Amino acid dipepetide frequency for Alkalibaculum sp. M08DMB

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.926AlaAla: 3.926 ± 0.085
0.729AlaCys: 0.729 ± 0.03
2.708AlaAsp: 2.708 ± 0.058
2.979AlaGlu: 2.979 ± 0.064
2.506AlaPhe: 2.506 ± 0.051
4.118AlaGly: 4.118 ± 0.084
0.884AlaHis: 0.884 ± 0.031
6.082AlaIle: 6.082 ± 0.082
4.127AlaLys: 4.127 ± 0.071
5.92AlaLeu: 5.92 ± 0.084
1.846AlaMet: 1.846 ± 0.042
2.723AlaAsn: 2.723 ± 0.048
1.514AlaPro: 1.514 ± 0.041
1.826AlaGln: 1.826 ± 0.045
1.994AlaArg: 1.994 ± 0.054
3.582AlaSer: 3.582 ± 0.061
3.22AlaThr: 3.22 ± 0.065
4.066AlaVal: 4.066 ± 0.07
0.426AlaTrp: 0.426 ± 0.023
2.08AlaTyr: 2.08 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.656CysAla: 0.656 ± 0.026
0.16CysCys: 0.16 ± 0.013
0.681CysAsp: 0.681 ± 0.03
0.717CysGlu: 0.717 ± 0.028
0.494CysPhe: 0.494 ± 0.026
1.088CysGly: 1.088 ± 0.039
0.223CysHis: 0.223 ± 0.017
1.084CysIle: 1.084 ± 0.034
0.895CysLys: 0.895 ± 0.031
0.85CysLeu: 0.85 ± 0.031
0.286CysMet: 0.286 ± 0.019
0.635CysAsn: 0.635 ± 0.028
0.475CysPro: 0.475 ± 0.027
0.312CysGln: 0.312 ± 0.021
0.369CysArg: 0.369 ± 0.021
0.754CysSer: 0.754 ± 0.028
0.616CysThr: 0.616 ± 0.025
0.692CysVal: 0.692 ± 0.028
0.065CysTrp: 0.065 ± 0.009
0.416CysTyr: 0.416 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
2.806AspAla: 2.806 ± 0.061
0.625AspCys: 0.625 ± 0.029
2.758AspAsp: 2.758 ± 0.057
4.415AspGlu: 4.415 ± 0.066
2.541AspPhe: 2.541 ± 0.057
3.262AspGly: 3.262 ± 0.07
0.771AspHis: 0.771 ± 0.028
6.428AspIle: 6.428 ± 0.087
4.514AspLys: 4.514 ± 0.068
5.033AspLeu: 5.033 ± 0.079
1.594AspMet: 1.594 ± 0.042
3.109AspAsn: 3.109 ± 0.062
1.64AspPro: 1.64 ± 0.042
1.436AspGln: 1.436 ± 0.037
1.826AspArg: 1.826 ± 0.047
3.148AspSer: 3.148 ± 0.055
2.883AspThr: 2.883 ± 0.053
3.517AspVal: 3.517 ± 0.061
0.454AspTrp: 0.454 ± 0.023
2.693AspTyr: 2.693 ± 0.063
0.0AspXaa: 0.0 ± 0.0
Glu
3.741GluAla: 3.741 ± 0.059
0.646GluCys: 0.646 ± 0.028
4.007GluAsp: 4.007 ± 0.076
5.856GluGlu: 5.856 ± 0.092
2.723GluPhe: 2.723 ± 0.061
3.854GluGly: 3.854 ± 0.068
1.101GluHis: 1.101 ± 0.033
7.305GluIle: 7.305 ± 0.084
6.538GluLys: 6.538 ± 0.102
6.663GluLeu: 6.663 ± 0.097
2.122GluMet: 2.122 ± 0.052
4.523GluAsn: 4.523 ± 0.076
1.561GluPro: 1.561 ± 0.042
2.156GluGln: 2.156 ± 0.05
2.456GluArg: 2.456 ± 0.05
3.687GluSer: 3.687 ± 0.073
3.111GluThr: 3.111 ± 0.052
4.642GluVal: 4.642 ± 0.073
0.464GluTrp: 0.464 ± 0.022
2.899GluTyr: 2.899 ± 0.058
0.0GluXaa: 0.0 ± 0.0
Phe
2.43PheAla: 2.43 ± 0.052
0.536PheCys: 0.536 ± 0.025
2.527PheAsp: 2.527 ± 0.046
2.726PheGlu: 2.726 ± 0.046
1.992PhePhe: 1.992 ± 0.049
2.885PheGly: 2.885 ± 0.051
0.69PheHis: 0.69 ± 0.026
4.547PheIle: 4.547 ± 0.092
2.934PheLys: 2.934 ± 0.059
3.715PheLeu: 3.715 ± 0.065
1.179PheMet: 1.179 ± 0.034
2.467PheAsn: 2.467 ± 0.05
1.326PhePro: 1.326 ± 0.039
1.151PheGln: 1.151 ± 0.036
1.243PheArg: 1.243 ± 0.037
3.123PheSer: 3.123 ± 0.061
2.456PheThr: 2.456 ± 0.052
2.66PheVal: 2.66 ± 0.055
0.348PheTrp: 0.348 ± 0.021
1.758PheTyr: 1.758 ± 0.047
0.0PheXaa: 0.0 ± 0.0
Gly
4.224GlyAla: 4.224 ± 0.078
0.924GlyCys: 0.924 ± 0.035
3.237GlyAsp: 3.237 ± 0.065
3.979GlyGlu: 3.979 ± 0.063
3.107GlyPhe: 3.107 ± 0.059
4.688GlyGly: 4.688 ± 0.091
1.054GlyHis: 1.054 ± 0.034
6.942GlyIle: 6.942 ± 0.093
5.097GlyLys: 5.097 ± 0.071
5.892GlyLeu: 5.892 ± 0.086
1.995GlyMet: 1.995 ± 0.049
3.304GlyAsn: 3.304 ± 0.064
1.389GlyPro: 1.389 ± 0.044
1.788GlyGln: 1.788 ± 0.045
2.19GlyArg: 2.19 ± 0.051
3.924GlySer: 3.924 ± 0.067
3.67GlyThr: 3.67 ± 0.064
4.752GlyVal: 4.752 ± 0.068
0.491GlyTrp: 0.491 ± 0.026
2.89GlyTyr: 2.89 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
0.776HisAla: 0.776 ± 0.026
0.241HisCys: 0.241 ± 0.016
0.881HisAsp: 0.881 ± 0.028
1.042HisGlu: 1.042 ± 0.038
0.733HisPhe: 0.733 ± 0.03
1.13HisGly: 1.13 ± 0.039
0.391HisHis: 0.391 ± 0.021
1.624HisIle: 1.624 ± 0.042
1.062HisLys: 1.062 ± 0.036
1.478HisLeu: 1.478 ± 0.042
0.451HisMet: 0.451 ± 0.023
0.907HisAsn: 0.907 ± 0.035
0.755HisPro: 0.755 ± 0.026
0.468HisGln: 0.468 ± 0.023
0.627HisArg: 0.627 ± 0.026
1.039HisSer: 1.039 ± 0.032
0.856HisThr: 0.856 ± 0.027
0.974HisVal: 0.974 ± 0.03
0.122HisTrp: 0.122 ± 0.011
0.682HisTyr: 0.682 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
6.341IleAla: 6.341 ± 0.079
1.295IleCys: 1.295 ± 0.036
6.107IleAsp: 6.107 ± 0.084
7.124IleGlu: 7.124 ± 0.099
4.244IlePhe: 4.244 ± 0.088
6.473IleGly: 6.473 ± 0.084
1.567IleHis: 1.567 ± 0.043
10.087IleIle: 10.087 ± 0.129
7.543IleLys: 7.543 ± 0.101
9.257IleLeu: 9.257 ± 0.113
2.594IleMet: 2.594 ± 0.052
5.801IleAsn: 5.801 ± 0.075
3.648IlePro: 3.648 ± 0.063
2.847IleGln: 2.847 ± 0.055
3.264IleArg: 3.264 ± 0.061
7.374IleSer: 7.374 ± 0.083
5.556IleThr: 5.556 ± 0.081
6.515IleVal: 6.515 ± 0.094
0.579IleTrp: 0.579 ± 0.025
3.535IleTyr: 3.535 ± 0.066
0.0IleXaa: 0.0 ± 0.0
Lys
4.118LysAla: 4.118 ± 0.078
0.743LysCys: 0.743 ± 0.031
4.86LysAsp: 4.86 ± 0.075
7.097LysGlu: 7.097 ± 0.093
2.512LysPhe: 2.512 ± 0.047
4.507LysGly: 4.507 ± 0.07
1.18LysHis: 1.18 ± 0.033
7.292LysIle: 7.292 ± 0.088
6.651LysLys: 6.651 ± 0.089
6.641LysLeu: 6.641 ± 0.09
2.205LysMet: 2.205 ± 0.052
4.978LysAsn: 4.978 ± 0.072
2.012LysPro: 2.012 ± 0.048
2.289LysGln: 2.289 ± 0.046
2.756LysArg: 2.756 ± 0.054
4.816LysSer: 4.816 ± 0.072
3.817LysThr: 3.817 ± 0.052
5.265LysVal: 5.265 ± 0.072
0.543LysTrp: 0.543 ± 0.026
3.55LysTyr: 3.55 ± 0.065
0.0LysXaa: 0.0 ± 0.0
Leu
5.382LeuAla: 5.382 ± 0.078
1.017LeuCys: 1.017 ± 0.035
5.285LeuAsp: 5.285 ± 0.072
6.347LeuGlu: 6.347 ± 0.087
4.07LeuPhe: 4.07 ± 0.079
6.237LeuGly: 6.237 ± 0.072
1.496LeuHis: 1.496 ± 0.04
8.688LeuIle: 8.688 ± 0.112
7.014LeuLys: 7.014 ± 0.093
9.063LeuLeu: 9.063 ± 0.137
2.493LeuMet: 2.493 ± 0.053
5.271LeuAsn: 5.271 ± 0.073
3.005LeuPro: 3.005 ± 0.057
2.624LeuGln: 2.624 ± 0.057
3.072LeuArg: 3.072 ± 0.064
6.944LeuSer: 6.944 ± 0.092
4.854LeuThr: 4.854 ± 0.084
5.582LeuVal: 5.582 ± 0.083
0.613LeuTrp: 0.613 ± 0.024
3.215LeuTyr: 3.215 ± 0.068
0.0LeuXaa: 0.0 ± 0.0
Met
1.83MetAla: 1.83 ± 0.044
0.252MetCys: 0.252 ± 0.016
1.605MetAsp: 1.605 ± 0.043
1.923MetGlu: 1.923 ± 0.049
1.082MetPhe: 1.082 ± 0.031
1.975MetGly: 1.975 ± 0.053
0.394MetHis: 0.394 ± 0.019
2.628MetIle: 2.628 ± 0.053
2.447MetLys: 2.447 ± 0.05
2.336MetLeu: 2.336 ± 0.05
0.787MetMet: 0.787 ± 0.034
1.754MetAsn: 1.754 ± 0.041
1.003MetPro: 1.003 ± 0.031
0.748MetGln: 0.748 ± 0.025
0.965MetArg: 0.965 ± 0.031
1.92MetSer: 1.92 ± 0.043
1.463MetThr: 1.463 ± 0.039
1.802MetVal: 1.802 ± 0.048
0.166MetTrp: 0.166 ± 0.012
0.903MetTyr: 0.903 ± 0.032
0.0MetXaa: 0.0 ± 0.0
Asn
2.861AsnAla: 2.861 ± 0.057
0.62AsnCys: 0.62 ± 0.027
2.792AsnAsp: 2.792 ± 0.053
3.963AsnGlu: 3.963 ± 0.066
2.207AsnPhe: 2.207 ± 0.052
3.255AsnGly: 3.255 ± 0.058
0.967AsnHis: 0.967 ± 0.031
6.534AsnIle: 6.534 ± 0.096
4.799AsnLys: 4.799 ± 0.079
5.131AsnLeu: 5.131 ± 0.075
1.522AsnMet: 1.522 ± 0.044
3.735AsnAsn: 3.735 ± 0.08
2.082AsnPro: 2.082 ± 0.043
1.86AsnGln: 1.86 ± 0.053
2.051AsnArg: 2.051 ± 0.045
3.572AsnSer: 3.572 ± 0.064
3.087AsnThr: 3.087 ± 0.061
3.341AsnVal: 3.341 ± 0.055
0.465AsnTrp: 0.465 ± 0.022
2.527AsnTyr: 2.527 ± 0.054
0.0AsnXaa: 0.0 ± 0.0
Pro
1.531ProAla: 1.531 ± 0.052
0.359ProCys: 0.359 ± 0.02
1.775ProAsp: 1.775 ± 0.045
2.255ProGlu: 2.255 ± 0.059
1.419ProPhe: 1.419 ± 0.04
1.967ProGly: 1.967 ± 0.046
0.582ProHis: 0.582 ± 0.025
3.111ProIle: 3.111 ± 0.056
2.04ProLys: 2.04 ± 0.054
2.725ProLeu: 2.725 ± 0.057
0.835ProMet: 0.835 ± 0.03
1.576ProAsn: 1.576 ± 0.045
0.763ProPro: 0.763 ± 0.03
0.943ProGln: 0.943 ± 0.037
0.933ProArg: 0.933 ± 0.031
1.859ProSer: 1.859 ± 0.038
1.802ProThr: 1.802 ± 0.042
2.192ProVal: 2.192 ± 0.049
0.249ProTrp: 0.249 ± 0.016
1.294ProTyr: 1.294 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
1.656GlnAla: 1.656 ± 0.044
0.271GlnCys: 0.271 ± 0.017
1.54GlnAsp: 1.54 ± 0.038
2.29GlnGlu: 2.29 ± 0.053
1.097GlnPhe: 1.097 ± 0.033
1.832GlnGly: 1.832 ± 0.046
0.482GlnHis: 0.482 ± 0.023
2.829GlnIle: 2.829 ± 0.052
2.379GlnLys: 2.379 ± 0.055
2.744GlnLeu: 2.744 ± 0.058
0.831GlnMet: 0.831 ± 0.027
1.69GlnAsn: 1.69 ± 0.044
0.722GlnPro: 0.722 ± 0.03
0.963GlnGln: 0.963 ± 0.036
1.199GlnArg: 1.199 ± 0.037
1.753GlnSer: 1.753 ± 0.042
1.453GlnThr: 1.453 ± 0.042
1.872GlnVal: 1.872 ± 0.045
0.273GlnTrp: 0.273 ± 0.017
1.258GlnTyr: 1.258 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
1.808ArgAla: 1.808 ± 0.048
0.415ArgCys: 0.415 ± 0.02
1.901ArgAsp: 1.901 ± 0.05
2.586ArgGlu: 2.586 ± 0.054
1.417ArgPhe: 1.417 ± 0.041
2.144ArgGly: 2.144 ± 0.053
0.532ArgHis: 0.532 ± 0.023
3.242ArgIle: 3.242 ± 0.064
2.762ArgLys: 2.762 ± 0.055
3.101ArgLeu: 3.101 ± 0.067
0.99ArgMet: 0.99 ± 0.031
1.867ArgAsn: 1.867 ± 0.047
0.874ArgPro: 0.874 ± 0.033
1.074ArgGln: 1.074 ± 0.035
1.402ArgArg: 1.402 ± 0.045
1.841ArgSer: 1.841 ± 0.044
1.678ArgThr: 1.678 ± 0.041
2.403ArgVal: 2.403 ± 0.055
0.273ArgTrp: 0.273 ± 0.019
1.424ArgTyr: 1.424 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
3.421SerAla: 3.421 ± 0.065
0.694SerCys: 0.694 ± 0.027
3.316SerAsp: 3.316 ± 0.059
4.002SerGlu: 4.002 ± 0.068
3.049SerPhe: 3.049 ± 0.052
4.556SerGly: 4.556 ± 0.074
1.1SerHis: 1.1 ± 0.034
7.033SerIle: 7.033 ± 0.095
5.115SerLys: 5.115 ± 0.081
5.997SerLeu: 5.997 ± 0.086
1.868SerMet: 1.868 ± 0.044
3.531SerAsn: 3.531 ± 0.075
1.809SerPro: 1.809 ± 0.048
2.009SerGln: 2.009 ± 0.044
2.206SerArg: 2.206 ± 0.053
4.371SerSer: 4.371 ± 0.081
3.498SerThr: 3.498 ± 0.068
4.134SerVal: 4.134 ± 0.054
0.507SerTrp: 0.507 ± 0.026
2.573SerTyr: 2.573 ± 0.057
0.0SerXaa: 0.0 ± 0.0
Thr
3.201ThrAla: 3.201 ± 0.064
0.555ThrCys: 0.555 ± 0.027
2.766ThrAsp: 2.766 ± 0.055
3.045ThrGlu: 3.045 ± 0.064
2.225ThrPhe: 2.225 ± 0.055
3.974ThrGly: 3.974 ± 0.079
0.925ThrHis: 0.925 ± 0.037
5.408ThrIle: 5.408 ± 0.077
3.695ThrLys: 3.695 ± 0.059
5.078ThrLeu: 5.078 ± 0.074
1.406ThrMet: 1.406 ± 0.035
2.817ThrAsn: 2.817 ± 0.056
2.003ThrPro: 2.003 ± 0.045
1.601ThrGln: 1.601 ± 0.044
1.572ThrArg: 1.572 ± 0.043
3.468ThrSer: 3.468 ± 0.063
3.141ThrThr: 3.141 ± 0.08
3.709ThrVal: 3.709 ± 0.068
0.407ThrTrp: 0.407 ± 0.02
2.175ThrTyr: 2.175 ± 0.054
0.0ThrXaa: 0.0 ± 0.0
Val
3.996ValAla: 3.996 ± 0.062
0.808ValCys: 0.808 ± 0.029
3.9ValAsp: 3.9 ± 0.066
4.63ValGlu: 4.63 ± 0.072
3.01ValPhe: 3.01 ± 0.06
4.441ValGly: 4.441 ± 0.079
1.006ValHis: 1.006 ± 0.034
6.276ValIle: 6.276 ± 0.079
4.668ValLys: 4.668 ± 0.065
6.272ValLeu: 6.272 ± 0.088
1.726ValMet: 1.726 ± 0.048
3.588ValAsn: 3.588 ± 0.068
2.075ValPro: 2.075 ± 0.045
1.65ValGln: 1.65 ± 0.042
1.905ValArg: 1.905 ± 0.05
4.41ValSer: 4.41 ± 0.068
3.538ValThr: 3.538 ± 0.069
4.776ValVal: 4.776 ± 0.078
0.382ValTrp: 0.382 ± 0.017
2.388ValTyr: 2.388 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.393TrpAla: 0.393 ± 0.02
0.072TrpCys: 0.072 ± 0.009
0.447TrpAsp: 0.447 ± 0.023
0.468TrpGlu: 0.468 ± 0.025
0.349TrpPhe: 0.349 ± 0.018
0.526TrpGly: 0.526 ± 0.026
0.145TrpHis: 0.145 ± 0.013
0.702TrpIle: 0.702 ± 0.024
0.527TrpLys: 0.527 ± 0.023
0.652TrpLeu: 0.652 ± 0.027
0.238TrpMet: 0.238 ± 0.017
0.479TrpAsn: 0.479 ± 0.024
0.191TrpPro: 0.191 ± 0.016
0.24TrpGln: 0.24 ± 0.017
0.271TrpArg: 0.271 ± 0.016
0.435TrpSer: 0.435 ± 0.025
0.35TrpThr: 0.35 ± 0.024
0.41TrpVal: 0.41 ± 0.021
0.073TrpTrp: 0.073 ± 0.009
0.259TrpTyr: 0.259 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.16TyrAla: 2.16 ± 0.049
0.506TyrCys: 0.506 ± 0.026
2.362TyrAsp: 2.362 ± 0.044
2.608TyrGlu: 2.608 ± 0.054
1.917TyrPhe: 1.917 ± 0.046
2.627TyrGly: 2.627 ± 0.053
0.749TyrHis: 0.749 ± 0.025
3.907TyrIle: 3.907 ± 0.065
3.023TyrLys: 3.023 ± 0.057
3.725TyrLeu: 3.725 ± 0.069
1.014TyrMet: 1.014 ± 0.037
2.621TyrAsn: 2.621 ± 0.068
1.362TyrPro: 1.362 ± 0.042
1.147TyrGln: 1.147 ± 0.033
1.432TyrArg: 1.432 ± 0.042
2.766TyrSer: 2.766 ± 0.053
2.12TyrThr: 2.12 ± 0.048
2.143TyrVal: 2.143 ± 0.045
0.329TyrTrp: 0.329 ± 0.017
1.853TyrTyr: 1.853 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3201 proteins (969114 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski