Planococcus halotolerans
Average proteome isoelectric point is 5.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3547 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A365KRL2|A0A365KRL2_9BACL Transcriptional regulator OS=Planococcus halotolerans OX=2233542 GN=DP120_13310 PE=4 SV=1
MM1 pKa = 7.42 KK2 pKa = 10.13 KK3 pKa = 10.15 KK4 pKa = 10.68 AIGVLATSALILAGCGSTGGEE25 pKa = 3.79 EE26 pKa = 4.15 SEE28 pKa = 4.9 PIVIGGKK35 pKa = 8.9 PWTEE39 pKa = 4.0 QYY41 pKa = 10.81 ILPHH45 pKa = 7.07 ILGQYY50 pKa = 9.7 IEE52 pKa = 5.03 ANSDD56 pKa = 3.49 YY57 pKa = 10.43 TVEE60 pKa = 4.22 YY61 pKa = 10.34 EE62 pKa = 4.31 DD63 pKa = 4.27 GLGEE67 pKa = 4.39 VSILTPALEE76 pKa = 4.14 QGDD79 pKa = 3.22 IDD81 pKa = 6.02 LYY83 pKa = 11.36 VEE85 pKa = 4.11 YY86 pKa = 9.92 TGTGLKK92 pKa = 10.37 DD93 pKa = 3.33 VLKK96 pKa = 10.72 QEE98 pKa = 4.44 SEE100 pKa = 4.16 PGQSSEE106 pKa = 4.38 EE107 pKa = 3.72 VLQRR111 pKa = 11.84 VRR113 pKa = 11.84 EE114 pKa = 4.56 GYY116 pKa = 10.24 EE117 pKa = 3.78 EE118 pKa = 4.14 EE119 pKa = 5.86 LNATWLEE126 pKa = 3.82 PLGFEE131 pKa = 4.79 NGYY134 pKa = 7.77 TLAYY138 pKa = 10.14 SKK140 pKa = 11.15 DD141 pKa = 3.36 SGYY144 pKa = 10.96 DD145 pKa = 3.08 AEE147 pKa = 4.91 TYY149 pKa = 11.0 SDD151 pKa = 3.91 LAEE154 pKa = 4.29 ISQTEE159 pKa = 4.27 DD160 pKa = 2.94 MSFGAPHH167 pKa = 7.25 PFYY170 pKa = 10.73 EE171 pKa = 4.37 RR172 pKa = 11.84 QGDD175 pKa = 4.35 GYY177 pKa = 11.06 DD178 pKa = 4.39 DD179 pKa = 6.31 LIATYY184 pKa = 9.54 PFEE187 pKa = 5.16 FSATDD192 pKa = 3.43 SFDD195 pKa = 3.54 PAIMYY200 pKa = 7.64 EE201 pKa = 4.0 AVNNGEE207 pKa = 4.01 VDD209 pKa = 4.12 VIPAFTTDD217 pKa = 2.92 SRR219 pKa = 11.84 IGLFDD224 pKa = 5.22 LEE226 pKa = 4.5 TTEE229 pKa = 5.62 DD230 pKa = 3.96 DD231 pKa = 4.31 LSFFPKK237 pKa = 9.98 YY238 pKa = 10.1 DD239 pKa = 3.42 AAPVVRR245 pKa = 11.84 LEE247 pKa = 4.41 ALEE250 pKa = 4.09 QYY252 pKa = 10.55 PEE254 pKa = 4.37 LEE256 pKa = 4.11 SLLNEE261 pKa = 4.02 LAGQISEE268 pKa = 4.42 EE269 pKa = 4.1 EE270 pKa = 4.08 MLAMNARR277 pKa = 11.84 VDD279 pKa = 3.41 IDD281 pKa = 3.25 QDD283 pKa = 3.68 QPQDD287 pKa = 3.03 IARR290 pKa = 11.84 DD291 pKa = 3.63 FLIEE295 pKa = 4.06 KK296 pKa = 10.46 GLIEE300 pKa = 4.14 EE301 pKa = 4.41
Molecular weight: 33.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.77
IPC_protein 3.757
Toseland 3.567
ProMoST 3.872
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.605
Rodwell 3.579
Grimsley 3.478
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 3.986
Thurlkill 3.592
EMBOSS 3.617
Sillero 3.872
Patrickios 1.252
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.766
Protein with the highest isoelectric point:
>tr|A0A365L8K6|A0A365L8K6_9BACL DNA mismatch repair protein MutT OS=Planococcus halotolerans OX=2233542 GN=DP120_03900 PE=4 SV=1
MM1 pKa = 7.61 TLRR4 pKa = 11.84 TYY6 pKa = 10.57 QPNKK10 pKa = 8.74 RR11 pKa = 11.84 KK12 pKa = 9.54 HH13 pKa = 5.99 SKK15 pKa = 8.91 VHH17 pKa = 5.68 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MSTKK26 pKa = 9.71 NGRR29 pKa = 11.84 RR30 pKa = 11.84 VIAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 8.8 GRR40 pKa = 11.84 KK41 pKa = 8.75 VLSAA45 pKa = 4.05
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3547
0
3547
1025973
34
2657
289.3
32.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.008 ± 0.043
0.56 ± 0.011
5.395 ± 0.034
8.087 ± 0.055
4.602 ± 0.035
7.139 ± 0.039
1.971 ± 0.02
7.305 ± 0.038
6.151 ± 0.034
9.679 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.936 ± 0.021
4.008 ± 0.029
3.685 ± 0.023
3.55 ± 0.025
4.204 ± 0.033
5.986 ± 0.029
5.386 ± 0.036
6.922 ± 0.03
1.05 ± 0.014
3.374 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here