Alkalihalobacillus krulwichiae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4515 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9MG23|A0A1X9MG23_9BACI Uncharacterized protein OS=Alkalihalobacillus krulwichiae OX=199441 GN=BkAM31D_05740 PE=4 SV=1
MM1 pKa = 7.49LKK3 pKa = 10.21KK4 pKa = 10.6LVGITSAVALTLGLAACGGEE24 pKa = 4.3EE25 pKa = 3.96TSGTSEE31 pKa = 3.91TSSVGEE37 pKa = 4.15QFDD40 pKa = 3.73HH41 pKa = 6.71TIVGIDD47 pKa = 3.43PGAGIMNLTINEE59 pKa = 4.2VVPEE63 pKa = 4.1YY64 pKa = 11.04GLEE67 pKa = 3.77DD68 pKa = 3.45WNIVEE73 pKa = 4.42GSGAAMTAALKK84 pKa = 10.25RR85 pKa = 11.84AYY87 pKa = 10.31DD88 pKa = 3.67NEE90 pKa = 3.97EE91 pKa = 4.4PIIVTGWSPHH101 pKa = 4.11WKK103 pKa = 9.57FAAFDD108 pKa = 4.23LKK110 pKa = 11.34YY111 pKa = 10.73LDD113 pKa = 4.76DD114 pKa = 4.69PKK116 pKa = 11.17GVYY119 pKa = 10.09GGAEE123 pKa = 4.16DD124 pKa = 3.42VHH126 pKa = 6.36TIARR130 pKa = 11.84QGLQEE135 pKa = 4.34DD136 pKa = 4.19HH137 pKa = 7.25PEE139 pKa = 4.64GYY141 pKa = 10.07QLLDD145 pKa = 3.29QFNWGPEE152 pKa = 3.88HH153 pKa = 7.27LEE155 pKa = 3.96TVMNLIQEE163 pKa = 4.49GQNPSDD169 pKa = 5.3AAAQWASEE177 pKa = 3.92NEE179 pKa = 4.21EE180 pKa = 4.45LVSTWTEE187 pKa = 3.87GVNEE191 pKa = 3.97VDD193 pKa = 4.72GEE195 pKa = 4.41EE196 pKa = 4.02LTLLYY201 pKa = 10.25VAWDD205 pKa = 3.66DD206 pKa = 4.56VIASSHH212 pKa = 4.08VVEE215 pKa = 4.78YY216 pKa = 10.51ALEE219 pKa = 4.58SIGFDD224 pKa = 3.06VDD226 pKa = 4.45LVQVDD231 pKa = 5.06AGPMWAGIASGSGDD245 pKa = 3.39AMVGAWLPTTHH256 pKa = 7.05ADD258 pKa = 3.39YY259 pKa = 10.49YY260 pKa = 11.46AEE262 pKa = 4.22YY263 pKa = 9.93EE264 pKa = 4.39GQFEE268 pKa = 4.65DD269 pKa = 4.92LGSNLTGTKK278 pKa = 10.19LGLVVPAYY286 pKa = 9.7MDD288 pKa = 3.44VEE290 pKa = 4.59SIEE293 pKa = 4.6DD294 pKa = 3.5LVEE297 pKa = 3.84

Molecular weight: 31.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9MHL7|A0A1X9MHL7_9BACI DUF5668 domain-containing protein OS=Alkalihalobacillus krulwichiae OX=199441 GN=BkAM31D_18215 PE=4 SV=1
MM1 pKa = 7.71GKK3 pKa = 8.0PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.34RR13 pKa = 11.84KK14 pKa = 8.16KK15 pKa = 9.1NHH17 pKa = 4.74GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 10.19NGRR29 pKa = 11.84KK30 pKa = 8.49VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4515

0

4515

1230270

29

1604

272.5

30.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.928 ± 0.039

0.731 ± 0.01

4.968 ± 0.029

7.855 ± 0.045

4.506 ± 0.032

6.752 ± 0.038

2.112 ± 0.022

7.785 ± 0.037

6.524 ± 0.035

9.973 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.75 ± 0.017

4.266 ± 0.023

3.541 ± 0.021

3.955 ± 0.027

4.263 ± 0.026

5.942 ± 0.027

5.466 ± 0.024

7.312 ± 0.027

1.008 ± 0.014

3.36 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski