Magnetofaba australis IT-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Magnetococcales; Magnetococcaceae; Magnetofaba; Magnetofaba australis

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4001 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y2K3M3|A0A1Y2K3M3_9PROT Uncharacterized protein OS=Magnetofaba australis IT-1 OX=1434232 GN=MAIT1_02676 PE=4 SV=1
MM1 pKa = 7.45EE2 pKa = 5.98SIAIAYY8 pKa = 9.34DD9 pKa = 3.78EE10 pKa = 4.61LQSACVLSATDD21 pKa = 3.97EE22 pKa = 4.62EE23 pKa = 4.84IEE25 pKa = 4.21QARR28 pKa = 11.84QSLMANGWSAGPVVALNLDD47 pKa = 3.92GDD49 pKa = 4.37LHH51 pKa = 7.38ALNQPALLSATRR63 pKa = 11.84ILANDD68 pKa = 3.87FDD70 pKa = 5.36YY71 pKa = 11.3DD72 pKa = 3.75GEE74 pKa = 4.48YY75 pKa = 10.8DD76 pKa = 3.91VPLLIIGYY84 pKa = 8.73EE85 pKa = 3.88EE86 pKa = 5.02AYY88 pKa = 10.5NLCRR92 pKa = 11.84DD93 pKa = 4.63AGMSWDD99 pKa = 4.22GFLALPTEE107 pKa = 4.71GQAKK111 pKa = 9.52ILEE114 pKa = 4.38AAGFVSCAEE123 pKa = 4.24ALRR126 pKa = 11.84AAA128 pKa = 4.42

Molecular weight: 13.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y2K004|A0A1Y2K004_9PROT Mrr_cat domain-containing protein OS=Magnetofaba australis IT-1 OX=1434232 GN=MAIT1_05447 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIRR11 pKa = 11.84RR12 pKa = 11.84SRR14 pKa = 11.84THH16 pKa = 6.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 8.96VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.2KK41 pKa = 10.75LIPP44 pKa = 4.09

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4001

0

4001

1356533

23

4328

339.0

37.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.841 ± 0.061

0.929 ± 0.015

5.638 ± 0.043

6.362 ± 0.053

3.455 ± 0.027

7.786 ± 0.046

2.445 ± 0.022

4.879 ± 0.03

3.545 ± 0.035

10.743 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.687 ± 0.019

2.947 ± 0.022

4.99 ± 0.034

4.482 ± 0.043

6.92 ± 0.039

5.387 ± 0.033

4.694 ± 0.037

6.603 ± 0.034

1.41 ± 0.019

2.258 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski