Klebsiella phage KOX1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Webervirus; Klebsiella virus KOX1

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JSX9|A0A1W6JSX9_9CAUD Putative spanin OS=Klebsiella phage KOX1 OX=1970798 GN=KOX1_40 PE=4 SV=1
MM1 pKa = 7.89ADD3 pKa = 4.09NYY5 pKa = 11.56DD6 pKa = 3.29DD7 pKa = 5.81AYY9 pKa = 9.15WHH11 pKa = 7.04RR12 pKa = 11.84FLTAQDD18 pKa = 3.35AGLNRR23 pKa = 11.84EE24 pKa = 4.11YY25 pKa = 10.55CIKK28 pKa = 10.52VANQEE33 pKa = 4.08MTLSDD38 pKa = 3.92ALGSMDD44 pKa = 4.58MDD46 pKa = 4.27AEE48 pKa = 4.32SLPMRR53 pKa = 11.84DD54 pKa = 4.29DD55 pKa = 3.69LAEE58 pKa = 4.04QEE60 pKa = 4.67EE61 pKa = 4.57NSCGCTGEE69 pKa = 4.18EE70 pKa = 4.8GCRR73 pKa = 11.84EE74 pKa = 4.61CIPFWW79 pKa = 4.75

Molecular weight: 8.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JSW3|A0A1W6JSW3_9CAUD Uncharacterized protein OS=Klebsiella phage KOX1 OX=1970798 GN=KOX1_12 PE=4 SV=1
MM1 pKa = 7.33NHH3 pKa = 7.24PKK5 pKa = 9.42TDD7 pKa = 3.59SILAVLHH14 pKa = 4.85AHH16 pKa = 5.69GRR18 pKa = 11.84VVLRR22 pKa = 11.84MNRR25 pKa = 11.84EE26 pKa = 3.9SGFTQITITKK36 pKa = 8.84SNGRR40 pKa = 11.84YY41 pKa = 8.81VVGTVPGARR50 pKa = 11.84LIPSSLAGVTLTLEE64 pKa = 4.36SNSMFIEE71 pKa = 4.34SWRR74 pKa = 11.84SS75 pKa = 3.02

Molecular weight: 8.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

15514

39

1256

191.5

21.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.792 ± 0.439

1.373 ± 0.15

6.523 ± 0.213

6.401 ± 0.313

4.306 ± 0.151

7.78 ± 0.253

1.889 ± 0.202

6.156 ± 0.217

6.575 ± 0.381

6.375 ± 0.224

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.062 ± 0.156

4.448 ± 0.237

3.268 ± 0.212

3.906 ± 0.316

5.247 ± 0.205

6.149 ± 0.272

5.711 ± 0.296

7.065 ± 0.242

1.56 ± 0.099

3.416 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski