Blautia argi
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2737 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z4U801|A0A2Z4U801_9FIRM Uncharacterized protein OS=Blautia argi OX=1912897 GN=DQQ01_01245 PE=4 SV=1
MM1 pKa = 7.34 KK2 pKa = 10.24 FIYY5 pKa = 9.69 PAVFRR10 pKa = 11.84 KK11 pKa = 8.58 TEE13 pKa = 4.17 DD14 pKa = 3.13 GTYY17 pKa = 10.13 EE18 pKa = 3.85 GHH20 pKa = 7.0 FPDD23 pKa = 6.15 LEE25 pKa = 4.08 CCYY28 pKa = 11.18 AKK30 pKa = 10.84 GDD32 pKa = 4.03 TLEE35 pKa = 4.79 DD36 pKa = 3.47 AVDD39 pKa = 3.8 NANEE43 pKa = 3.81 AAYY46 pKa = 10.41 NWLSLEE52 pKa = 4.9 LSEE55 pKa = 5.15 EE56 pKa = 4.27 DD57 pKa = 3.9 CNLPSVSDD65 pKa = 3.88 EE66 pKa = 4.65 SDD68 pKa = 2.89 MDD70 pKa = 3.97 LQDD73 pKa = 3.46 GDD75 pKa = 3.97 VVRR78 pKa = 11.84 NISVNIRR85 pKa = 11.84 FYY87 pKa = 11.03 EE88 pKa = 4.51 GWDD91 pKa = 3.35 EE92 pKa = 4.06
Molecular weight: 10.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.795
IPC_protein 3.745
Toseland 3.541
ProMoST 3.884
Dawson 3.732
Bjellqvist 3.935
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.668
Nozaki 3.859
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.872
Patrickios 0.896
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.808
Protein with the highest isoelectric point:
>tr|A0A2Z4U7J7|A0A2Z4U7J7_9FIRM RNA-binding S4 domain-containing protein OS=Blautia argi OX=1912897 GN=DQQ01_00250 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 8.72 MTFQPKK8 pKa = 8.95 KK9 pKa = 7.58 RR10 pKa = 11.84 QRR12 pKa = 11.84 SKK14 pKa = 9.17 VHH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSSAGGRR28 pKa = 11.84 KK29 pKa = 8.81 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.1 GRR39 pKa = 11.84 KK40 pKa = 8.83 KK41 pKa = 10.63 LSAA44 pKa = 3.95
Molecular weight: 5.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.125
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.676
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.349
Sillero 12.866
Patrickios 12.398
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2737
0
2737
869576
25
2219
317.7
35.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.134 ± 0.046
1.558 ± 0.018
4.989 ± 0.033
8.434 ± 0.056
4.276 ± 0.032
7.016 ± 0.037
1.738 ± 0.021
7.117 ± 0.042
7.406 ± 0.041
9.308 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.094 ± 0.021
4.2 ± 0.028
3.247 ± 0.026
3.501 ± 0.023
4.379 ± 0.035
5.53 ± 0.034
5.212 ± 0.028
6.703 ± 0.033
0.955 ± 0.015
4.204 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here