Alistipes indistinctus YIT 12060 
Average proteome isoelectric point is 6.57 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 2348 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|G5H5X6|G5H5X6_9BACT Uncharacterized protein OS=Alistipes indistinctus YIT 12060 OX=742725 GN=HMPREF9450_00336 PE=4 SV=1 
MM1 pKa = 7.64  KK2 pKa = 10.09  KK3 pKa = 10.31  YY4 pKa = 9.98  KK5 pKa = 10.64  CEE7 pKa = 3.96  VCGYY11 pKa = 9.71  IYY13 pKa = 10.59  DD14 pKa = 4.25  PAVGDD19 pKa = 3.97  PDD21 pKa = 4.21  NGIEE25 pKa = 4.26  PGTPFEE31 pKa = 5.37  DD32 pKa = 4.77  LPEE35 pKa = 4.74  DD36 pKa = 3.81  WVCPVCQEE44 pKa = 3.83  GKK46 pKa = 10.36  EE47 pKa = 4.17  VFEE50 pKa = 4.56  EE51 pKa = 4.52  VV52 pKa = 3.24   
 Molecular weight: 5.87 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.855 
IPC2_protein 3.986 
IPC_protein 3.834 
Toseland    3.668 
ProMoST     3.986 
Dawson      3.808 
Bjellqvist  3.973 
Wikipedia   3.719 
Rodwell     3.681 
Grimsley    3.592 
Solomon     3.783 
Lehninger   3.745 
Nozaki      3.948 
DTASelect   4.062 
Thurlkill   3.719 
EMBOSS      3.732 
Sillero     3.961 
Patrickios  0.401 
IPC_peptide 3.783 
IPC2_peptide  3.935 
IPC2.peptide.svr19  3.868 
 Protein with the highest isoelectric point: 
>tr|G5H6Y4|G5H6Y4_9BACT Ribosome-binding factor A OS=Alistipes indistinctus YIT 12060 OX=742725 GN=rbfA PE=3 SV=1 
MM1 pKa = 7.84  PSGKK5 pKa = 9.39  KK6 pKa = 9.62  RR7 pKa = 11.84  KK8 pKa = 6.72  RR9 pKa = 11.84  HH10 pKa = 5.17  KK11 pKa = 10.05  MATHH15 pKa = 6.02  KK16 pKa = 10.34  RR17 pKa = 11.84  KK18 pKa = 9.84  KK19 pKa = 9.28  RR20 pKa = 11.84  LRR22 pKa = 11.84  KK23 pKa = 9.3  NRR25 pKa = 11.84  HH26 pKa = 5.61  KK27 pKa = 10.81  KK28 pKa = 9.9  NKK30 pKa = 9.46   
 Molecular weight: 3.79 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.447 
IPC2_protein 11.023 
IPC_protein 12.544 
Toseland    12.735 
ProMoST     13.203 
Dawson      12.735 
Bjellqvist  12.705 
Wikipedia   13.188 
Rodwell     12.705 
Grimsley    12.764 
Solomon     13.217 
Lehninger   13.115 
Nozaki      12.72 
DTASelect   12.705 
Thurlkill   12.72 
EMBOSS      13.217 
Sillero     12.72 
Patrickios  12.427 
IPC_peptide 13.217 
IPC2_peptide  12.193 
IPC2.peptide.svr19  8.989 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        2348 
 
        
        0
 
        
        2348 
         
        832160
 
        21
 
        2245
 
        354.4
 
        39.44
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        8.573 ± 0.048
1.16 ± 0.019
 
        5.487 ± 0.036
6.039 ± 0.046
 
        4.246 ± 0.032
7.798 ± 0.04
 
        1.842 ± 0.023
6.195 ± 0.044
       
        5.058 ± 0.04
9.366 ± 0.053
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.601 ± 0.02
4.082 ± 0.034
 
        4.464 ± 0.031
3.352 ± 0.023
 
        5.878 ± 0.045
5.966 ± 0.037
 
        5.726 ± 0.038
6.989 ± 0.041
       
        1.211 ± 0.025
3.968 ± 0.039
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here