Corticibacter populi
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3638 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3M6QW52|A0A3M6QW52_9BURK Efflux RND transporter permease subunit OS=Corticibacter populi OX=1550736 GN=D8I35_09640 PE=4 SV=1
MM1 pKa = 7.27 TRR3 pKa = 11.84 PASLAVVLEE12 pKa = 4.4 GGLVQALLVQDD23 pKa = 4.4 WPAHH27 pKa = 5.36 VPLPCIAVVDD37 pKa = 4.23 YY38 pKa = 8.9 DD39 pKa = 4.38 TEE41 pKa = 4.33 GADD44 pKa = 4.55 DD45 pKa = 5.27 DD46 pKa = 5.4 EE47 pKa = 4.68 ITHH50 pKa = 6.28 FLIGGKK56 pKa = 9.02 PEE58 pKa = 3.95 EE59 pKa = 4.35 AVCRR63 pKa = 11.84 SDD65 pKa = 3.51 VPEE68 pKa = 4.37 VYY70 pKa = 10.12 EE71 pKa = 4.55 HH72 pKa = 6.58 LTDD75 pKa = 4.01 ALSPLAVLTALGDD88 pKa = 3.88 PPPDD92 pKa = 4.84 DD93 pKa = 5.59 DD94 pKa = 5.79 GEE96 pKa = 4.51 PPLALAQSVRR106 pKa = 11.84 QSILDD111 pKa = 3.51 LDD113 pKa = 3.77 ARR115 pKa = 11.84 INQSEE120 pKa = 4.18 QPPTGDD126 pKa = 4.7 DD127 pKa = 3.71 YY128 pKa = 12.11 NHH130 pKa = 7.14 LYY132 pKa = 10.66 VLANCGLIDD141 pKa = 3.72 VLKK144 pKa = 10.97 ALGDD148 pKa = 3.56 PTDD151 pKa = 3.89 FGEE154 pKa = 4.23
Molecular weight: 16.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.757
IPC_protein 3.757
Toseland 3.541
ProMoST 3.91
Dawson 3.757
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.579
Grimsley 3.452
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.126
Thurlkill 3.592
EMBOSS 3.719
Sillero 3.884
Patrickios 1.875
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|A0A3M6QNY4|A0A3M6QNY4_9BURK DUF4148 domain-containing protein OS=Corticibacter populi OX=1550736 GN=D8I35_12995 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.07 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.91 GFLVRR21 pKa = 11.84 MKK23 pKa = 9.7 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.6 RR41 pKa = 11.84 LAVAA45 pKa = 4.53
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3638
0
3638
1229857
37
3287
338.1
36.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.96 ± 0.053
0.864 ± 0.012
5.105 ± 0.03
5.221 ± 0.034
3.34 ± 0.025
8.303 ± 0.034
2.381 ± 0.021
4.53 ± 0.029
2.742 ± 0.037
11.095 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.309 ± 0.019
2.536 ± 0.024
5.371 ± 0.034
4.853 ± 0.036
6.935 ± 0.038
5.425 ± 0.033
5.002 ± 0.033
7.168 ± 0.035
1.586 ± 0.02
2.276 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here