Candidatus Aquiluna sp. 15G-AUS-rot

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Luna cluster; Luna-1 subcluster; Aquiluna

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1370 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D4U7R3|A0A7D4U7R3_9MICO Tryptophan synthase beta chain OS=Candidatus Aquiluna sp. 15G-AUS-rot OX=2499157 GN=trpB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 10.39LKK4 pKa = 10.39NASAAFALLGASALVLSGCAAAPEE28 pKa = 4.35AEE30 pKa = 4.26PTATEE35 pKa = 4.33SEE37 pKa = 4.63TATEE41 pKa = 3.91EE42 pKa = 3.94AAPSVDD48 pKa = 3.61YY49 pKa = 10.26LACAVSDD56 pKa = 3.51EE57 pKa = 4.7GSWNDD62 pKa = 2.93KK63 pKa = 10.71SFNEE67 pKa = 4.19SVYY70 pKa = 11.22DD71 pKa = 3.8GLLKK75 pKa = 10.78AEE77 pKa = 4.81AEE79 pKa = 4.29LGVQIAEE86 pKa = 4.33AEE88 pKa = 4.5SNSAEE93 pKa = 4.08DD94 pKa = 4.04FAPNLQAMVDD104 pKa = 3.75QACDD108 pKa = 2.77ITFAVGFNLVADD120 pKa = 4.31VNAAAAANPEE130 pKa = 4.38TQFVTIDD137 pKa = 2.97GWSEE141 pKa = 3.61GNANLKK147 pKa = 9.42PVGYY151 pKa = 11.13AMNQSSYY158 pKa = 10.83LAGYY162 pKa = 8.74LAAAYY167 pKa = 7.6STTKK171 pKa = 10.55VVGTYY176 pKa = 10.99GGMQIDD182 pKa = 3.91AVTDD186 pKa = 3.82FMNGFYY192 pKa = 10.18YY193 pKa = 10.86GAMAWGAEE201 pKa = 3.88NGAEE205 pKa = 4.11VKK207 pKa = 10.61VVGWDD212 pKa = 3.28PAAATGQFIGDD223 pKa = 4.06FTPNSGTSKK232 pKa = 10.64SIAAALINDD241 pKa = 4.16GADD244 pKa = 3.07VIFPVGGDD252 pKa = 3.09QFGAVSQAIEE262 pKa = 3.83EE263 pKa = 4.14AGIAGVMIGVDD274 pKa = 3.29KK275 pKa = 11.08DD276 pKa = 3.67VALTSPEE283 pKa = 3.86YY284 pKa = 10.7APYY287 pKa = 11.12VLTSAEE293 pKa = 3.86KK294 pKa = 10.26RR295 pKa = 11.84MTNAVYY301 pKa = 10.45DD302 pKa = 3.96IVAEE306 pKa = 4.35LSAGGEE312 pKa = 4.23FSGDD316 pKa = 3.24AYY318 pKa = 11.22LGTLANGGTGLSPFYY333 pKa = 10.74EE334 pKa = 4.55FDD336 pKa = 3.98SKK338 pKa = 11.17IDD340 pKa = 3.77DD341 pKa = 3.55ATKK344 pKa = 10.74ARR346 pKa = 11.84LAEE349 pKa = 4.19LEE351 pKa = 3.95AGIIAGEE358 pKa = 4.01IDD360 pKa = 3.75PLSS363 pKa = 3.6

Molecular weight: 37.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D4PZX1|A0A7D4PZX1_9MICO Aminobutyraldehyde dehydrogenase OS=Candidatus Aquiluna sp. 15G-AUS-rot OX=2499157 GN=HRU87_04745 PE=3 SV=1
MM1 pKa = 7.46GSIIKK6 pKa = 9.98KK7 pKa = 8.35RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.39RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.31LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1370

0

1370

429841

32

1516

313.8

34.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.844 ± 0.085

0.528 ± 0.014

5.125 ± 0.048

6.574 ± 0.065

3.895 ± 0.044

8.1 ± 0.047

1.776 ± 0.032

5.677 ± 0.045

4.217 ± 0.05

10.785 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.084 ± 0.024

3.103 ± 0.038

4.517 ± 0.034

3.667 ± 0.033

5.506 ± 0.052

6.796 ± 0.052

5.25 ± 0.061

7.996 ± 0.055

1.296 ± 0.027

2.265 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski