Amino acid dipepetide frequency for Enterobacter phage phiEap-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.836AlaAla: 12.836 ± 1.626
0.905AlaCys: 0.905 ± 0.246
6.994AlaAsp: 6.994 ± 0.704
7.076AlaGlu: 7.076 ± 0.768
4.032AlaPhe: 4.032 ± 0.66
9.051AlaGly: 9.051 ± 0.829
1.893AlaHis: 1.893 ± 0.359
6.665AlaIle: 6.665 ± 0.775
6.5AlaLys: 6.5 ± 1.004
8.475AlaLeu: 8.475 ± 0.876
2.057AlaMet: 2.057 ± 0.459
4.279AlaAsn: 4.279 ± 0.535
2.798AlaPro: 2.798 ± 0.557
3.456AlaGln: 3.456 ± 0.739
4.443AlaArg: 4.443 ± 0.547
5.842AlaSer: 5.842 ± 0.735
5.76AlaThr: 5.76 ± 0.719
6.665AlaVal: 6.665 ± 0.667
1.399AlaTrp: 1.399 ± 0.303
3.127AlaTyr: 3.127 ± 0.442
0.0AlaXaa: 0.0 ± 0.0
Cys
1.07CysAla: 1.07 ± 0.351
0.082CysCys: 0.082 ± 0.072
0.987CysAsp: 0.987 ± 0.276
1.07CysGlu: 1.07 ± 0.383
0.165CysPhe: 0.165 ± 0.112
0.987CysGly: 0.987 ± 0.262
0.165CysHis: 0.165 ± 0.085
0.494CysIle: 0.494 ± 0.228
0.658CysLys: 0.658 ± 0.188
0.741CysLeu: 0.741 ± 0.231
0.165CysMet: 0.165 ± 0.106
0.823CysAsn: 0.823 ± 0.253
0.494CysPro: 0.494 ± 0.169
0.658CysGln: 0.658 ± 0.264
0.741CysArg: 0.741 ± 0.274
0.165CysSer: 0.165 ± 0.111
0.247CysThr: 0.247 ± 0.134
0.576CysVal: 0.576 ± 0.248
0.165CysTrp: 0.165 ± 0.098
0.494CysTyr: 0.494 ± 0.184
0.0CysXaa: 0.0 ± 0.0
Asp
7.159AspAla: 7.159 ± 0.563
0.247AspCys: 0.247 ± 0.15
4.69AspAsp: 4.69 ± 0.802
5.102AspGlu: 5.102 ± 0.759
2.139AspPhe: 2.139 ± 0.379
5.924AspGly: 5.924 ± 0.86
1.152AspHis: 1.152 ± 0.324
4.032AspIle: 4.032 ± 0.486
3.867AspLys: 3.867 ± 0.638
5.924AspLeu: 5.924 ± 0.74
1.646AspMet: 1.646 ± 0.353
2.222AspAsn: 2.222 ± 0.387
1.975AspPro: 1.975 ± 0.487
1.728AspGln: 1.728 ± 0.501
2.386AspArg: 2.386 ± 0.41
4.361AspSer: 4.361 ± 0.568
3.456AspThr: 3.456 ± 0.484
3.291AspVal: 3.291 ± 0.605
0.987AspTrp: 0.987 ± 0.238
1.317AspTyr: 1.317 ± 0.26
0.0AspXaa: 0.0 ± 0.0
Glu
6.089GluAla: 6.089 ± 0.958
0.576GluCys: 0.576 ± 0.259
4.196GluAsp: 4.196 ± 0.602
4.196GluGlu: 4.196 ± 0.718
2.798GluPhe: 2.798 ± 0.453
3.867GluGly: 3.867 ± 0.499
1.07GluHis: 1.07 ± 0.212
3.127GluIle: 3.127 ± 0.471
4.279GluLys: 4.279 ± 0.639
6.171GluLeu: 6.171 ± 0.927
2.304GluMet: 2.304 ± 0.49
2.304GluAsn: 2.304 ± 0.33
2.469GluPro: 2.469 ± 0.508
2.469GluGln: 2.469 ± 0.494
3.621GluArg: 3.621 ± 0.517
3.127GluSer: 3.127 ± 0.492
2.715GluThr: 2.715 ± 0.556
3.374GluVal: 3.374 ± 0.519
0.987GluTrp: 0.987 ± 0.243
2.386GluTyr: 2.386 ± 0.504
0.0GluXaa: 0.0 ± 0.0
Phe
2.304PheAla: 2.304 ± 0.404
0.411PheCys: 0.411 ± 0.192
2.715PheAsp: 2.715 ± 0.487
2.386PheGlu: 2.386 ± 0.413
1.152PhePhe: 1.152 ± 0.344
3.209PheGly: 3.209 ± 0.502
0.823PheHis: 0.823 ± 0.288
2.386PheIle: 2.386 ± 0.399
1.152PheLys: 1.152 ± 0.264
2.222PheLeu: 2.222 ± 0.4
0.658PheMet: 0.658 ± 0.222
1.975PheAsn: 1.975 ± 0.406
0.987PhePro: 0.987 ± 0.267
0.905PheGln: 0.905 ± 0.216
1.728PheArg: 1.728 ± 0.31
2.551PheSer: 2.551 ± 0.429
2.715PheThr: 2.715 ± 0.519
2.715PheVal: 2.715 ± 0.437
0.741PheTrp: 0.741 ± 0.249
1.152PheTyr: 1.152 ± 0.24
0.0PheXaa: 0.0 ± 0.0
Gly
7.076GlyAla: 7.076 ± 1.105
1.234GlyCys: 1.234 ± 0.449
4.361GlyAsp: 4.361 ± 0.561
3.785GlyGlu: 3.785 ± 0.518
2.88GlyPhe: 2.88 ± 0.468
5.678GlyGly: 5.678 ± 0.647
1.234GlyHis: 1.234 ± 0.275
3.95GlyIle: 3.95 ± 0.7
5.102GlyLys: 5.102 ± 0.87
4.937GlyLeu: 4.937 ± 0.747
1.728GlyMet: 1.728 ± 0.381
4.279GlyAsn: 4.279 ± 0.665
1.893GlyPro: 1.893 ± 0.452
3.867GlyGln: 3.867 ± 0.551
4.443GlyArg: 4.443 ± 0.646
5.842GlySer: 5.842 ± 0.708
5.019GlyThr: 5.019 ± 0.634
6.089GlyVal: 6.089 ± 0.677
1.317GlyTrp: 1.317 ± 0.345
2.551GlyTyr: 2.551 ± 0.348
0.0GlyXaa: 0.0 ± 0.0
His
2.222HisAla: 2.222 ± 0.403
0.329HisCys: 0.329 ± 0.174
1.234HisAsp: 1.234 ± 0.302
1.07HisGlu: 1.07 ± 0.338
0.411HisPhe: 0.411 ± 0.182
1.317HisGly: 1.317 ± 0.301
0.411HisHis: 0.411 ± 0.163
2.057HisIle: 2.057 ± 0.477
1.234HisLys: 1.234 ± 0.331
0.823HisLeu: 0.823 ± 0.196
0.247HisMet: 0.247 ± 0.147
0.741HisAsn: 0.741 ± 0.242
1.317HisPro: 1.317 ± 0.374
1.07HisGln: 1.07 ± 0.241
0.987HisArg: 0.987 ± 0.262
0.741HisSer: 0.741 ± 0.223
0.329HisThr: 0.329 ± 0.162
0.411HisVal: 0.411 ± 0.208
0.329HisTrp: 0.329 ± 0.155
0.329HisTyr: 0.329 ± 0.164
0.0HisXaa: 0.0 ± 0.0
Ile
5.348IleAla: 5.348 ± 0.721
0.658IleCys: 0.658 ± 0.214
3.95IleAsp: 3.95 ± 0.52
3.209IleGlu: 3.209 ± 0.537
1.481IlePhe: 1.481 ± 0.291
4.443IleGly: 4.443 ± 0.567
0.494IleHis: 0.494 ± 0.188
2.88IleIle: 2.88 ± 0.408
3.867IleLys: 3.867 ± 0.611
3.045IleLeu: 3.045 ± 0.493
1.481IleMet: 1.481 ± 0.401
3.785IleAsn: 3.785 ± 0.651
2.798IlePro: 2.798 ± 0.52
1.893IleGln: 1.893 ± 0.324
2.551IleArg: 2.551 ± 0.34
3.045IleSer: 3.045 ± 0.596
4.196IleThr: 4.196 ± 0.538
3.703IleVal: 3.703 ± 0.435
0.823IleTrp: 0.823 ± 0.226
1.234IleTyr: 1.234 ± 0.314
0.0IleXaa: 0.0 ± 0.0
Lys
5.842LysAla: 5.842 ± 0.877
0.823LysCys: 0.823 ± 0.287
2.633LysAsp: 2.633 ± 0.458
3.703LysGlu: 3.703 ± 0.532
1.81LysPhe: 1.81 ± 0.377
4.114LysGly: 4.114 ± 0.632
1.317LysHis: 1.317 ± 0.276
1.81LysIle: 1.81 ± 0.331
3.374LysLys: 3.374 ± 0.498
5.431LysLeu: 5.431 ± 0.699
2.798LysMet: 2.798 ± 0.525
2.551LysAsn: 2.551 ± 0.525
3.785LysPro: 3.785 ± 0.576
3.209LysGln: 3.209 ± 0.614
3.374LysArg: 3.374 ± 0.529
3.209LysSer: 3.209 ± 0.66
3.374LysThr: 3.374 ± 0.46
3.127LysVal: 3.127 ± 0.495
0.905LysTrp: 0.905 ± 0.221
2.798LysTyr: 2.798 ± 0.516
0.0LysXaa: 0.0 ± 0.0
Leu
7.735LeuAla: 7.735 ± 0.767
1.317LeuCys: 1.317 ± 0.324
5.184LeuAsp: 5.184 ± 0.663
5.102LeuGlu: 5.102 ± 0.677
2.304LeuPhe: 2.304 ± 0.445
4.114LeuGly: 4.114 ± 0.438
1.234LeuHis: 1.234 ± 0.349
4.443LeuIle: 4.443 ± 0.624
5.76LeuLys: 5.76 ± 0.621
6.089LeuLeu: 6.089 ± 0.676
1.893LeuMet: 1.893 ± 0.314
4.772LeuAsn: 4.772 ± 0.595
3.291LeuPro: 3.291 ± 0.514
3.374LeuGln: 3.374 ± 0.54
3.867LeuArg: 3.867 ± 0.518
4.772LeuSer: 4.772 ± 0.748
5.595LeuThr: 5.595 ± 0.729
4.69LeuVal: 4.69 ± 0.708
0.741LeuTrp: 0.741 ± 0.242
1.481LeuTyr: 1.481 ± 0.323
0.0LeuXaa: 0.0 ± 0.0
Met
3.374MetAla: 3.374 ± 0.528
0.411MetCys: 0.411 ± 0.161
0.494MetAsp: 0.494 ± 0.256
2.057MetGlu: 2.057 ± 0.372
0.658MetPhe: 0.658 ± 0.216
1.481MetGly: 1.481 ± 0.26
0.247MetHis: 0.247 ± 0.108
0.905MetIle: 0.905 ± 0.303
1.152MetLys: 1.152 ± 0.286
1.975MetLeu: 1.975 ± 0.349
0.905MetMet: 0.905 ± 0.24
0.741MetAsn: 0.741 ± 0.198
1.563MetPro: 1.563 ± 0.332
0.987MetGln: 0.987 ± 0.241
1.563MetArg: 1.563 ± 0.308
1.234MetSer: 1.234 ± 0.308
2.222MetThr: 2.222 ± 0.436
1.893MetVal: 1.893 ± 0.41
0.247MetTrp: 0.247 ± 0.139
0.494MetTyr: 0.494 ± 0.18
0.0MetXaa: 0.0 ± 0.0
Asn
4.772AsnAla: 4.772 ± 0.8
0.576AsnCys: 0.576 ± 0.213
2.715AsnAsp: 2.715 ± 0.361
2.304AsnGlu: 2.304 ± 0.372
1.317AsnPhe: 1.317 ± 0.448
4.443AsnGly: 4.443 ± 0.622
1.317AsnHis: 1.317 ± 0.339
2.304AsnIle: 2.304 ± 0.47
3.127AsnLys: 3.127 ± 0.507
3.045AsnLeu: 3.045 ± 0.448
1.152AsnMet: 1.152 ± 0.258
3.127AsnAsn: 3.127 ± 0.746
1.893AsnPro: 1.893 ± 0.355
2.057AsnGln: 2.057 ± 0.358
2.633AsnArg: 2.633 ± 0.523
2.88AsnSer: 2.88 ± 0.497
2.798AsnThr: 2.798 ± 0.614
3.291AsnVal: 3.291 ± 0.533
1.234AsnTrp: 1.234 ± 0.256
1.646AsnTyr: 1.646 ± 0.285
0.0AsnXaa: 0.0 ± 0.0
Pro
4.443ProAla: 4.443 ± 0.589
0.329ProCys: 0.329 ± 0.151
3.209ProAsp: 3.209 ± 0.55
3.291ProGlu: 3.291 ± 0.527
1.646ProPhe: 1.646 ± 0.385
3.703ProGly: 3.703 ± 0.549
0.247ProHis: 0.247 ± 0.133
1.563ProIle: 1.563 ± 0.303
1.399ProLys: 1.399 ± 0.449
3.703ProLeu: 3.703 ± 0.533
0.905ProMet: 0.905 ± 0.245
1.893ProAsn: 1.893 ± 0.438
0.576ProPro: 0.576 ± 0.198
1.234ProGln: 1.234 ± 0.358
1.81ProArg: 1.81 ± 0.333
1.975ProSer: 1.975 ± 0.439
2.715ProThr: 2.715 ± 0.354
3.621ProVal: 3.621 ± 0.641
0.329ProTrp: 0.329 ± 0.262
1.234ProTyr: 1.234 ± 0.353
0.0ProXaa: 0.0 ± 0.0
Gln
5.431GlnAla: 5.431 ± 0.656
0.741GlnCys: 0.741 ± 0.245
1.893GlnAsp: 1.893 ± 0.378
1.893GlnGlu: 1.893 ± 0.317
1.399GlnPhe: 1.399 ± 0.289
2.139GlnGly: 2.139 ± 0.599
0.741GlnHis: 0.741 ± 0.303
2.88GlnIle: 2.88 ± 0.593
2.551GlnLys: 2.551 ± 0.643
2.88GlnLeu: 2.88 ± 0.394
1.399GlnMet: 1.399 ± 0.286
1.234GlnAsn: 1.234 ± 0.422
1.975GlnPro: 1.975 ± 0.4
2.304GlnGln: 2.304 ± 0.625
2.386GlnArg: 2.386 ± 0.411
1.728GlnSer: 1.728 ± 0.466
2.551GlnThr: 2.551 ± 0.478
2.798GlnVal: 2.798 ± 0.471
0.411GlnTrp: 0.411 ± 0.16
1.563GlnTyr: 1.563 ± 0.319
0.0GlnXaa: 0.0 ± 0.0
Arg
4.608ArgAla: 4.608 ± 0.555
0.411ArgCys: 0.411 ± 0.167
2.88ArgAsp: 2.88 ± 0.582
3.127ArgGlu: 3.127 ± 0.644
1.81ArgPhe: 1.81 ± 0.35
3.621ArgGly: 3.621 ± 0.524
1.07ArgHis: 1.07 ± 0.299
2.88ArgIle: 2.88 ± 0.371
3.456ArgLys: 3.456 ± 0.48
4.114ArgLeu: 4.114 ± 0.452
1.152ArgMet: 1.152 ± 0.294
2.798ArgAsn: 2.798 ± 0.564
2.139ArgPro: 2.139 ± 0.44
2.469ArgGln: 2.469 ± 0.471
3.045ArgArg: 3.045 ± 0.463
2.469ArgSer: 2.469 ± 0.363
2.304ArgThr: 2.304 ± 0.429
4.032ArgVal: 4.032 ± 0.548
0.987ArgTrp: 0.987 ± 0.3
1.399ArgTyr: 1.399 ± 0.348
0.0ArgXaa: 0.0 ± 0.0
Ser
6.089SerAla: 6.089 ± 1.006
0.247SerCys: 0.247 ± 0.116
3.374SerAsp: 3.374 ± 0.492
2.386SerGlu: 2.386 ± 0.443
2.551SerPhe: 2.551 ± 0.337
6.089SerGly: 6.089 ± 0.913
1.399SerHis: 1.399 ± 0.407
2.962SerIle: 2.962 ± 0.424
3.045SerLys: 3.045 ± 0.569
4.196SerLeu: 4.196 ± 0.59
0.987SerMet: 0.987 ± 0.368
2.88SerAsn: 2.88 ± 0.447
2.469SerPro: 2.469 ± 0.454
2.139SerGln: 2.139 ± 0.409
3.045SerArg: 3.045 ± 0.413
3.456SerSer: 3.456 ± 0.511
4.361SerThr: 4.361 ± 0.68
4.526SerVal: 4.526 ± 0.727
0.658SerTrp: 0.658 ± 0.195
1.646SerTyr: 1.646 ± 0.422
0.0SerXaa: 0.0 ± 0.0
Thr
6.912ThrAla: 6.912 ± 0.752
0.494ThrCys: 0.494 ± 0.194
3.703ThrAsp: 3.703 ± 0.578
3.291ThrGlu: 3.291 ± 0.562
2.715ThrPhe: 2.715 ± 0.535
6.336ThrGly: 6.336 ± 0.836
0.494ThrHis: 0.494 ± 0.163
3.538ThrIle: 3.538 ± 0.491
2.798ThrLys: 2.798 ± 0.402
4.69ThrLeu: 4.69 ± 0.517
0.905ThrMet: 0.905 ± 0.252
2.715ThrAsn: 2.715 ± 0.509
3.456ThrPro: 3.456 ± 0.512
2.222ThrGln: 2.222 ± 0.397
2.715ThrArg: 2.715 ± 0.454
3.785ThrSer: 3.785 ± 0.559
4.279ThrThr: 4.279 ± 0.658
4.114ThrVal: 4.114 ± 0.724
0.905ThrTrp: 0.905 ± 0.281
1.975ThrTyr: 1.975 ± 0.459
0.0ThrXaa: 0.0 ± 0.0
Val
6.912ValAla: 6.912 ± 0.709
0.494ValCys: 0.494 ± 0.257
4.443ValAsp: 4.443 ± 0.568
4.279ValGlu: 4.279 ± 0.606
1.563ValPhe: 1.563 ± 0.416
3.785ValGly: 3.785 ± 0.602
1.481ValHis: 1.481 ± 0.391
4.032ValIle: 4.032 ± 0.489
4.772ValLys: 4.772 ± 0.665
4.69ValLeu: 4.69 ± 0.638
1.563ValMet: 1.563 ± 0.413
3.538ValAsn: 3.538 ± 0.514
2.139ValPro: 2.139 ± 0.303
2.386ValGln: 2.386 ± 0.523
2.88ValArg: 2.88 ± 0.46
4.361ValSer: 4.361 ± 0.642
5.102ValThr: 5.102 ± 0.659
4.443ValVal: 4.443 ± 0.711
1.07ValTrp: 1.07 ± 0.308
2.057ValTyr: 2.057 ± 0.436
0.0ValXaa: 0.0 ± 0.0
Trp
1.234TrpAla: 1.234 ± 0.421
0.165TrpCys: 0.165 ± 0.093
1.234TrpAsp: 1.234 ± 0.324
0.576TrpGlu: 0.576 ± 0.233
0.741TrpPhe: 0.741 ± 0.236
0.905TrpGly: 0.905 ± 0.303
0.658TrpHis: 0.658 ± 0.182
0.411TrpIle: 0.411 ± 0.197
0.658TrpLys: 0.658 ± 0.285
1.563TrpLeu: 1.563 ± 0.38
0.165TrpMet: 0.165 ± 0.105
0.576TrpAsn: 0.576 ± 0.222
0.658TrpPro: 0.658 ± 0.24
0.987TrpGln: 0.987 ± 0.248
0.987TrpArg: 0.987 ± 0.234
0.905TrpSer: 0.905 ± 0.259
0.741TrpThr: 0.741 ± 0.196
0.905TrpVal: 0.905 ± 0.261
0.411TrpTrp: 0.411 ± 0.225
0.658TrpTyr: 0.658 ± 0.247
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.798TyrAla: 2.798 ± 0.489
0.494TyrCys: 0.494 ± 0.205
2.715TyrAsp: 2.715 ± 0.425
2.222TyrGlu: 2.222 ± 0.456
1.317TyrPhe: 1.317 ± 0.329
2.057TyrGly: 2.057 ± 0.443
0.165TyrHis: 0.165 ± 0.097
1.563TyrIle: 1.563 ± 0.314
1.234TyrLys: 1.234 ± 0.205
3.045TyrLeu: 3.045 ± 0.483
0.411TyrMet: 0.411 ± 0.169
1.399TyrAsn: 1.399 ± 0.355
1.152TyrPro: 1.152 ± 0.28
1.481TyrGln: 1.481 ± 0.417
1.563TyrArg: 1.563 ± 0.289
2.139TyrSer: 2.139 ± 0.452
1.563TyrThr: 1.563 ± 0.385
1.728TyrVal: 1.728 ± 0.375
0.494TyrTrp: 0.494 ± 0.164
0.741TyrTyr: 0.741 ± 0.228
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (12154 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski