Actinomyces bovis
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2148 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2X0UBI8|A0A2X0UBI8_9ACTO Hypoxanthine phosphoribosyltransferase OS=Actinomyces bovis OX=1658 GN=hpt PE=3 SV=1
MM1 pKa = 6.47 TQYY4 pKa = 10.81 EE5 pKa = 4.48 AGLMVEE11 pKa = 5.29 DD12 pKa = 3.83 ISVAKK17 pKa = 10.3 FLLPTTLPEE26 pKa = 3.93 TWTGSAATACANALTQASKK45 pKa = 11.16 LLDD48 pKa = 3.47 QVTLSGGQASNSCLALDD65 pKa = 4.04 QVII68 pKa = 3.91
Molecular weight: 7.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.838
IPC2_protein 4.126
IPC_protein 3.846
Toseland 3.681
ProMoST 4.05
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.808
Rodwell 3.706
Grimsley 3.617
Solomon 3.795
Lehninger 3.757
Nozaki 4.012
DTASelect 4.126
Thurlkill 3.783
EMBOSS 3.821
Sillero 3.973
Patrickios 1.914
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.877
Protein with the highest isoelectric point:
>tr|A0A2X0UFL7|A0A2X0UFL7_9ACTO Uncharacterized protein OS=Actinomyces bovis OX=1658 GN=NCTC11535_02137 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.4 VHH17 pKa = 5.52 GFRR20 pKa = 11.84 KK21 pKa = 9.84 RR22 pKa = 11.84 MSSRR26 pKa = 11.84 AGRR29 pKa = 11.84 AVLASRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.61 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LAAA45 pKa = 4.44
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2148
0
2148
763328
29
3149
355.4
37.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.499 ± 0.088
0.827 ± 0.014
5.299 ± 0.042
5.82 ± 0.056
2.516 ± 0.032
8.712 ± 0.047
1.979 ± 0.018
3.658 ± 0.051
2.702 ± 0.048
10.491 ± 0.078
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.893 ± 0.023
2.175 ± 0.029
5.731 ± 0.047
3.622 ± 0.03
6.799 ± 0.055
6.019 ± 0.047
6.284 ± 0.055
8.551 ± 0.05
1.489 ± 0.023
1.934 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here