Amycolatopsis suaedae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6878 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q7J5F6|A0A4Q7J5F6_9PSEU Succinate dehydrogenase cytochrome b556 subunit OS=Amycolatopsis suaedae OX=2510978 GN=sdhC PE=4 SV=1
MM1 pKa = 7.53ASSAEE6 pKa = 4.34TASGTPCWAGLTVPDD21 pKa = 4.46RR22 pKa = 11.84EE23 pKa = 4.37LAAGFYY29 pKa = 10.85GPLLGWEE36 pKa = 4.18FGEE39 pKa = 4.46YY40 pKa = 10.32DD41 pKa = 4.81DD42 pKa = 5.69GDD44 pKa = 4.14AVHH47 pKa = 6.78LVCTLGGLPAAGISQGPDD65 pKa = 3.54TLWTTYY71 pKa = 10.82LAVPDD76 pKa = 4.55LAAAAARR83 pKa = 11.84VGPAAGSILVPPRR96 pKa = 11.84DD97 pKa = 3.77VPGGSLAVAVDD108 pKa = 3.58PAGAAVGLWQPAGPPVATGTGAMAWNEE135 pKa = 4.2CLTDD139 pKa = 3.73EE140 pKa = 5.14LDD142 pKa = 3.85AAVTFYY148 pKa = 10.71TGVFGYY154 pKa = 8.2GTIDD158 pKa = 3.15MSVPGFRR165 pKa = 11.84YY166 pKa = 10.09LGLTTGGEE174 pKa = 4.37PVCGLGEE181 pKa = 4.25LPADD185 pKa = 3.81TLGEE189 pKa = 4.44VPAHH193 pKa = 5.33WVTYY197 pKa = 9.36FSVADD202 pKa = 3.4VDD204 pKa = 4.12VATEE208 pKa = 4.03KK209 pKa = 10.78LASLGGRR216 pKa = 11.84MLVPPFDD223 pKa = 4.57DD224 pKa = 4.0AQGSRR229 pKa = 11.84LAVGADD235 pKa = 3.44NQGTMLGLVSPPPSRR250 pKa = 11.84RR251 pKa = 11.84SWW253 pKa = 3.05

Molecular weight: 25.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q7JBY3|A0A4Q7JBY3_9PSEU 30S ribosomal protein S2 OS=Amycolatopsis suaedae OX=2510978 GN=rpsB PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.68LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84MQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.69QGKK33 pKa = 8.53

Molecular weight: 4.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6878

0

6878

2267144

32

7668

329.6

35.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.569 ± 0.046

0.755 ± 0.007

5.984 ± 0.025

5.557 ± 0.028

2.839 ± 0.017

9.539 ± 0.031

2.133 ± 0.014

3.189 ± 0.02

1.918 ± 0.022

10.667 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.656 ± 0.012

1.84 ± 0.015

6.03 ± 0.026

2.679 ± 0.017

8.161 ± 0.031

4.793 ± 0.018

6.027 ± 0.025

9.126 ± 0.029

1.552 ± 0.013

1.985 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski