Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae; Chlorobaculum; Chlorobaculum tepidum

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2250 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8KC29|Q8KC29_CHLTE Dihydroorotate dehydrogenase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) OX=194439 GN=pyrDI PE=3 SV=1
MM1 pKa = 8.74LEE3 pKa = 3.75MCDD6 pKa = 3.37WEE8 pKa = 4.58EE9 pKa = 3.93NASGGGNFLRR19 pKa = 11.84SEE21 pKa = 4.14RR22 pKa = 11.84TEE24 pKa = 5.14LIDD27 pKa = 4.38SEE29 pKa = 4.67DD30 pKa = 3.49WPFVCSVAIGHH41 pKa = 5.63TVLYY45 pKa = 10.65

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2VFJ7|H2VFJ7_CHLTE Ribonuclease H OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) OX=194439 GN=rnhA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 8.89RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 10.04HH16 pKa = 4.5GFRR19 pKa = 11.84QRR21 pKa = 11.84MATKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 9.25VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.17GRR39 pKa = 11.84HH40 pKa = 4.97SLSVSSSMSASKK52 pKa = 10.59RR53 pKa = 3.53

Molecular weight: 6.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2250

0

2250

631563

30

1538

280.7

31.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.242 ± 0.061

1.147 ± 0.021

5.163 ± 0.039

6.698 ± 0.055

4.36 ± 0.039

7.582 ± 0.045

2.121 ± 0.024

6.287 ± 0.049

5.269 ± 0.048

9.949 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.024

3.412 ± 0.034

4.52 ± 0.034

3.128 ± 0.026

6.051 ± 0.052

6.304 ± 0.039

5.031 ± 0.037

7.067 ± 0.047

1.093 ± 0.021

2.805 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski