Pedobacter antarcticus 4BY
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3836 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A081PCG8|A0A081PCG8_9SPHI Transcriptional regulator OS=Pedobacter antarcticus 4BY OX=1358423 GN=N180_01800 PE=4 SV=1
MM1 pKa = 7.1 EE2 pKa = 4.42 TTVQLALPEE11 pKa = 4.63 DD12 pKa = 4.75 FSTLCTIYY20 pKa = 10.39 QINPATLIQGFINQVFFPCFYY41 pKa = 10.91 SNPTGTDD48 pKa = 2.3 RR49 pKa = 11.84 WATYY53 pKa = 10.15 FFLNFLDD60 pKa = 4.06 VEE62 pKa = 4.14 EE63 pKa = 5.0 DD64 pKa = 3.25 AYY66 pKa = 10.63 EE67 pKa = 3.87 VDD69 pKa = 4.79 RR70 pKa = 11.84 EE71 pKa = 4.22 LEE73 pKa = 4.05 EE74 pKa = 4.47 PYY76 pKa = 10.96 LRR78 pKa = 11.84 IFNLRR83 pKa = 11.84 LLQLLDD89 pKa = 3.71 HH90 pKa = 7.33 DD91 pKa = 4.83 EE92 pKa = 4.62 EE93 pKa = 4.8 GTNAA97 pKa = 3.92
Molecular weight: 11.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.807
IPC2_protein 3.884
IPC_protein 3.795
Toseland 3.605
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.681
Rodwell 3.63
Grimsley 3.528
Solomon 3.745
Lehninger 3.694
Nozaki 3.897
DTASelect 4.037
Thurlkill 3.656
EMBOSS 3.694
Sillero 3.91
Patrickios 0.477
IPC_peptide 3.745
IPC2_peptide 3.884
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|A0A081PIP8|A0A081PIP8_9SPHI Uncharacterized protein OS=Pedobacter antarcticus 4BY OX=1358423 GN=N180_04930 PE=4 SV=1
MM1 pKa = 7.43 GVFGLLTAEE10 pKa = 4.26 YY11 pKa = 10.07 LPASLLTPMAAEE23 pKa = 4.36 LKK25 pKa = 10.31 VSEE28 pKa = 4.51 AVAGQAVTVTAIAALFSGLLLPGLTRR54 pKa = 11.84 SIDD57 pKa = 3.41 RR58 pKa = 11.84 RR59 pKa = 11.84 VVLLGFSALMIVSNLLVAFSSSIVVLLVMRR89 pKa = 11.84 TLLGVALGGFWAMTAAVAMRR109 pKa = 11.84 LVPVALVPRR118 pKa = 11.84 ALSIIFSGIAVGTVVSVPLGSYY140 pKa = 9.8 LGGMFGWRR148 pKa = 11.84 SAFYY152 pKa = 9.91 ASAGIGVVTLAFQWLTLPSLMPQRR176 pKa = 11.84 TVKK179 pKa = 10.04 TITVLQVLLRR189 pKa = 11.84 PGIAVGMLGCVLAHH203 pKa = 6.33 MGHH206 pKa = 6.22 YY207 pKa = 11.03 ALFTYY212 pKa = 10.13 IRR214 pKa = 11.84 PFLEE218 pKa = 3.98 STVGIGAEE226 pKa = 4.16 GLALILLGFGVANFAGTLLAGRR248 pKa = 11.84 LLEE251 pKa = 4.16 HH252 pKa = 6.63 SLRR255 pKa = 11.84 GTLIFMPVLVGVVALILVLIPSSFASHH282 pKa = 6.95 ALLIAIWGMAFGGVPVAWSAWVARR306 pKa = 11.84 AVPDD310 pKa = 3.5 QAEE313 pKa = 4.41 SAGGMVVASVQGAIAAGAAAGGLLFSFTGIVGVFVAAGISMVLAGLLIALWVRR366 pKa = 11.84 VQHH369 pKa = 6.28 PGVVQRR375 pKa = 5.01
Molecular weight: 38.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 9.823
IPC_protein 10.877
Toseland 10.526
ProMoST 10.891
Dawson 10.716
Bjellqvist 10.57
Wikipedia 11.038
Rodwell 10.643
Grimsley 10.818
Solomon 10.877
Lehninger 10.818
Nozaki 10.526
DTASelect 10.555
Thurlkill 10.584
EMBOSS 10.965
Sillero 10.657
Patrickios 10.292
IPC_peptide 10.877
IPC2_peptide 9.794
IPC2.peptide.svr19 8.545
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3836
0
3836
1283354
36
2171
334.6
37.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.404 ± 0.037
0.757 ± 0.011
5.249 ± 0.03
5.956 ± 0.039
4.942 ± 0.03
6.806 ± 0.036
1.822 ± 0.02
7.474 ± 0.039
6.994 ± 0.034
9.858 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.349 ± 0.017
5.401 ± 0.042
3.723 ± 0.022
3.895 ± 0.026
4.005 ± 0.023
6.524 ± 0.03
5.519 ± 0.029
6.24 ± 0.031
1.104 ± 0.015
3.977 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here