Besnoitia besnoiti

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia; Eucoccidiorida; Eimeriorina; Sarcocystidae; Besnoitia

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8036 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A9MMI2|A0A2A9MMI2_9APIC Uncharacterized protein OS=Besnoitia besnoiti OX=94643 GN=BESB_008960 PE=4 SV=1
MM1 pKa = 7.27EE2 pKa = 5.58RR3 pKa = 11.84SNPRR7 pKa = 11.84LEE9 pKa = 4.44RR10 pKa = 11.84SNPDD14 pKa = 2.66WRR16 pKa = 11.84DD17 pKa = 3.21PAPVWRR23 pKa = 11.84SRR25 pKa = 11.84APDD28 pKa = 3.13WRR30 pKa = 11.84SRR32 pKa = 11.84APDD35 pKa = 3.15WRR37 pKa = 11.84NPAPHH42 pKa = 6.69WRR44 pKa = 11.84DD45 pKa = 3.34GTPHH49 pKa = 5.86WRR51 pKa = 11.84NRR53 pKa = 11.84APTGEE58 pKa = 4.09VEE60 pKa = 4.17PPYY63 pKa = 11.23GEE65 pKa = 3.96IQPPTGEE72 pKa = 4.05VEE74 pKa = 4.0PPYY77 pKa = 11.21GEE79 pKa = 4.44VEE81 pKa = 4.2PPTGEE86 pKa = 3.58IEE88 pKa = 4.05PPYY91 pKa = 10.79EE92 pKa = 4.17EE93 pKa = 5.01VEE95 pKa = 4.11PPYY98 pKa = 11.3GEE100 pKa = 4.44VEE102 pKa = 4.21PPTGEE107 pKa = 3.64IQPPYY112 pKa = 11.03GEE114 pKa = 4.47IEE116 pKa = 3.96PPYY119 pKa = 10.99GEE121 pKa = 4.52VEE123 pKa = 4.21PPTGEE128 pKa = 3.67IQPPNGEE135 pKa = 4.18IEE137 pKa = 4.28PPTGEE142 pKa = 3.98VEE144 pKa = 4.07PPYY147 pKa = 11.23GEE149 pKa = 3.96IQPPTGEE156 pKa = 4.05IQPPTGEE163 pKa = 3.82IQPPYY168 pKa = 10.95GEE170 pKa = 4.55VEE172 pKa = 4.13PPTGEE177 pKa = 4.08VEE179 pKa = 4.22PPTGEE184 pKa = 3.72IQLPTGEE191 pKa = 4.6MEE193 pKa = 4.53PPTGEE198 pKa = 3.88IEE200 pKa = 4.25PPTGEE205 pKa = 3.98VEE207 pKa = 4.05PPYY210 pKa = 11.2GEE212 pKa = 4.49VEE214 pKa = 4.16PTTGEE219 pKa = 3.67IQPPYY224 pKa = 11.03GEE226 pKa = 4.47IEE228 pKa = 3.96PPYY231 pKa = 10.99GEE233 pKa = 4.52VEE235 pKa = 4.21PPTGEE240 pKa = 3.64IQPPYY245 pKa = 11.03GEE247 pKa = 4.47IEE249 pKa = 3.96PPYY252 pKa = 10.99GEE254 pKa = 4.52VEE256 pKa = 4.21PPTGEE261 pKa = 3.64IQPPYY266 pKa = 10.95GEE268 pKa = 4.55VEE270 pKa = 4.13PPTGEE275 pKa = 3.93VEE277 pKa = 4.05PPYY280 pKa = 11.23GEE282 pKa = 4.37VEE284 pKa = 4.02PPYY287 pKa = 11.22GEE289 pKa = 3.96IQPPTGEE296 pKa = 3.91IQTPDD301 pKa = 3.07WRR303 pKa = 11.84DD304 pKa = 3.26PAPP307 pKa = 3.81

Molecular weight: 33.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A9MI01|A0A2A9MI01_9APIC Ubiquitin carboxyl-terminal hydrolase OS=Besnoitia besnoiti OX=94643 GN=BESB_055320 PE=4 SV=1
MM1 pKa = 6.41VAARR5 pKa = 11.84AEE7 pKa = 4.11EE8 pKa = 4.03RR9 pKa = 11.84RR10 pKa = 11.84VCSPPSSAEE19 pKa = 4.0SSSSLSAFSEE29 pKa = 4.28GRR31 pKa = 11.84GRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84PRR37 pKa = 11.84EE38 pKa = 3.88KK39 pKa = 10.91APATAALDD47 pKa = 3.71RR48 pKa = 11.84FGLFSQTQSPWTTLAAPAFCTTGSVSRR75 pKa = 11.84YY76 pKa = 6.65EE77 pKa = 4.03QLVAAIKK84 pKa = 9.77EE85 pKa = 4.04KK86 pKa = 10.99HH87 pKa = 6.23KK88 pKa = 10.86GRR90 pKa = 11.84KK91 pKa = 6.41TKK93 pKa = 9.51EE94 pKa = 3.43KK95 pKa = 10.43RR96 pKa = 11.84RR97 pKa = 11.84ISEE100 pKa = 4.0GLLLFLAAVTHH111 pKa = 6.72GGTAALSRR119 pKa = 11.84LLLAPLDD126 pKa = 3.55QEE128 pKa = 4.6KK129 pKa = 10.39IIRR132 pKa = 11.84QLSLARR138 pKa = 11.84LPSLSPSSSPWSPSPQGSAVCASPFLVSEE167 pKa = 4.39SASPSSSAPVRR178 pKa = 11.84ARR180 pKa = 11.84RR181 pKa = 11.84PQSSSALSSGPPSSASPPSSSVSFSAARR209 pKa = 11.84ARR211 pKa = 11.84ASAASSAPNSSVKK224 pKa = 10.3PPSAPAGASIRR235 pKa = 11.84PSPSANSAAAFPTSGLPSASSLPPSRR261 pKa = 11.84WLTVSSFWLGCSAPICAAIGGSWIRR286 pKa = 11.84LALYY290 pKa = 9.97QRR292 pKa = 11.84TRR294 pKa = 11.84LFFTDD299 pKa = 4.05GEE301 pKa = 4.5EE302 pKa = 4.43TYY304 pKa = 11.58SNAEE308 pKa = 3.6RR309 pKa = 11.84FFRR312 pKa = 11.84NMGCICFSAFVALAVCYY329 pKa = 9.47PLDD332 pKa = 3.7VAHH335 pKa = 6.72TAMCVIAASARR346 pKa = 11.84QSGRR350 pKa = 11.84VISSLDD356 pKa = 3.24AFSAFSATSAPQGARR371 pKa = 11.84RR372 pKa = 11.84EE373 pKa = 4.13ASVSFLSRR381 pKa = 11.84RR382 pKa = 11.84FASAKK387 pKa = 9.78KK388 pKa = 10.24RR389 pKa = 11.84LGAQLTGTVTTGSARR404 pKa = 11.84AEE406 pKa = 4.39TVDD409 pKa = 3.32GEE411 pKa = 3.99ARR413 pKa = 11.84QRR415 pKa = 11.84HH416 pKa = 4.91ASARR420 pKa = 11.84AVSPSHH426 pKa = 6.36IVWRR430 pKa = 11.84LYY432 pKa = 10.25KK433 pKa = 10.32QGGVRR438 pKa = 11.84ALYY441 pKa = 10.58CGFGLCALTLVPFTLASAFLQLKK464 pKa = 8.29FQSLLLSLQQQEE476 pKa = 4.73GAVAAHH482 pKa = 6.98WPRR485 pKa = 11.84LFASRR490 pKa = 11.84NGDD493 pKa = 3.62LPLACDD499 pKa = 3.52APKK502 pKa = 11.01GDD504 pKa = 3.58GRR506 pKa = 11.84GADD509 pKa = 3.84EE510 pKa = 4.58EE511 pKa = 4.74EE512 pKa = 3.98EE513 pKa = 4.22DD514 pKa = 4.02ARR516 pKa = 11.84GLSRR520 pKa = 11.84EE521 pKa = 4.14KK522 pKa = 10.58EE523 pKa = 3.95RR524 pKa = 11.84SPVADD529 pKa = 3.85EE530 pKa = 3.91TATAGDD536 pKa = 3.22QGAAPRR542 pKa = 11.84RR543 pKa = 11.84RR544 pKa = 11.84KK545 pKa = 8.52TGGKK549 pKa = 8.22RR550 pKa = 11.84QAVRR554 pKa = 11.84GDD556 pKa = 3.22ADD558 pKa = 3.47EE559 pKa = 4.23TNRR562 pKa = 11.84AGEE565 pKa = 4.25SASEE569 pKa = 4.36DD570 pKa = 3.49GRR572 pKa = 11.84EE573 pKa = 3.79TTYY576 pKa = 10.73PRR578 pKa = 11.84GRR580 pKa = 11.84GGSAFRR586 pKa = 11.84QARR589 pKa = 11.84GEE591 pKa = 4.16IAHH594 pKa = 6.5SPQTDD599 pKa = 2.98SFEE602 pKa = 4.58RR603 pKa = 11.84KK604 pKa = 7.72RR605 pKa = 11.84HH606 pKa = 4.78EE607 pKa = 4.17EE608 pKa = 3.89RR609 pKa = 11.84EE610 pKa = 4.14EE611 pKa = 3.99TLGSSRR617 pKa = 11.84WKK619 pKa = 10.47AAATAAFAAGLVAQLATYY637 pKa = 9.53PLDD640 pKa = 3.98SIRR643 pKa = 11.84RR644 pKa = 11.84RR645 pKa = 11.84QQFATISSLLFPHH658 pKa = 6.84GVAAPSRR665 pKa = 11.84VQPPASPMSVHH676 pKa = 6.47PPKK679 pKa = 10.06PVSGFRR685 pKa = 11.84RR686 pKa = 11.84FFVVPSSRR694 pKa = 11.84AWFSKK699 pKa = 9.33PHH701 pKa = 6.12GGLFRR706 pKa = 11.84GAGVLLVRR714 pKa = 11.84SVPEE718 pKa = 3.51CAIAVSVYY726 pKa = 10.42SFLMSKK732 pKa = 9.27MPSLAYY738 pKa = 10.08HH739 pKa = 6.93

Molecular weight: 78.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8036

0

8036

6780256

18

16347

843.7

90.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.892 ± 0.049

1.938 ± 0.015

4.759 ± 0.017

7.202 ± 0.03

3.182 ± 0.016

8.073 ± 0.028

2.089 ± 0.011

2.269 ± 0.02

3.59 ± 0.029

8.985 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.329 ± 0.01

1.978 ± 0.015

6.937 ± 0.029

3.803 ± 0.017

8.257 ± 0.035

10.167 ± 0.04

4.539 ± 0.016

5.514 ± 0.022

0.998 ± 0.006

1.498 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski