Amino acid dipepetide frequency for Micromonospora avicenniae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.363AlaAla: 22.363 ± 0.168
0.837AlaCys: 0.837 ± 0.023
8.71AlaAsp: 8.71 ± 0.061
8.507AlaGlu: 8.507 ± 0.089
3.267AlaPhe: 3.267 ± 0.049
14.479AlaGly: 14.479 ± 0.109
2.402AlaHis: 2.402 ± 0.036
3.998AlaIle: 3.998 ± 0.049
2.321AlaLys: 2.321 ± 0.043
14.123AlaLeu: 14.123 ± 0.126
2.435AlaMet: 2.435 ± 0.036
2.279AlaAsn: 2.279 ± 0.04
7.205AlaPro: 7.205 ± 0.081
3.657AlaGln: 3.657 ± 0.046
10.242AlaArg: 10.242 ± 0.087
5.619AlaSer: 5.619 ± 0.056
7.934AlaThr: 7.934 ± 0.077
12.597AlaVal: 12.597 ± 0.101
1.865AlaTrp: 1.865 ± 0.034
2.869AlaTyr: 2.869 ± 0.033
0.0AlaXaa: 0.0 ± 0.0
Cys
0.871CysAla: 0.871 ± 0.024
0.108CysCys: 0.108 ± 0.009
0.455CysAsp: 0.455 ± 0.015
0.294CysGlu: 0.294 ± 0.014
0.185CysPhe: 0.185 ± 0.01
0.845CysGly: 0.845 ± 0.022
0.184CysHis: 0.184 ± 0.009
0.132CysIle: 0.132 ± 0.01
0.072CysLys: 0.072 ± 0.006
0.634CysLeu: 0.634 ± 0.019
0.095CysMet: 0.095 ± 0.007
0.132CysAsn: 0.132 ± 0.009
0.451CysPro: 0.451 ± 0.017
0.178CysGln: 0.178 ± 0.009
0.549CysArg: 0.549 ± 0.019
0.359CysSer: 0.359 ± 0.014
0.408CysThr: 0.408 ± 0.015
0.573CysVal: 0.573 ± 0.02
0.124CysTrp: 0.124 ± 0.008
0.161CysTyr: 0.161 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.565AspAla: 7.565 ± 0.072
0.359AspCys: 0.359 ± 0.014
3.741AspAsp: 3.741 ± 0.055
3.886AspGlu: 3.886 ± 0.05
1.519AspPhe: 1.519 ± 0.027
6.612AspGly: 6.612 ± 0.079
1.2AspHis: 1.2 ± 0.029
1.656AspIle: 1.656 ± 0.027
0.947AspLys: 0.947 ± 0.026
6.642AspLeu: 6.642 ± 0.068
0.69AspMet: 0.69 ± 0.019
0.989AspAsn: 0.989 ± 0.027
4.967AspPro: 4.967 ± 0.065
1.818AspGln: 1.818 ± 0.031
5.492AspArg: 5.492 ± 0.06
2.368AspSer: 2.368 ± 0.035
2.891AspThr: 2.891 ± 0.043
5.102AspVal: 5.102 ± 0.062
1.043AspTrp: 1.043 ± 0.024
1.151AspTyr: 1.151 ± 0.028
0.0AspXaa: 0.0 ± 0.0
Glu
6.496GluAla: 6.496 ± 0.072
0.334GluCys: 0.334 ± 0.014
2.148GluAsp: 2.148 ± 0.037
2.637GluGlu: 2.637 ± 0.047
1.487GluPhe: 1.487 ± 0.03
3.263GluGly: 3.263 ± 0.044
1.401GluHis: 1.401 ± 0.028
2.219GluIle: 2.219 ± 0.034
1.081GluLys: 1.081 ± 0.027
6.658GluLeu: 6.658 ± 0.064
0.83GluMet: 0.83 ± 0.021
0.909GluAsn: 0.909 ± 0.021
3.538GluPro: 3.538 ± 0.05
2.284GluGln: 2.284 ± 0.04
5.244GluArg: 5.244 ± 0.068
2.356GluSer: 2.356 ± 0.039
2.632GluThr: 2.632 ± 0.041
4.705GluVal: 4.705 ± 0.051
0.753GluTrp: 0.753 ± 0.022
1.12GluTyr: 1.12 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
3.626PheAla: 3.626 ± 0.045
0.227PheCys: 0.227 ± 0.009
1.962PheAsp: 1.962 ± 0.037
1.238PheGlu: 1.238 ± 0.026
0.815PhePhe: 0.815 ± 0.025
2.999PheGly: 2.999 ± 0.042
0.524PheHis: 0.524 ± 0.015
0.736PheIle: 0.736 ± 0.019
0.4PheLys: 0.4 ± 0.014
2.487PheLeu: 2.487 ± 0.044
0.338PheMet: 0.338 ± 0.014
0.613PheAsn: 0.613 ± 0.017
1.352PhePro: 1.352 ± 0.025
0.666PheGln: 0.666 ± 0.018
1.815PheArg: 1.815 ± 0.033
1.306PheSer: 1.306 ± 0.03
1.982PheThr: 1.982 ± 0.034
2.422PheVal: 2.422 ± 0.04
0.449PheTrp: 0.449 ± 0.015
0.631PheTyr: 0.631 ± 0.021
0.0PheXaa: 0.0 ± 0.0
Gly
10.884GlyAla: 10.884 ± 0.1
0.764GlyCys: 0.764 ± 0.022
5.364GlyAsp: 5.364 ± 0.068
4.876GlyGlu: 4.876 ± 0.05
2.752GlyPhe: 2.752 ± 0.04
8.928GlyGly: 8.928 ± 0.1
2.075GlyHis: 2.075 ± 0.031
3.177GlyIle: 3.177 ± 0.042
2.066GlyLys: 2.066 ± 0.041
9.422GlyLeu: 9.422 ± 0.086
1.908GlyMet: 1.908 ± 0.032
1.898GlyAsn: 1.898 ± 0.041
5.369GlyPro: 5.369 ± 0.067
2.88GlyGln: 2.88 ± 0.055
8.183GlyArg: 8.183 ± 0.077
5.043GlySer: 5.043 ± 0.061
5.879GlyThr: 5.879 ± 0.073
8.165GlyVal: 8.165 ± 0.073
1.927GlyTrp: 1.927 ± 0.032
2.47GlyTyr: 2.47 ± 0.04
0.0GlyXaa: 0.0 ± 0.0
His
2.356HisAla: 2.356 ± 0.037
0.166HisCys: 0.166 ± 0.009
1.283HisAsp: 1.283 ± 0.026
1.059HisGlu: 1.059 ± 0.023
0.566HisPhe: 0.566 ± 0.019
2.053HisGly: 2.053 ± 0.032
0.569HisHis: 0.569 ± 0.019
0.515HisIle: 0.515 ± 0.016
0.263HisLys: 0.263 ± 0.012
2.396HisLeu: 2.396 ± 0.04
0.251HisMet: 0.251 ± 0.013
0.371HisAsn: 0.371 ± 0.014
1.706HisPro: 1.706 ± 0.031
0.609HisGln: 0.609 ± 0.017
2.046HisArg: 2.046 ± 0.034
0.898HisSer: 0.898 ± 0.022
1.071HisThr: 1.071 ± 0.028
1.649HisVal: 1.649 ± 0.033
0.346HisTrp: 0.346 ± 0.012
0.439HisTyr: 0.439 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.832IleAla: 4.832 ± 0.06
0.283IleCys: 0.283 ± 0.011
2.454IleAsp: 2.454 ± 0.039
2.037IleGlu: 2.037 ± 0.034
0.896IlePhe: 0.896 ± 0.025
3.62IleGly: 3.62 ± 0.046
0.56IleHis: 0.56 ± 0.016
0.989IleIle: 0.989 ± 0.026
0.653IleLys: 0.653 ± 0.019
2.371IleLeu: 2.371 ± 0.035
0.442IleMet: 0.442 ± 0.013
0.745IleAsn: 0.745 ± 0.019
1.672IlePro: 1.672 ± 0.031
0.727IleGln: 0.727 ± 0.02
2.468IleArg: 2.468 ± 0.036
1.777IleSer: 1.777 ± 0.028
2.183IleThr: 2.183 ± 0.035
2.875IleVal: 2.875 ± 0.048
0.427IleTrp: 0.427 ± 0.015
0.65IleTyr: 0.65 ± 0.019
0.0IleXaa: 0.0 ± 0.0
Lys
2.17LysAla: 2.17 ± 0.043
0.1LysCys: 0.1 ± 0.007
0.881LysAsp: 0.881 ± 0.032
0.811LysGlu: 0.811 ± 0.024
0.422LysPhe: 0.422 ± 0.016
1.291LysGly: 1.291 ± 0.025
0.326LysHis: 0.326 ± 0.013
0.799LysIle: 0.799 ± 0.019
0.504LysLys: 0.504 ± 0.023
1.868LysLeu: 1.868 ± 0.037
0.302LysMet: 0.302 ± 0.011
0.391LysAsn: 0.391 ± 0.019
1.174LysPro: 1.174 ± 0.029
0.624LysGln: 0.624 ± 0.019
1.243LysArg: 1.243 ± 0.025
0.911LysSer: 0.911 ± 0.023
1.044LysThr: 1.044 ± 0.026
1.685LysVal: 1.685 ± 0.038
0.229LysTrp: 0.229 ± 0.011
0.356LysTyr: 0.356 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
16.262LeuAla: 16.262 ± 0.135
0.661LeuCys: 0.661 ± 0.019
6.853LeuAsp: 6.853 ± 0.074
3.858LeuGlu: 3.858 ± 0.053
2.625LeuPhe: 2.625 ± 0.044
9.313LeuGly: 9.313 ± 0.078
2.202LeuHis: 2.202 ± 0.035
3.504LeuIle: 3.504 ± 0.05
1.454LeuLys: 1.454 ± 0.032
11.735LeuLeu: 11.735 ± 0.125
1.398LeuMet: 1.398 ± 0.028
1.754LeuAsn: 1.754 ± 0.031
6.761LeuPro: 6.761 ± 0.071
1.935LeuGln: 1.935 ± 0.036
9.14LeuArg: 9.14 ± 0.079
4.909LeuSer: 4.909 ± 0.052
6.952LeuThr: 6.952 ± 0.066
9.799LeuVal: 9.799 ± 0.101
1.271LeuTrp: 1.271 ± 0.026
1.756LeuTyr: 1.756 ± 0.03
0.0LeuXaa: 0.0 ± 0.0
Met
2.075MetAla: 2.075 ± 0.036
0.102MetCys: 0.102 ± 0.009
0.731MetAsp: 0.731 ± 0.02
0.571MetGlu: 0.571 ± 0.017
0.444MetPhe: 0.444 ± 0.016
1.121MetGly: 1.121 ± 0.027
0.31MetHis: 0.31 ± 0.012
0.696MetIle: 0.696 ± 0.02
0.34MetLys: 0.34 ± 0.012
1.703MetLeu: 1.703 ± 0.03
0.275MetMet: 0.275 ± 0.011
0.374MetAsn: 0.374 ± 0.014
1.112MetPro: 1.112 ± 0.025
0.429MetGln: 0.429 ± 0.014
1.409MetArg: 1.409 ± 0.025
1.25MetSer: 1.25 ± 0.027
1.543MetThr: 1.543 ± 0.028
1.272MetVal: 1.272 ± 0.026
0.204MetTrp: 0.204 ± 0.011
0.288MetTyr: 0.288 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.234AsnAla: 2.234 ± 0.033
0.153AsnCys: 0.153 ± 0.009
1.02AsnAsp: 1.02 ± 0.026
0.839AsnGlu: 0.839 ± 0.022
0.535AsnPhe: 0.535 ± 0.016
1.988AsnGly: 1.988 ± 0.047
0.406AsnHis: 0.406 ± 0.013
0.621AsnIle: 0.621 ± 0.02
0.362AsnLys: 0.362 ± 0.015
1.906AsnLeu: 1.906 ± 0.036
0.311AsnMet: 0.311 ± 0.015
0.523AsnAsn: 0.523 ± 0.021
1.526AsnPro: 1.526 ± 0.03
0.577AsnGln: 0.577 ± 0.019
1.449AsnArg: 1.449 ± 0.026
0.994AsnSer: 0.994 ± 0.028
1.053AsnThr: 1.053 ± 0.031
1.513AsnVal: 1.513 ± 0.028
0.33AsnTrp: 0.33 ± 0.014
0.47AsnTyr: 0.47 ± 0.018
0.0AsnXaa: 0.0 ± 0.0
Pro
9.927ProAla: 9.927 ± 0.106
0.285ProCys: 0.285 ± 0.012
4.719ProAsp: 4.719 ± 0.055
4.108ProGlu: 4.108 ± 0.054
1.44ProPhe: 1.44 ± 0.028
6.665ProGly: 6.665 ± 0.081
1.212ProHis: 1.212 ± 0.025
1.715ProIle: 1.715 ± 0.029
1.032ProLys: 1.032 ± 0.028
5.284ProLeu: 5.284 ± 0.056
1.031ProMet: 1.031 ± 0.023
1.125ProAsn: 1.125 ± 0.026
4.247ProPro: 4.247 ± 0.086
1.724ProGln: 1.724 ± 0.032
4.111ProArg: 4.111 ± 0.045
3.12ProSer: 3.12 ± 0.048
3.968ProThr: 3.968 ± 0.056
5.905ProVal: 5.905 ± 0.055
0.929ProTrp: 0.929 ± 0.022
1.366ProTyr: 1.366 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
3.713GlnAla: 3.713 ± 0.045
0.135GlnCys: 0.135 ± 0.009
1.132GlnAsp: 1.132 ± 0.026
1.217GlnGlu: 1.217 ± 0.023
0.745GlnPhe: 0.745 ± 0.017
2.017GlnGly: 2.017 ± 0.031
0.627GlnHis: 0.627 ± 0.02
1.16GlnIle: 1.16 ± 0.029
0.487GlnLys: 0.487 ± 0.017
3.349GlnLeu: 3.349 ± 0.043
0.464GlnMet: 0.464 ± 0.015
0.52GlnAsn: 0.52 ± 0.017
2.018GlnPro: 2.018 ± 0.036
1.207GlnGln: 1.207 ± 0.028
2.739GlnArg: 2.739 ± 0.04
1.141GlnSer: 1.141 ± 0.025
1.41GlnThr: 1.41 ± 0.029
2.902GlnVal: 2.902 ± 0.038
0.495GlnTrp: 0.495 ± 0.016
0.599GlnTyr: 0.599 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
10.182ArgAla: 10.182 ± 0.09
0.576ArgCys: 0.576 ± 0.018
4.601ArgAsp: 4.601 ± 0.055
4.405ArgGlu: 4.405 ± 0.061
2.417ArgPhe: 2.417 ± 0.038
5.713ArgGly: 5.713 ± 0.062
2.119ArgHis: 2.119 ± 0.038
3.228ArgIle: 3.228 ± 0.043
1.332ArgLys: 1.332 ± 0.029
9.343ArgLeu: 9.343 ± 0.091
1.846ArgMet: 1.846 ± 0.034
1.53ArgAsn: 1.53 ± 0.032
5.652ArgPro: 5.652 ± 0.063
2.721ArgGln: 2.721 ± 0.04
8.783ArgArg: 8.783 ± 0.097
4.198ArgSer: 4.198 ± 0.042
4.903ArgThr: 4.903 ± 0.055
6.497ArgVal: 6.497 ± 0.06
1.667ArgTrp: 1.667 ± 0.029
2.106ArgTyr: 2.106 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
6.467SerAla: 6.467 ± 0.064
0.363SerCys: 0.363 ± 0.013
2.552SerAsp: 2.552 ± 0.039
2.162SerGlu: 2.162 ± 0.038
1.444SerPhe: 1.444 ± 0.028
5.603SerGly: 5.603 ± 0.058
0.889SerHis: 0.889 ± 0.023
1.559SerIle: 1.559 ± 0.03
0.805SerLys: 0.805 ± 0.024
4.289SerLeu: 4.289 ± 0.046
1.014SerMet: 1.014 ± 0.022
0.921SerAsn: 0.921 ± 0.026
3.152SerPro: 3.152 ± 0.047
1.226SerGln: 1.226 ± 0.024
3.788SerArg: 3.788 ± 0.047
2.626SerSer: 2.626 ± 0.047
3.161SerThr: 3.161 ± 0.049
4.119SerVal: 4.119 ± 0.052
0.947SerTrp: 0.947 ± 0.023
1.218SerTyr: 1.218 ± 0.027
0.0SerXaa: 0.0 ± 0.0
Thr
8.487ThrAla: 8.487 ± 0.086
0.38ThrCys: 0.38 ± 0.015
3.774ThrAsp: 3.774 ± 0.057
3.048ThrGlu: 3.048 ± 0.045
1.642ThrPhe: 1.642 ± 0.031
6.724ThrGly: 6.724 ± 0.073
1.005ThrHis: 1.005 ± 0.023
1.991ThrIle: 1.991 ± 0.034
0.987ThrLys: 0.987 ± 0.029
5.783ThrLeu: 5.783 ± 0.057
0.969ThrMet: 0.969 ± 0.024
1.146ThrAsn: 1.146 ± 0.03
4.152ThrPro: 4.152 ± 0.053
1.288ThrGln: 1.288 ± 0.028
4.199ThrArg: 4.199 ± 0.05
3.123ThrSer: 3.123 ± 0.043
3.81ThrThr: 3.81 ± 0.06
6.359ThrVal: 6.359 ± 0.061
0.971ThrTrp: 0.971 ± 0.024
1.334ThrTyr: 1.334 ± 0.03
0.0ThrXaa: 0.0 ± 0.0
Val
12.541ValAla: 12.541 ± 0.114
0.652ValCys: 0.652 ± 0.02
5.902ValAsp: 5.902 ± 0.052
4.841ValGlu: 4.841 ± 0.055
2.245ValPhe: 2.245 ± 0.041
7.61ValGly: 7.61 ± 0.079
1.78ValHis: 1.78 ± 0.035
2.954ValIle: 2.954 ± 0.041
1.44ValLys: 1.44 ± 0.032
9.817ValLeu: 9.817 ± 0.084
1.195ValMet: 1.195 ± 0.024
1.778ValAsn: 1.778 ± 0.033
5.66ValPro: 5.66 ± 0.063
2.094ValGln: 2.094 ± 0.035
7.291ValArg: 7.291 ± 0.062
4.325ValSer: 4.325 ± 0.053
6.155ValThr: 6.155 ± 0.058
8.904ValVal: 8.904 ± 0.09
1.169ValTrp: 1.169 ± 0.024
1.566ValTyr: 1.566 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.728TrpAla: 1.728 ± 0.034
0.155TrpCys: 0.155 ± 0.009
0.757TrpAsp: 0.757 ± 0.022
0.621TrpGlu: 0.621 ± 0.017
0.522TrpPhe: 0.522 ± 0.018
0.989TrpGly: 0.989 ± 0.026
0.393TrpHis: 0.393 ± 0.015
0.569TrpIle: 0.569 ± 0.017
0.279TrpLys: 0.279 ± 0.011
1.985TrpLeu: 1.985 ± 0.039
0.259TrpMet: 0.259 ± 0.01
0.412TrpAsn: 0.412 ± 0.016
0.918TrpPro: 0.918 ± 0.022
0.682TrpGln: 0.682 ± 0.018
1.605TrpArg: 1.605 ± 0.028
1.045TrpSer: 1.045 ± 0.023
1.048TrpThr: 1.048 ± 0.026
1.117TrpVal: 1.117 ± 0.023
0.397TrpTrp: 0.397 ± 0.015
0.412TrpTyr: 0.412 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.93TyrAla: 2.93 ± 0.039
0.169TyrCys: 0.169 ± 0.01
1.6TyrAsp: 1.6 ± 0.038
1.081TyrGlu: 1.081 ± 0.025
0.618TyrPhe: 0.618 ± 0.017
2.195TyrGly: 2.195 ± 0.041
0.429TyrHis: 0.429 ± 0.014
0.443TyrIle: 0.443 ± 0.016
0.305TyrLys: 0.305 ± 0.013
2.346TyrLeu: 2.346 ± 0.035
0.196TyrMet: 0.196 ± 0.009
0.401TyrAsn: 0.401 ± 0.019
1.285TyrPro: 1.285 ± 0.031
0.749TyrGln: 0.749 ± 0.02
2.024TyrArg: 2.024 ± 0.035
0.937TyrSer: 0.937 ± 0.023
1.146TyrThr: 1.146 ± 0.032
1.759TyrVal: 1.759 ± 0.031
0.351TyrTrp: 0.351 ± 0.016
0.465TyrTyr: 0.465 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6031 proteins (1997338 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski