Alistipes indistinctus YIT 12060
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2348 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G5H5X6|G5H5X6_9BACT Uncharacterized protein OS=Alistipes indistinctus YIT 12060 OX=742725 GN=HMPREF9450_00336 PE=4 SV=1
MM1 pKa = 7.64 KK2 pKa = 10.09 KK3 pKa = 10.31 YY4 pKa = 9.98 KK5 pKa = 10.64 CEE7 pKa = 3.96 VCGYY11 pKa = 9.71 IYY13 pKa = 10.59 DD14 pKa = 4.25 PAVGDD19 pKa = 3.97 PDD21 pKa = 4.21 NGIEE25 pKa = 4.26 PGTPFEE31 pKa = 5.37 DD32 pKa = 4.77 LPEE35 pKa = 4.74 DD36 pKa = 3.81 WVCPVCQEE44 pKa = 3.83 GKK46 pKa = 10.36 EE47 pKa = 4.17 VFEE50 pKa = 4.56 EE51 pKa = 4.52 VV52 pKa = 3.24
Molecular weight: 5.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.855
IPC2_protein 3.986
IPC_protein 3.834
Toseland 3.668
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.681
Grimsley 3.592
Solomon 3.783
Lehninger 3.745
Nozaki 3.948
DTASelect 4.062
Thurlkill 3.719
EMBOSS 3.732
Sillero 3.961
Patrickios 0.401
IPC_peptide 3.783
IPC2_peptide 3.935
IPC2.peptide.svr19 3.868
Protein with the highest isoelectric point:
>tr|G5H6Y4|G5H6Y4_9BACT Ribosome-binding factor A OS=Alistipes indistinctus YIT 12060 OX=742725 GN=rbfA PE=3 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.39 KK6 pKa = 9.62 RR7 pKa = 11.84 KK8 pKa = 6.72 RR9 pKa = 11.84 HH10 pKa = 5.17 KK11 pKa = 10.05 MATHH15 pKa = 6.02 KK16 pKa = 10.34 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.3 NRR25 pKa = 11.84 HH26 pKa = 5.61 KK27 pKa = 10.81 KK28 pKa = 9.9 NKK30 pKa = 9.46
Molecular weight: 3.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.023
IPC_protein 12.544
Toseland 12.735
ProMoST 13.203
Dawson 12.735
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.705
Grimsley 12.764
Solomon 13.217
Lehninger 13.115
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.427
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 8.989
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2348
0
2348
832160
21
2245
354.4
39.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.573 ± 0.048
1.16 ± 0.019
5.487 ± 0.036
6.039 ± 0.046
4.246 ± 0.032
7.798 ± 0.04
1.842 ± 0.023
6.195 ± 0.044
5.058 ± 0.04
9.366 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.601 ± 0.02
4.082 ± 0.034
4.464 ± 0.031
3.352 ± 0.023
5.878 ± 0.045
5.966 ± 0.037
5.726 ± 0.038
6.989 ± 0.041
1.211 ± 0.025
3.968 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here