Alces alces faeces associated microvirus MP3 6497
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CHK4|A0A2Z5CHK4_9VIRU Minor capsid protein OS=Alces alces faeces associated microvirus MP3 6497 OX=2219139 PE=4 SV=1
MM1 pKa = 7.68 ANRR4 pKa = 11.84 YY5 pKa = 9.16 DD6 pKa = 4.22 RR7 pKa = 11.84 LDD9 pKa = 3.22 NTAPIEE15 pKa = 4.21 TALKK19 pKa = 9.98 ADD21 pKa = 3.76 SVSVGRR27 pKa = 11.84 SAFDD31 pKa = 3.08 WSCIHH36 pKa = 5.9 NTNGIIGSLIPIDD49 pKa = 4.31 CFDD52 pKa = 3.72 VVPNEE57 pKa = 4.66 DD58 pKa = 4.0 LDD60 pKa = 3.74 ISIASLIEE68 pKa = 3.79 FRR70 pKa = 11.84 NPTTRR75 pKa = 11.84 QLFNGMRR82 pKa = 11.84 VFVHH86 pKa = 7.01 AYY88 pKa = 8.93 YY89 pKa = 10.9 NRR91 pKa = 11.84 LTDD94 pKa = 3.22 LWEE97 pKa = 4.52 GARR100 pKa = 11.84 NWLDD104 pKa = 3.12 NGRR107 pKa = 11.84 TGKK110 pKa = 10.75 LSLTRR115 pKa = 11.84 PNLVYY120 pKa = 10.41 TGFYY124 pKa = 9.62 KK125 pKa = 10.77 APSNVTYY132 pKa = 10.47 RR133 pKa = 11.84 GNACTPMSLLDD144 pKa = 3.96 YY145 pKa = 10.76 LGLPAQRR152 pKa = 11.84 MQDD155 pKa = 3.38 TLKK158 pKa = 10.2 TSDD161 pKa = 3.47 GSVTASPLRR170 pKa = 11.84 SFQCAYY176 pKa = 9.4 WSSNNDD182 pKa = 2.99 FTASEE187 pKa = 4.31 LSYY190 pKa = 11.49 APDD193 pKa = 4.54 FFPADD198 pKa = 3.17 CCMAYY203 pKa = 9.78 QRR205 pKa = 11.84 NWRR208 pKa = 11.84 DD209 pKa = 3.53 FYY211 pKa = 11.56 CNKK214 pKa = 10.14 NLLQNNPYY222 pKa = 9.12 WFPEE226 pKa = 4.06 NEE228 pKa = 3.75 EE229 pKa = 4.26 HH230 pKa = 7.71 FILSYY235 pKa = 10.3 SCSDD239 pKa = 3.55 AVCVNYY245 pKa = 10.65 GNEE248 pKa = 4.07 NLSSVSANALNNIVGNIPTVIADD271 pKa = 3.46 YY272 pKa = 11.19 SVTPEE277 pKa = 4.09 PNNPITNANDD287 pKa = 3.37 YY288 pKa = 10.17 PGYY291 pKa = 10.23 IPTYY295 pKa = 10.12 APNLSGLKK303 pKa = 9.72 FRR305 pKa = 11.84 QFRR308 pKa = 11.84 GDD310 pKa = 3.22 RR311 pKa = 11.84 FTTALPFPDD320 pKa = 5.8 LIRR323 pKa = 11.84 GDD325 pKa = 3.89 IPILEE330 pKa = 4.24 YY331 pKa = 10.71 TSDD334 pKa = 3.45 NVASVTFTRR343 pKa = 11.84 SDD345 pKa = 3.64 DD346 pKa = 3.94 DD347 pKa = 4.27 SVEE350 pKa = 4.28 LDD352 pKa = 3.18 LHH354 pKa = 6.43 NNYY357 pKa = 10.34 SSTIGGTYY365 pKa = 10.12 VNTYY369 pKa = 10.37 LKK371 pKa = 10.77 DD372 pKa = 3.58 STVEE376 pKa = 3.78 NTDD379 pKa = 3.53 PFNVTATVDD388 pKa = 3.88 LSSLGITMSDD398 pKa = 2.92 IYY400 pKa = 10.96 TLEE403 pKa = 3.83 TLTAFRR409 pKa = 11.84 RR410 pKa = 11.84 RR411 pKa = 11.84 MGMTNGDD418 pKa = 4.04 YY419 pKa = 11.51 NEE421 pKa = 4.62 MIKK424 pKa = 10.49 AQYY427 pKa = 9.32 GVNPHH432 pKa = 4.24 VHH434 pKa = 6.41 DD435 pKa = 4.91 RR436 pKa = 11.84 KK437 pKa = 8.93 GTYY440 pKa = 9.11 IGGYY444 pKa = 7.88 YY445 pKa = 9.76 QDD447 pKa = 4.18 FAFSSVTQSSEE458 pKa = 4.03 TGSTPLGTKK467 pKa = 10.01 AGQGVSSGSGSIGHH481 pKa = 6.42 FHH483 pKa = 6.46 TPDD486 pKa = 3.57 FGWIQIYY493 pKa = 9.29 MSIVPDD499 pKa = 3.55 VYY501 pKa = 9.23 YY502 pKa = 9.76 TQGIPRR508 pKa = 11.84 MFSKK512 pKa = 10.66 KK513 pKa = 10.39 SNLEE517 pKa = 3.6 MYY519 pKa = 10.31 FPIFNNLPAQAILNKK534 pKa = 9.43 EE535 pKa = 4.36 LYY537 pKa = 10.54 VSGDD541 pKa = 3.27 SDD543 pKa = 3.49 VDD545 pKa = 3.43 NDD547 pKa = 3.39 VFAYY551 pKa = 8.88 EE552 pKa = 4.61 DD553 pKa = 3.91 RR554 pKa = 11.84 YY555 pKa = 11.09 AEE557 pKa = 4.07 YY558 pKa = 10.47 KK559 pKa = 10.12 SRR561 pKa = 11.84 HH562 pKa = 4.83 NRR564 pKa = 11.84 VSGLMALPVDD574 pKa = 3.79 KK575 pKa = 10.91 APYY578 pKa = 8.49 DD579 pKa = 3.68 TARR582 pKa = 11.84 VIARR586 pKa = 11.84 RR587 pKa = 11.84 FEE589 pKa = 4.53 SVPSLNSLFVTMVPEE604 pKa = 4.19 NVDD607 pKa = 3.24 MSPFSVTDD615 pKa = 3.72 EE616 pKa = 4.36 PPFDD620 pKa = 3.28 ISVGINCRR628 pKa = 11.84 RR629 pKa = 11.84 VFPAPYY635 pKa = 8.62 TAIEE639 pKa = 4.68 GSLSSPALVRR649 pKa = 11.84 GG650 pKa = 3.73
Molecular weight: 72.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.518
IPC2_protein 4.736
IPC_protein 4.711
Toseland 4.558
ProMoST 4.851
Dawson 4.698
Bjellqvist 4.838
Wikipedia 4.596
Rodwell 4.558
Grimsley 4.469
Solomon 4.698
Lehninger 4.647
Nozaki 4.8
DTASelect 5.041
Thurlkill 4.571
EMBOSS 4.622
Sillero 4.851
Patrickios 2.676
IPC_peptide 4.698
IPC2_peptide 4.838
IPC2.peptide.svr19 4.79
Protein with the highest isoelectric point:
>tr|A0A2Z5CJ66|A0A2Z5CJ66_9VIRU Replication initiation protein OS=Alces alces faeces associated microvirus MP3 6497 OX=2219139 PE=4 SV=1
MM1 pKa = 7.28 EE2 pKa = 6.12 RR3 pKa = 11.84 YY4 pKa = 9.99 FNMACARR11 pKa = 11.84 PLTILPKK18 pKa = 9.95 TRR20 pKa = 11.84 FNGDD24 pKa = 3.42 YY25 pKa = 9.92 IYY27 pKa = 10.59 TRR29 pKa = 11.84 EE30 pKa = 3.89 FTVPCGYY37 pKa = 10.41 CLNCRR42 pKa = 11.84 RR43 pKa = 11.84 DD44 pKa = 3.63 YY45 pKa = 11.13 QNYY48 pKa = 9.75 IIDD51 pKa = 3.82 RR52 pKa = 11.84 ANYY55 pKa = 7.63 EE56 pKa = 3.88 YY57 pKa = 10.69 CKK59 pKa = 10.28 RR60 pKa = 11.84 LSASFVTVTYY70 pKa = 11.14 DD71 pKa = 3.92 DD72 pKa = 3.07 IHH74 pKa = 8.39 LIEE77 pKa = 4.84 HH78 pKa = 6.34 CAVRR82 pKa = 11.84 DD83 pKa = 3.73 YY84 pKa = 11.45 DD85 pKa = 3.64 GSLIFDD91 pKa = 3.59 TDD93 pKa = 3.26 SRR95 pKa = 11.84 GEE97 pKa = 4.13 KK98 pKa = 9.58 IVRR101 pKa = 11.84 TTLNYY106 pKa = 10.21 SDD108 pKa = 3.73 FTRR111 pKa = 11.84 FVDD114 pKa = 4.39 NIRR117 pKa = 11.84 HH118 pKa = 5.54 YY119 pKa = 10.48 IVNHH123 pKa = 5.25 SEE125 pKa = 3.86 INNILCQPDD134 pKa = 4.37 FSYY137 pKa = 10.7 LYY139 pKa = 9.97 CGEE142 pKa = 4.5 YY143 pKa = 10.33 GDD145 pKa = 4.57 QFGRR149 pKa = 11.84 CHH151 pKa = 5.98 AHH153 pKa = 6.28 FLFFGLDD160 pKa = 3.18 FAYY163 pKa = 10.28 CKK165 pKa = 10.43 KK166 pKa = 10.56 IFMDD170 pKa = 2.87 KK171 pKa = 10.1 WKK173 pKa = 10.6 FGFVDD178 pKa = 3.79 VLPVLDD184 pKa = 3.66 GGIRR188 pKa = 11.84 YY189 pKa = 8.2 VCKK192 pKa = 10.91 YY193 pKa = 8.28 MDD195 pKa = 3.58 KK196 pKa = 10.85 FEE198 pKa = 5.52 KK199 pKa = 10.65 GFQAEE204 pKa = 4.27 LKK206 pKa = 10.54 YY207 pKa = 10.79 DD208 pKa = 4.17 FKK210 pKa = 11.63 GLARR214 pKa = 11.84 PKK216 pKa = 10.68 LRR218 pKa = 11.84 MSKK221 pKa = 10.65 GFGQGLLWNNAKK233 pKa = 10.35 KK234 pKa = 10.33 IIEE237 pKa = 4.11 NDD239 pKa = 2.95 YY240 pKa = 10.8 CYY242 pKa = 10.62 EE243 pKa = 4.03 SRR245 pKa = 11.84 HH246 pKa = 5.24 KK247 pKa = 9.98 QLRR250 pKa = 11.84 PISAYY255 pKa = 9.07 WKK257 pKa = 10.06 LLLTGGVPSRR267 pKa = 11.84 DD268 pKa = 3.41 VTKK271 pKa = 10.84 KK272 pKa = 10.74 DD273 pKa = 3.04 VWSKK277 pKa = 10.98 KK278 pKa = 10.3 DD279 pKa = 3.28 SYY281 pKa = 10.52 LQKK284 pKa = 10.64 KK285 pKa = 8.2 SASVVRR291 pKa = 11.84 SLHH294 pKa = 5.5 EE295 pKa = 4.28 LNITRR300 pKa = 11.84 DD301 pKa = 2.8 NGYY304 pKa = 9.25 PVDD307 pKa = 3.95 SDD309 pKa = 3.58 MFQSAFKK316 pKa = 10.36 LRR318 pKa = 11.84 CARR321 pKa = 11.84 IRR323 pKa = 11.84 EE324 pKa = 4.21 KK325 pKa = 10.89 NIEE328 pKa = 4.19 VQLHH332 pKa = 6.0 NNLTPIPLNHH342 pKa = 6.39 IPARR346 pKa = 11.84 PKK348 pKa = 10.44 FGSITYY354 pKa = 9.87 DD355 pKa = 3.22 YY356 pKa = 11.07 NKK358 pKa = 10.37 LKK360 pKa = 10.54 KK361 pKa = 10.54 CPTRR365 pKa = 11.84 TLRR368 pKa = 11.84 ALNYY372 pKa = 10.91 AFMEE376 pKa = 4.74 DD377 pKa = 4.61 LFSSLPKK384 pKa = 9.83 EE385 pKa = 4.42 YY386 pKa = 10.25 YY387 pKa = 9.67 FHH389 pKa = 8.07 SEE391 pKa = 3.87 VSS393 pKa = 3.24
Molecular weight: 46.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.24
IPC2_protein 8.258
IPC_protein 8.097
Toseland 8.053
ProMoST 8.609
Dawson 8.858
Bjellqvist 9.033
Wikipedia 8.887
Rodwell 8.916
Grimsley 8.097
Solomon 8.916
Lehninger 8.902
Nozaki 9.121
DTASelect 8.814
Thurlkill 8.873
EMBOSS 9.004
Sillero 9.136
Patrickios 3.973
IPC_peptide 8.902
IPC2_peptide 7.951
IPC2.peptide.svr19 8.025
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1363
320
650
454.3
51.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.236 ± 0.911
1.761 ± 0.785
7.117 ± 0.091
4.035 ± 0.194
5.062 ± 0.779
6.163 ± 0.538
1.761 ± 0.376
5.723 ± 0.338
4.916 ± 1.447
7.85 ± 0.349
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.861 ± 0.888
7.483 ± 0.891
4.549 ± 1.135
3.302 ± 1.287
5.356 ± 0.814
8.291 ± 0.869
5.943 ± 0.722
4.842 ± 1.247
1.174 ± 0.14
5.576 ± 1.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here