Amino acid dipepetide frequency for Streptomyces phage WRightOn

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.077AlaAla: 11.077 ± 1.092
0.769AlaCys: 0.769 ± 0.247
5.077AlaAsp: 5.077 ± 0.777
6.462AlaGlu: 6.462 ± 0.626
2.615AlaPhe: 2.615 ± 0.403
9.154AlaGly: 9.154 ± 1.021
1.385AlaHis: 1.385 ± 0.267
3.385AlaIle: 3.385 ± 0.578
4.769AlaLys: 4.769 ± 0.586
7.385AlaLeu: 7.385 ± 0.813
2.615AlaMet: 2.615 ± 0.439
3.615AlaAsn: 3.615 ± 0.43
4.154AlaPro: 4.154 ± 0.667
5.154AlaGln: 5.154 ± 0.877
4.769AlaArg: 4.769 ± 0.592
6.308AlaSer: 6.308 ± 0.643
6.462AlaThr: 6.462 ± 0.793
6.538AlaVal: 6.538 ± 0.817
2.077AlaTrp: 2.077 ± 0.287
3.846AlaTyr: 3.846 ± 0.48
0.0AlaXaa: 0.0 ± 0.0
Cys
0.231CysAla: 0.231 ± 0.117
0.0CysCys: 0.0 ± 0.0
0.615CysAsp: 0.615 ± 0.22
0.538CysGlu: 0.538 ± 0.198
0.154CysPhe: 0.154 ± 0.124
0.846CysGly: 0.846 ± 0.318
0.231CysHis: 0.231 ± 0.142
0.231CysIle: 0.231 ± 0.181
0.538CysLys: 0.538 ± 0.256
0.692CysLeu: 0.692 ± 0.264
0.231CysMet: 0.231 ± 0.159
0.154CysAsn: 0.154 ± 0.108
0.769CysPro: 0.769 ± 0.328
0.462CysGln: 0.462 ± 0.173
0.538CysArg: 0.538 ± 0.232
0.462CysSer: 0.462 ± 0.169
0.692CysThr: 0.692 ± 0.301
1.154CysVal: 1.154 ± 0.341
0.154CysTrp: 0.154 ± 0.127
0.231CysTyr: 0.231 ± 0.171
0.0CysXaa: 0.0 ± 0.0
Asp
6.923AspAla: 6.923 ± 0.65
0.692AspCys: 0.692 ± 0.245
4.462AspAsp: 4.462 ± 0.606
4.077AspGlu: 4.077 ± 0.652
2.231AspPhe: 2.231 ± 0.399
5.308AspGly: 5.308 ± 0.549
0.923AspHis: 0.923 ± 0.214
4.231AspIle: 4.231 ± 0.501
2.846AspLys: 2.846 ± 0.402
4.538AspLeu: 4.538 ± 0.632
2.462AspMet: 2.462 ± 0.589
1.846AspAsn: 1.846 ± 0.401
4.692AspPro: 4.692 ± 0.653
2.538AspGln: 2.538 ± 0.496
3.385AspArg: 3.385 ± 0.531
3.692AspSer: 3.692 ± 0.622
3.692AspThr: 3.692 ± 0.482
4.385AspVal: 4.385 ± 0.573
1.462AspTrp: 1.462 ± 0.309
2.231AspTyr: 2.231 ± 0.399
0.0AspXaa: 0.0 ± 0.0
Glu
6.077GluAla: 6.077 ± 0.523
0.769GluCys: 0.769 ± 0.364
3.615GluAsp: 3.615 ± 0.467
3.538GluGlu: 3.538 ± 0.478
2.231GluPhe: 2.231 ± 0.356
5.077GluGly: 5.077 ± 0.569
0.846GluHis: 0.846 ± 0.239
3.692GluIle: 3.692 ± 0.516
2.769GluLys: 2.769 ± 0.483
5.692GluLeu: 5.692 ± 0.56
1.692GluMet: 1.692 ± 0.332
1.769GluAsn: 1.769 ± 0.363
2.077GluPro: 2.077 ± 0.433
2.538GluGln: 2.538 ± 0.421
3.385GluArg: 3.385 ± 0.4
3.385GluSer: 3.385 ± 0.47
3.385GluThr: 3.385 ± 0.586
4.308GluVal: 4.308 ± 0.754
0.923GluTrp: 0.923 ± 0.271
2.846GluTyr: 2.846 ± 0.528
0.0GluXaa: 0.0 ± 0.0
Phe
3.077PheAla: 3.077 ± 0.626
0.231PheCys: 0.231 ± 0.13
2.692PheAsp: 2.692 ± 0.423
2.615PheGlu: 2.615 ± 0.492
1.154PhePhe: 1.154 ± 0.215
2.846PheGly: 2.846 ± 0.585
0.769PheHis: 0.769 ± 0.244
1.0PheIle: 1.0 ± 0.206
1.231PheLys: 1.231 ± 0.303
1.769PheLeu: 1.769 ± 0.387
0.769PheMet: 0.769 ± 0.217
1.615PheAsn: 1.615 ± 0.291
2.0PhePro: 2.0 ± 0.427
1.923PheGln: 1.923 ± 0.334
1.769PheArg: 1.769 ± 0.428
2.231PheSer: 2.231 ± 0.351
2.308PheThr: 2.308 ± 0.401
1.846PheVal: 1.846 ± 0.352
0.231PheTrp: 0.231 ± 0.126
1.308PheTyr: 1.308 ± 0.282
0.0PheXaa: 0.0 ± 0.0
Gly
7.769GlyAla: 7.769 ± 0.83
1.0GlyCys: 1.0 ± 0.287
5.231GlyAsp: 5.231 ± 0.621
6.077GlyGlu: 6.077 ± 0.594
3.231GlyPhe: 3.231 ± 0.488
8.538GlyGly: 8.538 ± 0.995
1.308GlyHis: 1.308 ± 0.345
3.923GlyIle: 3.923 ± 0.624
5.692GlyLys: 5.692 ± 0.719
6.154GlyLeu: 6.154 ± 0.673
2.923GlyMet: 2.923 ± 0.412
3.077GlyAsn: 3.077 ± 0.643
2.769GlyPro: 2.769 ± 0.353
3.923GlyGln: 3.923 ± 0.456
3.769GlyArg: 3.769 ± 0.503
6.231GlySer: 6.231 ± 0.509
5.385GlyThr: 5.385 ± 0.878
5.615GlyVal: 5.615 ± 0.651
1.692GlyTrp: 1.692 ± 0.305
3.385GlyTyr: 3.385 ± 0.677
0.0GlyXaa: 0.0 ± 0.0
His
1.154HisAla: 1.154 ± 0.268
0.231HisCys: 0.231 ± 0.164
1.231HisAsp: 1.231 ± 0.292
0.692HisGlu: 0.692 ± 0.201
0.692HisPhe: 0.692 ± 0.2
1.692HisGly: 1.692 ± 0.342
0.308HisHis: 0.308 ± 0.168
0.769HisIle: 0.769 ± 0.258
0.538HisLys: 0.538 ± 0.21
1.077HisLeu: 1.077 ± 0.23
0.462HisMet: 0.462 ± 0.155
0.615HisAsn: 0.615 ± 0.25
0.462HisPro: 0.462 ± 0.198
0.385HisGln: 0.385 ± 0.176
1.0HisArg: 1.0 ± 0.241
1.077HisSer: 1.077 ± 0.287
1.077HisThr: 1.077 ± 0.304
1.308HisVal: 1.308 ± 0.333
0.308HisTrp: 0.308 ± 0.185
0.846HisTyr: 0.846 ± 0.276
0.0HisXaa: 0.0 ± 0.0
Ile
3.0IleAla: 3.0 ± 0.427
0.538IleCys: 0.538 ± 0.234
4.077IleAsp: 4.077 ± 0.625
2.769IleGlu: 2.769 ± 0.31
1.154IlePhe: 1.154 ± 0.353
3.154IleGly: 3.154 ± 0.401
1.231IleHis: 1.231 ± 0.284
1.846IleIle: 1.846 ± 0.305
3.385IleLys: 3.385 ± 0.63
3.462IleLeu: 3.462 ± 0.374
1.308IleMet: 1.308 ± 0.404
1.923IleAsn: 1.923 ± 0.385
1.846IlePro: 1.846 ± 0.383
1.538IleGln: 1.538 ± 0.315
2.846IleArg: 2.846 ± 0.456
1.846IleSer: 1.846 ± 0.339
3.462IleThr: 3.462 ± 0.553
2.923IleVal: 2.923 ± 0.453
0.385IleTrp: 0.385 ± 0.148
1.538IleTyr: 1.538 ± 0.364
0.0IleXaa: 0.0 ± 0.0
Lys
6.154LysAla: 6.154 ± 0.787
0.615LysCys: 0.615 ± 0.207
3.385LysAsp: 3.385 ± 0.62
2.615LysGlu: 2.615 ± 0.489
2.462LysPhe: 2.462 ± 0.428
4.077LysGly: 4.077 ± 0.565
1.154LysHis: 1.154 ± 0.253
2.385LysIle: 2.385 ± 0.376
3.846LysLys: 3.846 ± 0.571
4.385LysLeu: 4.385 ± 0.646
1.615LysMet: 1.615 ± 0.322
2.692LysAsn: 2.692 ± 0.542
2.538LysPro: 2.538 ± 0.452
2.0LysGln: 2.0 ± 0.415
2.846LysArg: 2.846 ± 0.512
3.154LysSer: 3.154 ± 0.363
2.615LysThr: 2.615 ± 0.393
5.077LysVal: 5.077 ± 0.728
1.077LysTrp: 1.077 ± 0.243
1.846LysTyr: 1.846 ± 0.491
0.0LysXaa: 0.0 ± 0.0
Leu
6.154LeuAla: 6.154 ± 0.913
0.231LeuCys: 0.231 ± 0.14
5.077LeuAsp: 5.077 ± 0.795
3.846LeuGlu: 3.846 ± 0.492
2.692LeuPhe: 2.692 ± 0.486
5.154LeuGly: 5.154 ± 0.628
0.692LeuHis: 0.692 ± 0.221
3.615LeuIle: 3.615 ± 0.442
4.538LeuLys: 4.538 ± 0.606
4.154LeuLeu: 4.154 ± 0.545
1.308LeuMet: 1.308 ± 0.381
2.692LeuAsn: 2.692 ± 0.483
2.692LeuPro: 2.692 ± 0.457
4.077LeuGln: 4.077 ± 0.529
4.769LeuArg: 4.769 ± 0.683
4.923LeuSer: 4.923 ± 0.539
3.769LeuThr: 3.769 ± 0.668
5.077LeuVal: 5.077 ± 0.733
0.923LeuTrp: 0.923 ± 0.276
2.538LeuTyr: 2.538 ± 0.455
0.0LeuXaa: 0.0 ± 0.0
Met
4.692MetAla: 4.692 ± 0.567
0.154MetCys: 0.154 ± 0.098
2.0MetAsp: 2.0 ± 0.357
1.769MetGlu: 1.769 ± 0.349
0.615MetPhe: 0.615 ± 0.172
3.154MetGly: 3.154 ± 0.51
0.231MetHis: 0.231 ± 0.128
1.308MetIle: 1.308 ± 0.257
1.538MetLys: 1.538 ± 0.418
1.077MetLeu: 1.077 ± 0.353
0.923MetMet: 0.923 ± 0.264
1.385MetAsn: 1.385 ± 0.301
1.615MetPro: 1.615 ± 0.354
0.846MetGln: 0.846 ± 0.256
1.692MetArg: 1.692 ± 0.29
2.0MetSer: 2.0 ± 0.456
2.0MetThr: 2.0 ± 0.378
1.615MetVal: 1.615 ± 0.317
0.385MetTrp: 0.385 ± 0.142
0.385MetTyr: 0.385 ± 0.156
0.0MetXaa: 0.0 ± 0.0
Asn
3.538AsnAla: 3.538 ± 0.557
0.538AsnCys: 0.538 ± 0.194
1.769AsnAsp: 1.769 ± 0.37
1.769AsnGlu: 1.769 ± 0.316
1.231AsnPhe: 1.231 ± 0.257
3.231AsnGly: 3.231 ± 0.578
0.615AsnHis: 0.615 ± 0.2
1.538AsnIle: 1.538 ± 0.328
2.385AsnLys: 2.385 ± 0.47
3.231AsnLeu: 3.231 ± 0.507
0.846AsnMet: 0.846 ± 0.238
1.077AsnAsn: 1.077 ± 0.253
2.462AsnPro: 2.462 ± 0.46
1.846AsnGln: 1.846 ± 0.352
1.923AsnArg: 1.923 ± 0.363
1.846AsnSer: 1.846 ± 0.424
2.538AsnThr: 2.538 ± 0.339
1.385AsnVal: 1.385 ± 0.289
0.692AsnTrp: 0.692 ± 0.213
1.231AsnTyr: 1.231 ± 0.299
0.0AsnXaa: 0.0 ± 0.0
Pro
4.692ProAla: 4.692 ± 0.508
0.462ProCys: 0.462 ± 0.206
3.231ProAsp: 3.231 ± 0.398
3.077ProGlu: 3.077 ± 0.416
1.846ProPhe: 1.846 ± 0.391
4.538ProGly: 4.538 ± 0.67
0.462ProHis: 0.462 ± 0.179
1.538ProIle: 1.538 ± 0.399
2.385ProLys: 2.385 ± 0.419
2.769ProLeu: 2.769 ± 0.36
1.231ProMet: 1.231 ± 0.31
1.308ProAsn: 1.308 ± 0.281
1.692ProPro: 1.692 ± 0.415
1.154ProGln: 1.154 ± 0.337
2.077ProArg: 2.077 ± 0.384
3.0ProSer: 3.0 ± 0.447
3.692ProThr: 3.692 ± 0.49
3.231ProVal: 3.231 ± 0.39
0.538ProTrp: 0.538 ± 0.24
1.769ProTyr: 1.769 ± 0.347
0.0ProXaa: 0.0 ± 0.0
Gln
5.462GlnAla: 5.462 ± 0.649
0.231GlnCys: 0.231 ± 0.127
2.769GlnAsp: 2.769 ± 0.35
2.538GlnGlu: 2.538 ± 0.602
1.385GlnPhe: 1.385 ± 0.309
3.077GlnGly: 3.077 ± 0.47
0.692GlnHis: 0.692 ± 0.187
2.0GlnIle: 2.0 ± 0.35
2.154GlnLys: 2.154 ± 0.445
3.385GlnLeu: 3.385 ± 0.528
1.538GlnMet: 1.538 ± 0.453
1.231GlnAsn: 1.231 ± 0.271
2.0GlnPro: 2.0 ± 0.476
2.385GlnGln: 2.385 ± 0.519
3.462GlnArg: 3.462 ± 0.745
2.154GlnSer: 2.154 ± 0.322
3.154GlnThr: 3.154 ± 0.482
2.923GlnVal: 2.923 ± 0.403
0.846GlnTrp: 0.846 ± 0.262
1.846GlnTyr: 1.846 ± 0.345
0.0GlnXaa: 0.0 ± 0.0
Arg
5.923ArgAla: 5.923 ± 0.68
0.692ArgCys: 0.692 ± 0.264
3.846ArgAsp: 3.846 ± 0.43
3.769ArgGlu: 3.769 ± 0.626
1.692ArgPhe: 1.692 ± 0.407
4.538ArgGly: 4.538 ± 0.453
1.0ArgHis: 1.0 ± 0.25
2.077ArgIle: 2.077 ± 0.348
2.846ArgLys: 2.846 ± 0.574
4.154ArgLeu: 4.154 ± 0.659
2.077ArgMet: 2.077 ± 0.381
1.769ArgAsn: 1.769 ± 0.426
1.538ArgPro: 1.538 ± 0.283
2.308ArgGln: 2.308 ± 0.402
2.385ArgArg: 2.385 ± 0.47
2.923ArgSer: 2.923 ± 0.602
3.462ArgThr: 3.462 ± 0.39
4.308ArgVal: 4.308 ± 0.502
0.769ArgTrp: 0.769 ± 0.243
1.308ArgTyr: 1.308 ± 0.256
0.0ArgXaa: 0.0 ± 0.0
Ser
5.462SerAla: 5.462 ± 0.74
0.385SerCys: 0.385 ± 0.212
4.231SerAsp: 4.231 ± 0.594
3.846SerGlu: 3.846 ± 0.636
1.462SerPhe: 1.462 ± 0.275
6.462SerGly: 6.462 ± 1.044
1.0SerHis: 1.0 ± 0.229
3.154SerIle: 3.154 ± 0.469
2.769SerLys: 2.769 ± 0.582
3.538SerLeu: 3.538 ± 0.457
1.615SerMet: 1.615 ± 0.361
2.692SerAsn: 2.692 ± 0.387
2.615SerPro: 2.615 ± 0.436
2.538SerGln: 2.538 ± 0.328
3.231SerArg: 3.231 ± 0.476
4.692SerSer: 4.692 ± 0.89
3.769SerThr: 3.769 ± 0.626
4.769SerVal: 4.769 ± 0.558
1.077SerTrp: 1.077 ± 0.294
2.538SerTyr: 2.538 ± 0.478
0.0SerXaa: 0.0 ± 0.0
Thr
5.308ThrAla: 5.308 ± 0.758
0.538ThrCys: 0.538 ± 0.27
4.154ThrAsp: 4.154 ± 0.609
3.0ThrGlu: 3.0 ± 0.464
2.538ThrPhe: 2.538 ± 0.351
7.154ThrGly: 7.154 ± 0.982
1.308ThrHis: 1.308 ± 0.296
3.0ThrIle: 3.0 ± 0.521
4.462ThrLys: 4.462 ± 0.735
4.0ThrLeu: 4.0 ± 0.603
2.154ThrMet: 2.154 ± 0.417
1.846ThrAsn: 1.846 ± 0.392
4.385ThrPro: 4.385 ± 0.585
3.462ThrGln: 3.462 ± 0.457
2.308ThrArg: 2.308 ± 0.367
3.615ThrSer: 3.615 ± 0.881
5.231ThrThr: 5.231 ± 0.932
3.923ThrVal: 3.923 ± 0.586
1.231ThrTrp: 1.231 ± 0.263
2.231ThrTyr: 2.231 ± 0.447
0.0ThrXaa: 0.0 ± 0.0
Val
5.615ValAla: 5.615 ± 0.669
0.538ValCys: 0.538 ± 0.227
5.615ValAsp: 5.615 ± 0.508
4.0ValGlu: 4.0 ± 0.69
2.154ValPhe: 2.154 ± 0.456
4.769ValGly: 4.769 ± 0.581
1.462ValHis: 1.462 ± 0.378
2.462ValIle: 2.462 ± 0.409
4.538ValLys: 4.538 ± 0.549
3.923ValLeu: 3.923 ± 0.513
2.154ValMet: 2.154 ± 0.311
2.231ValAsn: 2.231 ± 0.347
2.308ValPro: 2.308 ± 0.494
3.769ValGln: 3.769 ± 0.661
4.308ValArg: 4.308 ± 0.791
5.538ValSer: 5.538 ± 0.545
5.231ValThr: 5.231 ± 0.789
4.385ValVal: 4.385 ± 0.73
1.462ValTrp: 1.462 ± 0.283
2.923ValTyr: 2.923 ± 0.399
0.0ValXaa: 0.0 ± 0.0
Trp
1.692TrpAla: 1.692 ± 0.365
0.077TrpCys: 0.077 ± 0.069
1.308TrpAsp: 1.308 ± 0.32
1.308TrpGlu: 1.308 ± 0.315
0.615TrpPhe: 0.615 ± 0.187
1.0TrpGly: 1.0 ± 0.254
0.0TrpHis: 0.0 ± 0.0
0.692TrpIle: 0.692 ± 0.205
1.385TrpLys: 1.385 ± 0.307
1.154TrpLeu: 1.154 ± 0.275
0.462TrpMet: 0.462 ± 0.21
0.385TrpAsn: 0.385 ± 0.149
0.615TrpPro: 0.615 ± 0.245
0.692TrpGln: 0.692 ± 0.225
1.231TrpArg: 1.231 ± 0.297
0.615TrpSer: 0.615 ± 0.217
1.462TrpThr: 1.462 ± 0.355
1.308TrpVal: 1.308 ± 0.268
0.385TrpTrp: 0.385 ± 0.163
0.846TrpTyr: 0.846 ± 0.304
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.615TyrAla: 3.615 ± 0.586
0.231TyrCys: 0.231 ± 0.117
2.231TyrAsp: 2.231 ± 0.446
2.308TyrGlu: 2.308 ± 0.461
1.231TyrPhe: 1.231 ± 0.296
3.923TyrGly: 3.923 ± 0.464
0.308TyrHis: 0.308 ± 0.171
1.385TyrIle: 1.385 ± 0.291
2.0TyrLys: 2.0 ± 0.344
2.385TyrLeu: 2.385 ± 0.384
0.923TyrMet: 0.923 ± 0.314
1.769TyrAsn: 1.769 ± 0.355
1.462TyrPro: 1.462 ± 0.343
1.692TyrGln: 1.692 ± 0.317
1.692TyrArg: 1.692 ± 0.29
2.154TyrSer: 2.154 ± 0.377
2.462TyrThr: 2.462 ± 0.479
3.308TyrVal: 3.308 ± 0.42
0.615TyrTrp: 0.615 ± 0.196
1.154TyrTyr: 1.154 ± 0.25
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (13001 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski