Pseudooceanicola atlanticus
Average proteome isoelectric point is 5.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4202 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0EFQ7|A0A0A0EFQ7_9RHOB Protein-tyrosine-phosphatase OS=Pseudooceanicola atlanticus OX=1461694 GN=ATO9_10850 PE=3 SV=1
MM1 pKa = 6.76 YY2 pKa = 10.63 AKK4 pKa = 10.24 ILGLSTVAVLTATSAMATTDD24 pKa = 3.12 ATATTDD30 pKa = 3.38 LNLRR34 pKa = 11.84 SLPDD38 pKa = 3.47 PRR40 pKa = 11.84 GEE42 pKa = 3.95 ILDD45 pKa = 4.27 VIPGEE50 pKa = 3.94 AMVQIEE56 pKa = 4.43 QCTTDD61 pKa = 3.59 TTWCKK66 pKa = 10.64 VNYY69 pKa = 10.12 DD70 pKa = 3.76 GTEE73 pKa = 3.57 GWAYY77 pKa = 10.87 SPYY80 pKa = 9.73 LTASLDD86 pKa = 3.66 EE87 pKa = 4.59 EE88 pKa = 4.15 PVVIYY93 pKa = 10.65 EE94 pKa = 3.88 NVEE97 pKa = 3.89 RR98 pKa = 11.84 FEE100 pKa = 4.28 VEE102 pKa = 4.81 SVDD105 pKa = 5.69 LNDD108 pKa = 5.11 DD109 pKa = 3.53 DD110 pKa = 4.95 RR111 pKa = 11.84 AAAAAAGGLTGGALAVAAAGGPAAIAGAVALGTIAGAEE149 pKa = 4.2 SVPEE153 pKa = 3.8 QTVTYY158 pKa = 9.54 VQSNPLDD165 pKa = 3.56 PVYY168 pKa = 11.43 VNGEE172 pKa = 4.34 VVVGAGIPQEE182 pKa = 4.27 VEE184 pKa = 3.7 LVTIPEE190 pKa = 3.51 SDD192 pKa = 3.35 YY193 pKa = 11.09 RR194 pKa = 11.84 YY195 pKa = 10.43 VYY197 pKa = 11.24 LNGTPVLVDD206 pKa = 3.45 EE207 pKa = 5.35 DD208 pKa = 3.95 RR209 pKa = 11.84 VIVNVVRR216 pKa = 4.15
Molecular weight: 22.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.757
IPC_protein 3.719
Toseland 3.528
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.617
Rodwell 3.554
Grimsley 3.439
Solomon 3.694
Lehninger 3.643
Nozaki 3.821
DTASelect 3.999
Thurlkill 3.567
EMBOSS 3.63
Sillero 3.846
Patrickios 0.693
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.763
Protein with the highest isoelectric point:
>tr|A0A0A0EAI8|A0A0A0EAI8_9RHOB Murein hydrolase A OS=Pseudooceanicola atlanticus OX=1461694 GN=ATO9_19905 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MASKK25 pKa = 10.56 AGRR28 pKa = 11.84 KK29 pKa = 8.71 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4202
0
4202
1327093
41
7143
315.8
34.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.069 ± 0.056
0.844 ± 0.012
6.45 ± 0.048
6.145 ± 0.036
3.672 ± 0.03
8.946 ± 0.054
2.007 ± 0.019
5.257 ± 0.028
3.087 ± 0.031
9.855 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.89 ± 0.023
2.568 ± 0.022
5.054 ± 0.03
3.119 ± 0.02
6.633 ± 0.046
5.061 ± 0.028
5.568 ± 0.03
7.199 ± 0.034
1.339 ± 0.017
2.237 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here