Amino acid dipepetide frequency for Klebsiella phage KOX1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.573AlaAla: 8.573 ± 1.128
0.967AlaCys: 0.967 ± 0.279
5.093AlaAsp: 5.093 ± 0.576
6.188AlaGlu: 6.188 ± 0.7
3.288AlaPhe: 3.288 ± 0.543
6.317AlaGly: 6.317 ± 0.674
1.354AlaHis: 1.354 ± 0.362
5.544AlaIle: 5.544 ± 0.621
6.769AlaLys: 6.769 ± 0.839
6.382AlaLeu: 6.382 ± 0.703
3.094AlaMet: 3.094 ± 0.435
3.159AlaAsn: 3.159 ± 0.468
2.901AlaPro: 2.901 ± 0.487
3.545AlaGln: 3.545 ± 0.715
4.706AlaArg: 4.706 ± 0.551
5.415AlaSer: 5.415 ± 0.643
4.835AlaThr: 4.835 ± 0.687
6.188AlaVal: 6.188 ± 0.773
1.289AlaTrp: 1.289 ± 0.266
2.321AlaTyr: 2.321 ± 0.364
0.0AlaXaa: 0.0 ± 0.0
Cys
0.645CysAla: 0.645 ± 0.213
0.258CysCys: 0.258 ± 0.132
1.612CysAsp: 1.612 ± 0.343
0.967CysGlu: 0.967 ± 0.285
0.387CysPhe: 0.387 ± 0.179
1.16CysGly: 1.16 ± 0.335
0.322CysHis: 0.322 ± 0.151
1.16CysIle: 1.16 ± 0.277
0.709CysLys: 0.709 ± 0.273
0.645CysLeu: 0.645 ± 0.2
0.322CysMet: 0.322 ± 0.142
0.258CysAsn: 0.258 ± 0.127
0.451CysPro: 0.451 ± 0.181
0.322CysGln: 0.322 ± 0.195
1.354CysArg: 1.354 ± 0.286
0.774CysSer: 0.774 ± 0.191
0.838CysThr: 0.838 ± 0.244
0.774CysVal: 0.774 ± 0.215
0.322CysTrp: 0.322 ± 0.143
0.451CysTyr: 0.451 ± 0.177
0.0CysXaa: 0.0 ± 0.0
Asp
6.382AspAla: 6.382 ± 0.584
0.645AspCys: 0.645 ± 0.177
4.383AspAsp: 4.383 ± 0.596
4.706AspGlu: 4.706 ± 0.542
3.03AspPhe: 3.03 ± 0.417
6.64AspGly: 6.64 ± 0.837
1.096AspHis: 1.096 ± 0.269
4.319AspIle: 4.319 ± 0.519
3.932AspLys: 3.932 ± 0.504
4.319AspLeu: 4.319 ± 0.554
1.225AspMet: 1.225 ± 0.322
2.321AspAsn: 2.321 ± 0.471
2.063AspPro: 2.063 ± 0.348
1.612AspGln: 1.612 ± 0.359
2.772AspArg: 2.772 ± 0.493
4.706AspSer: 4.706 ± 0.557
2.836AspThr: 2.836 ± 0.384
4.254AspVal: 4.254 ± 0.57
0.967AspTrp: 0.967 ± 0.263
3.03AspTyr: 3.03 ± 0.456
0.0AspXaa: 0.0 ± 0.0
Glu
4.77GluAla: 4.77 ± 0.521
0.838GluCys: 0.838 ± 0.25
3.481GluAsp: 3.481 ± 0.691
3.868GluGlu: 3.868 ± 0.62
4.512GluPhe: 4.512 ± 0.518
4.061GluGly: 4.061 ± 0.537
0.645GluHis: 0.645 ± 0.205
3.416GluIle: 3.416 ± 0.481
3.739GluLys: 3.739 ± 0.454
4.448GluLeu: 4.448 ± 0.714
2.643GluMet: 2.643 ± 0.432
2.514GluAsn: 2.514 ± 0.39
2.192GluPro: 2.192 ± 0.39
3.352GluGln: 3.352 ± 0.534
3.288GluArg: 3.288 ± 0.482
5.286GluSer: 5.286 ± 0.53
2.836GluThr: 2.836 ± 0.403
3.803GluVal: 3.803 ± 0.615
1.289GluTrp: 1.289 ± 0.314
3.03GluTyr: 3.03 ± 0.499
0.0GluXaa: 0.0 ± 0.0
Phe
3.03PheAla: 3.03 ± 0.426
0.709PheCys: 0.709 ± 0.202
3.416PheAsp: 3.416 ± 0.575
2.192PheGlu: 2.192 ± 0.411
1.676PhePhe: 1.676 ± 0.397
4.319PheGly: 4.319 ± 0.497
0.967PheHis: 0.967 ± 0.264
3.094PheIle: 3.094 ± 0.436
2.901PheLys: 2.901 ± 0.391
2.385PheLeu: 2.385 ± 0.379
0.902PheMet: 0.902 ± 0.263
2.578PheAsn: 2.578 ± 0.438
1.031PhePro: 1.031 ± 0.269
1.547PheGln: 1.547 ± 0.341
2.321PheArg: 2.321 ± 0.441
2.578PheSer: 2.578 ± 0.395
3.159PheThr: 3.159 ± 0.452
1.934PheVal: 1.934 ± 0.312
0.902PheTrp: 0.902 ± 0.279
1.418PheTyr: 1.418 ± 0.276
0.0PheXaa: 0.0 ± 0.0
Gly
5.286GlyAla: 5.286 ± 0.769
1.225GlyCys: 1.225 ± 0.317
4.835GlyAsp: 4.835 ± 0.479
5.479GlyGlu: 5.479 ± 0.565
2.836GlyPhe: 2.836 ± 0.374
8.058GlyGly: 8.058 ± 1.008
1.031GlyHis: 1.031 ± 0.318
4.706GlyIle: 4.706 ± 0.459
6.317GlyLys: 6.317 ± 0.574
4.577GlyLeu: 4.577 ± 0.437
2.514GlyMet: 2.514 ± 0.405
4.383GlyAsn: 4.383 ± 0.481
1.483GlyPro: 1.483 ± 0.255
2.063GlyGln: 2.063 ± 0.416
4.19GlyArg: 4.19 ± 0.433
4.77GlySer: 4.77 ± 0.671
3.997GlyThr: 3.997 ± 0.826
6.382GlyVal: 6.382 ± 0.616
1.225GlyTrp: 1.225 ± 0.294
2.45GlyTyr: 2.45 ± 0.394
0.0GlyXaa: 0.0 ± 0.0
His
1.418HisAla: 1.418 ± 0.4
0.258HisCys: 0.258 ± 0.133
1.289HisAsp: 1.289 ± 0.365
1.354HisGlu: 1.354 ± 0.334
0.709HisPhe: 0.709 ± 0.199
1.998HisGly: 1.998 ± 0.485
0.902HisHis: 0.902 ± 0.317
1.547HisIle: 1.547 ± 0.364
1.16HisLys: 1.16 ± 0.312
1.16HisLeu: 1.16 ± 0.386
0.064HisMet: 0.064 ± 0.072
0.774HisAsn: 0.774 ± 0.247
0.451HisPro: 0.451 ± 0.223
0.322HisGln: 0.322 ± 0.153
1.031HisArg: 1.031 ± 0.268
1.16HisSer: 1.16 ± 0.283
0.902HisThr: 0.902 ± 0.21
1.16HisVal: 1.16 ± 0.305
0.387HisTrp: 0.387 ± 0.172
0.838HisTyr: 0.838 ± 0.242
0.0HisXaa: 0.0 ± 0.0
Ile
6.317IleAla: 6.317 ± 0.725
1.031IleCys: 1.031 ± 0.263
4.448IleAsp: 4.448 ± 0.57
4.448IleGlu: 4.448 ± 0.562
2.192IlePhe: 2.192 ± 0.378
4.319IleGly: 4.319 ± 0.554
1.16IleHis: 1.16 ± 0.249
3.094IleIle: 3.094 ± 0.402
4.448IleLys: 4.448 ± 0.461
3.288IleLeu: 3.288 ± 0.461
1.934IleMet: 1.934 ± 0.385
3.288IleAsn: 3.288 ± 0.503
2.256IlePro: 2.256 ± 0.408
2.514IleGln: 2.514 ± 0.384
2.772IleArg: 2.772 ± 0.458
3.03IleSer: 3.03 ± 0.459
4.061IleThr: 4.061 ± 0.477
4.319IleVal: 4.319 ± 0.642
0.902IleTrp: 0.902 ± 0.275
1.74IleTyr: 1.74 ± 0.331
0.0IleXaa: 0.0 ± 0.0
Lys
6.059LysAla: 6.059 ± 0.565
0.902LysCys: 0.902 ± 0.303
4.061LysAsp: 4.061 ± 0.484
4.319LysGlu: 4.319 ± 0.415
2.321LysPhe: 2.321 ± 0.389
3.997LysGly: 3.997 ± 0.512
1.354LysHis: 1.354 ± 0.31
4.19LysIle: 4.19 ± 0.51
3.932LysLys: 3.932 ± 0.537
4.319LysLeu: 4.319 ± 0.512
3.416LysMet: 3.416 ± 0.598
2.385LysAsn: 2.385 ± 0.417
2.643LysPro: 2.643 ± 0.57
2.578LysGln: 2.578 ± 0.507
4.19LysArg: 4.19 ± 0.619
3.803LysSer: 3.803 ± 0.61
4.126LysThr: 4.126 ± 0.603
4.254LysVal: 4.254 ± 0.598
1.031LysTrp: 1.031 ± 0.295
1.869LysTyr: 1.869 ± 0.392
0.0LysXaa: 0.0 ± 0.0
Leu
6.897LeuAla: 6.897 ± 0.51
0.838LeuCys: 0.838 ± 0.254
3.739LeuAsp: 3.739 ± 0.55
3.545LeuGlu: 3.545 ± 0.554
2.385LeuPhe: 2.385 ± 0.341
4.254LeuGly: 4.254 ± 0.617
1.612LeuHis: 1.612 ± 0.428
3.61LeuIle: 3.61 ± 0.515
4.577LeuLys: 4.577 ± 0.696
3.481LeuLeu: 3.481 ± 0.446
1.483LeuMet: 1.483 ± 0.404
2.836LeuAsn: 2.836 ± 0.423
2.965LeuPro: 2.965 ± 0.475
2.385LeuGln: 2.385 ± 0.389
3.352LeuArg: 3.352 ± 0.445
4.577LeuSer: 4.577 ± 0.527
4.383LeuThr: 4.383 ± 0.565
4.19LeuVal: 4.19 ± 0.452
0.451LeuTrp: 0.451 ± 0.136
2.192LeuTyr: 2.192 ± 0.358
0.0LeuXaa: 0.0 ± 0.0
Met
3.545MetAla: 3.545 ± 0.527
0.193MetCys: 0.193 ± 0.105
1.289MetAsp: 1.289 ± 0.218
1.354MetGlu: 1.354 ± 0.282
1.676MetPhe: 1.676 ± 0.315
1.74MetGly: 1.74 ± 0.455
0.838MetHis: 0.838 ± 0.261
2.127MetIle: 2.127 ± 0.354
2.256MetLys: 2.256 ± 0.36
2.192MetLeu: 2.192 ± 0.399
1.031MetMet: 1.031 ± 0.298
1.74MetAsn: 1.74 ± 0.317
0.838MetPro: 0.838 ± 0.201
1.676MetGln: 1.676 ± 0.4
2.063MetArg: 2.063 ± 0.386
1.289MetSer: 1.289 ± 0.291
1.934MetThr: 1.934 ± 0.328
1.612MetVal: 1.612 ± 0.302
0.516MetTrp: 0.516 ± 0.193
0.709MetTyr: 0.709 ± 0.231
0.0MetXaa: 0.0 ± 0.0
Asn
3.674AsnAla: 3.674 ± 0.571
0.322AsnCys: 0.322 ± 0.151
2.45AsnAsp: 2.45 ± 0.317
2.836AsnGlu: 2.836 ± 0.401
1.547AsnPhe: 1.547 ± 0.317
5.221AsnGly: 5.221 ± 0.588
0.709AsnHis: 0.709 ± 0.247
2.321AsnIle: 2.321 ± 0.409
3.03AsnLys: 3.03 ± 0.491
2.321AsnLeu: 2.321 ± 0.458
1.354AsnMet: 1.354 ± 0.368
2.514AsnAsn: 2.514 ± 0.445
1.547AsnPro: 1.547 ± 0.295
1.934AsnGln: 1.934 ± 0.407
2.385AsnArg: 2.385 ± 0.328
2.514AsnSer: 2.514 ± 0.481
2.643AsnThr: 2.643 ± 0.542
3.223AsnVal: 3.223 ± 0.453
0.516AsnTrp: 0.516 ± 0.155
1.418AsnTyr: 1.418 ± 0.318
0.0AsnXaa: 0.0 ± 0.0
Pro
1.547ProAla: 1.547 ± 0.448
0.387ProCys: 0.387 ± 0.155
3.159ProAsp: 3.159 ± 0.503
3.416ProGlu: 3.416 ± 0.624
1.547ProPhe: 1.547 ± 0.254
2.127ProGly: 2.127 ± 0.281
0.387ProHis: 0.387 ± 0.146
1.998ProIle: 1.998 ± 0.417
2.385ProLys: 2.385 ± 0.454
1.676ProLeu: 1.676 ± 0.337
0.645ProMet: 0.645 ± 0.225
1.16ProAsn: 1.16 ± 0.269
0.645ProPro: 0.645 ± 0.186
1.547ProGln: 1.547 ± 0.343
1.869ProArg: 1.869 ± 0.386
1.934ProSer: 1.934 ± 0.361
1.74ProThr: 1.74 ± 0.363
2.772ProVal: 2.772 ± 0.369
0.516ProTrp: 0.516 ± 0.21
1.225ProTyr: 1.225 ± 0.413
0.0ProXaa: 0.0 ± 0.0
Gln
3.481GlnAla: 3.481 ± 0.583
0.387GlnCys: 0.387 ± 0.172
2.643GlnAsp: 2.643 ± 0.381
1.805GlnGlu: 1.805 ± 0.339
1.289GlnPhe: 1.289 ± 0.305
1.805GlnGly: 1.805 ± 0.358
0.58GlnHis: 0.58 ± 0.201
3.094GlnIle: 3.094 ± 0.417
2.192GlnLys: 2.192 ± 0.486
2.965GlnLeu: 2.965 ± 0.476
1.612GlnMet: 1.612 ± 0.379
1.74GlnAsn: 1.74 ± 0.4
1.16GlnPro: 1.16 ± 0.38
2.321GlnGln: 2.321 ± 0.766
1.676GlnArg: 1.676 ± 0.287
2.578GlnSer: 2.578 ± 0.667
2.256GlnThr: 2.256 ± 0.355
3.159GlnVal: 3.159 ± 0.7
0.516GlnTrp: 0.516 ± 0.212
1.805GlnTyr: 1.805 ± 0.286
0.0GlnXaa: 0.0 ± 0.0
Arg
4.19ArgAla: 4.19 ± 0.529
0.967ArgCys: 0.967 ± 0.369
4.061ArgAsp: 4.061 ± 0.593
3.932ArgGlu: 3.932 ± 0.627
2.836ArgPhe: 2.836 ± 0.405
3.932ArgGly: 3.932 ± 0.506
0.645ArgHis: 0.645 ± 0.244
2.707ArgIle: 2.707 ± 0.513
3.868ArgLys: 3.868 ± 0.473
3.288ArgLeu: 3.288 ± 0.587
1.74ArgMet: 1.74 ± 0.43
1.934ArgAsn: 1.934 ± 0.272
1.612ArgPro: 1.612 ± 0.359
1.934ArgGln: 1.934 ± 0.45
2.965ArgArg: 2.965 ± 0.471
2.643ArgSer: 2.643 ± 0.444
2.321ArgThr: 2.321 ± 0.433
4.448ArgVal: 4.448 ± 0.598
0.645ArgTrp: 0.645 ± 0.196
1.805ArgTyr: 1.805 ± 0.338
0.0ArgXaa: 0.0 ± 0.0
Ser
5.995SerAla: 5.995 ± 0.995
1.096SerCys: 1.096 ± 0.264
4.254SerAsp: 4.254 ± 0.528
4.19SerGlu: 4.19 ± 0.413
2.901SerPhe: 2.901 ± 0.364
5.35SerGly: 5.35 ± 0.513
1.483SerHis: 1.483 ± 0.389
3.223SerIle: 3.223 ± 0.525
3.61SerLys: 3.61 ± 0.525
4.254SerLeu: 4.254 ± 0.566
1.74SerMet: 1.74 ± 0.344
2.643SerAsn: 2.643 ± 0.462
1.805SerPro: 1.805 ± 0.39
2.45SerGln: 2.45 ± 0.447
2.514SerArg: 2.514 ± 0.399
3.094SerSer: 3.094 ± 0.518
3.416SerThr: 3.416 ± 0.598
4.254SerVal: 4.254 ± 0.451
1.225SerTrp: 1.225 ± 0.272
1.998SerTyr: 1.998 ± 0.405
0.0SerXaa: 0.0 ± 0.0
Thr
5.093ThrAla: 5.093 ± 0.656
1.031ThrCys: 1.031 ± 0.289
3.932ThrAsp: 3.932 ± 0.499
2.45ThrGlu: 2.45 ± 0.344
3.03ThrPhe: 3.03 ± 0.46
4.899ThrGly: 4.899 ± 0.715
1.418ThrHis: 1.418 ± 0.341
3.61ThrIle: 3.61 ± 0.47
3.159ThrLys: 3.159 ± 0.546
3.803ThrLeu: 3.803 ± 0.563
1.676ThrMet: 1.676 ± 0.338
2.385ThrAsn: 2.385 ± 0.339
2.256ThrPro: 2.256 ± 0.384
2.256ThrGln: 2.256 ± 0.419
2.45ThrArg: 2.45 ± 0.412
3.416ThrSer: 3.416 ± 0.595
2.643ThrThr: 2.643 ± 0.395
4.899ThrVal: 4.899 ± 0.602
0.387ThrTrp: 0.387 ± 0.143
2.256ThrTyr: 2.256 ± 0.355
0.0ThrXaa: 0.0 ± 0.0
Val
6.446ValAla: 6.446 ± 0.754
0.902ValCys: 0.902 ± 0.242
4.061ValAsp: 4.061 ± 0.553
4.448ValGlu: 4.448 ± 0.545
2.514ValPhe: 2.514 ± 0.37
4.512ValGly: 4.512 ± 0.669
0.967ValHis: 0.967 ± 0.234
5.286ValIle: 5.286 ± 0.553
3.803ValLys: 3.803 ± 0.625
4.577ValLeu: 4.577 ± 0.439
1.805ValMet: 1.805 ± 0.326
3.932ValAsn: 3.932 ± 0.46
2.45ValPro: 2.45 ± 0.426
2.063ValGln: 2.063 ± 0.571
3.223ValArg: 3.223 ± 0.416
5.221ValSer: 5.221 ± 0.666
5.093ValThr: 5.093 ± 0.6
5.802ValVal: 5.802 ± 0.665
1.354ValTrp: 1.354 ± 0.292
2.192ValTyr: 2.192 ± 0.374
0.0ValXaa: 0.0 ± 0.0
Trp
1.74TrpAla: 1.74 ± 0.342
0.387TrpCys: 0.387 ± 0.152
0.774TrpAsp: 0.774 ± 0.231
0.58TrpGlu: 0.58 ± 0.253
0.967TrpPhe: 0.967 ± 0.234
0.709TrpGly: 0.709 ± 0.206
0.709TrpHis: 0.709 ± 0.213
0.516TrpIle: 0.516 ± 0.176
0.967TrpLys: 0.967 ± 0.258
1.354TrpLeu: 1.354 ± 0.283
0.322TrpMet: 0.322 ± 0.142
0.451TrpAsn: 0.451 ± 0.212
0.387TrpPro: 0.387 ± 0.148
0.902TrpGln: 0.902 ± 0.222
1.418TrpArg: 1.418 ± 0.287
0.838TrpSer: 0.838 ± 0.278
0.709TrpThr: 0.709 ± 0.215
1.16TrpVal: 1.16 ± 0.24
0.322TrpTrp: 0.322 ± 0.151
0.387TrpTyr: 0.387 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.836TyrAla: 2.836 ± 0.542
0.387TyrCys: 0.387 ± 0.184
2.256TyrAsp: 2.256 ± 0.322
1.934TyrGlu: 1.934 ± 0.355
1.418TyrPhe: 1.418 ± 0.31
2.385TyrGly: 2.385 ± 0.384
0.709TyrHis: 0.709 ± 0.221
1.998TyrIle: 1.998 ± 0.261
1.998TyrLys: 1.998 ± 0.428
2.321TyrLeu: 2.321 ± 0.408
1.031TyrMet: 1.031 ± 0.257
1.483TyrAsn: 1.483 ± 0.444
1.547TyrPro: 1.547 ± 0.297
1.74TyrGln: 1.74 ± 0.297
1.934TyrArg: 1.934 ± 0.327
1.869TyrSer: 1.869 ± 0.302
2.385TyrThr: 2.385 ± 0.343
2.063TyrVal: 2.063 ± 0.305
0.838TyrTrp: 0.838 ± 0.219
1.031TyrTyr: 1.031 ± 0.273
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 81 proteins (15514 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski