Amino acid dipepetide frequency for Listonella phage phiHSIC

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.29AlaAla: 8.29 ± 1.399
0.541AlaCys: 0.541 ± 0.186
3.965AlaAsp: 3.965 ± 0.683
6.307AlaGlu: 6.307 ± 0.729
2.974AlaPhe: 2.974 ± 0.65
4.686AlaGly: 4.686 ± 0.779
1.712AlaHis: 1.712 ± 0.477
5.587AlaIle: 5.587 ± 0.858
6.578AlaLys: 6.578 ± 0.792
7.749AlaLeu: 7.749 ± 1.191
3.064AlaMet: 3.064 ± 0.818
4.145AlaAsn: 4.145 ± 0.815
2.974AlaPro: 2.974 ± 0.642
3.244AlaGln: 3.244 ± 0.541
3.064AlaArg: 3.064 ± 0.68
4.235AlaSer: 4.235 ± 0.656
5.046AlaThr: 5.046 ± 0.975
4.776AlaVal: 4.776 ± 0.507
0.451AlaTrp: 0.451 ± 0.199
2.793AlaTyr: 2.793 ± 0.528
0.09AlaXaa: 0.09 ± 0.096
Cys
0.36CysAla: 0.36 ± 0.177
0.541CysCys: 0.541 ± 0.242
0.811CysAsp: 0.811 ± 0.267
0.991CysGlu: 0.991 ± 0.278
0.631CysPhe: 0.631 ± 0.249
1.532CysGly: 1.532 ± 0.315
0.36CysHis: 0.36 ± 0.159
1.171CysIle: 1.171 ± 0.307
1.081CysLys: 1.081 ± 0.329
0.991CysLeu: 0.991 ± 0.332
0.18CysMet: 0.18 ± 0.116
0.18CysAsn: 0.18 ± 0.125
0.541CysPro: 0.541 ± 0.217
0.721CysGln: 0.721 ± 0.298
0.811CysArg: 0.811 ± 0.327
0.991CysSer: 0.991 ± 0.293
0.811CysThr: 0.811 ± 0.287
1.171CysVal: 1.171 ± 0.389
0.36CysTrp: 0.36 ± 0.219
0.811CysTyr: 0.811 ± 0.324
0.0CysXaa: 0.0 ± 0.0
Asp
5.767AspAla: 5.767 ± 0.852
0.811AspCys: 0.811 ± 0.258
3.514AspAsp: 3.514 ± 0.555
4.776AspGlu: 4.776 ± 0.768
2.523AspPhe: 2.523 ± 0.463
5.316AspGly: 5.316 ± 0.644
1.261AspHis: 1.261 ± 0.428
3.965AspIle: 3.965 ± 0.586
4.235AspLys: 4.235 ± 0.662
4.776AspLeu: 4.776 ± 0.572
1.442AspMet: 1.442 ± 0.422
3.064AspAsn: 3.064 ± 0.53
1.892AspPro: 1.892 ± 0.34
1.622AspGln: 1.622 ± 0.346
2.163AspArg: 2.163 ± 0.445
3.244AspSer: 3.244 ± 0.593
3.064AspThr: 3.064 ± 0.413
4.145AspVal: 4.145 ± 0.617
0.991AspTrp: 0.991 ± 0.343
2.613AspTyr: 2.613 ± 0.526
0.0AspXaa: 0.0 ± 0.0
Glu
6.217GluAla: 6.217 ± 0.871
0.901GluCys: 0.901 ± 0.353
3.424GluAsp: 3.424 ± 0.55
3.064GluGlu: 3.064 ± 0.665
3.154GluPhe: 3.154 ± 0.453
3.334GluGly: 3.334 ± 0.566
1.171GluHis: 1.171 ± 0.396
4.686GluIle: 4.686 ± 0.766
4.595GluLys: 4.595 ± 0.902
7.299GluLeu: 7.299 ± 0.915
2.343GluMet: 2.343 ± 0.493
3.604GluAsn: 3.604 ± 0.704
2.433GluPro: 2.433 ± 0.478
2.883GluGln: 2.883 ± 0.549
3.064GluArg: 3.064 ± 0.657
5.136GluSer: 5.136 ± 0.791
3.334GluThr: 3.334 ± 0.518
3.875GluVal: 3.875 ± 0.687
1.352GluTrp: 1.352 ± 0.378
3.334GluTyr: 3.334 ± 0.459
0.0GluXaa: 0.0 ± 0.0
Phe
2.343PheAla: 2.343 ± 0.407
0.451PheCys: 0.451 ± 0.196
3.604PheAsp: 3.604 ± 0.517
3.604PheGlu: 3.604 ± 0.71
1.892PhePhe: 1.892 ± 0.452
3.244PheGly: 3.244 ± 0.76
0.541PheHis: 0.541 ± 0.249
2.072PheIle: 2.072 ± 0.525
3.244PheLys: 3.244 ± 0.604
1.982PheLeu: 1.982 ± 0.348
0.991PheMet: 0.991 ± 0.355
2.343PheAsn: 2.343 ± 0.419
1.171PhePro: 1.171 ± 0.379
0.991PheGln: 0.991 ± 0.291
1.712PheArg: 1.712 ± 0.352
2.974PheSer: 2.974 ± 0.603
3.334PheThr: 3.334 ± 0.451
2.433PheVal: 2.433 ± 0.426
0.27PheTrp: 0.27 ± 0.152
1.171PheTyr: 1.171 ± 0.381
0.0PheXaa: 0.0 ± 0.0
Gly
5.316GlyAla: 5.316 ± 0.858
0.991GlyCys: 0.991 ± 0.342
4.866GlyAsp: 4.866 ± 0.934
3.965GlyGlu: 3.965 ± 0.457
4.505GlyPhe: 4.505 ± 0.504
6.217GlyGly: 6.217 ± 0.775
0.811GlyHis: 0.811 ± 0.338
3.064GlyIle: 3.064 ± 0.603
4.956GlyLys: 4.956 ± 0.799
6.578GlyLeu: 6.578 ± 0.807
2.343GlyMet: 2.343 ± 0.469
2.523GlyAsn: 2.523 ± 0.45
0.991GlyPro: 0.991 ± 0.259
2.974GlyGln: 2.974 ± 0.454
1.892GlyArg: 1.892 ± 0.431
4.866GlySer: 4.866 ± 0.775
3.694GlyThr: 3.694 ± 0.844
5.226GlyVal: 5.226 ± 0.558
1.261GlyTrp: 1.261 ± 0.317
3.244GlyTyr: 3.244 ± 0.631
0.0GlyXaa: 0.0 ± 0.0
His
0.901HisAla: 0.901 ± 0.314
0.541HisCys: 0.541 ± 0.175
0.721HisAsp: 0.721 ± 0.262
0.991HisGlu: 0.991 ± 0.315
0.811HisPhe: 0.811 ± 0.297
1.081HisGly: 1.081 ± 0.305
0.451HisHis: 0.451 ± 0.225
1.622HisIle: 1.622 ± 0.344
0.541HisLys: 0.541 ± 0.211
2.253HisLeu: 2.253 ± 0.458
0.451HisMet: 0.451 ± 0.201
0.901HisAsn: 0.901 ± 0.315
0.721HisPro: 0.721 ± 0.282
0.451HisGln: 0.451 ± 0.2
1.081HisArg: 1.081 ± 0.302
1.261HisSer: 1.261 ± 0.424
0.631HisThr: 0.631 ± 0.25
0.811HisVal: 0.811 ± 0.29
0.09HisTrp: 0.09 ± 0.094
1.081HisTyr: 1.081 ± 0.271
0.0HisXaa: 0.0 ± 0.0
Ile
5.767IleAla: 5.767 ± 0.721
0.991IleCys: 0.991 ± 0.334
5.226IleAsp: 5.226 ± 0.67
5.226IleGlu: 5.226 ± 0.783
1.532IlePhe: 1.532 ± 0.319
4.595IleGly: 4.595 ± 0.466
0.901IleHis: 0.901 ± 0.341
2.523IleIle: 2.523 ± 0.438
5.406IleLys: 5.406 ± 0.903
3.244IleLeu: 3.244 ± 0.511
1.442IleMet: 1.442 ± 0.327
3.244IleAsn: 3.244 ± 0.478
3.064IlePro: 3.064 ± 0.511
1.892IleGln: 1.892 ± 0.272
2.163IleArg: 2.163 ± 0.317
2.793IleSer: 2.793 ± 0.522
4.055IleThr: 4.055 ± 0.462
2.433IleVal: 2.433 ± 0.428
0.541IleTrp: 0.541 ± 0.309
2.253IleTyr: 2.253 ± 0.512
0.0IleXaa: 0.0 ± 0.0
Lys
5.226LysAla: 5.226 ± 0.667
0.901LysCys: 0.901 ± 0.323
3.154LysAsp: 3.154 ± 0.703
5.496LysGlu: 5.496 ± 1.226
2.163LysPhe: 2.163 ± 0.405
3.694LysGly: 3.694 ± 0.465
1.442LysHis: 1.442 ± 0.413
3.514LysIle: 3.514 ± 0.561
5.226LysLys: 5.226 ± 0.799
6.037LysLeu: 6.037 ± 0.805
2.703LysMet: 2.703 ± 0.492
3.244LysAsn: 3.244 ± 0.52
2.703LysPro: 2.703 ± 0.547
2.883LysGln: 2.883 ± 0.462
3.334LysArg: 3.334 ± 0.593
4.055LysSer: 4.055 ± 0.726
3.875LysThr: 3.875 ± 0.487
5.677LysVal: 5.677 ± 0.85
1.261LysTrp: 1.261 ± 0.256
2.343LysTyr: 2.343 ± 0.548
0.09LysXaa: 0.09 ± 0.096
Leu
7.118LeuAla: 7.118 ± 1.194
1.532LeuCys: 1.532 ± 0.471
4.686LeuAsp: 4.686 ± 0.686
4.956LeuGlu: 4.956 ± 0.797
1.982LeuPhe: 1.982 ± 0.414
6.668LeuGly: 6.668 ± 1.041
1.892LeuHis: 1.892 ± 0.388
4.145LeuIle: 4.145 ± 0.678
4.145LeuLys: 4.145 ± 0.644
5.136LeuLeu: 5.136 ± 0.608
1.622LeuMet: 1.622 ± 0.433
5.587LeuAsn: 5.587 ± 0.791
3.154LeuPro: 3.154 ± 0.596
2.883LeuGln: 2.883 ± 0.462
3.965LeuArg: 3.965 ± 0.511
5.767LeuSer: 5.767 ± 0.857
5.046LeuThr: 5.046 ± 0.753
5.947LeuVal: 5.947 ± 0.696
1.081LeuTrp: 1.081 ± 0.221
2.163LeuTyr: 2.163 ± 0.427
0.0LeuXaa: 0.0 ± 0.0
Met
3.514MetAla: 3.514 ± 0.589
0.451MetCys: 0.451 ± 0.196
1.352MetAsp: 1.352 ± 0.331
1.081MetGlu: 1.081 ± 0.319
1.532MetPhe: 1.532 ± 0.292
1.261MetGly: 1.261 ± 0.369
0.27MetHis: 0.27 ± 0.155
1.802MetIle: 1.802 ± 0.342
1.802MetLys: 1.802 ± 0.52
2.072MetLeu: 2.072 ± 0.389
0.811MetMet: 0.811 ± 0.26
1.352MetAsn: 1.352 ± 0.389
0.721MetPro: 0.721 ± 0.223
0.991MetGln: 0.991 ± 0.323
1.802MetArg: 1.802 ± 0.461
3.694MetSer: 3.694 ± 0.505
1.892MetThr: 1.892 ± 0.472
1.352MetVal: 1.352 ± 0.362
0.09MetTrp: 0.09 ± 0.093
0.901MetTyr: 0.901 ± 0.335
0.0MetXaa: 0.0 ± 0.0
Asn
3.334AsnAla: 3.334 ± 0.529
0.451AsnCys: 0.451 ± 0.181
3.604AsnAsp: 3.604 ± 0.504
4.235AsnGlu: 4.235 ± 0.588
1.532AsnPhe: 1.532 ± 0.394
4.776AsnGly: 4.776 ± 0.632
1.171AsnHis: 1.171 ± 0.232
2.163AsnIle: 2.163 ± 0.482
3.694AsnLys: 3.694 ± 0.545
3.514AsnLeu: 3.514 ± 0.501
0.541AsnMet: 0.541 ± 0.245
3.784AsnAsn: 3.784 ± 0.536
3.064AsnPro: 3.064 ± 0.554
2.883AsnGln: 2.883 ± 0.736
1.982AsnArg: 1.982 ± 0.369
2.793AsnSer: 2.793 ± 0.591
3.334AsnThr: 3.334 ± 0.673
3.244AsnVal: 3.244 ± 0.689
0.901AsnTrp: 0.901 ± 0.273
1.802AsnTyr: 1.802 ± 0.316
0.0AsnXaa: 0.0 ± 0.0
Pro
1.802ProAla: 1.802 ± 0.345
0.451ProCys: 0.451 ± 0.19
2.072ProAsp: 2.072 ± 0.327
3.694ProGlu: 3.694 ± 0.567
1.802ProPhe: 1.802 ± 0.435
1.352ProGly: 1.352 ± 0.345
0.451ProHis: 0.451 ± 0.18
1.712ProIle: 1.712 ± 0.455
2.523ProLys: 2.523 ± 0.515
1.622ProLeu: 1.622 ± 0.43
1.261ProMet: 1.261 ± 0.333
1.352ProAsn: 1.352 ± 0.343
1.171ProPro: 1.171 ± 0.346
1.171ProGln: 1.171 ± 0.384
1.802ProArg: 1.802 ± 0.356
2.433ProSer: 2.433 ± 0.476
3.604ProThr: 3.604 ± 0.524
3.154ProVal: 3.154 ± 0.601
0.27ProTrp: 0.27 ± 0.152
1.892ProTyr: 1.892 ± 0.356
0.0ProXaa: 0.0 ± 0.0
Gln
3.604GlnAla: 3.604 ± 0.864
0.541GlnCys: 0.541 ± 0.228
2.433GlnAsp: 2.433 ± 0.378
1.982GlnGlu: 1.982 ± 0.613
1.532GlnPhe: 1.532 ± 0.361
2.163GlnGly: 2.163 ± 0.434
0.541GlnHis: 0.541 ± 0.254
3.334GlnIle: 3.334 ± 0.57
1.892GlnLys: 1.892 ± 0.399
3.604GlnLeu: 3.604 ± 0.673
1.081GlnMet: 1.081 ± 0.264
1.442GlnAsn: 1.442 ± 0.386
1.261GlnPro: 1.261 ± 0.302
2.253GlnGln: 2.253 ± 0.397
1.171GlnArg: 1.171 ± 0.254
2.072GlnSer: 2.072 ± 0.447
1.622GlnThr: 1.622 ± 0.306
3.334GlnVal: 3.334 ± 0.629
0.451GlnTrp: 0.451 ± 0.191
0.901GlnTyr: 0.901 ± 0.308
0.0GlnXaa: 0.0 ± 0.0
Arg
3.694ArgAla: 3.694 ± 0.639
1.081ArgCys: 1.081 ± 0.33
1.982ArgAsp: 1.982 ± 0.487
2.523ArgGlu: 2.523 ± 0.468
1.352ArgPhe: 1.352 ± 0.347
1.892ArgGly: 1.892 ± 0.382
1.081ArgHis: 1.081 ± 0.427
2.253ArgIle: 2.253 ± 0.5
2.523ArgLys: 2.523 ± 0.492
3.694ArgLeu: 3.694 ± 0.608
2.072ArgMet: 2.072 ± 0.503
1.802ArgAsn: 1.802 ± 0.268
1.081ArgPro: 1.081 ± 0.319
1.261ArgGln: 1.261 ± 0.404
2.072ArgArg: 2.072 ± 0.403
3.064ArgSer: 3.064 ± 0.552
1.622ArgThr: 1.622 ± 0.278
3.334ArgVal: 3.334 ± 0.634
0.36ArgTrp: 0.36 ± 0.151
1.081ArgTyr: 1.081 ± 0.275
0.0ArgXaa: 0.0 ± 0.0
Ser
4.415SerAla: 4.415 ± 0.718
1.171SerCys: 1.171 ± 0.357
4.055SerAsp: 4.055 ± 0.523
3.784SerGlu: 3.784 ± 0.736
2.974SerPhe: 2.974 ± 0.447
6.037SerGly: 6.037 ± 1.192
1.261SerHis: 1.261 ± 0.36
5.046SerIle: 5.046 ± 0.64
5.316SerLys: 5.316 ± 0.95
6.127SerLeu: 6.127 ± 0.824
2.072SerMet: 2.072 ± 0.439
3.424SerAsn: 3.424 ± 0.598
2.072SerPro: 2.072 ± 0.349
3.244SerGln: 3.244 ± 0.491
2.343SerArg: 2.343 ± 0.399
5.677SerSer: 5.677 ± 1.251
3.334SerThr: 3.334 ± 0.769
4.145SerVal: 4.145 ± 0.576
0.901SerTrp: 0.901 ± 0.338
2.253SerTyr: 2.253 ± 0.564
0.0SerXaa: 0.0 ± 0.0
Thr
5.947ThrAla: 5.947 ± 0.83
0.541ThrCys: 0.541 ± 0.199
3.334ThrAsp: 3.334 ± 0.406
4.505ThrGlu: 4.505 ± 0.531
2.703ThrPhe: 2.703 ± 0.551
4.776ThrGly: 4.776 ± 0.67
0.631ThrHis: 0.631 ± 0.311
3.064ThrIle: 3.064 ± 0.507
2.974ThrLys: 2.974 ± 0.571
4.325ThrLeu: 4.325 ± 0.78
1.352ThrMet: 1.352 ± 0.412
3.154ThrAsn: 3.154 ± 0.438
3.334ThrPro: 3.334 ± 0.688
1.081ThrGln: 1.081 ± 0.337
0.811ThrArg: 0.811 ± 0.272
5.136ThrSer: 5.136 ± 0.989
2.703ThrThr: 2.703 ± 0.73
4.415ThrVal: 4.415 ± 0.697
0.541ThrTrp: 0.541 ± 0.252
2.072ThrTyr: 2.072 ± 0.629
0.0ThrXaa: 0.0 ± 0.0
Val
4.776ValAla: 4.776 ± 0.784
1.261ValCys: 1.261 ± 0.421
5.136ValAsp: 5.136 ± 0.581
4.505ValGlu: 4.505 ± 0.697
2.613ValPhe: 2.613 ± 0.484
4.595ValGly: 4.595 ± 0.635
0.36ValHis: 0.36 ± 0.194
4.325ValIle: 4.325 ± 0.802
4.776ValLys: 4.776 ± 0.544
4.055ValLeu: 4.055 ± 0.64
1.982ValMet: 1.982 ± 0.398
5.136ValAsn: 5.136 ± 0.687
1.622ValPro: 1.622 ± 0.349
1.982ValGln: 1.982 ± 0.502
2.253ValArg: 2.253 ± 0.485
5.947ValSer: 5.947 ± 0.81
4.235ValThr: 4.235 ± 0.573
4.415ValVal: 4.415 ± 0.51
0.36ValTrp: 0.36 ± 0.185
1.892ValTyr: 1.892 ± 0.479
0.0ValXaa: 0.0 ± 0.0
Trp
0.721TrpAla: 0.721 ± 0.277
0.27TrpCys: 0.27 ± 0.144
0.721TrpAsp: 0.721 ± 0.237
1.081TrpGlu: 1.081 ± 0.338
0.721TrpPhe: 0.721 ± 0.312
0.27TrpGly: 0.27 ± 0.14
0.36TrpHis: 0.36 ± 0.189
0.991TrpIle: 0.991 ± 0.273
0.721TrpLys: 0.721 ± 0.327
1.712TrpLeu: 1.712 ± 0.561
0.27TrpMet: 0.27 ± 0.141
0.721TrpAsn: 0.721 ± 0.257
0.27TrpPro: 0.27 ± 0.148
0.36TrpGln: 0.36 ± 0.184
0.631TrpArg: 0.631 ± 0.169
0.991TrpSer: 0.991 ± 0.311
0.36TrpThr: 0.36 ± 0.162
0.541TrpVal: 0.541 ± 0.248
0.0TrpTrp: 0.0 ± 0.0
0.18TrpTyr: 0.18 ± 0.133
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.154TyrAla: 3.154 ± 0.436
0.631TyrCys: 0.631 ± 0.216
2.523TyrAsp: 2.523 ± 0.47
2.343TyrGlu: 2.343 ± 0.558
1.442TyrPhe: 1.442 ± 0.28
2.793TyrGly: 2.793 ± 0.641
0.721TyrHis: 0.721 ± 0.241
2.253TyrIle: 2.253 ± 0.529
2.703TyrLys: 2.703 ± 0.383
2.703TyrLeu: 2.703 ± 0.453
0.631TyrMet: 0.631 ± 0.243
1.802TyrAsn: 1.802 ± 0.469
1.352TyrPro: 1.352 ± 0.447
1.442TyrGln: 1.442 ± 0.351
1.712TyrArg: 1.712 ± 0.433
2.523TyrSer: 2.523 ± 0.497
1.802TyrThr: 1.802 ± 0.39
1.892TyrVal: 1.892 ± 0.562
0.36TyrTrp: 0.36 ± 0.211
2.163TyrTyr: 2.163 ± 0.453
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.09XaaGlu: 0.09 ± 0.096
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.09XaaLys: 0.09 ± 0.096
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (11099 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski