Hymenobacter sp.
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2772 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A519Q9P0|A0A519Q9P0_9BACT Uncharacterized protein OS=Hymenobacter sp. OX=1898978 GN=EOO60_01165 PE=4 SV=1
MM1 pKa = 7.94 DD2 pKa = 4.12 VPATDD7 pKa = 5.94 ALLKK11 pKa = 9.72 WAADD15 pKa = 3.73 PQQQQAASIVFLDD28 pKa = 3.67 ADD30 pKa = 4.18 GGSSLEE36 pKa = 4.03 TLRR39 pKa = 11.84 LPGAYY44 pKa = 8.78 CVRR47 pKa = 11.84 YY48 pKa = 9.77 RR49 pKa = 11.84 EE50 pKa = 4.46 EE51 pKa = 3.92 FTQGDD56 pKa = 4.22 EE57 pKa = 4.14 QEE59 pKa = 4.35 GAYY62 pKa = 8.64 TCQLTLADD70 pKa = 3.98 PSGWTLQATSQQVPAEE86 pKa = 3.98 PAAASSTAA94 pKa = 3.21
Molecular weight: 9.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.859
IPC_protein 3.757
Toseland 3.567
ProMoST 3.935
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.478
Solomon 3.719
Lehninger 3.681
Nozaki 3.884
DTASelect 4.062
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.884
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|A0A519Q740|A0A519Q740_9BACT DUF4433 domain-containing protein OS=Hymenobacter sp. OX=1898978 GN=EOO60_03335 PE=4 SV=1
MM1 pKa = 7.39 LHH3 pKa = 6.61 VSWQKK8 pKa = 10.97 RR9 pKa = 11.84 KK10 pKa = 7.78 TLLKK14 pKa = 10.61 SITRR18 pKa = 11.84 LPRR21 pKa = 11.84 LPRR24 pKa = 11.84 LPRR27 pKa = 11.84 LPRR30 pKa = 11.84 LPRR33 pKa = 11.84 LPRR36 pKa = 11.84 LPRR39 pKa = 11.84 LPRR42 pKa = 11.84 LPRR45 pKa = 11.84 LPRR48 pKa = 11.84 LPRR51 pKa = 11.84 LPRR54 pKa = 11.84 LPRR57 pKa = 11.84 LPRR60 pKa = 11.84 LPP62 pKa = 3.91
Molecular weight: 7.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.519
IPC2_protein 11.403
IPC_protein 13.013
Toseland 13.173
ProMoST 13.671
Dawson 13.173
Bjellqvist 13.173
Wikipedia 13.656
Rodwell 12.749
Grimsley 13.217
Solomon 13.671
Lehninger 13.583
Nozaki 13.173
DTASelect 13.173
Thurlkill 13.173
EMBOSS 13.671
Sillero 13.173
Patrickios 12.486
IPC_peptide 13.685
IPC2_peptide 12.676
IPC2.peptide.svr19 9.309
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2772
0
2772
561870
20
1264
202.7
22.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.004 ± 0.064
0.854 ± 0.02
4.671 ± 0.034
5.108 ± 0.055
4.064 ± 0.039
6.984 ± 0.058
2.105 ± 0.027
4.502 ± 0.036
4.281 ± 0.048
11.132 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.838 ± 0.026
3.957 ± 0.06
4.939 ± 0.037
5.0 ± 0.038
5.687 ± 0.045
6.083 ± 0.044
6.76 ± 0.063
6.789 ± 0.041
1.351 ± 0.022
3.891 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here