Listonella phage phiHSIC

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5G7N8|Q5G7N8_9CAUD Putative tail tape measure protein OS=Listonella phage phiHSIC OX=310539 GN=ORF31 PE=4 SV=1
MM1 pKa = 7.19SLKK4 pKa = 10.47VVDD7 pKa = 5.05PIEE10 pKa = 3.98VTEE13 pKa = 4.26AVLTASGIPEE23 pKa = 4.31PDD25 pKa = 3.08VSQGEE30 pKa = 4.42VEE32 pKa = 4.35YY33 pKa = 11.22NPNKK37 pKa = 9.8TEE39 pKa = 4.56GIKK42 pKa = 9.25FTNVTWDD49 pKa = 2.95ITDD52 pKa = 3.57IARR55 pKa = 11.84DD56 pKa = 3.35GGTIYY61 pKa = 11.18ALDD64 pKa = 4.64EE65 pKa = 4.62YY66 pKa = 10.31PFSPSGASLVRR77 pKa = 11.84TYY79 pKa = 11.51DD80 pKa = 3.21VFGNEE85 pKa = 3.53TAIAFSTQNDD95 pKa = 3.77AKK97 pKa = 11.02CIDD100 pKa = 3.88VYY102 pKa = 11.06LGSIYY107 pKa = 11.06VGDD110 pKa = 3.73SNGFVNKK117 pKa = 10.16YY118 pKa = 6.62NTSGTLLEE126 pKa = 4.64TFDD129 pKa = 3.49LTANVSRR136 pKa = 11.84VDD138 pKa = 4.74AICGSYY144 pKa = 10.85GPFYY148 pKa = 10.66ILDD151 pKa = 3.55QTTGKK156 pKa = 9.95VFEE159 pKa = 4.91FGTLNDD165 pKa = 3.65LTPFEE170 pKa = 4.41VRR172 pKa = 11.84DD173 pKa = 4.0FGTFGSFIFGFTHH186 pKa = 6.76RR187 pKa = 11.84NGIIYY192 pKa = 7.42YY193 pKa = 8.73TNQNNGKK200 pKa = 8.63IEE202 pKa = 4.23SMSAGTGEE210 pKa = 4.32ARR212 pKa = 11.84DD213 pKa = 4.01SFALTNDD220 pKa = 3.31RR221 pKa = 11.84DD222 pKa = 3.66GVYY225 pKa = 10.77YY226 pKa = 10.91LGGLAFLADD235 pKa = 3.96GSLLSVNTTGSEE247 pKa = 3.7AFKK250 pKa = 10.86YY251 pKa = 10.89VNGSLKK257 pKa = 10.29QPYY260 pKa = 9.7NPGDD264 pKa = 3.58QVVVLSNHH272 pKa = 5.65TKK274 pKa = 9.04YY275 pKa = 10.29QCLVATSQSPIDD287 pKa = 4.28GATEE291 pKa = 3.92DD292 pKa = 5.01ATATWIKK299 pKa = 10.63VGPTNKK305 pKa = 9.01WAMFDD310 pKa = 3.47NLQNTKK316 pKa = 8.39TLNDD320 pKa = 3.3TDD322 pKa = 4.05FTITLNPTTYY332 pKa = 10.85VNTLAALGFSGVTSIRR348 pKa = 11.84VEE350 pKa = 3.77VDD352 pKa = 2.88NVGGTTIYY360 pKa = 10.94DD361 pKa = 3.64KK362 pKa = 11.36TFGTSDD368 pKa = 3.42FSAIYY373 pKa = 10.4DD374 pKa = 3.39HH375 pKa = 6.09YY376 pKa = 10.06TYY378 pKa = 11.36VFYY381 pKa = 10.9QIVALQKK388 pKa = 10.88LIVDD392 pKa = 5.03DD393 pKa = 5.52LPPLPDD399 pKa = 3.21TEE401 pKa = 4.08IRR403 pKa = 11.84VTFSGSDD410 pKa = 3.22IQIGEE415 pKa = 4.37LAHH418 pKa = 6.68GFAINVGTLVAEE430 pKa = 4.39STKK433 pKa = 10.51SDD435 pKa = 3.04RR436 pKa = 11.84FRR438 pKa = 11.84YY439 pKa = 9.69RR440 pKa = 11.84EE441 pKa = 3.59QQYY444 pKa = 10.89NEE446 pKa = 3.55FGYY449 pKa = 9.9PVGAEE454 pKa = 4.15PIVVEE459 pKa = 4.23LNTYY463 pKa = 10.43DD464 pKa = 3.81VLVPKK469 pKa = 10.43LNNPAIQKK477 pKa = 10.41LLDD480 pKa = 3.66RR481 pKa = 11.84LTGKK485 pKa = 8.23NTLWVGDD492 pKa = 3.53IGGGQGLITYY502 pKa = 8.02GFFEE506 pKa = 4.79RR507 pKa = 11.84SPIPFSMPYY516 pKa = 10.0DD517 pKa = 3.28INYY520 pKa = 9.7QITVRR525 pKa = 11.84ASVV528 pKa = 2.9

Molecular weight: 57.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5G7P5|Q5G7P5_9CAUD Hypothetical phage protein OS=Listonella phage phiHSIC OX=310539 GN=ORF24 PE=4 SV=1
MM1 pKa = 7.63LSCICGGNPMSWSKK15 pKa = 10.79SLKK18 pKa = 10.42NIIVKK23 pKa = 10.4NEE25 pKa = 3.65NLTEE29 pKa = 4.04KK30 pKa = 10.41QLRR33 pKa = 11.84AGLFDD38 pKa = 4.03AANTVILGSPVGAPEE53 pKa = 5.09LWQQPAPNYY62 pKa = 9.72YY63 pKa = 9.66RR64 pKa = 11.84AGSYY68 pKa = 10.16RR69 pKa = 11.84SNHH72 pKa = 5.7RR73 pKa = 11.84VSISKK78 pKa = 8.29ITSFEE83 pKa = 4.06KK84 pKa = 10.87GISSQSSIMMDD95 pKa = 4.02LQSDD99 pKa = 3.55IAKK102 pKa = 10.16FKK104 pKa = 10.6IGEE107 pKa = 4.39TLFMTNPLPYY117 pKa = 9.03ATSIEE122 pKa = 4.36YY123 pKa = 10.21GHH125 pKa = 6.67SSQAPNGVYY134 pKa = 9.86RR135 pKa = 11.84PAVRR139 pKa = 11.84RR140 pKa = 11.84LVKK143 pKa = 10.39FLNTEE148 pKa = 4.55LKK150 pKa = 10.81AKK152 pKa = 10.3

Molecular weight: 16.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

11099

49

1069

236.1

26.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.226 ± 0.608

1.505 ± 0.269

6.118 ± 0.236

6.92 ± 0.372

4.036 ± 0.181

7.028 ± 0.349

1.739 ± 0.223

5.928 ± 0.239

6.902 ± 0.435

7.947 ± 0.354

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.874 ± 0.209

5.28 ± 0.257

3.559 ± 0.256

3.721 ± 0.284

3.982 ± 0.25

6.965 ± 0.388

5.739 ± 0.364

6.631 ± 0.257

1.189 ± 0.159

3.694 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski