Pontibacter oryzae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Pontibacter

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3576 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A399S4Y5|A0A399S4Y5_9BACT Uncharacterized protein OS=Pontibacter oryzae OX=2304593 GN=D1627_12035 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 9.38KK3 pKa = 8.54TFVNCAAALVVSGLLFSCTTSDD25 pKa = 3.19STTDD29 pKa = 3.2SEE31 pKa = 4.95ADD33 pKa = 3.27INSEE37 pKa = 4.12TGTMDD42 pKa = 4.77DD43 pKa = 5.22DD44 pKa = 4.58YY45 pKa = 11.91GSTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTGTTTMM98 pKa = 4.56

Molecular weight: 9.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A399S033|A0A399S033_9BACT Sterol desaturase family protein OS=Pontibacter oryzae OX=2304593 GN=D1627_10355 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.54RR3 pKa = 11.84TFQPSQRR10 pKa = 11.84KK11 pKa = 8.72RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.56HH16 pKa = 3.97GFRR19 pKa = 11.84SRR21 pKa = 11.84MEE23 pKa = 3.95TANGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.54GRR39 pKa = 11.84KK40 pKa = 8.64RR41 pKa = 11.84LTVSSEE47 pKa = 3.88KK48 pKa = 9.99RR49 pKa = 11.84HH50 pKa = 5.45KK51 pKa = 10.57AAA53 pKa = 5.14

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3576

0

3576

1225943

30

3108

342.8

38.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.194 ± 0.048

0.719 ± 0.014

5.114 ± 0.033

6.431 ± 0.049

4.49 ± 0.028

6.87 ± 0.038

2.027 ± 0.023

6.102 ± 0.034

6.034 ± 0.043

10.156 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.022

4.804 ± 0.038

4.034 ± 0.026

4.4 ± 0.032

4.457 ± 0.029

6.088 ± 0.036

5.727 ± 0.056

6.812 ± 0.036

1.104 ± 0.015

4.039 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski