Streptomyces phage WRightOn

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Beephvirinae; Manuelvirus; Streptomyces virus WRightOn

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4PI92|A0A2H4PI92_9CAUD Uncharacterized protein OS=Streptomyces phage WRightOn OX=2053723 GN=SEA_WRIGHTON_54 PE=4 SV=1
MM1 pKa = 8.21DD2 pKa = 5.0KK3 pKa = 11.19LFVQNYY9 pKa = 9.02NNGEE13 pKa = 4.06YY14 pKa = 10.41TEE16 pKa = 4.88IDD18 pKa = 4.13LDD20 pKa = 4.42SAWMDD25 pKa = 3.29QDD27 pKa = 5.46VIDD30 pKa = 5.11ADD32 pKa = 4.07SLFQVLIVKK41 pKa = 10.41ISIWALEE48 pKa = 3.96HH49 pKa = 7.15GYY51 pKa = 10.31LLKK54 pKa = 10.89DD55 pKa = 3.1IVGFTLDD62 pKa = 3.79EE63 pKa = 4.76GSDD66 pKa = 3.88EE67 pKa = 5.04LGLPQTPGDD76 pKa = 4.18LTWLMEE82 pKa = 4.0VSKK85 pKa = 10.82LVAWRR90 pKa = 11.84HH91 pKa = 5.18TPTKK95 pKa = 10.59HH96 pKa = 5.69YY97 pKa = 9.74MPDD100 pKa = 3.34GALMARR106 pKa = 11.84INDD109 pKa = 3.78IGWKK113 pKa = 10.29FFDD116 pKa = 4.7FDD118 pKa = 4.56SDD120 pKa = 3.76LQEE123 pKa = 5.76AEE125 pKa = 4.62DD126 pKa = 4.33MYY128 pKa = 11.46SQEE131 pKa = 3.9FDD133 pKa = 3.03GDD135 pKa = 3.67YY136 pKa = 11.33AEE138 pKa = 4.46YY139 pKa = 10.64ARR141 pKa = 11.84EE142 pKa = 3.83YY143 pKa = 10.37MEE145 pKa = 4.38NCGEE149 pKa = 4.38GLSEE153 pKa = 3.72WAEE156 pKa = 3.77PYY158 pKa = 10.46FNYY161 pKa = 10.42EE162 pKa = 3.91EE163 pKa = 4.31YY164 pKa = 11.31GEE166 pKa = 4.7DD167 pKa = 4.57LVDD170 pKa = 4.52GYY172 pKa = 11.63DD173 pKa = 3.21RR174 pKa = 11.84VEE176 pKa = 4.13WDD178 pKa = 3.1NQEE181 pKa = 4.24YY182 pKa = 10.69LFHH185 pKa = 7.16RR186 pKa = 4.63

Molecular weight: 21.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4PI95|A0A2H4PI95_9CAUD DNA primase OS=Streptomyces phage WRightOn OX=2053723 GN=SEA_WRIGHTON_62 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.25KK3 pKa = 9.76IEE5 pKa = 4.15FNEE8 pKa = 3.84AFMGAGEE15 pKa = 4.11PLKK18 pKa = 10.67GSRR21 pKa = 11.84IPGFGAGNRR30 pKa = 11.84TPEE33 pKa = 3.86TSRR36 pKa = 11.84VHH38 pKa = 7.38RR39 pKa = 11.84SRR41 pKa = 11.84AMGKK45 pKa = 9.42VPAKK49 pKa = 9.96SVRR52 pKa = 11.84KK53 pKa = 9.4GLARR57 pKa = 11.84SCVSVKK63 pKa = 9.26WLNDD67 pKa = 2.58RR68 pKa = 11.84MAEE71 pKa = 4.02VRR73 pKa = 11.84VKK75 pKa = 10.01GTLHH79 pKa = 6.24ILGHH83 pKa = 5.66IRR85 pKa = 11.84VSKK88 pKa = 9.84GASGKK93 pKa = 9.88AYY95 pKa = 10.06SYY97 pKa = 11.44KK98 pKa = 10.37LVSEE102 pKa = 4.29QRR104 pKa = 11.84SHH106 pKa = 7.48NGFASQKK113 pKa = 9.43AAVDD117 pKa = 3.9RR118 pKa = 11.84MLEE121 pKa = 3.98KK122 pKa = 10.89CC123 pKa = 4.19

Molecular weight: 13.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13001

23

1644

203.1

22.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.761 ± 0.44

0.892 ± 0.167

6.661 ± 0.316

6.061 ± 0.257

3.338 ± 0.169

8.769 ± 0.369

1.592 ± 0.145

4.423 ± 0.194

5.661 ± 0.357

6.892 ± 0.193

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.938 ± 0.222

3.569 ± 0.194

4.469 ± 0.22

4.523 ± 0.316

5.338 ± 0.268

6.184 ± 0.307

6.584 ± 0.389

6.938 ± 0.348

1.723 ± 0.126

3.684 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski