Pepper vein yellows virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 7.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3Y1T5|I3Y1T5_9LUTE Readthrough protein OS=Pepper vein yellows virus 2 OX=2560636 PE=3 SV=1
MM1 pKa = 7.85LEE3 pKa = 4.17VEE5 pKa = 4.53SSDD8 pKa = 3.37MSAEE12 pKa = 4.07EE13 pKa = 3.96LVQAGLCDD21 pKa = 4.92PIRR24 pKa = 11.84TFVKK28 pKa = 10.42RR29 pKa = 11.84EE30 pKa = 3.58PHH32 pKa = 5.86KK33 pKa = 10.47QSKK36 pKa = 10.11LDD38 pKa = 3.38EE39 pKa = 4.27GRR41 pKa = 11.84YY42 pKa = 8.96RR43 pKa = 11.84LIMSVSLVDD52 pKa = 3.45QLVARR57 pKa = 11.84VLFQNQNKK65 pKa = 9.66RR66 pKa = 11.84EE67 pKa = 3.96IALWRR72 pKa = 11.84ANPSKK77 pKa = 10.61PGFGLSTDD85 pKa = 3.63EE86 pKa = 4.0QVLEE90 pKa = 4.39FVQALAAQVEE100 pKa = 4.75VPPEE104 pKa = 3.73EE105 pKa = 5.59VITSWEE111 pKa = 4.24KK112 pKa = 10.58YY113 pKa = 9.87LVPTDD118 pKa = 3.67CSGFDD123 pKa = 3.21WSVAEE128 pKa = 4.57WMLHH132 pKa = 5.89DD133 pKa = 5.83DD134 pKa = 3.43MVVRR138 pKa = 11.84NKK140 pKa = 9.41LTLDD144 pKa = 4.03LNPTTEE150 pKa = 4.13KK151 pKa = 10.71LRR153 pKa = 11.84SVWLKK158 pKa = 9.68CICNSVLCLSDD169 pKa = 3.31GTLLAQRR176 pKa = 11.84VPGVQKK182 pKa = 10.45SGSYY186 pKa = 8.25NTSSSNSRR194 pKa = 11.84IRR196 pKa = 11.84VMAAYY201 pKa = 9.95HH202 pKa = 6.59CGADD206 pKa = 2.88WAMAMGDD213 pKa = 3.72DD214 pKa = 4.39ALEE217 pKa = 4.26SVNTNLEE224 pKa = 4.09VYY226 pKa = 10.15KK227 pKa = 10.94DD228 pKa = 3.51LGFKK232 pKa = 10.78VEE234 pKa = 4.14VSGQLEE240 pKa = 4.4FCSHH244 pKa = 6.9IFRR247 pKa = 11.84APDD250 pKa = 2.96LALPVNEE257 pKa = 4.57RR258 pKa = 11.84KK259 pKa = 8.59MLYY262 pKa = 10.45KK263 pKa = 10.76LIFGYY268 pKa = 10.86NPGSGNLEE276 pKa = 4.07VISNYY281 pKa = 9.46IAACVSVLNEE291 pKa = 4.24LRR293 pKa = 11.84HH294 pKa = 6.43DD295 pKa = 4.37PDD297 pKa = 3.73SVALLHH303 pKa = 5.94QWLVSPVLPQNNN315 pKa = 3.37

Molecular weight: 35.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1A0W5|E1A0W5_9LUTE Movement protein OS=Pepper vein yellows virus 2 OX=2560636 PE=3 SV=2
MM1 pKa = 6.55NTEE4 pKa = 3.97GVRR7 pKa = 11.84RR8 pKa = 11.84NNNGNGGSRR17 pKa = 11.84GTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84PRR26 pKa = 11.84QVRR29 pKa = 11.84PVVVVAPPGRR39 pKa = 11.84TRR41 pKa = 11.84RR42 pKa = 11.84GNRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84SGGRR52 pKa = 11.84NRR54 pKa = 11.84NRR56 pKa = 11.84VGGRR60 pKa = 11.84SSNSEE65 pKa = 3.69TFIFNKK71 pKa = 10.42DD72 pKa = 3.59SIKK75 pKa = 10.74DD76 pKa = 3.69SSSGTVTFGPSLSEE90 pKa = 4.37SIALSGGVLKK100 pKa = 10.54AYY102 pKa = 10.22HH103 pKa = 6.68EE104 pKa = 4.5YY105 pKa = 10.51KK106 pKa = 9.32ITMVNIRR113 pKa = 11.84FVSEE117 pKa = 4.1SSSTAEE123 pKa = 3.6GSIAYY128 pKa = 9.39EE129 pKa = 4.08LDD131 pKa = 3.39PHH133 pKa = 7.02CKK135 pKa = 8.88LTSLQSTLRR144 pKa = 11.84KK145 pKa = 9.77FPVTKK150 pKa = 10.36GGQATFRR157 pKa = 11.84ASQINGVEE165 pKa = 3.87WHH167 pKa = 6.98DD168 pKa = 3.52TSEE171 pKa = 4.07DD172 pKa = 3.6QFRR175 pKa = 11.84LLYY178 pKa = 10.2KK179 pKa = 10.92GNGTKK184 pKa = 10.41GVAAGFFQIRR194 pKa = 11.84FTVQLHH200 pKa = 5.06NPKK203 pKa = 10.54

Molecular weight: 22.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2273

153

699

378.8

42.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.379 ± 0.72

1.452 ± 0.364

4.179 ± 0.916

6.115 ± 0.277

3.784 ± 0.397

7.479 ± 0.695

2.156 ± 0.444

3.696 ± 0.23

6.071 ± 0.694

8.447 ± 1.284

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.716 ± 0.279

4.663 ± 0.635

6.467 ± 0.752

3.784 ± 0.158

6.775 ± 0.979

9.855 ± 0.548

6.027 ± 0.739

6.467 ± 0.704

1.804 ± 0.192

2.64 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski