Amino acid dipepetide frequency for Hymenobacter sp.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.239AlaAla: 11.239 ± 0.174
0.84AlaCys: 0.84 ± 0.042
4.987AlaAsp: 4.987 ± 0.094
5.359AlaGlu: 5.359 ± 0.108
3.581AlaPhe: 3.581 ± 0.083
7.5AlaGly: 7.5 ± 0.136
1.958AlaHis: 1.958 ± 0.067
4.385AlaIle: 4.385 ± 0.087
4.168AlaLys: 4.168 ± 0.098
10.567AlaLeu: 10.567 ± 0.158
1.887AlaMet: 1.887 ± 0.058
3.832AlaAsn: 3.832 ± 0.098
4.36AlaPro: 4.36 ± 0.107
5.507AlaGln: 5.507 ± 0.11
5.056AlaArg: 5.056 ± 0.108
6.085AlaSer: 6.085 ± 0.116
7.42AlaThr: 7.42 ± 0.123
6.347AlaVal: 6.347 ± 0.116
1.281AlaTrp: 1.281 ± 0.059
3.679AlaTyr: 3.679 ± 0.085
0.002AlaXaa: 0.002 ± 0.002
Cys
0.733CysAla: 0.733 ± 0.035
0.153CysCys: 0.153 ± 0.016
0.345CysAsp: 0.345 ± 0.023
0.367CysGlu: 0.367 ± 0.027
0.452CysPhe: 0.452 ± 0.032
0.58CysGly: 0.58 ± 0.03
0.306CysHis: 0.306 ± 0.031
0.376CysIle: 0.376 ± 0.027
0.215CysLys: 0.215 ± 0.021
1.075CysLeu: 1.075 ± 0.049
0.128CysMet: 0.128 ± 0.014
0.281CysAsn: 0.281 ± 0.024
0.409CysPro: 0.409 ± 0.032
0.53CysGln: 0.53 ± 0.03
0.537CysArg: 0.537 ± 0.031
0.566CysSer: 0.566 ± 0.035
0.488CysThr: 0.488 ± 0.031
0.53CysVal: 0.53 ± 0.032
0.13CysTrp: 0.13 ± 0.017
0.34CysTyr: 0.34 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
4.446AspAla: 4.446 ± 0.086
0.454AspCys: 0.454 ± 0.027
2.161AspAsp: 2.161 ± 0.065
2.714AspGlu: 2.714 ± 0.078
2.586AspPhe: 2.586 ± 0.071
3.378AspGly: 3.378 ± 0.091
0.824AspHis: 0.824 ± 0.043
2.223AspIle: 2.223 ± 0.07
2.422AspLys: 2.422 ± 0.078
4.994AspLeu: 4.994 ± 0.084
0.961AspMet: 0.961 ± 0.038
1.956AspAsn: 1.956 ± 0.068
2.184AspPro: 2.184 ± 0.062
1.881AspGln: 1.881 ± 0.058
2.202AspArg: 2.202 ± 0.059
2.609AspSer: 2.609 ± 0.064
2.565AspThr: 2.565 ± 0.074
3.015AspVal: 3.015 ± 0.077
0.817AspTrp: 0.817 ± 0.038
2.323AspTyr: 2.323 ± 0.069
0.0AspXaa: 0.0 ± 0.0
Glu
5.653GluAla: 5.653 ± 0.119
0.338GluCys: 0.338 ± 0.025
2.098GluAsp: 2.098 ± 0.066
3.011GluGlu: 3.011 ± 0.094
1.945GluPhe: 1.945 ± 0.069
2.78GluGly: 2.78 ± 0.072
1.143GluHis: 1.143 ± 0.052
2.408GluIle: 2.408 ± 0.066
2.572GluLys: 2.572 ± 0.074
6.416GluLeu: 6.416 ± 0.101
1.159GluMet: 1.159 ± 0.042
1.742GluAsn: 1.742 ± 0.056
2.041GluPro: 2.041 ± 0.062
2.949GluGln: 2.949 ± 0.08
3.253GluArg: 3.253 ± 0.08
2.323GluSer: 2.323 ± 0.067
2.504GluThr: 2.504 ± 0.065
4.045GluVal: 4.045 ± 0.08
0.737GluTrp: 0.737 ± 0.035
1.963GluTyr: 1.963 ± 0.063
0.0GluXaa: 0.0 ± 0.0
Phe
3.536PheAla: 3.536 ± 0.087
0.383PheCys: 0.383 ± 0.026
2.31PheAsp: 2.31 ± 0.056
2.143PheGlu: 2.143 ± 0.057
1.822PhePhe: 1.822 ± 0.076
3.018PheGly: 3.018 ± 0.084
0.755PheHis: 0.755 ± 0.037
2.02PheIle: 2.02 ± 0.058
1.598PheLys: 1.598 ± 0.056
3.967PheLeu: 3.967 ± 0.106
0.642PheMet: 0.642 ± 0.036
1.94PheAsn: 1.94 ± 0.066
1.566PhePro: 1.566 ± 0.062
1.474PheGln: 1.474 ± 0.048
2.162PheArg: 2.162 ± 0.066
2.972PheSer: 2.972 ± 0.072
3.282PheThr: 3.282 ± 0.086
2.689PheVal: 2.689 ± 0.084
0.657PheTrp: 0.657 ± 0.038
1.703PheTyr: 1.703 ± 0.061
0.0PheXaa: 0.0 ± 0.0
Gly
5.936GlyAla: 5.936 ± 0.121
0.644GlyCys: 0.644 ± 0.038
2.88GlyAsp: 2.88 ± 0.079
3.2GlyGlu: 3.2 ± 0.064
2.89GlyPhe: 2.89 ± 0.085
5.136GlyGly: 5.136 ± 0.123
1.456GlyHis: 1.456 ± 0.049
3.641GlyIle: 3.641 ± 0.092
3.408GlyLys: 3.408 ± 0.088
7.521GlyLeu: 7.521 ± 0.145
1.394GlyMet: 1.394 ± 0.058
3.056GlyAsn: 3.056 ± 0.101
2.609GlyPro: 2.609 ± 0.07
3.727GlyGln: 3.727 ± 0.081
3.949GlyArg: 3.949 ± 0.078
4.688GlySer: 4.688 ± 0.112
5.112GlyThr: 5.112 ± 0.157
4.522GlyVal: 4.522 ± 0.104
1.109GlyTrp: 1.109 ± 0.044
2.96GlyTyr: 2.96 ± 0.084
0.0GlyXaa: 0.0 ± 0.0
His
1.675HisAla: 1.675 ± 0.058
0.287HisCys: 0.287 ± 0.024
1.032HisAsp: 1.032 ± 0.044
1.086HisGlu: 1.086 ± 0.045
1.055HisPhe: 1.055 ± 0.045
1.207HisGly: 1.207 ± 0.047
0.573HisHis: 0.573 ± 0.038
0.913HisIle: 0.913 ± 0.041
0.84HisLys: 0.84 ± 0.042
2.614HisLeu: 2.614 ± 0.08
0.343HisMet: 0.343 ± 0.024
0.769HisAsn: 0.769 ± 0.042
1.189HisPro: 1.189 ± 0.051
1.128HisGln: 1.128 ± 0.043
1.201HisArg: 1.201 ± 0.049
1.278HisSer: 1.278 ± 0.048
1.219HisThr: 1.219 ± 0.052
1.159HisVal: 1.159 ± 0.043
0.438HisTrp: 0.438 ± 0.029
1.039HisTyr: 1.039 ± 0.046
0.0HisXaa: 0.0 ± 0.0
Ile
4.369IleAla: 4.369 ± 0.102
0.432IleCys: 0.432 ± 0.025
2.374IleAsp: 2.374 ± 0.072
2.723IleGlu: 2.723 ± 0.079
1.634IlePhe: 1.634 ± 0.057
3.414IleGly: 3.414 ± 0.094
0.829IleHis: 0.829 ± 0.04
2.451IleIle: 2.451 ± 0.067
2.091IleLys: 2.091 ± 0.068
3.91IleLeu: 3.91 ± 0.087
0.73IleMet: 0.73 ± 0.036
1.984IleAsn: 1.984 ± 0.069
2.267IlePro: 2.267 ± 0.064
1.806IleGln: 1.806 ± 0.049
2.574IleArg: 2.574 ± 0.072
3.23IleSer: 3.23 ± 0.08
3.204IleThr: 3.204 ± 0.08
2.91IleVal: 2.91 ± 0.091
0.482IleTrp: 0.482 ± 0.03
1.602IleTyr: 1.602 ± 0.053
0.0IleXaa: 0.0 ± 0.0
Lys
4.232LysAla: 4.232 ± 0.103
0.287LysCys: 0.287 ± 0.023
1.933LysAsp: 1.933 ± 0.061
2.429LysGlu: 2.429 ± 0.08
1.447LysPhe: 1.447 ± 0.053
2.894LysGly: 2.894 ± 0.071
0.865LysHis: 0.865 ± 0.042
1.931LysIle: 1.931 ± 0.07
2.367LysLys: 2.367 ± 0.08
4.629LysLeu: 4.629 ± 0.092
1.036LysMet: 1.036 ± 0.051
1.648LysAsn: 1.648 ± 0.063
2.2LysPro: 2.2 ± 0.063
2.371LysGln: 2.371 ± 0.068
2.629LysArg: 2.629 ± 0.074
2.371LysSer: 2.371 ± 0.069
2.517LysThr: 2.517 ± 0.075
2.937LysVal: 2.937 ± 0.08
0.477LysTrp: 0.477 ± 0.03
1.611LysTyr: 1.611 ± 0.055
0.0LysXaa: 0.0 ± 0.0
Leu
12.142LeuAla: 12.142 ± 0.19
0.901LeuCys: 0.901 ± 0.045
5.405LeuAsp: 5.405 ± 0.103
5.083LeuGlu: 5.083 ± 0.114
4.35LeuPhe: 4.35 ± 0.094
7.379LeuGly: 7.379 ± 0.121
2.556LeuHis: 2.556 ± 0.073
4.255LeuIle: 4.255 ± 0.083
4.159LeuLys: 4.159 ± 0.107
14.211LeuLeu: 14.211 ± 0.229
1.773LeuMet: 1.773 ± 0.059
4.17LeuAsn: 4.17 ± 0.093
6.274LeuPro: 6.274 ± 0.133
4.485LeuGln: 4.485 ± 0.092
7.16LeuArg: 7.16 ± 0.128
6.948LeuSer: 6.948 ± 0.106
7.835LeuThr: 7.835 ± 0.128
7.756LeuVal: 7.756 ± 0.119
1.041LeuTrp: 1.041 ± 0.056
3.435LeuTyr: 3.435 ± 0.08
0.002LeuXaa: 0.002 ± 0.002
Met
1.801MetAla: 1.801 ± 0.06
0.125MetCys: 0.125 ± 0.015
0.689MetAsp: 0.689 ± 0.039
0.925MetGlu: 0.925 ± 0.04
0.655MetPhe: 0.655 ± 0.037
1.192MetGly: 1.192 ± 0.046
0.436MetHis: 0.436 ± 0.032
0.637MetIle: 0.637 ± 0.034
1.132MetLys: 1.132 ± 0.049
2.056MetLeu: 2.056 ± 0.061
0.427MetMet: 0.427 ± 0.029
0.812MetAsn: 0.812 ± 0.04
1.216MetPro: 1.216 ± 0.042
1.08MetGln: 1.08 ± 0.044
1.239MetArg: 1.239 ± 0.055
1.178MetSer: 1.178 ± 0.048
1.087MetThr: 1.087 ± 0.039
1.025MetVal: 1.025 ± 0.042
0.185MetTrp: 0.185 ± 0.015
0.481MetTyr: 0.481 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.503AsnAla: 3.503 ± 0.101
0.361AsnCys: 0.361 ± 0.029
1.716AsnAsp: 1.716 ± 0.061
1.81AsnGlu: 1.81 ± 0.06
1.598AsnPhe: 1.598 ± 0.059
3.294AsnGly: 3.294 ± 0.116
0.737AsnHis: 0.737 ± 0.04
1.94AsnIle: 1.94 ± 0.06
1.71AsnLys: 1.71 ± 0.053
3.652AsnLeu: 3.652 ± 0.072
0.803AsnMet: 0.803 ± 0.039
1.956AsnAsn: 1.956 ± 0.094
2.399AsnPro: 2.399 ± 0.071
1.944AsnGln: 1.944 ± 0.064
2.138AsnArg: 2.138 ± 0.07
2.561AsnSer: 2.561 ± 0.079
2.766AsnThr: 2.766 ± 0.098
2.381AsnVal: 2.381 ± 0.074
0.618AsnTrp: 0.618 ± 0.035
1.689AsnTyr: 1.689 ± 0.06
0.0AsnXaa: 0.0 ± 0.0
Pro
6.172ProAla: 6.172 ± 0.119
0.304ProCys: 0.304 ± 0.026
2.768ProAsp: 2.768 ± 0.066
2.8ProGlu: 2.8 ± 0.073
1.846ProPhe: 1.846 ± 0.06
3.439ProGly: 3.439 ± 0.078
1.007ProHis: 1.007 ± 0.046
2.068ProIle: 2.068 ± 0.061
1.803ProLys: 1.803 ± 0.055
4.834ProLeu: 4.834 ± 0.103
0.865ProMet: 0.865 ± 0.04
2.043ProAsn: 2.043 ± 0.062
2.166ProPro: 2.166 ± 0.072
1.993ProGln: 1.993 ± 0.06
2.221ProArg: 2.221 ± 0.07
2.858ProSer: 2.858 ± 0.073
4.012ProThr: 4.012 ± 0.087
3.841ProVal: 3.841 ± 0.084
0.514ProTrp: 0.514 ± 0.031
1.835ProTyr: 1.835 ± 0.055
0.0ProXaa: 0.0 ± 0.0
Gln
5.743GlnAla: 5.743 ± 0.115
0.303GlnCys: 0.303 ± 0.022
1.956GlnAsp: 1.956 ± 0.063
2.588GlnGlu: 2.588 ± 0.076
1.671GlnPhe: 1.671 ± 0.062
3.081GlnGly: 3.081 ± 0.087
1.358GlnHis: 1.358 ± 0.046
1.798GlnIle: 1.798 ± 0.059
1.924GlnLys: 1.924 ± 0.064
6.236GlnLeu: 6.236 ± 0.129
0.876GlnMet: 0.876 ± 0.032
1.532GlnAsn: 1.532 ± 0.066
3.038GlnPro: 3.038 ± 0.077
3.538GlnGln: 3.538 ± 0.098
3.134GlnArg: 3.134 ± 0.08
2.155GlnSer: 2.155 ± 0.058
2.716GlnThr: 2.716 ± 0.077
3.834GlnVal: 3.834 ± 0.096
0.685GlnTrp: 0.685 ± 0.039
1.83GlnTyr: 1.83 ± 0.058
0.0GlnXaa: 0.0 ± 0.0
Arg
4.804ArgAla: 4.804 ± 0.081
0.461ArgCys: 0.461 ± 0.03
2.429ArgAsp: 2.429 ± 0.066
3.102ArgGlu: 3.102 ± 0.083
2.525ArgPhe: 2.525 ± 0.078
3.191ArgGly: 3.191 ± 0.077
1.351ArgHis: 1.351 ± 0.06
2.833ArgIle: 2.833 ± 0.076
2.385ArgLys: 2.385 ± 0.076
6.886ArgLeu: 6.886 ± 0.121
1.135ArgMet: 1.135 ± 0.05
2.015ArgAsn: 2.015 ± 0.062
2.878ArgPro: 2.878 ± 0.068
3.339ArgGln: 3.339 ± 0.086
3.985ArgArg: 3.985 ± 0.105
2.817ArgSer: 2.817 ± 0.073
3.682ArgThr: 3.682 ± 0.082
3.682ArgVal: 3.682 ± 0.089
0.89ArgTrp: 0.89 ± 0.035
2.479ArgTyr: 2.479 ± 0.072
0.0ArgXaa: 0.0 ± 0.0
Ser
5.647SerAla: 5.647 ± 0.102
0.635SerCys: 0.635 ± 0.037
2.614SerAsp: 2.614 ± 0.064
2.816SerGlu: 2.816 ± 0.062
3.081SerPhe: 3.081 ± 0.079
4.547SerGly: 4.547 ± 0.11
1.087SerHis: 1.087 ± 0.048
2.906SerIle: 2.906 ± 0.078
2.598SerLys: 2.598 ± 0.079
6.729SerLeu: 6.729 ± 0.12
1.207SerMet: 1.207 ± 0.05
2.463SerAsn: 2.463 ± 0.086
3.115SerPro: 3.115 ± 0.08
2.782SerGln: 2.782 ± 0.063
2.963SerArg: 2.963 ± 0.064
4.14SerSer: 4.14 ± 0.105
4.255SerThr: 4.255 ± 0.117
3.908SerVal: 3.908 ± 0.103
0.883SerTrp: 0.883 ± 0.04
2.449SerTyr: 2.449 ± 0.079
0.0SerXaa: 0.0 ± 0.0
Thr
6.494ThrAla: 6.494 ± 0.122
0.452ThrCys: 0.452 ± 0.03
3.369ThrAsp: 3.369 ± 0.075
2.93ThrGlu: 2.93 ± 0.078
2.469ThrPhe: 2.469 ± 0.075
5.523ThrGly: 5.523 ± 0.15
1.219ThrHis: 1.219 ± 0.045
3.392ThrIle: 3.392 ± 0.085
2.67ThrLys: 2.67 ± 0.08
7.025ThrLeu: 7.025 ± 0.134
1.184ThrMet: 1.184 ± 0.047
2.835ThrAsn: 2.835 ± 0.093
3.754ThrPro: 3.754 ± 0.084
2.88ThrGln: 2.88 ± 0.078
3.116ThrArg: 3.116 ± 0.073
4.457ThrSer: 4.457 ± 0.107
5.546ThrThr: 5.546 ± 0.157
4.595ThrVal: 4.595 ± 0.105
0.901ThrTrp: 0.901 ± 0.043
2.791ThrTyr: 2.791 ± 0.078
0.0ThrXaa: 0.0 ± 0.0
Val
7.411ValAla: 7.411 ± 0.131
0.564ValCys: 0.564 ± 0.032
2.981ValAsp: 2.981 ± 0.073
3.545ValGlu: 3.545 ± 0.078
2.593ValPhe: 2.593 ± 0.079
4.773ValGly: 4.773 ± 0.094
1.248ValHis: 1.248 ± 0.046
2.922ValIle: 2.922 ± 0.082
2.639ValLys: 2.639 ± 0.081
7.735ValLeu: 7.735 ± 0.121
1.07ValMet: 1.07 ± 0.047
2.385ValAsn: 2.385 ± 0.073
3.431ValPro: 3.431 ± 0.088
3.321ValGln: 3.321 ± 0.087
4.077ValArg: 4.077 ± 0.081
4.417ValSer: 4.417 ± 0.104
4.238ValThr: 4.238 ± 0.101
5.364ValVal: 5.364 ± 0.108
0.781ValTrp: 0.781 ± 0.035
2.394ValTyr: 2.394 ± 0.066
0.0ValXaa: 0.0 ± 0.0
Trp
1.16TrpAla: 1.16 ± 0.046
0.16TrpCys: 0.16 ± 0.016
0.58TrpAsp: 0.58 ± 0.032
0.612TrpGlu: 0.612 ± 0.036
0.564TrpPhe: 0.564 ± 0.029
0.853TrpGly: 0.853 ± 0.045
0.436TrpHis: 0.436 ± 0.029
0.454TrpIle: 0.454 ± 0.028
0.493TrpLys: 0.493 ± 0.03
2.024TrpLeu: 2.024 ± 0.063
0.247TrpMet: 0.247 ± 0.023
0.459TrpAsn: 0.459 ± 0.027
0.561TrpPro: 0.561 ± 0.033
1.02TrpGln: 1.02 ± 0.043
0.849TrpArg: 0.849 ± 0.044
0.699TrpSer: 0.699 ± 0.038
0.772TrpThr: 0.772 ± 0.037
0.968TrpVal: 0.968 ± 0.047
0.199TrpTrp: 0.199 ± 0.022
0.4TrpTyr: 0.4 ± 0.029
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.342TyrAla: 3.342 ± 0.085
0.459TyrCys: 0.459 ± 0.027
2.086TyrAsp: 2.086 ± 0.081
1.839TyrGlu: 1.839 ± 0.061
1.876TyrPhe: 1.876 ± 0.058
2.659TyrGly: 2.659 ± 0.078
0.902TyrHis: 0.902 ± 0.041
1.463TyrIle: 1.463 ± 0.049
1.612TyrLys: 1.612 ± 0.056
4.238TyrLeu: 4.238 ± 0.09
0.511TyrMet: 0.511 ± 0.032
1.696TyrAsn: 1.696 ± 0.066
1.732TyrPro: 1.732 ± 0.059
2.241TyrGln: 2.241 ± 0.068
2.424TyrArg: 2.424 ± 0.062
2.474TyrSer: 2.474 ± 0.066
2.38TyrThr: 2.38 ± 0.069
2.381TyrVal: 2.381 ± 0.063
0.687TyrTrp: 0.687 ± 0.032
1.908TyrTyr: 1.908 ± 0.065
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.002XaaLeu: 0.002 ± 0.002
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.002XaaThr: 0.002 ± 0.002
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2772 proteins (561870 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski