Sutterella megalosphaeroides
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2112 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z6IE84|A0A2Z6IE84_9BURK Urocanate reductase OS=Sutterella megalosphaeroides OX=2494234 GN=urdA_3 PE=3 SV=1
MM1 pKa = 7.64 TEE3 pKa = 5.08 LDD5 pKa = 4.85 DD6 pKa = 6.35 PDD8 pKa = 6.44 DD9 pKa = 4.22 EE10 pKa = 6.06 DD11 pKa = 6.11 PYY13 pKa = 11.57 DD14 pKa = 4.06 EE15 pKa = 4.78 EE16 pKa = 5.6 DD17 pKa = 4.09 NRR19 pKa = 11.84 TLDD22 pKa = 3.61 EE23 pKa = 4.79 RR24 pKa = 11.84 MLDD27 pKa = 3.55 AYY29 pKa = 10.3 GSRR32 pKa = 11.84 SEE34 pKa = 4.29 WEE36 pKa = 3.38 ADD38 pKa = 3.28 AAGEE42 pKa = 4.25 TDD44 pKa = 3.82 WYY46 pKa = 10.52 ADD48 pKa = 3.8 PYY50 pKa = 11.28 RR51 pKa = 11.84 KK52 pKa = 9.42 FLEE55 pKa = 4.36 NGCRR59 pKa = 11.84 GILTYY64 pKa = 10.89 EE65 pKa = 4.26 EE66 pKa = 4.17 AVEE69 pKa = 3.83 AHH71 pKa = 6.32 KK72 pKa = 10.33 WSTGIDD78 pKa = 3.64 PDD80 pKa = 4.68 YY81 pKa = 9.42 VTSWLEE87 pKa = 3.49 IDD89 pKa = 3.1 WVKK92 pKa = 10.57 RR93 pKa = 11.84 QRR95 pKa = 11.84 EE96 pKa = 4.15 QIVEE100 pKa = 4.08 CEE102 pKa = 3.88 DD103 pKa = 4.31 FIRR106 pKa = 11.84 AVFGLSLDD114 pKa = 4.22 EE115 pKa = 4.33 YY116 pKa = 10.55 CALYY120 pKa = 10.47 LPYY123 pKa = 10.91 EE124 pKa = 3.96 PMIRR128 pKa = 11.84 LAYY131 pKa = 10.09
Molecular weight: 15.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.935
IPC_protein 3.897
Toseland 3.706
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.783
Rodwell 3.732
Grimsley 3.617
Solomon 3.872
Lehninger 3.821
Nozaki 3.986
DTASelect 4.177
Thurlkill 3.745
EMBOSS 3.795
Sillero 4.012
Patrickios 1.888
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.915
Protein with the highest isoelectric point:
>tr|A0A2Z6I7Q4|A0A2Z6I7Q4_9BURK 50S ribosomal protein L10 OS=Sutterella megalosphaeroides OX=2494234 GN=rplJ PE=3 SV=1
MM1 pKa = 6.85 ATKK4 pKa = 9.51 RR5 pKa = 11.84 TYY7 pKa = 10.39 QPSKK11 pKa = 8.92 VKK13 pKa = 10.35 RR14 pKa = 11.84 ARR16 pKa = 11.84 THH18 pKa = 5.71 GFLVRR23 pKa = 11.84 SRR25 pKa = 11.84 TKK27 pKa = 9.76 GGRR30 pKa = 11.84 RR31 pKa = 11.84 VLARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 AKK39 pKa = 10.14 GRR41 pKa = 11.84 HH42 pKa = 4.25 VLALL46 pKa = 3.77
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2112
0
2112
763480
40
4012
361.5
39.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.006 ± 0.065
1.08 ± 0.022
5.515 ± 0.044
6.961 ± 0.059
3.867 ± 0.036
8.014 ± 0.059
1.791 ± 0.023
4.364 ± 0.044
4.1 ± 0.055
9.991 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.334 ± 0.027
2.789 ± 0.032
4.859 ± 0.047
2.291 ± 0.025
7.13 ± 0.073
5.576 ± 0.05
5.881 ± 0.051
7.846 ± 0.042
1.254 ± 0.019
2.35 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here