Frigoribacterium sp. Leaf164

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Frigoribacterium; unclassified Frigoribacterium

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2793 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5LKL0|A0A0Q5LKL0_9MICO Ribonuclease P protein component OS=Frigoribacterium sp. Leaf164 OX=1736282 GN=rnpA PE=3 SV=1
MM1 pKa = 7.35GVLIIAASAAGIAAFGASTAPAPGARR27 pKa = 11.84LTPSEE32 pKa = 4.12QGEE35 pKa = 4.53TTVTVRR41 pKa = 11.84LWDD44 pKa = 3.45EE45 pKa = 4.14TVAAGYY51 pKa = 7.69EE52 pKa = 3.92KK53 pKa = 10.82SFAAFEE59 pKa = 4.37RR60 pKa = 11.84DD61 pKa = 3.49NPDD64 pKa = 2.47IDD66 pKa = 4.21VEE68 pKa = 4.67VEE70 pKa = 4.0VVPYY74 pKa = 10.95ADD76 pKa = 5.05YY77 pKa = 10.92FDD79 pKa = 4.6QLADD83 pKa = 4.41DD84 pKa = 4.77VDD86 pKa = 5.95DD87 pKa = 5.36GDD89 pKa = 4.97APDD92 pKa = 4.7VYY94 pKa = 10.36WLNSSHH100 pKa = 6.54YY101 pKa = 10.16DD102 pKa = 3.52GYY104 pKa = 11.76AEE106 pKa = 5.42AGDD109 pKa = 4.39LVDD112 pKa = 3.86VDD114 pKa = 4.4AVLGEE119 pKa = 4.17SARR122 pKa = 11.84RR123 pKa = 11.84AWSPAVVDD131 pKa = 3.89QFTHH135 pKa = 7.35DD136 pKa = 3.41GSLWGVPQLSDD147 pKa = 3.34GGIALYY153 pKa = 11.11YY154 pKa = 10.66NADD157 pKa = 3.73LLAAAGVDD165 pKa = 3.75PASLDD170 pKa = 3.75DD171 pKa = 4.28LTWAPGGGADD181 pKa = 5.03DD182 pKa = 3.96TLLPLLQRR190 pKa = 11.84LTLDD194 pKa = 3.58DD195 pKa = 3.7QGRR198 pKa = 11.84NAADD202 pKa = 3.45PAFDD206 pKa = 3.74GTVTQYY212 pKa = 11.36GYY214 pKa = 11.11NAGNDD219 pKa = 3.92LQAIWYY225 pKa = 8.09DD226 pKa = 3.8YY227 pKa = 10.95LGSAGGRR234 pKa = 11.84LEE236 pKa = 4.6DD237 pKa = 3.85PEE239 pKa = 4.5GASFEE244 pKa = 4.86LDD246 pKa = 3.3TPEE249 pKa = 3.95AQAAFQYY256 pKa = 10.94LVDD259 pKa = 4.05LVGVRR264 pKa = 11.84RR265 pKa = 11.84VAPPAAATNADD276 pKa = 3.92PDD278 pKa = 3.45HH279 pKa = 6.86SRR281 pKa = 11.84DD282 pKa = 3.67LFTAGHH288 pKa = 5.94MALFQSGLYY297 pKa = 9.85SLSTIEE303 pKa = 4.87SQAQFDD309 pKa = 3.89WGVAMMPAGPAGRR322 pKa = 11.84VSVSNGIVAAGDD334 pKa = 3.21AHH336 pKa = 6.15TAHH339 pKa = 7.35LDD341 pKa = 3.4DD342 pKa = 4.11TAKK345 pKa = 10.54VLAWLGSTDD354 pKa = 3.02GAEE357 pKa = 4.2YY358 pKa = 10.11IGRR361 pKa = 11.84DD362 pKa = 3.57GAAVPAVTGAQEE374 pKa = 3.64PYY376 pKa = 7.84YY377 pKa = 10.51AYY379 pKa = 8.86WASRR383 pKa = 11.84GVDD386 pKa = 3.03VSPFFDD392 pKa = 4.25VIASGDD398 pKa = 4.01TIPAPTSLYY407 pKa = 9.49GTAQPALDD415 pKa = 4.37PLLDD419 pKa = 3.61QVFAGSLPVPEE430 pKa = 4.92GLAAAQAAAEE440 pKa = 4.18AAVAAAEE447 pKa = 4.13QASDD451 pKa = 4.0PSATPAPP458 pKa = 4.07

Molecular weight: 47.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5LH32|A0A0Q5LH32_9MICO Peptide ABC transporter ATP-binding protein OS=Frigoribacterium sp. Leaf164 OX=1736282 GN=ASF82_14635 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.68GRR40 pKa = 11.84TEE42 pKa = 4.13LSAA45 pKa = 4.86

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2793

0

2793

897636

38

3668

321.4

34.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.947 ± 0.066

0.419 ± 0.01

6.559 ± 0.044

5.372 ± 0.049

2.832 ± 0.029

9.399 ± 0.047

1.903 ± 0.024

3.193 ± 0.042

1.653 ± 0.032

10.173 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.503 ± 0.017

1.508 ± 0.022

5.556 ± 0.037

2.681 ± 0.028

7.337 ± 0.054

5.909 ± 0.034

6.391 ± 0.061

10.466 ± 0.062

1.434 ± 0.019

1.764 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski