Escherichia phage Phieco32 (Escherichia coli phage phi32)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kuravirus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 128 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0FIK6|B0FIK6_BPE32 Uncharacterized protein OS=Escherichia phage Phieco32 OX=490103 GN=phi32_44 PE=4 SV=1
MM1 pKa = 7.79EE2 pKa = 5.82YY3 pKa = 9.52IDD5 pKa = 5.12KK6 pKa = 10.92YY7 pKa = 10.8SSEE10 pKa = 4.64DD11 pKa = 3.37STDD14 pKa = 3.56AEE16 pKa = 4.35VLFILKK22 pKa = 8.86TDD24 pKa = 4.05CLEE27 pKa = 4.28HH28 pKa = 6.09GQLINFIEE36 pKa = 4.11LAKK39 pKa = 10.64LKK41 pKa = 10.93EE42 pKa = 4.71EE43 pKa = 4.04IDD45 pKa = 3.42QLYY48 pKa = 10.69SDD50 pKa = 3.95EE51 pKa = 4.16QSRR54 pKa = 11.84LLSLSLLGDD63 pKa = 4.16LTCDD67 pKa = 2.95GCTII71 pKa = 4.25

Molecular weight: 8.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0FIN3|B0FIN3_BPE32 Uncharacterized protein OS=Escherichia phage Phieco32 OX=490103 GN=phi32_71 PE=4 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84PITMRR7 pKa = 11.84QFWLAKK13 pKa = 9.85IKK15 pKa = 9.25WASGGMTMTALPTRR29 pKa = 11.84QQARR33 pKa = 11.84DD34 pKa = 3.67FKK36 pKa = 11.32KK37 pKa = 11.19SMFNMDD43 pKa = 4.28MIKK46 pKa = 10.4SISIHH51 pKa = 5.81KK52 pKa = 9.85ARR54 pKa = 11.84EE55 pKa = 3.84YY56 pKa = 10.87SSGRR60 pKa = 11.84VYY62 pKa = 10.76ISGKK66 pKa = 7.19VHH68 pKa = 5.8YY69 pKa = 10.08

Molecular weight: 8.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

128

0

128

23545

35

1473

183.9

20.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.823 ± 0.499

1.227 ± 0.164

6.146 ± 0.196

6.94 ± 0.411

3.848 ± 0.161

7.254 ± 0.36

1.737 ± 0.159

5.691 ± 0.174

6.282 ± 0.297

7.386 ± 0.211

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.841 ± 0.165

5.67 ± 0.203

3.627 ± 0.168

4.175 ± 0.332

5.139 ± 0.271

6.795 ± 0.274

5.725 ± 0.311

6.791 ± 0.259

1.461 ± 0.097

3.44 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski