Thauera sp. 63

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae; Thauera; unclassified Thauera

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3951 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N6XFC0|N6XFC0_9RHOO 30S ribosomal protein S17 OS=Thauera sp. 63 OX=497321 GN=rpsQ PE=3 SV=1
MM1 pKa = 6.94SAEE4 pKa = 4.09VATPDD9 pKa = 3.22ILVFTDD15 pKa = 3.61SAANKK20 pKa = 8.85VRR22 pKa = 11.84EE23 pKa = 4.42LIEE26 pKa = 4.47EE27 pKa = 4.28EE28 pKa = 4.33GNPALKK34 pKa = 10.6LRR36 pKa = 11.84VFVSGGGCSGFQYY49 pKa = 10.93GFTFDD54 pKa = 4.28EE55 pKa = 4.93EE56 pKa = 4.59VNEE59 pKa = 5.57DD60 pKa = 3.3DD61 pKa = 3.6TTYY64 pKa = 11.03EE65 pKa = 4.12KK66 pKa = 11.07NGVMLLIDD74 pKa = 3.94PMSYY78 pKa = 10.31QYY80 pKa = 11.58LVGAEE85 pKa = 3.64IDD87 pKa = 3.75YY88 pKa = 10.77TEE90 pKa = 4.43GLEE93 pKa = 4.25GSQFVIRR100 pKa = 11.84NPNATSTCGCGSSFSAA116 pKa = 4.79

Molecular weight: 12.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N6YAY8|N6YAY8_9RHOO AsnC family transcriptional regulator OS=Thauera sp. 63 OX=497321 GN=C664_04857 PE=4 SV=1
MM1 pKa = 7.04NAKK4 pKa = 10.04HH5 pKa = 6.4PAQAPHH11 pKa = 6.19TPVVGAGRR19 pKa = 11.84RR20 pKa = 11.84SLQHH24 pKa = 6.51RR25 pKa = 11.84LGALRR30 pKa = 11.84AGLKK34 pKa = 9.83AALRR38 pKa = 11.84AFRR41 pKa = 11.84THH43 pKa = 6.86PNKK46 pKa = 10.4HH47 pKa = 5.04PQRR50 pKa = 5.23

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3951

0

3951

1270611

24

2207

321.6

35.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.765 ± 0.056

0.976 ± 0.014

5.55 ± 0.028

5.909 ± 0.037

3.591 ± 0.022

8.365 ± 0.042

2.292 ± 0.017

4.656 ± 0.027

2.973 ± 0.035

10.907 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.02

2.478 ± 0.024

5.075 ± 0.027

3.443 ± 0.021

7.586 ± 0.037

4.986 ± 0.024

4.86 ± 0.035

7.615 ± 0.036

1.388 ± 0.018

2.17 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski