = 10.58 ISEE41 pKa = 4.07 AGYY44 pKa = 10.44 DD45 pKa = 3.65 GTRR48 pKa = 11.84 EE49 pKa = 3.87 EE50 pKa = 5.45 AIAAPSGKK58 pKa = 8.73 TSTLANEE65 pKa = 4.31 ITDD68 pKa = 3.64 EE69 pKa = 4.37 LGFEE73 pKa = 4.12 IDD75 pKa = 4.66 HH76 pKa = 6.81 RR77 pKa = 11.84 APTNTLAEE85 pKa = 4.08 GTIYY89 pKa = 10.58 GRR91 pKa = 11.84 NGTWRR96 pKa = 11.84 ALGRR100 pKa = 11.84 EE101 pKa = 4.04 NTTSGLNPLSLNEE114 pKa = 4.58 SGAIDD119 pKa = 4.13 HH120 pKa = 6.58 KK121 pKa = 10.12 WLRR124 pKa = 11.84 AQIRR128 pKa = 11.84 EE129 pKa = 4.18 LNPYY133 pKa = 10.02 DD134 pKa = 3.9 EE135 pKa = 4.54 NRR137 pKa = 11.84 EE138 pKa = 3.78 ARR140 pKa = 11.84 LNSFTTSWLTSVCYY154 pKa = 10.77 DD155 pKa = 3.2 NATALLLALGQRR167 pKa = 11.84 LVIARR172 pKa = 11.84 AAEE175 pKa = 3.84 LAGTKK180 pKa = 8.08 VVRR183 pKa = 11.84 LVILEE188 pKa = 4.28 SEE190 pKa = 4.49 YY191 pKa = 11.02 KK192 pKa = 10.86 ANGLATLQNMVNHH205 pKa = 6.79 ISEE208 pKa = 4.48 KK209 pKa = 10.88 LPTLFNGGIDD219 pKa = 3.61 IANHH223 pKa = 7.07 DD224 pKa = 4.65 DD225 pKa = 5.26 LISHH229 pKa = 7.11 LAKK232 pKa = 10.92 LLITKK237 pKa = 8.3 FKK239 pKa = 10.51 KK240 pKa = 10.48 LPPLTEE246 pKa = 4.48 AEE248 pKa = 4.49 PKK250 pKa = 9.43 RR251 pKa = 11.84 AAYY254 pKa = 10.03 EE255 pKa = 3.71 DD256 pKa = 3.54 VRR258 pKa = 11.84 YY259 pKa = 9.63 FEE261 pKa = 5.94 CNEE264 pKa = 3.59 FDD266 pKa = 4.61 DD267 pKa = 4.21 GHH269 pKa = 6.44 SRR271 pKa = 11.84 SGPTFGDD278 pKa = 3.32 VYY280 pKa = 11.02 GFSRR284 pKa = 11.84 NRR286 pKa = 11.84 ALVPLKK292 pKa = 10.92 AFFAQYY298 pKa = 10.89 GDD300 pKa = 3.34 EE301 pKa = 4.8 SVFKK305 pKa = 11.01 DD306 pKa = 3.3 EE307 pKa = 5.95 GYY309 pKa = 11.2 LSVQDD314 pKa = 4.43 NEE316 pKa = 4.33 HH317 pKa = 6.04 EE318 pKa = 4.18 QLFFNARR325 pKa = 11.84 GHH327 pKa = 6.01 VNLGTLTRR335 pKa = 11.84 SEE337 pKa = 4.26 IVFLDD342 pKa = 3.4 SVIRR346 pKa = 11.84 GNTRR350 pKa = 11.84 QSPLMVDD357 pKa = 3.16 QTFSLLPEE365 pKa = 4.76 GSYY368 pKa = 11.4 LMAYY372 pKa = 10.33 NSTTTRR378 pKa = 11.84 IEE380 pKa = 4.04 DD381 pKa = 3.69 EE382 pKa = 4.28 AVVTSTLIRR391 pKa = 11.84 SVIRR395 pKa = 11.84 KK396 pKa = 8.45 LVNNHH401 pKa = 5.12 NWYY404 pKa = 10.35 EE405 pKa = 4.1 DD406 pKa = 3.17 ARR408 pKa = 11.84 TANLLLQQWSAQPATEE424 pKa = 4.17 SVEE427 pKa = 4.42 SHH429 pKa = 5.36 WWPSLEE435 pKa = 3.93 RR436 pKa = 11.84 DD437 pKa = 3.61 LRR439 pKa = 11.84 VPTLGLGRR447 pKa = 11.84 AAIPGLIGKK456 pKa = 9.8 YY457 pKa = 9.48 GVNVSKK463 pKa = 10.68 QALIEE468 pKa = 4.34 GQDD471 pKa = 3.66 LYY473 pKa = 11.76 GDD475 pKa = 4.46 DD476 pKa = 4.08 DD477 pKa = 5.02 SKK479 pKa = 11.19 FVKK482 pKa = 10.57 GLIFNTLWYY491 pKa = 8.16 WGEE494 pKa = 3.97 YY495 pKa = 7.59 LTIMNSSTMKK505 pKa = 10.51 QLADD509 pKa = 3.38 KK510 pKa = 10.91 LRR512 pKa = 11.84 FGGKK516 pKa = 8.17 EE517 pKa = 3.6 WLSEE521 pKa = 3.89 QDD523 pKa = 2.76 RR524 pKa = 11.84 SYY526 pKa = 12.24 AMISAISGMAVDD538 pKa = 5.54 APASNISSTSIVGGIEE554 pKa = 3.72 SQLRR558 pKa = 11.84 NRR560 pKa = 11.84 VSFGYY565 pKa = 9.75 IDD567 pKa = 3.3 TSSIVRR573 pKa = 11.84 HH574 pKa = 6.31 GYY576 pKa = 9.32 SVSGSSLTYY585 pKa = 10.56 NRR587 pKa = 11.84 LVQPGSTCLIVGLNGTLIRR606 pKa = 11.84 STPYY610 pKa = 10.66 SSVFCLGAASSVDD623 pKa = 3.74 VKK625 pKa = 10.57 WRR627 pKa = 11.84 TTMAYY632 pKa = 8.93 TYY634 pKa = 11.17 NDD636 pKa = 2.94 MWALGVVARR645 pKa = 11.84 WQGYY649 pKa = 9.51 DD650 pKa = 2.78 VDD652 pKa = 4.76 YY653 pKa = 11.04 RR654 pKa = 11.84 LPDD657 pKa = 3.19 QSEE660 pKa = 4.28 TSNRR664 pKa = 11.84 LHH666 pKa = 6.92 AANDD670 pKa = 3.46 SSVAYY675 pKa = 9.02 PPLVPTLAQRR685 pKa = 11.84 RR686 pKa = 11.84 GFYY689 pKa = 10.05 PIVDD693 pKa = 3.66 IKK695 pKa = 10.51 PRR697 pKa = 11.84 KK698 pKa = 9.11 RR699 pKa = 11.84 CFGTDD704 pKa = 2.89 TASLHH709 pKa = 5.41 TYY711 pKa = 8.71 KK712 pKa = 9.73 RR713 pKa = 11.84 TYY715 pKa = 9.33 VWTRR719 pKa = 11.84 DD720 pKa = 3.23 TPTALEE726 pKa = 3.88 QCRR729 pKa = 11.84 LDD731 pKa = 3.42 ARR733 pKa = 11.84 LVGSEE738 pKa = 4.35 PPSAAHH744 pKa = 6.93 ALMVDD749 pKa = 3.52 STTIATTSKK758 pKa = 10.4 KK759 pKa = 10.36 YY760 pKa = 10.69 YY761 pKa = 9.63 IRR763 pKa = 11.84 SDD765 pKa = 3.4 YY766 pKa = 10.47 DD767 pKa = 3.47 LSMSGFHH774 pKa = 4.98 VTEE777 pKa = 4.34 LATAITLPDD786 pKa = 3.17 ATVALEE792 pKa = 4.05 STRR795 pKa = 11.84 LEE797 pKa = 4.65 SIDD800 pKa = 4.46 DD801 pKa = 4.02 LPVDD805 pKa = 3.33 QHH807 pKa = 6.52 EE808 pKa = 4.2 NN809 pKa = 3.19
Molecular weight: 90.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.673
IPC2_protein 5.639
IPC_protein 5.69
Toseland 6.071
ProMoST 5.995
Dawson 5.906
Bjellqvist 5.931
Wikipedia 5.893
Rodwell 5.881
Grimsley 6.237
Solomon 5.906
Lehninger 5.893
Nozaki 6.148
DTASelect 6.338
Thurlkill 6.326
EMBOSS 6.287
Sillero 6.249
Patrickios 4.126
IPC_peptide 5.919
IPC2_peptide 6.249
IPC2.peptide.svr19 6.187
Protein with the highest isoelectric point:
>tr|A0A0S3Q2B4|A0A0S3Q2B4_9VIRU Capsid protein OS=Red clover powdery mildew-associated totivirus 6 OX=1714367 GN=CP PE=4 SV=1
MM1 pKa = 7.87 DD2 pKa = 5.66 GIARR6 pKa = 11.84 STTFEE11 pKa = 3.87 RR12 pKa = 11.84 ASYY15 pKa = 10.36 SLVEE19 pKa = 4.95 IIPGIDD25 pKa = 2.75 IRR27 pKa = 11.84 GQFFCPTYY35 pKa = 10.23 DD36 pKa = 3.02 GAMRR40 pKa = 11.84 VCSVYY45 pKa = 10.7 YY46 pKa = 10.18 RR47 pKa = 11.84 DD48 pKa = 3.51 LHH50 pKa = 5.67 MSAMYY55 pKa = 10.22 LSHH58 pKa = 7.56 VEE60 pKa = 4.2 SLPALLPAARR70 pKa = 11.84 VAVSRR75 pKa = 11.84 TQFGYY80 pKa = 10.32 DD81 pKa = 3.99 LCPLGCVSQKK91 pKa = 9.6 EE92 pKa = 4.15 HH93 pKa = 6.99 LAFLTNYY100 pKa = 9.91 SSEE103 pKa = 3.83 DD104 pKa = 3.73 RR105 pKa = 11.84 YY106 pKa = 9.2 TSRR109 pKa = 11.84 HH110 pKa = 4.71 FEE112 pKa = 4.0 KK113 pKa = 10.76 LSAVLAGRR121 pKa = 11.84 IEE123 pKa = 4.26 LPHH126 pKa = 6.48 EE127 pKa = 4.51 KK128 pKa = 10.51 ASATHH133 pKa = 6.07 LRR135 pKa = 11.84 HH136 pKa = 6.51 IVLADD141 pKa = 3.61 IAAVMAEE148 pKa = 4.18 RR149 pKa = 11.84 QQGVPDD155 pKa = 4.78 LGNVAYY161 pKa = 10.58 LLEE164 pKa = 4.13 NAIRR168 pKa = 11.84 DD169 pKa = 3.53 GWATEE174 pKa = 4.07 TFVVGMILWVLALRR188 pKa = 11.84 QPIRR192 pKa = 11.84 GWVLGSKK199 pKa = 8.17 WWLQAHH205 pKa = 6.72 ANMKK209 pKa = 9.73 EE210 pKa = 3.73 YY211 pKa = 10.71 HH212 pKa = 6.85 DD213 pKa = 4.5 FIKK216 pKa = 10.17 TKK218 pKa = 10.66 LSLRR222 pKa = 11.84 AKK224 pKa = 10.22 ALQNLVGVDD233 pKa = 3.93 LSQLFEE239 pKa = 4.37 VEE241 pKa = 4.22 VLVNRR246 pKa = 11.84 GMGQVDD252 pKa = 3.59 WKK254 pKa = 11.08 AEE256 pKa = 3.78 RR257 pKa = 11.84 LHH259 pKa = 6.5 RR260 pKa = 11.84 VRR262 pKa = 11.84 PSLATFGVDD271 pKa = 4.25 AIRR274 pKa = 11.84 AEE276 pKa = 4.12 ACKK279 pKa = 10.54 LFTKK283 pKa = 10.48 SKK285 pKa = 10.15 QLGGKK290 pKa = 9.0 LRR292 pKa = 11.84 PRR294 pKa = 11.84 DD295 pKa = 3.3 WGSYY299 pKa = 7.67 WDD301 pKa = 4.48 TRR303 pKa = 11.84 WMWAPTGAFFSQHH316 pKa = 6.66 PEE318 pKa = 3.7 DD319 pKa = 4.01 KK320 pKa = 10.77 AYY322 pKa = 10.8 LSKK325 pKa = 11.11 DD326 pKa = 3.22 FSSRR330 pKa = 11.84 SKK332 pKa = 10.66 FYY334 pKa = 10.92 ALSSMPDD341 pKa = 3.12 YY342 pKa = 11.11 GLEE345 pKa = 3.93 HH346 pKa = 6.52 FLGRR350 pKa = 11.84 RR351 pKa = 11.84 CEE353 pKa = 4.11 IQAKK357 pKa = 10.09 ASQKK361 pKa = 9.77 YY362 pKa = 7.1 EE363 pKa = 3.8 WGKK366 pKa = 9.04 MRR368 pKa = 11.84 AIYY371 pKa = 8.53 GTDD374 pKa = 2.86 VTSFVLTNFCMGDD387 pKa = 3.43 CEE389 pKa = 4.61 EE390 pKa = 4.26 FLEE393 pKa = 4.75 PVFPLKK399 pKa = 10.4 EE400 pKa = 3.89 AATLSHH406 pKa = 5.73 VKK408 pKa = 10.56 ARR410 pKa = 11.84 VKK412 pKa = 10.59 DD413 pKa = 3.68 VLNNGVPYY421 pKa = 10.83 CFDD424 pKa = 3.78 FEE426 pKa = 5.21 DD427 pKa = 5.0 FNSQHH432 pKa = 6.68 SGDD435 pKa = 3.62 AMEE438 pKa = 5.17 AVLLAFGEE446 pKa = 4.57 VCEE449 pKa = 5.71 DD450 pKa = 3.54 MMSHH454 pKa = 5.91 EE455 pKa = 3.96 QRR457 pKa = 11.84 AAFEE461 pKa = 4.42 WTCGSIGKK469 pKa = 7.52 QTIHH473 pKa = 6.9 SPDD476 pKa = 3.28 GKK478 pKa = 10.51 DD479 pKa = 3.11 YY480 pKa = 7.48 TTKK483 pKa = 9.39 HH484 pKa = 5.52 TLLSGWRR491 pKa = 11.84 LTTFMNTVLNYY502 pKa = 9.83 IYY504 pKa = 10.87 VQLCSEE510 pKa = 4.67 GEE512 pKa = 4.01 LAATIHH518 pKa = 6.24 TGDD521 pKa = 4.07 DD522 pKa = 3.12 VLAAVEE528 pKa = 4.02 NLGAVATLQRR538 pKa = 11.84 NAIEE542 pKa = 4.24 HH543 pKa = 5.5 NVRR546 pKa = 11.84 FQRR549 pKa = 11.84 SKK551 pKa = 11.26 CFLGGLAEE559 pKa = 4.4 FVRR562 pKa = 11.84 VDD564 pKa = 3.49 HH565 pKa = 7.0 LSGTGAQYY573 pKa = 10.98 LARR576 pKa = 11.84 ACSSFVHH583 pKa = 6.72 GPMDD587 pKa = 3.26 MSIPFDD593 pKa = 3.71 PLAQVEE599 pKa = 4.27 AMHH602 pKa = 6.43 TRR604 pKa = 11.84 TLEE607 pKa = 4.17 CKK609 pKa = 9.96 EE610 pKa = 4.0 RR611 pKa = 11.84 GADD614 pKa = 3.67 VEE616 pKa = 4.69 LMNALEE622 pKa = 4.29 RR623 pKa = 11.84 RR624 pKa = 11.84 AISYY628 pKa = 8.52 VLQVTDD634 pKa = 4.21 CEE636 pKa = 4.36 NVNVDD641 pKa = 5.09 DD642 pKa = 4.89 ILNTHH647 pKa = 5.85 VAYY650 pKa = 10.41 GGMSRR655 pKa = 11.84 EE656 pKa = 3.86 IDD658 pKa = 3.2 EE659 pKa = 4.45 GAVANKK665 pKa = 9.44 IEE667 pKa = 4.04 RR668 pKa = 11.84 RR669 pKa = 11.84 AVVDD673 pKa = 4.43 LLEE676 pKa = 4.7 EE677 pKa = 4.39 PPGPEE682 pKa = 3.46 EE683 pKa = 4.98 DD684 pKa = 3.88 GSNKK688 pKa = 9.54 ILPGCKK694 pKa = 9.31 AYY696 pKa = 10.3 ARR698 pKa = 11.84 HH699 pKa = 5.76 VARR702 pKa = 11.84 TYY704 pKa = 11.02 NLQEE708 pKa = 4.28 HH709 pKa = 5.86 EE710 pKa = 4.24 LKK712 pKa = 10.21 IRR714 pKa = 11.84 RR715 pKa = 11.84 RR716 pKa = 11.84 VQQALDD722 pKa = 3.4 ASVTKK727 pKa = 9.43 TKK729 pKa = 10.74 YY730 pKa = 10.38 AIRR733 pKa = 11.84 VVKK736 pKa = 10.17 NVSSISQSIARR747 pKa = 11.84 ISQFKK752 pKa = 10.39 KK753 pKa = 9.79 FANIKK758 pKa = 10.45 LGMKK762 pKa = 10.41 ANLAKK767 pKa = 10.8 AFGVPLTTGDD777 pKa = 3.26 STQDD781 pKa = 2.87 VKK783 pKa = 10.57 TAMLISSEE791 pKa = 4.2 DD792 pKa = 3.15 QLAALRR798 pKa = 11.84 LWFF801 pKa = 4.49
Molecular weight: 89.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.334
IPC2_protein 6.338
IPC_protein 6.415
Toseland 6.605
ProMoST 6.825
Dawson 6.81
Bjellqvist 6.81
Wikipedia 6.81
Rodwell 6.81
Grimsley 6.722
Solomon 6.81
Lehninger 6.81
Nozaki 7.117
DTASelect 7.22
Thurlkill 7.263
EMBOSS 7.234
Sillero 7.22
Patrickios 4.228
IPC_peptide 6.825
IPC2_peptide 6.985
IPC2.peptide.svr19 6.857
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1610
801
809
805.0
89.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.068 ± 0.562
1.429 ± 0.49
5.59 ± 0.157
6.025 ± 0.242
3.851 ± 0.367
6.522 ± 0.109
2.609 ± 0.362
4.534 ± 0.293
4.41 ± 0.413
10.186 ± 0.317
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.298 ± 0.405
4.037 ± 0.648
3.478 ± 0.252
3.23 ± 0.364
6.335 ± 0.022
7.764 ± 0.723
6.584 ± 1.036
6.584 ± 0.641
1.739 ± 0.006
3.727 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here