Micromonospora avicenniae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6031 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N7B923|A0A1N7B923_9ACTN Undecaprenyl-diphosphatase OS=Micromonospora avicenniae OX=1198245 GN=SAMN05444858_110192 PE=4 SV=1
MM1 pKa = 7.16TKK3 pKa = 10.05VVYY6 pKa = 9.95AAEE9 pKa = 4.23PAVGWRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84LGAIPVVPVLVSALLPVLVLSSPAAAADD45 pKa = 3.65LNMTVRR51 pKa = 11.84VTVEE55 pKa = 3.61RR56 pKa = 11.84VEE58 pKa = 4.11GHH60 pKa = 7.24DD61 pKa = 3.97FGEE64 pKa = 4.81DD65 pKa = 3.3PDD67 pKa = 4.5FYY69 pKa = 11.26SYY71 pKa = 11.79VEE73 pKa = 3.83IAGEE77 pKa = 4.16SFNNEE82 pKa = 3.97DD83 pKa = 4.32TPASDD88 pKa = 4.02PFEE91 pKa = 4.32DD92 pKa = 4.56TYY94 pKa = 11.14TIEE97 pKa = 4.51PNWEE101 pKa = 3.65FSKK104 pKa = 10.89QVALSSGTVPIYY116 pKa = 10.92LEE118 pKa = 4.09IRR120 pKa = 11.84DD121 pKa = 3.68EE122 pKa = 4.7DD123 pKa = 3.74GFLRR127 pKa = 11.84FDD129 pKa = 5.46DD130 pKa = 5.47DD131 pKa = 5.68FADD134 pKa = 5.01LDD136 pKa = 4.53PDD138 pKa = 4.9GDD140 pKa = 4.72DD141 pKa = 5.09PDD143 pKa = 6.1DD144 pKa = 4.24PTDD147 pKa = 4.05NDD149 pKa = 4.47DD150 pKa = 5.38DD151 pKa = 4.37TDD153 pKa = 3.57QSLSFTVDD161 pKa = 3.45LNACTVSGDD170 pKa = 3.8LNGGCGVFTSTGTPDD185 pKa = 2.97EE186 pKa = 4.39SASIRR191 pKa = 11.84LRR193 pKa = 11.84VEE195 pKa = 3.7VTEE198 pKa = 4.66PPAAPGIRR206 pKa = 11.84IRR208 pKa = 11.84CMHH211 pKa = 6.8TPLFPQVGDD220 pKa = 3.72TVTITAEE227 pKa = 4.07FLDD230 pKa = 4.75GALTPARR237 pKa = 11.84ADD239 pKa = 3.52TLEE242 pKa = 3.46IWVNDD247 pKa = 3.31RR248 pKa = 11.84TAPDD252 pKa = 3.26VSTANSLTLTYY263 pKa = 10.96NHH265 pKa = 7.71TITGGGSFSYY275 pKa = 10.48GCRR278 pKa = 11.84GVDD281 pKa = 3.2DD282 pKa = 4.38SVPIFTGWRR291 pKa = 11.84SVAATAGAGDD301 pKa = 4.55EE302 pKa = 4.57VPVLYY307 pKa = 10.13TGSTYY312 pKa = 11.16SRR314 pKa = 11.84VDD316 pKa = 2.95WVFVGDD322 pKa = 3.71SDD324 pKa = 5.39DD325 pKa = 4.04YY326 pKa = 11.94NGAADD331 pKa = 4.22PQFQSDD337 pKa = 4.13VQQIIYY343 pKa = 10.73NSFYY347 pKa = 10.74SYY349 pKa = 11.61DD350 pKa = 3.79PFLVHH355 pKa = 6.46QDD357 pKa = 3.56AMNFWLQVGTGQADD371 pKa = 3.66PAEE374 pKa = 5.06DD375 pKa = 4.15GCDD378 pKa = 3.11HH379 pKa = 6.32VVPDD383 pKa = 4.52SGWADD388 pKa = 3.06SVMVVHH394 pKa = 6.63VDD396 pKa = 3.44SFRR399 pKa = 11.84DD400 pKa = 3.55CAMGGSNVFSGEE412 pKa = 3.84NTSFSTIRR420 pKa = 11.84HH421 pKa = 5.42EE422 pKa = 4.54SGHH425 pKa = 6.14RR426 pKa = 11.84PFGLADD432 pKa = 4.44EE433 pKa = 4.77YY434 pKa = 11.47CCDD437 pKa = 3.18GGYY440 pKa = 9.59YY441 pKa = 9.12QQDD444 pKa = 4.11VYY446 pKa = 11.29PNVYY450 pKa = 10.36DD451 pKa = 4.27EE452 pKa = 5.66PEE454 pKa = 4.56DD455 pKa = 4.2CADD458 pKa = 4.4DD459 pKa = 5.13APDD462 pKa = 4.11LGRR465 pKa = 11.84TASDD469 pKa = 3.04CRR471 pKa = 11.84EE472 pKa = 3.85FEE474 pKa = 4.23EE475 pKa = 6.0EE476 pKa = 4.09IEE478 pKa = 4.11NWDD481 pKa = 3.71DD482 pKa = 3.9FDD484 pKa = 4.42WSVSDD489 pKa = 4.18PATNDD494 pKa = 3.24LMVDD498 pKa = 3.25NTTAQAADD506 pKa = 3.38TRR508 pKa = 11.84RR509 pKa = 11.84IEE511 pKa = 3.94WYY513 pKa = 9.66FAEE516 pKa = 4.94CRR518 pKa = 11.84GAKK521 pKa = 9.57CC522 pKa = 4.55

Molecular weight: 57.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N6R361|A0A1N6R361_9ACTN Dihydropteroate synthase OS=Micromonospora avicenniae OX=1198245 GN=SAMN05444858_101576 PE=3 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.49KK7 pKa = 9.45RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.48RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.7LLRR22 pKa = 11.84KK23 pKa = 7.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LGKK33 pKa = 10.04

Molecular weight: 4.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6031

0

6031

1997338

24

10050

331.2

35.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.771 ± 0.048

0.681 ± 0.008

6.064 ± 0.028

5.21 ± 0.028

2.659 ± 0.016

9.403 ± 0.031

2.061 ± 0.014

3.266 ± 0.022

1.713 ± 0.026

10.414 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.625 ± 0.012

1.881 ± 0.022

6.391 ± 0.034

2.808 ± 0.019

8.382 ± 0.041

4.83 ± 0.026

6.117 ± 0.028

8.992 ± 0.033

1.589 ± 0.013

2.143 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski