Enterobacter phage phiEap-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Cornellvirus; unclassified Cornellvirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2FHN5|A0A0K2FHN5_9CAUD Uncharacterized protein OS=Enterobacter phage phiEap-2 OX=1701257 GN=ABY59_0200025 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.36LDD4 pKa = 3.63VGKK7 pKa = 10.65LIYY10 pKa = 10.73AFIYY14 pKa = 10.73NGFSEE19 pKa = 4.67KK20 pKa = 10.7DD21 pKa = 3.21AFNAAFDD28 pKa = 3.86YY29 pKa = 10.75RR30 pKa = 11.84NSVEE34 pKa = 4.82IFDD37 pKa = 5.02IEE39 pKa = 4.14NQIDD43 pKa = 4.29LLMIANFSQDD53 pKa = 3.21MYY55 pKa = 11.28NEE57 pKa = 4.98AIAEE61 pKa = 4.2EE62 pKa = 4.52LEE64 pKa = 3.75HH65 pKa = 6.93WYY67 pKa = 10.59

Molecular weight: 7.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2FIS5|A0A0K2FIS5_9CAUD Uncharacterized protein OS=Enterobacter phage phiEap-2 OX=1701257 GN=ABY59_0200054 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84LIPKK6 pKa = 9.07QKK8 pKa = 9.91KK9 pKa = 9.1RR10 pKa = 11.84CIKK13 pKa = 10.17CDD15 pKa = 3.23EE16 pKa = 4.28VKK18 pKa = 10.45PLSEE22 pKa = 4.08FHH24 pKa = 6.68RR25 pKa = 11.84SSVASKK31 pKa = 10.17DD32 pKa = 3.36GHH34 pKa = 6.05RR35 pKa = 11.84NEE37 pKa = 5.35CIKK40 pKa = 10.53CRR42 pKa = 11.84RR43 pKa = 11.84KK44 pKa = 9.95IEE46 pKa = 3.79AARR49 pKa = 11.84IRR51 pKa = 11.84EE52 pKa = 3.99KK53 pKa = 10.79RR54 pKa = 11.84GAKK57 pKa = 9.45

Molecular weight: 6.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12154

44

838

196.0

21.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.425 ± 0.577

1.094 ± 0.128

6.36 ± 0.262

6.212 ± 0.371

3.489 ± 0.163

7.726 ± 0.357

1.761 ± 0.232

5.488 ± 0.258

5.998 ± 0.439

7.775 ± 0.344

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.46 ± 0.146

4.863 ± 0.324

3.982 ± 0.272

4.089 ± 0.378

5.077 ± 0.281

5.858 ± 0.351

6.204 ± 0.367

6.599 ± 0.36

1.514 ± 0.155

3.028 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski