Alkalibaculum sp. M08DMB

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Alkalibaculum

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6A7K946|A0A6A7K946_9FIRM Penicillin-binding transpeptidase domain-containing protein OS=Alkalibaculum sp. M08DMB OX=2655001 GN=GC105_09360 PE=3 SV=1
MM1 pKa = 7.72GLGFLDD7 pKa = 6.06NIFCDD12 pKa = 4.69DD13 pKa = 3.79NTLLIIILILVVVLLFSGGNFCCPGFSFGKK43 pKa = 10.52FNLGDD48 pKa = 3.36NWLLIVVVVLVLFGDD63 pKa = 4.09GLKK66 pKa = 10.88LFF68 pKa = 4.74

Molecular weight: 7.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6A7K7H6|A0A6A7K7H6_9FIRM MarR family transcriptional regulator OS=Alkalibaculum sp. M08DMB OX=2655001 GN=GC105_06625 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.56MTYY5 pKa = 8.6QPKK8 pKa = 8.64KK9 pKa = 7.79RR10 pKa = 11.84KK11 pKa = 9.02RR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.41GFRR19 pKa = 11.84KK20 pKa = 10.09RR21 pKa = 11.84MATKK25 pKa = 9.99SGRR28 pKa = 11.84NILSEE33 pKa = 4.0RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.91GRR39 pKa = 11.84KK40 pKa = 8.92RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3201

0

3201

969114

31

2871

302.8

34.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.726 ± 0.05

1.147 ± 0.019

5.545 ± 0.037

7.005 ± 0.045

4.269 ± 0.033

6.554 ± 0.046

1.624 ± 0.018

10.175 ± 0.048

7.783 ± 0.043

9.227 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.798 ± 0.022

5.738 ± 0.037

3.021 ± 0.023

2.913 ± 0.021

3.373 ± 0.025

6.479 ± 0.028

5.317 ± 0.041

6.545 ± 0.034

0.723 ± 0.014

4.037 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski