Amino acid dipepetide frequency for Escherichia phage Phieco32 (Escherichia coli phage phi32)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.305AlaAla: 7.305 ± 0.99
0.722AlaCys: 0.722 ± 0.186
5.139AlaAsp: 5.139 ± 0.49
4.8AlaGlu: 4.8 ± 0.786
2.888AlaPhe: 2.888 ± 0.326
5.394AlaGly: 5.394 ± 0.63
1.189AlaHis: 1.189 ± 0.227
4.29AlaIle: 4.29 ± 0.468
5.054AlaLys: 5.054 ± 0.827
7.221AlaLeu: 7.221 ± 0.536
2.209AlaMet: 2.209 ± 0.301
4.205AlaAsn: 4.205 ± 0.466
3.058AlaPro: 3.058 ± 0.359
4.035AlaGln: 4.035 ± 0.677
3.993AlaArg: 3.993 ± 0.611
5.054AlaSer: 5.054 ± 0.542
3.865AlaThr: 3.865 ± 0.47
4.672AlaVal: 4.672 ± 0.388
1.147AlaTrp: 1.147 ± 0.206
1.996AlaTyr: 1.996 ± 0.322
0.0AlaXaa: 0.0 ± 0.0
Cys
0.722CysAla: 0.722 ± 0.21
0.17CysCys: 0.17 ± 0.095
0.382CysAsp: 0.382 ± 0.142
0.765CysGlu: 0.765 ± 0.189
0.425CysPhe: 0.425 ± 0.138
1.062CysGly: 1.062 ± 0.215
0.255CysHis: 0.255 ± 0.099
0.765CysIle: 0.765 ± 0.176
1.019CysLys: 1.019 ± 0.208
1.189CysLeu: 1.189 ± 0.228
0.425CysMet: 0.425 ± 0.149
0.467CysAsn: 0.467 ± 0.147
0.467CysPro: 0.467 ± 0.144
0.34CysGln: 0.34 ± 0.124
0.552CysArg: 0.552 ± 0.147
1.019CysSer: 1.019 ± 0.223
0.552CysThr: 0.552 ± 0.198
1.019CysVal: 1.019 ± 0.249
0.127CysTrp: 0.127 ± 0.071
0.552CysTyr: 0.552 ± 0.151
0.0CysXaa: 0.0 ± 0.0
Asp
5.819AspAla: 5.819 ± 0.543
0.637AspCys: 0.637 ± 0.149
2.931AspAsp: 2.931 ± 0.364
4.927AspGlu: 4.927 ± 0.509
2.081AspPhe: 2.081 ± 0.299
4.842AspGly: 4.842 ± 0.626
0.934AspHis: 0.934 ± 0.244
4.247AspIle: 4.247 ± 0.431
3.313AspLys: 3.313 ± 0.359
4.63AspLeu: 4.63 ± 0.462
2.506AspMet: 2.506 ± 0.352
3.61AspAsn: 3.61 ± 0.387
2.294AspPro: 2.294 ± 0.366
1.954AspGln: 1.954 ± 0.256
2.633AspArg: 2.633 ± 0.315
3.738AspSer: 3.738 ± 0.404
3.186AspThr: 3.186 ± 0.381
3.993AspVal: 3.993 ± 0.358
0.807AspTrp: 0.807 ± 0.186
2.379AspTyr: 2.379 ± 0.325
0.0AspXaa: 0.0 ± 0.0
Glu
5.649GluAla: 5.649 ± 0.622
0.892GluCys: 0.892 ± 0.238
4.587GluAsp: 4.587 ± 0.528
7.178GluGlu: 7.178 ± 0.958
2.591GluPhe: 2.591 ± 0.354
4.162GluGly: 4.162 ± 0.384
1.487GluHis: 1.487 ± 0.262
3.865GluIle: 3.865 ± 0.307
4.375GluLys: 4.375 ± 0.531
4.63GluLeu: 4.63 ± 0.513
2.506GluMet: 2.506 ± 0.334
3.186GluAsn: 3.186 ± 0.372
1.741GluPro: 1.741 ± 0.341
3.016GluGln: 3.016 ± 0.416
3.695GluArg: 3.695 ± 0.459
4.035GluSer: 4.035 ± 0.376
3.186GluThr: 3.186 ± 0.361
5.054GluVal: 5.054 ± 0.494
1.232GluTrp: 1.232 ± 0.247
2.294GluTyr: 2.294 ± 0.339
0.0GluXaa: 0.0 ± 0.0
Phe
2.633PheAla: 2.633 ± 0.287
0.552PheCys: 0.552 ± 0.198
3.016PheAsp: 3.016 ± 0.478
2.379PheGlu: 2.379 ± 0.347
1.062PhePhe: 1.062 ± 0.235
2.421PheGly: 2.421 ± 0.315
0.637PheHis: 0.637 ± 0.149
2.633PheIle: 2.633 ± 0.345
2.336PheLys: 2.336 ± 0.354
3.143PheLeu: 3.143 ± 0.485
0.68PheMet: 0.68 ± 0.199
2.761PheAsn: 2.761 ± 0.287
1.232PhePro: 1.232 ± 0.257
1.656PheGln: 1.656 ± 0.304
2.124PheArg: 2.124 ± 0.257
2.209PheSer: 2.209 ± 0.328
2.336PheThr: 2.336 ± 0.341
2.336PheVal: 2.336 ± 0.299
0.425PheTrp: 0.425 ± 0.148
1.911PheTyr: 1.911 ± 0.337
0.0PheXaa: 0.0 ± 0.0
Gly
5.904GlyAla: 5.904 ± 0.815
1.189GlyCys: 1.189 ± 0.225
4.077GlyAsp: 4.077 ± 0.395
4.502GlyGlu: 4.502 ± 0.5
2.931GlyPhe: 2.931 ± 0.315
5.989GlyGly: 5.989 ± 0.75
1.019GlyHis: 1.019 ± 0.211
4.205GlyIle: 4.205 ± 0.418
4.672GlyLys: 4.672 ± 0.469
5.819GlyLeu: 5.819 ± 0.631
2.209GlyMet: 2.209 ± 0.293
3.823GlyAsn: 3.823 ± 0.473
0.849GlyPro: 0.849 ± 0.176
2.931GlyGln: 2.931 ± 0.474
3.653GlyArg: 3.653 ± 0.424
6.498GlySer: 6.498 ± 0.671
3.101GlyThr: 3.101 ± 0.502
5.522GlyVal: 5.522 ± 0.554
1.317GlyTrp: 1.317 ± 0.198
2.336GlyTyr: 2.336 ± 0.361
0.0GlyXaa: 0.0 ± 0.0
His
0.934HisAla: 0.934 ± 0.232
0.042HisCys: 0.042 ± 0.041
0.977HisAsp: 0.977 ± 0.214
1.019HisGlu: 1.019 ± 0.197
0.637HisPhe: 0.637 ± 0.153
1.274HisGly: 1.274 ± 0.239
0.297HisHis: 0.297 ± 0.103
0.934HisIle: 0.934 ± 0.184
1.232HisLys: 1.232 ± 0.254
1.359HisLeu: 1.359 ± 0.252
0.425HisMet: 0.425 ± 0.13
1.274HisAsn: 1.274 ± 0.246
0.637HisPro: 0.637 ± 0.176
0.51HisGln: 0.51 ± 0.126
1.189HisArg: 1.189 ± 0.23
1.402HisSer: 1.402 ± 0.313
0.934HisThr: 0.934 ± 0.203
1.189HisVal: 1.189 ± 0.265
0.255HisTrp: 0.255 ± 0.098
0.849HisTyr: 0.849 ± 0.215
0.0HisXaa: 0.0 ± 0.0
Ile
3.61IleAla: 3.61 ± 0.423
0.807IleCys: 0.807 ± 0.199
4.162IleAsp: 4.162 ± 0.395
3.823IleGlu: 3.823 ± 0.458
1.869IlePhe: 1.869 ± 0.286
4.035IleGly: 4.035 ± 0.509
0.892IleHis: 0.892 ± 0.205
2.379IleIle: 2.379 ± 0.317
4.46IleLys: 4.46 ± 0.33
3.355IleLeu: 3.355 ± 0.387
1.232IleMet: 1.232 ± 0.231
3.738IleAsn: 3.738 ± 0.43
3.398IlePro: 3.398 ± 0.476
1.784IleGln: 1.784 ± 0.348
3.058IleArg: 3.058 ± 0.353
4.12IleSer: 4.12 ± 0.42
4.502IleThr: 4.502 ± 0.468
3.186IleVal: 3.186 ± 0.406
0.765IleTrp: 0.765 ± 0.19
1.741IleTyr: 1.741 ± 0.274
0.0IleXaa: 0.0 ± 0.0
Lys
5.224LysAla: 5.224 ± 0.779
0.722LysCys: 0.722 ± 0.186
4.375LysAsp: 4.375 ± 0.392
4.969LysGlu: 4.969 ± 0.49
2.251LysPhe: 2.251 ± 0.37
4.29LysGly: 4.29 ± 0.514
1.572LysHis: 1.572 ± 0.261
3.016LysIle: 3.016 ± 0.337
4.842LysLys: 4.842 ± 0.531
4.205LysLeu: 4.205 ± 0.394
2.209LysMet: 2.209 ± 0.293
2.973LysAsn: 2.973 ± 0.319
1.911LysPro: 1.911 ± 0.332
2.633LysGln: 2.633 ± 0.319
2.761LysArg: 2.761 ± 0.416
3.568LysSer: 3.568 ± 0.389
4.035LysThr: 4.035 ± 0.467
3.823LysVal: 3.823 ± 0.401
1.104LysTrp: 1.104 ± 0.23
2.336LysTyr: 2.336 ± 0.359
0.0LysXaa: 0.0 ± 0.0
Leu
5.649LeuAla: 5.649 ± 0.509
0.977LeuCys: 0.977 ± 0.194
4.162LeuAsp: 4.162 ± 0.419
5.904LeuGlu: 5.904 ± 0.524
2.888LeuPhe: 2.888 ± 0.351
5.522LeuGly: 5.522 ± 0.647
1.104LeuHis: 1.104 ± 0.232
3.61LeuIle: 3.61 ± 0.432
4.035LeuLys: 4.035 ± 0.445
4.884LeuLeu: 4.884 ± 0.521
2.506LeuMet: 2.506 ± 0.235
4.12LeuAsn: 4.12 ± 0.504
3.27LeuPro: 3.27 ± 0.313
3.313LeuGln: 3.313 ± 0.404
4.077LeuArg: 4.077 ± 0.495
5.607LeuSer: 5.607 ± 0.621
4.247LeuThr: 4.247 ± 0.46
5.224LeuVal: 5.224 ± 0.5
0.51LeuTrp: 0.51 ± 0.149
2.251LeuTyr: 2.251 ± 0.301
0.0LeuXaa: 0.0 ± 0.0
Met
2.124MetAla: 2.124 ± 0.258
0.425MetCys: 0.425 ± 0.136
1.444MetAsp: 1.444 ± 0.221
2.166MetGlu: 2.166 ± 0.311
1.274MetPhe: 1.274 ± 0.235
1.402MetGly: 1.402 ± 0.228
0.51MetHis: 0.51 ± 0.155
1.741MetIle: 1.741 ± 0.231
2.251MetLys: 2.251 ± 0.346
1.741MetLeu: 1.741 ± 0.326
0.849MetMet: 0.849 ± 0.195
1.741MetAsn: 1.741 ± 0.242
0.892MetPro: 0.892 ± 0.184
0.849MetGln: 0.849 ± 0.182
1.784MetArg: 1.784 ± 0.263
2.548MetSer: 2.548 ± 0.339
2.166MetThr: 2.166 ± 0.297
1.444MetVal: 1.444 ± 0.292
0.34MetTrp: 0.34 ± 0.138
0.722MetTyr: 0.722 ± 0.177
0.0MetXaa: 0.0 ± 0.0
Asn
4.587AsnAla: 4.587 ± 0.413
0.297AsnCys: 0.297 ± 0.123
2.463AsnAsp: 2.463 ± 0.293
2.548AsnGlu: 2.548 ± 0.354
2.761AsnPhe: 2.761 ± 0.387
5.182AsnGly: 5.182 ± 0.679
0.807AsnHis: 0.807 ± 0.187
3.483AsnIle: 3.483 ± 0.359
3.228AsnLys: 3.228 ± 0.338
4.672AsnLeu: 4.672 ± 0.471
1.402AsnMet: 1.402 ± 0.252
3.44AsnAsn: 3.44 ± 0.446
2.591AsnPro: 2.591 ± 0.381
2.846AsnGln: 2.846 ± 0.429
3.016AsnArg: 3.016 ± 0.346
3.653AsnSer: 3.653 ± 0.424
3.483AsnThr: 3.483 ± 0.388
3.525AsnVal: 3.525 ± 0.409
0.849AsnTrp: 0.849 ± 0.189
1.869AsnTyr: 1.869 ± 0.267
0.0AsnXaa: 0.0 ± 0.0
Pro
2.846ProAla: 2.846 ± 0.3
0.425ProCys: 0.425 ± 0.122
2.676ProAsp: 2.676 ± 0.313
2.633ProGlu: 2.633 ± 0.305
1.699ProPhe: 1.699 ± 0.254
1.614ProGly: 1.614 ± 0.272
0.765ProHis: 0.765 ± 0.21
2.081ProIle: 2.081 ± 0.228
2.379ProLys: 2.379 ± 0.372
2.761ProLeu: 2.761 ± 0.417
0.807ProMet: 0.807 ± 0.183
1.614ProAsn: 1.614 ± 0.275
1.359ProPro: 1.359 ± 0.268
1.317ProGln: 1.317 ± 0.218
1.147ProArg: 1.147 ± 0.234
2.846ProSer: 2.846 ± 0.376
2.548ProThr: 2.548 ± 0.365
3.143ProVal: 3.143 ± 0.328
0.51ProTrp: 0.51 ± 0.131
1.104ProTyr: 1.104 ± 0.177
0.0ProXaa: 0.0 ± 0.0
Gln
4.12GlnAla: 4.12 ± 0.644
0.849GlnCys: 0.849 ± 0.213
2.379GlnAsp: 2.379 ± 0.394
2.506GlnGlu: 2.506 ± 0.397
1.317GlnPhe: 1.317 ± 0.217
2.463GlnGly: 2.463 ± 0.405
0.34GlnHis: 0.34 ± 0.127
2.336GlnIle: 2.336 ± 0.319
2.718GlnLys: 2.718 ± 0.39
2.676GlnLeu: 2.676 ± 0.454
0.934GlnMet: 0.934 ± 0.203
2.761GlnAsn: 2.761 ± 0.474
1.189GlnPro: 1.189 ± 0.251
3.101GlnGln: 3.101 ± 0.762
2.633GlnArg: 2.633 ± 0.488
2.209GlnSer: 2.209 ± 0.287
2.166GlnThr: 2.166 ± 0.273
2.761GlnVal: 2.761 ± 0.362
0.765GlnTrp: 0.765 ± 0.192
1.529GlnTyr: 1.529 ± 0.236
0.0GlnXaa: 0.0 ± 0.0
Arg
3.653ArgAla: 3.653 ± 0.619
0.722ArgCys: 0.722 ± 0.176
3.653ArgAsp: 3.653 ± 0.442
3.525ArgGlu: 3.525 ± 0.511
2.209ArgPhe: 2.209 ± 0.306
3.101ArgGly: 3.101 ± 0.393
0.977ArgHis: 0.977 ± 0.215
2.973ArgIle: 2.973 ± 0.451
3.016ArgLys: 3.016 ± 0.416
3.568ArgLeu: 3.568 ± 0.341
1.062ArgMet: 1.062 ± 0.208
2.548ArgAsn: 2.548 ± 0.339
1.699ArgPro: 1.699 ± 0.213
2.166ArgGln: 2.166 ± 0.335
3.186ArgArg: 3.186 ± 0.49
3.823ArgSer: 3.823 ± 0.411
2.931ArgThr: 2.931 ± 0.355
3.908ArgVal: 3.908 ± 0.488
0.849ArgTrp: 0.849 ± 0.209
1.826ArgTyr: 1.826 ± 0.269
0.0ArgXaa: 0.0 ± 0.0
Ser
3.95SerAla: 3.95 ± 0.418
0.807SerCys: 0.807 ± 0.164
4.035SerAsp: 4.035 ± 0.432
3.95SerGlu: 3.95 ± 0.327
2.421SerPhe: 2.421 ± 0.358
6.541SerGly: 6.541 ± 0.737
1.317SerHis: 1.317 ± 0.245
3.95SerIle: 3.95 ± 0.479
3.568SerLys: 3.568 ± 0.407
5.776SerLeu: 5.776 ± 0.43
1.911SerMet: 1.911 ± 0.28
4.46SerAsn: 4.46 ± 0.584
3.101SerPro: 3.101 ± 0.356
2.591SerGln: 2.591 ± 0.335
3.44SerArg: 3.44 ± 0.346
4.205SerSer: 4.205 ± 0.456
3.993SerThr: 3.993 ± 0.421
4.332SerVal: 4.332 ± 0.512
1.104SerTrp: 1.104 ± 0.186
2.506SerTyr: 2.506 ± 0.349
0.0SerXaa: 0.0 ± 0.0
Thr
4.63ThrAla: 4.63 ± 0.475
0.467ThrCys: 0.467 ± 0.15
2.803ThrAsp: 2.803 ± 0.272
3.483ThrGlu: 3.483 ± 0.331
2.294ThrPhe: 2.294 ± 0.379
5.522ThrGly: 5.522 ± 0.668
1.062ThrHis: 1.062 ± 0.219
3.313ThrIle: 3.313 ± 0.496
3.355ThrLys: 3.355 ± 0.303
4.8ThrLeu: 4.8 ± 0.475
1.062ThrMet: 1.062 ± 0.226
3.101ThrAsn: 3.101 ± 0.335
2.846ThrPro: 2.846 ± 0.35
2.081ThrGln: 2.081 ± 0.309
2.081ThrArg: 2.081 ± 0.319
3.313ThrSer: 3.313 ± 0.401
2.931ThrThr: 2.931 ± 0.48
4.842ThrVal: 4.842 ± 0.516
0.934ThrTrp: 0.934 ± 0.239
2.336ThrTyr: 2.336 ± 0.343
0.0ThrXaa: 0.0 ± 0.0
Val
5.437ValAla: 5.437 ± 0.442
0.722ValCys: 0.722 ± 0.201
4.672ValAsp: 4.672 ± 0.414
4.757ValGlu: 4.757 ± 0.456
3.101ValPhe: 3.101 ± 0.373
4.63ValGly: 4.63 ± 0.378
1.189ValHis: 1.189 ± 0.197
3.738ValIle: 3.738 ± 0.427
4.247ValLys: 4.247 ± 0.457
4.332ValLeu: 4.332 ± 0.435
1.614ValMet: 1.614 ± 0.27
4.077ValAsn: 4.077 ± 0.4
2.039ValPro: 2.039 ± 0.351
2.463ValGln: 2.463 ± 0.357
3.398ValArg: 3.398 ± 0.397
4.417ValSer: 4.417 ± 0.665
4.545ValThr: 4.545 ± 0.723
5.182ValVal: 5.182 ± 0.651
1.019ValTrp: 1.019 ± 0.236
2.336ValTyr: 2.336 ± 0.352
0.0ValXaa: 0.0 ± 0.0
Trp
1.019TrpAla: 1.019 ± 0.208
0.255TrpCys: 0.255 ± 0.123
1.274TrpAsp: 1.274 ± 0.233
0.722TrpGlu: 0.722 ± 0.152
0.68TrpPhe: 0.68 ± 0.156
0.977TrpGly: 0.977 ± 0.231
0.34TrpHis: 0.34 ± 0.117
1.147TrpIle: 1.147 ± 0.217
0.68TrpLys: 0.68 ± 0.17
0.68TrpLeu: 0.68 ± 0.181
0.807TrpMet: 0.807 ± 0.206
1.019TrpAsn: 1.019 ± 0.194
0.34TrpPro: 0.34 ± 0.121
0.467TrpGln: 0.467 ± 0.148
0.849TrpArg: 0.849 ± 0.176
0.977TrpSer: 0.977 ± 0.258
1.104TrpThr: 1.104 ± 0.181
0.765TrpVal: 0.765 ± 0.174
0.127TrpTrp: 0.127 ± 0.097
0.382TrpTyr: 0.382 ± 0.133
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.421TyrAla: 2.421 ± 0.266
0.595TyrCys: 0.595 ± 0.167
2.251TyrAsp: 2.251 ± 0.362
2.846TyrGlu: 2.846 ± 0.362
1.104TyrPhe: 1.104 ± 0.22
2.124TyrGly: 2.124 ± 0.281
0.68TyrHis: 0.68 ± 0.188
2.209TyrIle: 2.209 ± 0.289
2.039TyrLys: 2.039 ± 0.275
2.421TyrLeu: 2.421 ± 0.292
1.019TyrMet: 1.019 ± 0.203
1.784TyrAsn: 1.784 ± 0.217
1.359TyrPro: 1.359 ± 0.253
1.699TyrGln: 1.699 ± 0.271
2.124TyrArg: 2.124 ± 0.415
2.718TyrSer: 2.718 ± 0.279
1.444TyrThr: 1.444 ± 0.199
1.996TyrVal: 1.996 ± 0.315
0.425TyrTrp: 0.425 ± 0.156
1.147TyrTyr: 1.147 ± 0.26
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 128 proteins (23545 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski