Candidatus Methylopumilus planktonicus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Methylophilaceae; Candidatus Methylopumilus

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1376 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D6EVT8|A0A0D6EVT8_9PROT Epimerase domain-containing protein OS=Candidatus Methylopumilus planktonicus OX=1581557 GN=BN1208_0926 PE=4 SV=1
MM1 pKa = 7.37TYY3 pKa = 10.71VVTEE7 pKa = 3.8NCIKK11 pKa = 10.53CKK13 pKa = 10.04YY14 pKa = 7.62TDD16 pKa = 4.08CVDD19 pKa = 3.61VCPVDD24 pKa = 4.53CFVEE28 pKa = 4.96GPNFLAINPDD38 pKa = 3.11EE39 pKa = 6.03CIDD42 pKa = 3.76CTLCVAEE49 pKa = 5.38CPAEE53 pKa = 4.5AIFAEE58 pKa = 4.66DD59 pKa = 5.19DD60 pKa = 3.86VPADD64 pKa = 3.33QQDD67 pKa = 4.35FIKK70 pKa = 10.71INADD74 pKa = 3.15MSKK77 pKa = 8.56IWPVISSRR85 pKa = 11.84KK86 pKa = 9.82DD87 pKa = 3.33PLPEE91 pKa = 4.8ADD93 pKa = 4.38SFNGKK98 pKa = 8.43PNKK101 pKa = 9.79RR102 pKa = 11.84SLLDD106 pKa = 3.31NN107 pKa = 4.31

Molecular weight: 11.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D6EXB7|A0A0D6EXB7_9PROT Tol-Pal system protein TolB OS=Candidatus Methylopumilus planktonicus OX=1581557 GN=tolB PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.31QPSKK9 pKa = 8.92VKK11 pKa = 10.35RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.74GFLVRR21 pKa = 11.84MKK23 pKa = 9.23TRR25 pKa = 11.84AGRR28 pKa = 11.84AVISARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84ARR41 pKa = 11.84IGLL44 pKa = 3.84

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1376

0

1376

420434

41

2982

305.5

34.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.302 ± 0.065

0.887 ± 0.021

5.396 ± 0.048

6.166 ± 0.068

4.587 ± 0.053

6.506 ± 0.093

2.341 ± 0.032

8.437 ± 0.067

7.824 ± 0.079

10.021 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.538 ± 0.028

4.915 ± 0.049

3.752 ± 0.034

3.561 ± 0.037

3.996 ± 0.047

6.758 ± 0.065

4.99 ± 0.054

5.97 ± 0.051

1.024 ± 0.025

3.029 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski