Ralstonia phage RsoP1IDN

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Okabevirinae; Higashivirus; Ralstonia virus RsoP1IDN

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P0VPG1|A0A2P0VPG1_9CAUD DNA-directed RNA polymerase OS=Ralstonia phage RsoP1IDN OX=2060091 GN=RsoP1IDN_22 PE=3 SV=1
MM1 pKa = 5.92THH3 pKa = 6.79KK4 pKa = 10.53IEE6 pKa = 3.72WEE8 pKa = 3.97YY9 pKa = 11.3RR10 pKa = 11.84GVFSMVTASADD21 pKa = 3.6GEE23 pKa = 4.77TVSQAIVGDD32 pKa = 4.16FNLEE36 pKa = 3.78ANAAAVLAAEE46 pKa = 4.39KK47 pKa = 9.89TVTDD51 pKa = 4.58LLSTILSTKK60 pKa = 9.08DD61 pKa = 3.03TNDD64 pKa = 3.2RR65 pKa = 11.84PP66 pKa = 3.81

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P0VPG5|A0A2P0VPG5_9CAUD Uncharacterized protein OS=Ralstonia phage RsoP1IDN OX=2060091 GN=RsoP1IDN_20 PE=4 SV=1
MM1 pKa = 7.92ISIRR5 pKa = 11.84QIKK8 pKa = 9.3SALIRR13 pKa = 11.84KK14 pKa = 9.13LGGYY18 pKa = 8.13TKK20 pKa = 9.95MDD22 pKa = 4.05LNYY25 pKa = 10.83AIAQQAAATYY35 pKa = 9.81RR36 pKa = 11.84KK37 pKa = 8.35TSDD40 pKa = 3.17VYY42 pKa = 10.21QKK44 pKa = 10.86KK45 pKa = 9.48LARR48 pKa = 11.84IRR50 pKa = 11.84GRR52 pKa = 11.84VAAYY56 pKa = 9.58DD57 pKa = 3.47VRR59 pKa = 11.84DD60 pKa = 3.67DD61 pKa = 3.63FGRR64 pKa = 11.84RR65 pKa = 11.84VRR67 pKa = 11.84VEE69 pKa = 4.02DD70 pKa = 4.01LLAA73 pKa = 5.35

Molecular weight: 8.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

11793

47

1648

287.6

31.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.575 ± 0.676

0.763 ± 0.152

6.351 ± 0.262

5.435 ± 0.4

3.104 ± 0.157

8.327 ± 0.396

1.764 ± 0.24

4.282 ± 0.181

4.63 ± 0.303

8.225 ± 0.34

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.697 ± 0.209

3.68 ± 0.236

4.07 ± 0.238

4.859 ± 0.478

6.275 ± 0.364

5.809 ± 0.411

5.927 ± 0.323

6.623 ± 0.279

1.628 ± 0.142

2.976 ± 0.233
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski