Amino acid dipepetide frequency for Ralstonia phage RsoP1IDN

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.098AlaAla: 20.098 ± 2.46
0.763AlaCys: 0.763 ± 0.264
8.565AlaAsp: 8.565 ± 1.198
5.767AlaGlu: 5.767 ± 0.692
2.629AlaPhe: 2.629 ± 0.485
11.957AlaGly: 11.957 ± 1.36
1.95AlaHis: 1.95 ± 0.546
4.071AlaIle: 4.071 ± 0.55
6.275AlaLys: 6.275 ± 0.882
10.855AlaLeu: 10.855 ± 1.187
4.41AlaMet: 4.41 ± 0.592
5.512AlaAsn: 5.512 ± 1.153
2.799AlaPro: 2.799 ± 0.408
6.615AlaGln: 6.615 ± 1.202
7.547AlaArg: 7.547 ± 0.877
6.106AlaSer: 6.106 ± 0.758
5.936AlaThr: 5.936 ± 0.721
8.396AlaVal: 8.396 ± 0.954
1.611AlaTrp: 1.611 ± 0.418
3.901AlaTyr: 3.901 ± 0.691
0.0AlaXaa: 0.0 ± 0.0
Cys
0.763CysAla: 0.763 ± 0.297
0.0CysCys: 0.0 ± 0.0
0.678CysAsp: 0.678 ± 0.307
0.254CysGlu: 0.254 ± 0.139
0.254CysPhe: 0.254 ± 0.129
0.678CysGly: 0.678 ± 0.297
0.339CysHis: 0.339 ± 0.212
0.254CysIle: 0.254 ± 0.187
0.254CysLys: 0.254 ± 0.175
0.763CysLeu: 0.763 ± 0.26
0.339CysMet: 0.339 ± 0.198
0.254CysAsn: 0.254 ± 0.157
0.254CysPro: 0.254 ± 0.156
0.509CysGln: 0.509 ± 0.237
0.509CysArg: 0.509 ± 0.225
0.424CysSer: 0.424 ± 0.22
0.339CysThr: 0.339 ± 0.164
0.678CysVal: 0.678 ± 0.305
0.085CysTrp: 0.085 ± 0.082
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
8.735AspAla: 8.735 ± 1.217
0.594AspCys: 0.594 ± 0.211
4.24AspAsp: 4.24 ± 0.878
3.731AspGlu: 3.731 ± 0.643
2.629AspPhe: 2.629 ± 0.444
4.919AspGly: 4.919 ± 0.712
0.678AspHis: 0.678 ± 0.215
2.799AspIle: 2.799 ± 0.42
2.629AspLys: 2.629 ± 0.458
6.106AspLeu: 6.106 ± 1.039
2.12AspMet: 2.12 ± 0.35
2.035AspAsn: 2.035 ± 0.467
4.071AspPro: 4.071 ± 0.51
2.035AspGln: 2.035 ± 0.455
2.883AspArg: 2.883 ± 0.504
2.12AspSer: 2.12 ± 0.416
3.392AspThr: 3.392 ± 0.562
4.834AspVal: 4.834 ± 0.493
0.763AspTrp: 0.763 ± 0.281
2.205AspTyr: 2.205 ± 0.331
0.0AspXaa: 0.0 ± 0.0
Glu
6.784GluAla: 6.784 ± 0.893
0.678GluCys: 0.678 ± 0.268
3.392GluAsp: 3.392 ± 0.696
3.307GluGlu: 3.307 ± 0.66
2.035GluPhe: 2.035 ± 0.434
3.392GluGly: 3.392 ± 0.676
1.357GluHis: 1.357 ± 0.283
2.29GluIle: 2.29 ± 0.483
2.714GluLys: 2.714 ± 0.563
4.579GluLeu: 4.579 ± 0.605
2.205GluMet: 2.205 ± 0.538
1.781GluAsn: 1.781 ± 0.383
1.442GluPro: 1.442 ± 0.324
3.477GluGln: 3.477 ± 0.445
3.307GluArg: 3.307 ± 0.605
2.12GluSer: 2.12 ± 0.329
2.374GluThr: 2.374 ± 0.334
3.477GluVal: 3.477 ± 0.671
1.611GluTrp: 1.611 ± 0.462
1.95GluTyr: 1.95 ± 0.372
0.0GluXaa: 0.0 ± 0.0
Phe
3.138PheAla: 3.138 ± 0.619
0.17PheCys: 0.17 ± 0.114
2.799PheAsp: 2.799 ± 0.554
2.29PheGlu: 2.29 ± 0.465
0.678PhePhe: 0.678 ± 0.238
3.562PheGly: 3.562 ± 0.491
0.509PheHis: 0.509 ± 0.176
1.526PheIle: 1.526 ± 0.413
1.102PheLys: 1.102 ± 0.272
3.053PheLeu: 3.053 ± 0.599
0.424PheMet: 0.424 ± 0.15
1.272PheAsn: 1.272 ± 0.342
0.509PhePro: 0.509 ± 0.255
1.95PheGln: 1.95 ± 0.366
1.187PheArg: 1.187 ± 0.247
1.696PheSer: 1.696 ± 0.326
1.781PheThr: 1.781 ± 0.374
1.781PheVal: 1.781 ± 0.425
0.594PheTrp: 0.594 ± 0.242
1.018PheTyr: 1.018 ± 0.243
0.0PheXaa: 0.0 ± 0.0
Gly
9.244GlyAla: 9.244 ± 1.218
0.678GlyCys: 0.678 ± 0.27
5.597GlyAsp: 5.597 ± 0.702
3.986GlyGlu: 3.986 ± 0.67
2.714GlyPhe: 2.714 ± 0.513
8.226GlyGly: 8.226 ± 0.834
1.187GlyHis: 1.187 ± 0.367
3.647GlyIle: 3.647 ± 0.526
4.579GlyLys: 4.579 ± 0.673
5.258GlyLeu: 5.258 ± 0.919
2.29GlyMet: 2.29 ± 0.55
3.053GlyAsn: 3.053 ± 0.604
2.12GlyPro: 2.12 ± 0.573
3.986GlyGln: 3.986 ± 0.743
6.53GlyArg: 6.53 ± 0.834
5.258GlySer: 5.258 ± 0.765
5.682GlyThr: 5.682 ± 0.737
5.427GlyVal: 5.427 ± 0.526
1.187GlyTrp: 1.187 ± 0.394
2.629GlyTyr: 2.629 ± 0.533
0.0GlyXaa: 0.0 ± 0.0
His
1.696HisAla: 1.696 ± 0.584
0.339HisCys: 0.339 ± 0.149
1.018HisAsp: 1.018 ± 0.309
1.102HisGlu: 1.102 ± 0.407
0.509HisPhe: 0.509 ± 0.22
1.442HisGly: 1.442 ± 0.345
0.339HisHis: 0.339 ± 0.18
0.848HisIle: 0.848 ± 0.28
1.102HisLys: 1.102 ± 0.392
1.526HisLeu: 1.526 ± 0.381
0.424HisMet: 0.424 ± 0.25
0.594HisAsn: 0.594 ± 0.289
0.848HisPro: 0.848 ± 0.406
0.509HisGln: 0.509 ± 0.215
0.933HisArg: 0.933 ± 0.234
0.848HisSer: 0.848 ± 0.334
1.018HisThr: 1.018 ± 0.33
1.442HisVal: 1.442 ± 0.335
0.339HisTrp: 0.339 ± 0.154
0.763HisTyr: 0.763 ± 0.249
0.0HisXaa: 0.0 ± 0.0
Ile
4.664IleAla: 4.664 ± 0.565
0.254IleCys: 0.254 ± 0.145
2.883IleAsp: 2.883 ± 0.569
3.392IleGlu: 3.392 ± 0.583
1.272IlePhe: 1.272 ± 0.341
3.223IleGly: 3.223 ± 0.404
0.848IleHis: 0.848 ± 0.218
1.866IleIle: 1.866 ± 0.326
2.629IleLys: 2.629 ± 0.492
2.29IleLeu: 2.29 ± 0.35
0.933IleMet: 0.933 ± 0.29
1.526IleAsn: 1.526 ± 0.428
1.95IlePro: 1.95 ± 0.437
1.781IleGln: 1.781 ± 0.399
2.714IleArg: 2.714 ± 0.521
2.714IleSer: 2.714 ± 0.494
2.799IleThr: 2.799 ± 0.539
3.647IleVal: 3.647 ± 0.554
0.339IleTrp: 0.339 ± 0.199
1.102IleTyr: 1.102 ± 0.375
0.0IleXaa: 0.0 ± 0.0
Lys
6.021LysAla: 6.021 ± 0.797
0.424LysCys: 0.424 ± 0.231
2.459LysAsp: 2.459 ± 0.446
2.714LysGlu: 2.714 ± 0.527
1.526LysPhe: 1.526 ± 0.384
2.12LysGly: 2.12 ± 0.589
0.933LysHis: 0.933 ± 0.33
1.866LysIle: 1.866 ± 0.431
2.459LysLys: 2.459 ± 0.492
5.088LysLeu: 5.088 ± 0.859
1.018LysMet: 1.018 ± 0.268
1.526LysAsn: 1.526 ± 0.357
1.611LysPro: 1.611 ± 0.479
2.035LysGln: 2.035 ± 0.435
3.138LysArg: 3.138 ± 0.537
3.477LysSer: 3.477 ± 0.621
3.053LysThr: 3.053 ± 0.547
2.714LysVal: 2.714 ± 0.42
0.678LysTrp: 0.678 ± 0.194
1.442LysTyr: 1.442 ± 0.317
0.0LysXaa: 0.0 ± 0.0
Leu
9.498LeuAla: 9.498 ± 0.78
0.594LeuCys: 0.594 ± 0.229
4.664LeuAsp: 4.664 ± 0.559
4.155LeuGlu: 4.155 ± 0.603
1.866LeuPhe: 1.866 ± 0.44
7.632LeuGly: 7.632 ± 0.833
1.866LeuHis: 1.866 ± 0.44
3.053LeuIle: 3.053 ± 0.469
3.053LeuLys: 3.053 ± 0.577
6.275LeuLeu: 6.275 ± 0.596
1.611LeuMet: 1.611 ± 0.386
2.799LeuAsn: 2.799 ± 0.625
3.562LeuPro: 3.562 ± 0.553
4.41LeuGln: 4.41 ± 0.667
7.208LeuArg: 7.208 ± 1.135
6.36LeuSer: 6.36 ± 0.881
6.445LeuThr: 6.445 ± 0.506
4.919LeuVal: 4.919 ± 0.659
1.018LeuTrp: 1.018 ± 0.219
1.272LeuTyr: 1.272 ± 0.249
0.0LeuXaa: 0.0 ± 0.0
Met
3.477MetAla: 3.477 ± 0.424
0.17MetCys: 0.17 ± 0.132
1.866MetAsp: 1.866 ± 0.374
1.272MetGlu: 1.272 ± 0.394
0.594MetPhe: 0.594 ± 0.255
1.781MetGly: 1.781 ± 0.302
0.678MetHis: 0.678 ± 0.234
1.357MetIle: 1.357 ± 0.357
0.763MetLys: 0.763 ± 0.251
3.053MetLeu: 3.053 ± 0.546
0.339MetMet: 0.339 ± 0.182
1.272MetAsn: 1.272 ± 0.405
1.187MetPro: 1.187 ± 0.447
0.848MetGln: 0.848 ± 0.226
1.781MetArg: 1.781 ± 0.431
1.95MetSer: 1.95 ± 0.331
1.95MetThr: 1.95 ± 0.394
1.272MetVal: 1.272 ± 0.307
0.678MetTrp: 0.678 ± 0.286
0.678MetTyr: 0.678 ± 0.248
0.0MetXaa: 0.0 ± 0.0
Asn
5.343AsnAla: 5.343 ± 0.75
0.17AsnCys: 0.17 ± 0.134
1.442AsnAsp: 1.442 ± 0.299
1.442AsnGlu: 1.442 ± 0.301
1.611AsnPhe: 1.611 ± 0.372
3.138AsnGly: 3.138 ± 0.629
0.17AsnHis: 0.17 ± 0.131
1.866AsnIle: 1.866 ± 0.452
1.357AsnLys: 1.357 ± 0.265
3.477AsnLeu: 3.477 ± 0.609
0.933AsnMet: 0.933 ± 0.297
1.442AsnAsn: 1.442 ± 0.369
2.459AsnPro: 2.459 ± 0.375
1.696AsnGln: 1.696 ± 0.365
1.696AsnArg: 1.696 ± 0.369
2.714AsnSer: 2.714 ± 0.545
2.205AsnThr: 2.205 ± 0.494
2.459AsnVal: 2.459 ± 0.505
0.594AsnTrp: 0.594 ± 0.207
0.594AsnTyr: 0.594 ± 0.212
0.0AsnXaa: 0.0 ± 0.0
Pro
5.343ProAla: 5.343 ± 0.778
0.085ProCys: 0.085 ± 0.084
3.223ProAsp: 3.223 ± 0.532
2.629ProGlu: 2.629 ± 0.554
0.933ProPhe: 0.933 ± 0.228
4.155ProGly: 4.155 ± 0.657
0.848ProHis: 0.848 ± 0.207
1.611ProIle: 1.611 ± 0.433
1.781ProLys: 1.781 ± 0.301
1.95ProLeu: 1.95 ± 0.389
1.018ProMet: 1.018 ± 0.272
1.018ProAsn: 1.018 ± 0.328
1.781ProPro: 1.781 ± 0.436
2.035ProGln: 2.035 ± 0.429
1.611ProArg: 1.611 ± 0.391
3.223ProSer: 3.223 ± 0.805
2.799ProThr: 2.799 ± 0.445
2.544ProVal: 2.544 ± 0.436
0.678ProTrp: 0.678 ± 0.201
1.442ProTyr: 1.442 ± 0.482
0.0ProXaa: 0.0 ± 0.0
Gln
7.463GlnAla: 7.463 ± 1.19
0.678GlnCys: 0.678 ± 0.252
2.374GlnAsp: 2.374 ± 0.445
1.696GlnGlu: 1.696 ± 0.508
1.696GlnPhe: 1.696 ± 0.428
3.223GlnGly: 3.223 ± 0.661
1.102GlnHis: 1.102 ± 0.297
2.629GlnIle: 2.629 ± 0.647
2.544GlnLys: 2.544 ± 0.407
3.901GlnLeu: 3.901 ± 0.56
0.933GlnMet: 0.933 ± 0.396
1.526GlnAsn: 1.526 ± 0.339
1.95GlnPro: 1.95 ± 0.479
3.562GlnGln: 3.562 ± 0.921
3.477GlnArg: 3.477 ± 0.532
2.968GlnSer: 2.968 ± 0.647
1.611GlnThr: 1.611 ± 0.536
2.629GlnVal: 2.629 ± 0.364
0.594GlnTrp: 0.594 ± 0.255
2.035GlnTyr: 2.035 ± 0.418
0.0GlnXaa: 0.0 ± 0.0
Arg
6.784ArgAla: 6.784 ± 0.511
0.509ArgCys: 0.509 ± 0.246
3.307ArgAsp: 3.307 ± 0.54
4.24ArgGlu: 4.24 ± 0.79
2.459ArgPhe: 2.459 ± 0.411
5.512ArgGly: 5.512 ± 0.667
1.187ArgHis: 1.187 ± 0.458
3.647ArgIle: 3.647 ± 0.638
3.731ArgLys: 3.731 ± 0.581
4.579ArgLeu: 4.579 ± 0.737
1.526ArgMet: 1.526 ± 0.465
2.205ArgAsn: 2.205 ± 0.341
2.205ArgPro: 2.205 ± 0.372
1.866ArgGln: 1.866 ± 0.405
3.986ArgArg: 3.986 ± 0.631
2.883ArgSer: 2.883 ± 0.383
3.986ArgThr: 3.986 ± 0.522
4.664ArgVal: 4.664 ± 0.82
1.357ArgTrp: 1.357 ± 0.324
2.12ArgTyr: 2.12 ± 0.495
0.0ArgXaa: 0.0 ± 0.0
Ser
7.717SerAla: 7.717 ± 1.044
0.424SerCys: 0.424 ± 0.247
4.24SerAsp: 4.24 ± 0.561
2.374SerGlu: 2.374 ± 0.516
1.442SerPhe: 1.442 ± 0.354
4.41SerGly: 4.41 ± 0.703
0.848SerHis: 0.848 ± 0.295
3.223SerIle: 3.223 ± 0.415
2.035SerLys: 2.035 ± 0.483
5.427SerLeu: 5.427 ± 0.606
1.357SerMet: 1.357 ± 0.248
2.12SerAsn: 2.12 ± 0.456
3.053SerPro: 3.053 ± 0.624
2.968SerGln: 2.968 ± 0.622
3.562SerArg: 3.562 ± 0.557
2.799SerSer: 2.799 ± 0.627
4.071SerThr: 4.071 ± 0.709
3.053SerVal: 3.053 ± 0.54
1.442SerTrp: 1.442 ± 0.3
1.526SerTyr: 1.526 ± 0.5
0.0SerXaa: 0.0 ± 0.0
Thr
7.547ThrAla: 7.547 ± 0.928
0.424ThrCys: 0.424 ± 0.193
3.223ThrAsp: 3.223 ± 0.46
3.392ThrGlu: 3.392 ± 0.436
2.29ThrPhe: 2.29 ± 0.522
4.919ThrGly: 4.919 ± 0.565
0.424ThrHis: 0.424 ± 0.189
2.374ThrIle: 2.374 ± 0.36
2.799ThrLys: 2.799 ± 0.362
4.325ThrLeu: 4.325 ± 0.505
1.866ThrMet: 1.866 ± 0.409
1.95ThrAsn: 1.95 ± 0.382
3.986ThrPro: 3.986 ± 0.449
3.307ThrGln: 3.307 ± 0.577
2.544ThrArg: 2.544 ± 0.522
3.307ThrSer: 3.307 ± 0.651
3.562ThrThr: 3.562 ± 0.449
4.155ThrVal: 4.155 ± 0.612
0.848ThrTrp: 0.848 ± 0.217
2.035ThrTyr: 2.035 ± 0.479
0.0ThrXaa: 0.0 ± 0.0
Val
6.615ValAla: 6.615 ± 0.657
0.424ValCys: 0.424 ± 0.177
3.986ValAsp: 3.986 ± 0.428
3.731ValGlu: 3.731 ± 0.511
1.526ValPhe: 1.526 ± 0.393
5.003ValGly: 5.003 ± 0.729
1.526ValHis: 1.526 ± 0.466
2.035ValIle: 2.035 ± 0.474
3.053ValLys: 3.053 ± 0.414
5.088ValLeu: 5.088 ± 0.843
1.781ValMet: 1.781 ± 0.377
3.223ValAsn: 3.223 ± 0.626
3.223ValPro: 3.223 ± 0.518
3.223ValGln: 3.223 ± 0.489
4.919ValArg: 4.919 ± 0.579
4.749ValSer: 4.749 ± 0.785
3.392ValThr: 3.392 ± 0.513
5.427ValVal: 5.427 ± 0.803
1.018ValTrp: 1.018 ± 0.27
2.29ValTyr: 2.29 ± 0.532
0.0ValXaa: 0.0 ± 0.0
Trp
1.526TrpAla: 1.526 ± 0.353
0.085TrpCys: 0.085 ± 0.081
1.187TrpAsp: 1.187 ± 0.409
1.102TrpGlu: 1.102 ± 0.291
0.933TrpPhe: 0.933 ± 0.266
0.848TrpGly: 0.848 ± 0.278
0.678TrpHis: 0.678 ± 0.266
0.339TrpIle: 0.339 ± 0.172
0.933TrpLys: 0.933 ± 0.252
1.95TrpLeu: 1.95 ± 0.459
0.848TrpMet: 0.848 ± 0.241
0.594TrpAsn: 0.594 ± 0.241
0.424TrpPro: 0.424 ± 0.18
0.339TrpGln: 0.339 ± 0.141
1.272TrpArg: 1.272 ± 0.397
0.594TrpSer: 0.594 ± 0.228
0.933TrpThr: 0.933 ± 0.268
1.187TrpVal: 1.187 ± 0.376
0.339TrpTrp: 0.339 ± 0.189
0.17TrpTyr: 0.17 ± 0.126
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.307TyrAla: 3.307 ± 0.592
0.17TyrCys: 0.17 ± 0.141
2.374TyrAsp: 2.374 ± 0.519
1.781TyrGlu: 1.781 ± 0.374
1.442TyrPhe: 1.442 ± 0.297
3.138TyrGly: 3.138 ± 0.369
0.17TyrHis: 0.17 ± 0.109
1.526TyrIle: 1.526 ± 0.446
0.509TyrLys: 0.509 ± 0.203
2.714TyrLeu: 2.714 ± 0.603
0.509TyrMet: 0.509 ± 0.172
1.102TyrAsn: 1.102 ± 0.255
1.272TyrPro: 1.272 ± 0.358
1.442TyrGln: 1.442 ± 0.407
1.95TyrArg: 1.95 ± 0.423
1.781TyrSer: 1.781 ± 0.395
1.95TyrThr: 1.95 ± 0.414
1.526TyrVal: 1.526 ± 0.379
0.509TyrTrp: 0.509 ± 0.273
0.594TyrTyr: 0.594 ± 0.157
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 41 proteins (11793 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski